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    Culture device for lactic acid bacteria
    63.
    发明授权
    Culture device for lactic acid bacteria 有权

    公开(公告)号：US10995356B2

    公开(公告)日：2021-05-04

    申请号：US15569579

    申请日：2016-04-26

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： Jason W. Bjork ,  Evan D. Brutinel ,  Adam J. Stanenas

    IPC: C12Q1/04 ,  C12M1/22 ,  C12M1/14 ,  C12M1/12 ,  C12M1/34 ,  C12M1/00 ,  C12N1/20 ,  C12P7/56

    Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.

    Device and Method for Dissecting and Analyzing Individual Cell Samples
    65.
    发明申请
    Device and Method for Dissecting and Analyzing Individual Cell Samples 审中-公开

    公开(公告)号：US20190106671A1

    公开(公告)日：2019-04-11

    申请号：US16086983

    申请日：2017-03-28

    Applicant: BIOSYNTAGMA, LLC

    Inventor： David Wayne Richardson ,  Dmitry Derkach ,  Colleen Ziegler ,  Steve Pemberton

    IPC: C12M1/00 ,  C12M1/14 ,  G01N1/28 ,  G01N15/14 ,  B01L3/00

    Abstract: A method for dissecting and collecting one or more cells from a tissue sample fixed to an inner surface of a microfluidic device is described. The tissue sample is in fluid communication with a channel having an inlet end and an outlet end defined by the microfluidic device. The method comprises flowing a first fluid through the channel with a fluid flow from the inlet end to the outlet end; powering a laser to direct laser energy into the channel to impinge upon the first fluid proximate a first region of the tissue sample and cause fluid cavitation to thereby ablate a first set of one or more cells from the tissue sample; and collecting the first set of one or more cells within a first sample container coupled to the outlet end.

    Method of increasing the gas-liquid interface in a fermentation process
    67.
    发明授权
    Method of increasing the gas-liquid interface in a fermentation process 有权
    在发酵过程中增加气 - 液界面的方法

    公开(公告)号：US08647862B2

    公开(公告)日：2014-02-11

    申请号：US12934649

    申请日：2009-03-30

    Applicant: Eino Elias Hakalehto

    Inventor： Eino Elias Hakalehto

    IPC: C12M1/14

    CPC classification number: C12M29/06 ,  C12M41/04 ,  C12M41/34

    Abstract: When using the equipment and method in accordance with this invention, it is possible to implement biotechnological production e.g. in biorefineries in such a way that it is possible to make use of the phenomena of the gas-liquid interface and interfaces between other phases by means of a moving process solution and gas led into it. This way, production and post-treatment methods can also be integrated, and desired acceleration of reactions and cost reductions can be achieved.

    Abstract translation: 当使用根据本发明的设备和方法时，可以实现生物技术生产。 在生物炼制中，可以通过移动的工艺溶液和引入其中的气体来利用气 - 液界面的现象和其它相之间的界面。 这样，也可以整合生产和后处理方法，并且可以实现期望的加速反应和降低成本。

    Bioreactor and method for producing microbial cellulose
    68.
    发明授权
    Bioreactor and method for producing microbial cellulose 有权
    生物反应器和生产微生物纤维素的方法

    公开(公告)号：US08551740B2

    公开(公告)日：2013-10-08

    申请号：US13332523

    申请日：2011-12-21

    Applicant: Hsu-Chou Hsiao ,  Ting-Sheng Lu ,  Han-Ken Chen ,  Jinn-Tsyy Lai ,  Fwu-Lin Lee ,  Chung-Liang Chu ,  Chii-Cherng Liao

    Inventor： Hsu-Chou Hsiao ,  Ting-Sheng Lu ,  Han-Ken Chen ,  Jinn-Tsyy Lai ,  Fwu-Lin Lee ,  Chung-Liang Chu ,  Chii-Cherng Liao

    IPC: C12P19/04 ,  C12M1/10 ,  C12M1/14

    CPC classification number: C12P19/04 ,  C12M27/06

    Abstract: A technique for producing microbial cellulose is provided, including: preparing a liquid medium for microbial cultivation in a container; horizontally rotating multiple hollow tubes that are fitted together or separated from one another, so that each of the hollow tubes is alternately partially immersed in the liquid medium and partially exposed above the horizontal surface of the liquid medium; wherein each of the hollow tubes has a rough outer surface and a smooth inner surface, so as to allow microorganisms to form microbial cellulose on the outer surface of each hollow tube, as well as forming sheets of microbial cellulose on the horizontal surface of the liquid medium not being disturbed by the hollow tubes, and removing the microbial cellulose from the outer surfaces of the hollow tubes in order to obtain tubular microbial cellulose. In addition, the sheets of microbial cellulose are also harvested from the liquid medium.

    Abstract translation: 提供了一种生产微生物纤维素的技术，包括：在容器中制备用于微生物培养的液体培养基; 水平旋转多个中空管，它们彼此装配在一起或分离，使得每个中空管交替地部分地浸没在液体介质中并部分地暴露在液体介质的水平表面之上; 其中每个中空管具有粗糙的外表面和平滑的内表面，以便允许微生物在每个中空管的外表面上形成微生物纤维素，以及在液体的水平表面上形成微生物纤维素片 介质不被中空管扰乱，并从中空管的外表面除去微生物纤维素，以获得管状微生物纤维素。 此外，微生物纤维素片也从液体培养基中收获。

    Porous sheet-form material for cell culture, and bioreactor and culturing method utilizing same
    69.
    发明授权
    Porous sheet-form material for cell culture, and bioreactor and culturing method utilizing same 失效
    用于细胞培养的多孔片状材料，以及利用其的生物反应器和培养方法

    公开(公告)号：US08435781B2

    公开(公告)日：2013-05-07

    申请号：US11574006

    申请日：2005-08-12

    Applicant: Makoto Kodama

    Inventor： Makoto Kodama

    IPC: C12M1/14 ,  C12M3/04 ,  C12M1/00 ,  C12M3/00

    CPC classification number: C12M23/04 ,  C12M23/34 ,  C12M25/02 ,  C12M29/04

    Abstract: The present invention provides a bioreactor having a system that can grow cells, tissue, etc. while maintaining or improving their function, and finally recover the cells as they are with good efficiency. The bioreactor has a porous sheet-form material disposed in its main body, the porous sheet-form material being formed from a nonwoven fabric, etc. having high cell affinity in order to retain cells. This porous sheet-form material has a thermosensitive polymer and a cell-adhesive substance incorporated thereinto, and the porous sheet-form material is not only cell-adhesive but also allows cells and tissue to be detached from the porous sheet-form material as they are by, for example, cooling from 37° C. to 25° C. Furthermore, in order to efficiently ensure the bioactivity or the survival of the cells, it is arranged so that circulation of a culture medium in a culturing space of the bioreactor is of a radial flow type.

    Abstract translation: 本发明提供一种生物反应器，其具有能够在保持或改善其功能的同时生长细胞，组织等的系统，并且最终以良好的效率原样回收细胞。 生物反应器具有设置在其主体中的多孔片状材料，多孔片状材料由具有高细胞亲和力的无纺布等形成，以保留细胞。 该多孔片状材料具有热敏性聚合物和结合在其中的细胞粘合剂物质，并且多孔片状材料不仅具有细胞粘附性，而且允许细胞和组织从多孔片状材料中分离，因为它们 通过例如从37℃冷却至25℃。此外，为了有效地确保细胞的生物活性或存活，布置成使生物反应器的培养空间中的培养基循环 是径向流型。

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