公开(公告)号：US20220073565A1

公开(公告)日：2022-03-10

申请号：US17419413

申请日：2020-01-06

Applicant: University of Washington ,  The Regents of the University of California

Inventor： Robert LANGAN ,  Andrew NG ,  Scott BOYKEN ,  Marc LAJOIE ,  Hana EL-SAMAD ,  David BAKER

IPC: C07K14/00

Abstract: Disclosed herein are non-naturally occurring cage polypeptides, kits and degron LOCKRs including the cage polypep-tides, and uses thereof, wherein the cage polypeptides include (a) a helical bundle, comprising between 2 and 7 alpha-helices, wherein the helical bundle includes: (i) a structural region; and (ii) a latch region, wherein the latch region composes a degron located within the latch region, wherein the structural region interacts with the latch region to prevent activity of the degron; and (b) amino acid linkers connecting each alpha helix

公开(公告)号：US20220025356A1

公开(公告)日：2022-01-27

申请号：US17480078

申请日：2021-09-20

Applicant: University of Washington

Inventor： David BAKER ,  David YOUNGER ,  Eric KLAVINS

IPC: C12N15/10

Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

公开(公告)号：US20210101945A1

公开(公告)日：2021-04-08

申请号：US16993975

申请日：2020-08-14

Applicant: UNIVERSITY OF WASHINGTON

Inventor： David BAKER ,  Scott BOYKEN ,  Zibo CHEN ,  Chunfu XU ,  Sherry BERMEO ,  Robert LANGAN

IPC: C07K14/435 ,  C07K1/113 ,  C07K14/00 ,  G16B15/20 ,  C07K2/00 ,  C07K16/18

Abstract: Methods and apparatus for identifying and screening hydrogen bond networks are provided. A computing device can determine a search space for hydrogen bond networks related to one or more molecules, where the search space can include a plurality of energy terms related to a plurality of residues related to the hydrogen bond networks. The computing device can search the search space to identify one or more hydrogen bond networks based on the plurality of energy terms. The computing device can screen the identified hydrogen bond networks to identity one or more screened hydrogen bond networks based on scores for the identified hydrogen bond networks. An output can be generated that is related to the one or more screened hydrogen bond networks. Also provided are polypeptides that can form homo-oligomers with modular hydrogen bond network-mediated specificity.

公开(公告)号：US20200224186A1

公开(公告)日：2020-07-16

申请号：US16676253

申请日：2019-11-06

Applicant: UNIVERSITY OF WASHINGTON ,  UNIVERSITY OF UTAH

Inventor： Neil KING ,  Wesley SUNDQUIST ,  Joerg VOTTELER ,  Yang HSIA ,  David BAKER ,  Jacob BALE ,  Marc LAJOIE ,  Gabriel BUTTERFIELD ,  Elizabeth GRAY ,  Daniel STETSON

IPC: C12N9/88 ,  C07K14/00 ,  C07K14/435

Abstract: The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.

公开(公告)号：US20190341124A1

公开(公告)日：2019-11-07

申请号：US16271571

申请日：2019-02-08

Applicant: University of Washington through its center for commercialization

Inventor： David BAKER ,  Neil KING ,  Jacob BALE ,  William SHEFFLER

IPC: G16B20/00 ,  G16B15/00 ,  G16B5/00 ,  C07K14/195 ,  G01N33/68 ,  C07K14/00

Abstract: Synthetic nanostructures, proteins that are useful, for example, in making synthetic nanostructures, and methods for designing such synthetic nanostructures are disclosed herein.

公开(公告)号：US20170082624A1

公开(公告)日：2017-03-23

申请号：US15272650

申请日：2016-09-22

Applicant: University of Washington

Inventor： Paul YAGER ,  Caitlin ANDERSON ,  David BAKER ,  Yu-Ru LIN ,  Carly HOLSTEIN

IPC: G01N33/569 ,  C07K14/00

CPC classification number: G01N33/56983 ,  C07K14/00 ,  C07K14/001 ,  C07K2319/43 ,  C07K2319/70 ,  G01N2333/11

Abstract: The present invention provides porous membrane-binding polypeptides, fusion proteins thereof, and methods for use of the polypeptides and fusion proteins in binding assays.

公开(公告)号：US20160024155A1

公开(公告)日：2016-01-28

申请号：US14877348

申请日：2015-10-07

Applicant: University of Washington through its Center for Commercialization

Inventor： David BAKER ,  Timothy A. WHITEHEAD ,  Sarel FLEISHMAN

IPC: C07K7/08 ,  G01N33/569 ,  A61K38/16 ,  A61K38/10 ,  C07K14/00

CPC classification number: C07K7/08 ,  A61K38/00 ,  A61K38/10 ,  A61K38/16 ,  C07K14/00 ,  C07K14/001 ,  G01N33/53 ,  G01N33/56983 ,  G01N2333/11 ,  G01N2469/10

Abstract: Polypeptides are disclosed herein, which recognize and are strong binders to Influenza A hemagglutinin and can be used, for example, to treat and/or limit development of an influenza infection.

公开(公告)号：US20150376246A1

公开(公告)日：2015-12-31

申请号：US14766259

申请日：2014-03-13

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： David BAKER ,  Christine E. TINBERG ,  Sagar D. KHARE ,  Jiayi DOU

IPC: C07K14/435 ,  G01N33/74

CPC classification number: C07K14/435 ,  A61K38/005 ,  C12N15/1034 ,  G01N33/743

Abstract: Isolated polypeptides with steroid binding activity and methods for their use as therapeutics and detection agents are disclosed herein.

Abstract translation: 具有类固醇结合活性的分离的多肽及其用作治疗剂和检测剂的方法在本文中公开。

公开(公告)号：US20150356240A1

公开(公告)日：2015-12-10

申请号：US14759308

申请日：2014-02-07

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： David BAKER ,  Neil KING ,  Jacob BALE ,  William SHEFFLER

IPC: G06F19/18 ,  G06F19/12 ,  G06F19/16 ,  C07K14/00 ,  G01N33/68

CPC classification number: G06F19/18 ,  B82Y5/00 ,  C07K14/00 ,  C07K14/195 ,  C07K2319/735 ,  G01N33/6845 ,  G01N2570/00 ,  G06F19/12 ,  G06F19/16

Abstract: Synthetic nanostructures, proteins that are useful, for example, in making synthetic nanostructures, and methods for designing such synthetic nanostructures are disclosed herein.

Abstract translation: 本文公开了合成纳米结构，可用于制备合成纳米结构的蛋白质，以及用于设计这种合成纳米结构的方法。

公开(公告)号：US20250154213A1

公开(公告)日：2025-05-15

申请号：US18945961

申请日：2024-11-13

Applicant: University of Washington

Inventor： Hsien-Wei YEH ,  David BAKER

IPC: C07K14/47 ,  A61K38/00 ,  G01N33/53 ,  G01N33/74

Abstract: Polypeptides are disclosed having an amino acid sequence at least 50% identical to the amino acid sequence of SEQ ID NO:1, wherein, relative to SEQ ID NO:1, residue 43 is I, residue 95 is Q, and residue 128 is L, fusion proteins thereof, kits thereof, and methods for using the polypeptides for treating a disorder associated with cortisol, or for detecting cortisol in a biological sample.