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公开(公告)号：US08367597B2
公开(公告)日：2013-02-05
申请号：US13213583
申请日：2011-08-19
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译： 本文公开了一种在具有羧酸酯的制剂中稳定CE-7酯酶的过水解酶活性的方法，其使用向CE-7酯酶和羧酸的混合物中添加基本上未溶解的缓冲剂 酯。 此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。
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公开(公告)号：US08337905B2
公开(公告)日：2012-12-25
申请号：US12572107
申请日：2009-10-01
申请人： Robert Dicosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
发明人： Robert Dicosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are multi-component peroxycarboxylic acid generation systems for enzymatically producing aqueous formulations of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid generation systems comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.
摘要翻译： 本文公开了用于酶促生产适用于例如消毒剂和/或漂白应用的过氧羧酸的水性制剂的多组分过氧羧酸生成系统。 多组分过氧羧酸生成系统包含至少一种具有过水解活性的碳水化合物酯酶家族7酶。
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63.发明申请PRODUCTION OF ALCOHOL ESTERS AND IN SITU PRODUCT REMOVAL DURING ALCOHOL FERMENTATION 有权在酒精发酵期间生产酒精产品和脱除产品公开(公告)号：US20120208246A1
公开(公告)日：2012-08-16
申请号：US13193147
申请日：2011-07-28
申请人： Douglas Robert Anton , Stephane Francois Bazzana , Keith H. Burlew , Jelena Cirakovic , Robert Dicosimo , Bruce A. Diner , Michael Charles Grady , Brian Michael Roesch , Francis J. Woerner
发明人： Douglas Robert Anton , Stephane Francois Bazzana , Keith H. Burlew , Jelena Cirakovic , Robert Dicosimo , Bruce A. Diner , Michael Charles Grady , Brian Michael Roesch , Francis J. Woerner
CPC分类号： A23K1/007 , A23K10/12 , A23K10/38 , C12P7/16 , C12P7/62 , C12P7/649 , C12P2203/00 , Y02E50/10 , Y02E50/13 , Y02P60/873
摘要： An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium.
摘要翻译： 醇发酵方法和组合物，其包括通过在发酵培养基中与产物醇与羧酸（例如，脂肪酸）和能够酯化产物醇（例如丁醇）的催化剂（例如脂肪酶）酯化产生醇酯， 与羧酸形成醇酯。 醇酯可以从发酵培养基中提取，并从醇酯中回收产物醇。 羧酸也可以用作从发酵培养基中除去醇酯的提取剂。
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公开(公告)号：US20110305683A1
公开(公告)日：2011-12-15
申请号：US13213642
申请日：2011-08-19
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US07919288B2
公开(公告)日：2011-04-05
申请号：US12429563
申请日：2009-04-24
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
摘要翻译： 提供了一种在水性反应条件下将乙醇腈转化成乙醇酸时，提高具有腈水解酶活性的酶催化剂的比活性的方法。 包含有效量的至少一种胺保护剂提高了酶催化剂的比活性和催化生产率。
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公开(公告)号：US20100196975A1
公开(公告)日：2010-08-05
申请号：US12429554
申请日：2009-04-24
IPC分类号： C12P7/56
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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公开(公告)号：US20090111162A1
公开(公告)日：2009-04-30
申请号：US11931069
申请日：2007-10-31
IPC分类号： C12N9/78
CPC分类号： C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
摘要： A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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公开(公告)号：US20080045754A1
公开(公告)日：2008-02-21
申请号：US11818393
申请日：2007-06-13
申请人： Michael D'Amore , Leo Manzer , Edward Miller , Robert Dicosimo , Jeffrey Knapp
发明人： Michael D'Amore , Leo Manzer , Edward Miller , Robert Dicosimo , Jeffrey Knapp
CPC分类号： C07C41/06 , C07C1/24 , C07C2/14 , C07C2/62 , C07C2/70 , C07C5/03 , C07C9/21 , C07C29/04 , C07C11/08 , C07C31/12 , C07C31/125 , C07C43/04
摘要： The present invention relates to a process for making butenes using dry 1-butanol obtained from fermentation broth. The butenes so produced may be converted to isoalkanes, alkyl-substituted aromatics, isooctanes, isooctanols, and octyl ethers, which are useful in transportation fuels.
摘要翻译： 本发明涉及使用从发酵液获得的干燥的1-丁醇制备丁烯的方法。 如此生产的丁烯可以转化为可用于运输燃料的异烷烃，烷基取代的芳族化合物，异辛烷，异辛醇和辛基醚。
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69.发明申请公开(公告)号：US20060189821A1
公开(公告)日：2006-08-24
申请号：US11315708
申请日：2005-12-21
申请人： F. Gallagher , Robert Dicosimo
发明人： F. Gallagher , Robert Dicosimo
IPC分类号： C07C67/03
CPC分类号： C07C67/08 , C07C51/09 , Y02P20/582 , C07C69/675 , C07C59/06 , C07C59/08 , C07C69/42 , C07C69/68 , C07C69/14 , C07C69/44 , C07C69/80 , C07C69/82 , C07C69/24
摘要： The present invention relates to a method for obtaining a carboxylic acid ester from an aqueous solution of the corresponding carboxylic acid ammonium salt using alcoholysis. The recovered carboxylic acid ester may be subsequently hydrolyzed to produce the corresponding carboxylic acid.
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70.发明授权公开(公告)号：US06368804B1
公开(公告)日：2002-04-09
申请号：US09614914
申请日：2000-07-12
IPC分类号： C12N120
摘要： A method for preserving immobilized or unimmobilized microbial cells having nitrilase activity and for stabilizing the nitrilase activity of unimmobilized or immobilized microbial cells has been developed. The unimmobilized or immobilized microbial cells are stored in an aqueous solution containing from about 0.10 M to the saturation concentration of an inorganic salt of bicarbonate or carbonate, including ammonium, sodium and potassium salts of bicarbonate or carbonate. Aqueous suspensions containing at least 100 mM bicarbonate or carbonate limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the uninmmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include Acidovorax facilis 72-PF-15 (ATCC 55747), Acidovorax facilis 72-PF-17 (ATCC 55745), Acidovorax facilis 72W (ATCC 55746), and transformed microbial cells having nitrilase activity, preferably E. coli SS1001 (ATCC PTA-1177) which is transformed with Acidovorax facilis 72W nitrilase activity.
摘要翻译： 已经开发了用于保存具有腈水解酶活性的固定或未固化的微生物细胞并稳定未固定或固定的微生物细胞的腈水解酶活性的方法。 将未固定或固定的微生物细胞储存在含有约0.10M的水溶液中，使饱和浓度为碳酸氢盐或碳酸盐的无机盐，包括碳酸氢盐或碳酸盐的铵，钠和钾盐。 含有至少100mM碳酸氢盐或碳酸氢盐的水性悬浮液限制所储存的酶催化剂的微生物污染物，以及稳定未固定化或固定的细胞的所需腈水解酶活性。 其特征在于腈水解酶活性并通过该方法稳定和保存的微生物包括Acidovorax facilis 72-PF-15（ATCC 55747），Acidovorax facilis 72-PF-17（ATCC 55745），Acidovorax facilis 72W（ATCC 55746）和 转化的具有腈水解酶活性的微生物细胞，优选用Acidovorax facilis 72W腈水解酶活性转化的大肠杆菌SS1001（ATCC PTA-1177）。
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