公开(公告)号：US20090203046A1

公开(公告)日：2009-08-13

申请号：US11813743

申请日：2006-02-01

申请人： Yuichi Ono ,  Tomoya Nakatani

发明人： Yuichi Ono ,  Tomoya Nakatani

IPC分类号： G01N33/567 ,  C07H21/02 ,  C12N15/74 ,  C07K14/00 ,  C07K16/00 ,  C12P21/00

CPC分类号： C12Q1/6881 ,  C07K14/47 ,  C07K16/18 ,  C12Q2600/158 ,  G01N33/56966

摘要： Total RNAs were prepared from the ventral and dorsal regions of embryonic day 12.5 mouse mesencephalon, and a cDNA fragment was obtained by the subtraction method (N-RDA). The full-length cDNA was cloned and the nucleotide sequence was determined. The gene was named Corl2. The result of homology search showed that about 850 residues of Corl2 exhibited homology to the XM_355050 sequence deposited in GenBank; however, the remaining ˜150 residues showed no homology. Thus, Corl2 was demonstrated to be a novel gene. The Corl2 expression in various mouse organs was analyzed by RT-PCR, and the result showed that Corl2 expression was specific to brain. The result of immunostaining day 12.5 embryos and postnatal day 12 brains demonstrated that Corl2 is useful as a Purkinje cell marker at any differentiation stages.

摘要翻译： 从胚胎第12.5天小鼠中脑的腹侧和背部区域制备总RNA，通过减法法（N-RDA）获得cDNA片段。 克隆全长cDNA，测定核苷酸序列。 该基因命名为Corl2。 同源性检索结果表明，Corl2的约850个残基与存在于GenBank中的XM_355050序列具有同源性; 然而，剩余的〜150个残基显示没有同源性。 因此，Corl2被证明是一种新的基因。 通过RT-PCR分析各种小鼠器官中的Corl2表达，结果表明Corl2表达对脑有特异性。 免疫染色第12.5天胚胎和出生后12天脑的结果表明，Corl2在任何分化阶段用作浦肯野细胞标记。

公开(公告)号：US20090152104A1

公开(公告)日：2009-06-18

申请号：US12067670

申请日：2006-06-21

申请人： Yuichi Ono ,  Masanori Yamaguchi

发明人： Yuichi Ono ,  Masanori Yamaguchi

IPC分类号： C25C3/08 ,  C25C3/12 ,  C25C3/28 ,  C25C3/00 ,  C25C3/26

CPC分类号： C25C3/02 ,  C25C7/005 ,  C25C7/04

摘要： A molten salt electrolyzer for reducing metal comprises an electrolytic cell filled with a molten salt composed of a reducing metal chloride, an anode immersed in the molten salt of the electrolytic cell and surrounded by a first wall at the periphery thereof, and a cathode immersed in the molten salt of the electrolytic cell and surrounded by a second wall at the periphery thereof.

摘要翻译： 用于还原金属的熔融盐电解器包括填充有由还原金属氯化物组成的熔融盐的电解池，浸在电解槽的熔融盐中并被其周边的第一壁包围的阳极和浸入 电解池的熔融盐，并在周边由第二壁包围。

公开(公告)号：US20080199437A1

公开(公告)日：2008-08-21

申请号：US10552485

申请日：2005-07-22

申请人： Yoshimasa Sakamoto ,  Yuichi Ono ,  Toshio Imai ,  Yasuko Nakagawa

发明人： Yoshimasa Sakamoto ,  Yuichi Ono ,  Toshio Imai ,  Yasuko Nakagawa

IPC分类号： A61K35/12 ,  C07H21/04 ,  C12N5/06 ,  C12Q1/68 ,  A61P25/16 ,  C07K17/02 ,  C12Q1/02 ,  C07K16/18

CPC分类号： C12N5/0623 ,  A61K35/30 ,  C07K16/286 ,  C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/5023 ,  G01N33/56966 ,  G01N33/6896 ,  G01N2500/00 ,  G01N2800/2835

摘要： The present invention relates to polynucleotide probes and antibodies for detecting Lrp4/Corin dopaminergic neuron progenitor cell markers, which enable the efficient separation of dopaminergic neuron progenitor cells; and methods for selecting the progenitor cells by the use thereof.

摘要翻译： 本发明涉及用于检测Lrp4 / Corin多巴胺能神经元祖细胞标记物的多核苷酸探针和抗体，其能够有效分离多巴胺能神经元祖细胞; 以及通过其使用来选择祖细胞的方法。

公开(公告)号：US20080119641A1

公开(公告)日：2008-05-22

申请号：US11522043

申请日：2006-09-15

申请人： Miyuki Nishimura ,  Mayumi Asano ,  Yuichi Ono ,  Koji Morimoto ,  Masakazu Takeuchi ,  Yoko Inoue ,  Toshio Imai ,  Yoshimi Takai

发明人： Miyuki Nishimura ,  Mayumi Asano ,  Yuichi Ono ,  Koji Morimoto ,  Masakazu Takeuchi ,  Yoko Inoue ,  Toshio Imai ,  Yoshimi Takai

IPC分类号： C07K16/18

CPC分类号： C07K14/47 ,  C07K14/705

摘要： A mouse cDNA library from gene fragments encoding proteins localizing at cell—cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.

摘要翻译： 通过技术可视化蛋白质的定位技术筛选编码在细胞连接处定位的蛋白质的蛋白质的基因片段的小鼠cDNA文库，以鉴定连接蛋白和相关蛋白JEAP。 GenBank同源性检索是根据序列进行的。 基于获得的序列，筛选小鼠cDNA文库以鉴定JEAP-2。 通过使用针对这些蛋白质的制备的抗体，显示这些蛋白质在紧密连接处特别是在外分泌腺中的紧密连接处特异性表达。

公开(公告)号：US20080053838A1

公开(公告)日：2008-03-06

申请号：US11576891

申请日：2005-10-05

申请人： Masanori Yamaguchi ,  Yuichi Ono ,  Susumu Kosemura ,  Eiji Nishimura ,  Tadashi Ogasawara ,  Makoto Yamaguchi ,  Masahiko Hori ,  Toru Uenishi

发明人： Masanori Yamaguchi ,  Yuichi Ono ,  Susumu Kosemura ,  Eiji Nishimura ,  Tadashi Ogasawara ,  Makoto Yamaguchi ,  Masahiko Hori ,  Toru Uenishi

IPC分类号： C25C3/28

CPC分类号： C25C3/02 ,  C22B34/129 ,  C25B1/26 ,  C25C3/28

摘要： A method for production of metal by molten-salt electrolysis is a method for production of metal by molten-salt electrolysis which is performed by filling molten salt of a metal chloride in an electrolysis vessel having an anode and a cathode, and a molten salt which reduces solubility of the metal in the molten salt is used.

摘要翻译： 通过熔盐电解生产金属的方法是通过熔盐电解生产金属的方法，其通过在具有阳极和阴极的电解容器中填充金属氯化物的熔融盐和熔融盐来进行， 使用熔融盐中金属的溶解度降低。

公开(公告)号：US20070254281A1

公开(公告)日：2007-11-01

申请号：US10580989

申请日：2004-11-26

申请人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

发明人： Yuichi Ono ,  Yasuko Nakagawa ,  Tomoya Nakatani

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/6896 ,  G01N33/9413 ,  G01N2800/2835

摘要： The present invention identified Lmx1a genes, which are expressed in dopaminergic neurons at all differentiation stages, from proliferating dopaminergic neuron progenitor cells before cell cycle exit to cells after cell cycle exit. Lmx1a expression in cells can be used as an indicator when selecting cells suitable for transplantation therapy for neurodegenerative diseases such as Parkinson's disease, and is useful as a marker for screening agents involved in the induction of dopaminergic neuron differentiation.

摘要翻译： 本发明鉴定了在细胞周期退出至细胞周期后出现的细胞周期性增殖的多巴胺能神经元祖细胞中，在所有分化阶段的多巴胺能神经元中表达的Lmx1a基因。 当选择适合于帕金森病等神经变性疾病的移植治疗的细胞时，细胞中的Lmx1a表达可用作指示剂，并且可用作参与多巴胺能神经元分化诱导的筛选剂的标记。

公开(公告)号：US5799765A

公开(公告)日：1998-09-01

申请号：US207116

申请日：1994-03-07

申请人： Yuichi Ono ,  Mitsuaki Hatori ,  Hiroshi Inuoe

发明人： Yuichi Ono ,  Mitsuaki Hatori ,  Hiroshi Inuoe

IPC分类号： F16D35/02 ,  F16D35/00

CPC分类号： F16D35/027

摘要： A fluid clutch comprises a driving disc rotationally driven by a driving section, a rotatable casing enhousing the driving disc, a blower attached to the casing, and an oil supply means for supplying an oil from an outside to a torque transmission gap between the driving disc and the casing. Further, a driving section may be connected by means of a flexible joint to either one of the casing or the disc. The fluid clutch can always attain optimum control by adjusting the amount of the oil at a high accuracy and rapidly depending on various kinds of operation conditions. A breezer also is provided for releasing gas from the torque transmission chamber when pressure exceeds a predetermined upper limit, and for enabling inflow of atmospheric air to the torque transmission chamber when pressure is less than a predetermined lower limit. The clutch is endurable to external vibrations.

摘要翻译： 流体离合器包括由驱动部旋转驱动的驱动盘，安装驱动盘的可旋转壳体，附接到壳体的鼓风机和用于将油从外部供给到驱动盘之间的转矩传递间隙的供油装置 和套管。 此外，驱动部分可以通过柔性接头连接到壳体或盘中的任一个。 流体离合器可以通过根据各种操作条件高精度和快速地调节油量来始终获得最佳控制。 另外，当压力超过规定的上限时，也设置有用于从转矩传递室释放气体的微风机，并且当压力小于预定的下限时能够将大气输送到转矩传递室。 离合器可耐受外部振动。

公开(公告)号：US4919247A

公开(公告)日：1990-04-24

申请号：US275766

申请日：1988-11-23

申请人： Kazunori Takikawa ,  Yuichi Ono

发明人： Kazunori Takikawa ,  Yuichi Ono

IPC分类号： F16D35/00 ,  F04D25/02 ,  F16D35/02

CPC分类号： F16D35/022 ,  F04D25/022

摘要： A fluidized fan coupling device is provided to substantially eliminate accompanying rotation followed engine start up. The device includes a sealed housing with a partition plate dividing the interior of the housing into a torque transfer chamber and an oil reservoir. The housing is rotatably mounted to a shaft which extends into the torque transfer chamber. A driving disk is mounted to the shaft and is disposed in the torque transfer chamber. An oil outflow regulating hole extends through the partition plate and is provided with a valve that responds to changes in temperature. The partition plate also has an oil circulating aperture to permit flow of oil from the torque transfer chamber to the oil reservoir only when the engine is stopped. An idle oil groove in the outer periphery of the torque transfer chamber communicates with an oil circulation passage which extends from the idle oil groove to the oil reservoir. A dam is provided in the idle oil groove to urge the oil through the oil circulation passage. Thus, a portion of the oil accumulated in the torque transfer chamber flows through the oil circulating aperture after the engine has stopped. Any remaining oil is accumulated in the idle oil groove and is urged by the dam through the oil circulating passage immediately after engine start up to substantially eliminate accompanying rotation.

摘要翻译： 提供流化风扇联接装置，以便在发动机起动之后基本上消除伴随旋转。 该装置包括密封壳体，其具有将壳体的内部分成转矩传递室和储油器的隔板。 壳体可旋转地安装到延伸到转矩传递室中的轴上。 驱动盘安装在轴上并设置在转矩传递室中。 油流出调节孔延伸穿过隔板，并设置有响应于温度变化的阀。 分隔板还具有油循环孔，以允许仅当发动机停止时才将油从转矩传递室流出到油储存器。 转矩传递室外周的怠速油槽与从怠速油槽延伸到储油部的油循环通路连通。 在怠速油槽中设置有一个坝，以将油通过油循环通道。 因此，在发动机停止后，积存在转矩传递室中的一部分油流过油循环孔。 任何剩余的油都积聚在怠速油槽中，并且在发动机起动之后立即通过油循环通道被大坝推动，从而基本上消除了伴随旋转。

公开(公告)号：US4903643A

公开(公告)日：1990-02-27

申请号：US244698

申请日：1988-09-14

申请人： Kazunori Takikawa ,  Yuichi Ono

发明人： Kazunori Takikawa ,  Yuichi Ono

IPC分类号： F16D35/02 ,  F01P7/02

CPC分类号： F16D35/022

摘要： A temperature-sensing fan fluid coupling for use with an automobile engine. The coupling comprises a sealed enclosure, a partition plate having an outflow control hole, a thermostat mounted upon the front surface of the enclosure, a rodlike link member in contact with the thermostat at its one end, an interlocking member against which the link member bears, and a resilient tonguelike valve member. One end of the valve member is fixed, while the other end is in resilient contact with the front end of the interlocking member. The thermostat is connected to the valve member via the link member and the interlocking member. As the ambient temperature changes, the thermostat deforms to cause the valve member to more or less open the hole.

摘要翻译： 一种用于汽车发动机的温度感应风扇流体联轴器。 联接器包括密封的外壳，具有流出控制孔的分隔板，安装在外壳前表面上的恒温器，与其一端与恒温器接触的杆状连杆构件，连杆构件所承载的互锁构件 ，以及弹性舌形阀构件。 阀构件的一端固定，而另一端与互锁构件的前端弹性接触。 恒温器通过连杆构件和互锁构件连接到阀构件。 随着环境温度的变化，恒温器变形，导致阀门构件或多或少打开孔。

公开(公告)号：US4850465A

公开(公告)日：1989-07-25

申请号：US122936

申请日：1987-11-19

申请人： Yuichi Ono

发明人： Yuichi Ono

IPC分类号： F16D35/00 ,  F16D1/00 ,  F16D35/02

CPC分类号： F16D35/026

摘要： A temperature-sensitive fan-coupling apparatus is provided with a driving side and a driven side. The driving side comprises a tightly sealed housing. The driven side comprises a rotary member from which fan blades extend. The rotary member includes a following disc disposed in the housing with a torque transmission gap existing between the following disc and the housing. A partition plate is mounted to the rotary member to define an oil reservoir therebetween. An aperture extends through the partition plate to provide communication between the oil reservoir and the torque transmission gap. A temperature-sensitive member is mounted external to the driven end of the apparatus and to one end of a connecting rod which extends through the rotary member and into the oil reservoir. A valve member is mounted to the end of the connecting rod in the oil reservoir and is disposed to selectively cover or open the hole in the partition plate to control the flow of fluid between the oil reservoir and the torque transmission gap. Movement of the valve member is controlled by the temperature-sensitive member.

摘要翻译： 温度敏感的风扇联接装置设置有驱动侧和从动侧。 驱动侧包括紧密密封的壳体。 从动侧包括风扇叶片延伸的旋转构件。 旋转构件包括设置在壳体中的随动盘，其中存在于随后的盘和壳体之间的扭矩传递间隙。 分隔板安装到旋转构件上以在其间限定油储存器。 孔径延伸穿过隔板以提供储油器与扭矩传递间隙之间的连通。 温度敏感部件安装在装置的从动端的外部，并且连接杆的一端延伸穿过旋转部件并进入储油器。 阀构件安装在储油器中的连杆的端部，并且设置成选择性地覆盖或打开隔板中的孔，以控制储油器和扭矩传递间隙之间的流体流动。 阀构件的移动由温度敏感构件控制。