公开(公告)号：US10428359B2

公开(公告)日：2019-10-01

申请号：US15717042

申请日：2017-09-27

申请人： AJINOMOTO CO., INC.

发明人： Mika Moriya ,  Kazuyuki Hayashi ,  Akari Yokokawa

IPC分类号： C12P13/20 ,  C07C227/28 ,  C12P13/08 ,  C12P13/14 ,  C07K14/34 ,  C12N9/88 ,  C07C229/36 ,  C12N1/20 ,  C12N15/66 ,  C12Q1/686

摘要： A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability, which has been modified so that the activity of a C4-dicarboxylic acid-uptake carrier such as DctA, DcuA, and DcuB is increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.

公开(公告)号：US20190277862A1

公开(公告)日：2019-09-12

申请号：US16239817

申请日：2019-01-04

申请人： AJINOMOTO CO., INC.

发明人： Takeshi IKEUCHI ,  Nobuhiro KAWAI ,  Yuki YANO ,  Naoko ARASHIDA ,  Rumi NISHIMOTO ,  Kazutaka SHIMBO

IPC分类号： G01N33/68

摘要： An evaluating method includes an evaluating step of evaluating a state of mild cognitive impairment or Alzheimer's disease for a subject to be evaluated using a concentration value of at least one of α-ABA, Ala, Arg, Asn, Cit, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Orn, Phe, Pro, Ser, Thr, Trp, Tyr, Val, Cysteine, Taurine, GABA, Hypotaurine, S-Adenosyl homocysteine, Sarcosine, Serotonin, Spermidine, hCit, 3-hKyn, aAiBA, N8-Acetylspermidine, Phosphoserine, bABA, ADMA, and Thioproline in blood of the subject.

公开(公告)号：US20190264191A1

公开(公告)日：2019-08-29

申请号：US16287459

申请日：2019-02-27

申请人： AJINOMOTO CO., INC.

发明人： Ayako SATO ,  Eri HIGASHIURA ,  Misato OKAMOTO ,  Takayuki ITO ,  Erika WATANABE ,  Uno TAGAMI ,  Yuki ODA ,  Tatsuki KASHIWAGI ,  Masayuki SUGIKI

IPC分类号： C12N9/00 ,  C12P21/02

摘要： A mutant glutathione synthetase (GSHB) suitable for generating γ-Glu-Val-Gly, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val-Gly is produced by using a mutant GSHB having a mutation at such a position as V7, N13, I14, N15, K17, F22, F95, M165, N199, Y200, P202, I274, T285, and P287.

公开(公告)号：US20190249205A1

公开(公告)日：2019-08-15

申请号：US16392756

申请日：2019-04-24

申请人： AJINOMOTO CO., INC.

发明人： Benjamin Mijts ,  Christine Roche ,  Peter Kelly ,  Sayaka Asari ,  Keita Fukui ,  Miku Toyazaki

IPC分类号： C12P7/24 ,  C12N1/20 ,  C12N9/10 ,  C12N9/88 ,  C12N9/02 ,  C12N9/04

CPC分类号： C12P7/24 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0069 ,  C12N9/0073 ,  C12N9/1007 ,  C12N9/1085 ,  C12N9/88 ,  C12P7/22 ,  C12P7/42 ,  C12Y101/01001 ,  C12Y101/01025 ,  C12Y102/99007 ,  C12Y113/11003 ,  C12Y114/13082 ,  C12Y201/01015 ,  C12Y205/01054 ,  C12Y402/0101 ,  C12Y402/01118 ,  C12Y402/03004

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of an L-cysteine biosynthesis enzyme is increased.

公开(公告)号：US20190241914A1

公开(公告)日：2019-08-08

申请号：US16392776

申请日：2019-04-24

申请人： AJINOMOTO CO., INC.

发明人： Benjamin Mijts ,  Christine Roche ,  Peter Kelly ,  Sayaka Asari ,  Miku Toyazaki ,  Keita Fukui

IPC分类号： C12P7/42

CPC分类号： C12P7/42 ,  C12P7/22 ,  C12P7/24 ,  C12P13/001 ,  C12P13/12 ,  C12P17/10 ,  C12P19/40

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of NCgl2048 protein is reduced.

公开(公告)号：US10369163B2

公开(公告)日：2019-08-06

申请号：US15093207

申请日：2016-04-07

申请人： AJINOMOTO CO., INC.

发明人： Tetsuro Shibakusa ,  Mayu Sugita

IPC分类号： A61K31/675 ,  A61K31/4172 ,  A61K31/4415 ,  A23L2/52 ,  A23L2/66 ,  A23L33/15 ,  A23L33/18 ,  A23L2/56 ,  A61K9/00 ,  A61K31/4164 ,  A23L33/175

摘要： Compositions and foods which contain (1) histidine and (2) vitamin B6 and/or carnosine are useful for treating, improving, and recovering from fatigue.

公开(公告)号：US20190227071A1

公开(公告)日：2019-07-25

申请号：US16373193

申请日：2019-04-02

申请人： AJINOMOTO CO., INC.

发明人： Hidehiro NAKAMURA ,  Natsumi NISHIKATA ,  Akira IMAIZUMI ,  Kazutaka SHIMBO ,  Naoko ARASHIDA ,  Rumi NISHIMOTO

IPC分类号： G01N33/574 ,  G01N33/49 ,  G01N33/487

摘要： An evaluating method includes an evaluating step of evaluating a state of pancreatic cancer for a subject to be evaluated using (i) a concentration value of at least one metabolite of N-Me-bABA, bABA, GABA, N6-Acetyl-L-Lys, 1-Me-His, aABA, Aminoadipic acid, bAiBA, Cadaverine, Ethylglycine, Homoarginine, Hypotaurine, Kinurenine, Putrescine, Serotonin, Spermidine, Spermine, ADMA, Homocitrulline, 3-Me-His, Hydroxyproline, Phosphoethanolamine, Acylcarnitine (13:1), and EPA in blood of the subject or (ii) a value of a formula calculated using the concentration value of the metabolite and the formula including an explanatory variable to be substituted with the concentration value of the metabolite.

公开(公告)号：US10322078B2

公开(公告)日：2019-06-18

申请号：US15374117

申请日：2016-12-09

申请人： AJINOMOTO CO., INC.

发明人： Mandai Yamada

IPC分类号： A61K8/67 ,  A61K8/34 ,  A61K8/37 ,  A61K8/39 ,  A61K8/44 ,  A61Q19/02 ,  A61K8/86 ,  A61K8/31 ,  A61K8/42 ,  A61K8/92

摘要： The present invention provides a cosmetic composition containing (A) 3-O-alkyl-L-ascorbic acid wherein the alkyl group has 8-12 carbon atoms or a salt thereof, (B) one or more kinds of nonionic surfactants selected from the group consisting of polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene alkyl ether and polyoxyethylene alkenyl ether, and having an HLB value of 12.5-17, and (C) one or more kinds of oil agents selected from the group consisting of amino acid ester, dimer acid ester, hydroxy acid ester, alkyl glyceryl ether and alkenyl glyceryl ether, which is superior in solubilization stability, preservation stability and sense of use, and free of time-dependent development of an odor which poses a problem when in use.

公开(公告)号：US10308969B2

公开(公告)日：2019-06-04

申请号：US14104139

申请日：2013-12-12

申请人： AJINOMOTO CO., INC.

发明人： Daisuke Ejima ,  Haruna Sato ,  Kouhei Tsumoto ,  Masayo Date

IPC分类号： C12P21/00 ,  C07K14/36 ,  C07K16/40 ,  C07K1/113 ,  C12P21/02

摘要： The present invention provides a method for producing a multimeric protein composed of a monomeric protein, wherein the monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.

公开(公告)号：US20190153416A1

公开(公告)日：2019-05-23

申请号：US16266542

申请日：2019-02-04

申请人： AJINOMOTO CO., INC.

发明人： Yusuke Hagiwara ,  Yasuhiro Mihara ,  Tomo Takagi ,  Satoshi Nakamura ,  Rie Yakushijin

IPC分类号： C12N9/24 ,  A23K10/14 ,  A23K20/189 ,  C12N1/20

摘要： Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.