公开(公告)号：US20240043915A1

公开(公告)日：2024-02-08

申请号：US18546173

申请日：2022-02-11

Applicant: THE GENERAL HOSPITAL CORPORATION ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  THE BROAD INSTITUTE, INC.

Inventor： Evan Macosko ,  Nicolas Lapique ,  Michael Kim

IPC: C12Q1/6841

CPC classification number: C12Q1/6841

Abstract: The present disclosure relates to compositions and methods for detecting nucleic acid sequences (e.g., coding and non-coding RNAs; nuclear/genomic DNA; mtDNA; pathogen nucleic acids, etc.) in a tissue sample, specifically providing improved matrices and matrix-employing methods for performance of nucleic acid capture and amplification in a tissue sample in situ and/or in a manner that retains spatial location information for captured nucleic acids (including nucleic acid-associated macromolecules).

公开(公告)号：US11891593B2

公开(公告)日：2024-02-06

申请号：US17897560

申请日：2022-08-29

Applicant: The General Hospital Corporation

Inventor： Harald C. Ott

IPC: A01N1/00 ,  C12M3/00 ,  A01N1/02 ,  C12M1/00 ,  C12M1/36 ,  A61K35/42 ,  A61K35/36 ,  A61K35/44 ,  A61K35/28 ,  C12M1/12 ,  C12M1/34

CPC classification number: C12M21/08 ,  A01N1/0247 ,  A61K35/28 ,  A61K35/36 ,  A61K35/42 ,  A61K35/44 ,  C12M25/14 ,  C12M29/10 ,  C12M41/40 ,  C12M41/44 ,  C12M41/48

Abstract: Presented is an airway organ bioreactor apparatus, and methods of use thereof, as well as bioartificial airway organs produced using the methods, and methods of treating subjects using the bioartificial airway organs. The bioreactor comprises: an organ chamber: an ingres line connecting the organ chamber and a reservoir system and comprising an arterial line, a venous line and a tracheal line; an egress line connecting the chamber and the reservoir system, pumps in ingress and egress lines; a controller to control fluid exchange; a chamber pressure sensor connected to the organ chamber.

公开(公告)号：US11890348B2

公开(公告)日：2024-02-06

申请号：US15760772

申请日：2016-09-16

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： Mark C. Poznansky

IPC: A61K47/68 ,  A61K39/39 ,  A61K39/395 ,  A61P35/00 ,  A61K39/00 ,  C07K16/30 ,  C07K14/52 ,  A61K31/395 ,  A61K45/06

CPC classification number: A61K47/6803 ,  A61K31/395 ,  A61K39/39 ,  A61K39/395 ,  A61K45/06 ,  A61K47/6851 ,  A61P35/00 ,  C07K14/52 ,  C07K16/30 ,  A61K39/00

Abstract: The invention described herein relates to methods and compositions for treating cancer in a patient, or a tumor cell, by administering an effective amount of an antibody-anti-fugetactic agent complex.

公开(公告)号：US20240016933A1

公开(公告)日：2024-01-18

申请号：US18036047

申请日：2021-11-05

Applicant: The General Hospital Corporation

Inventor： James T. Kurnick ,  Ian Seymour Dunn ,  Matthew M. Lawler

IPC: A61K39/00 ,  C07K16/10 ,  C12N5/0783 ,  C07H15/12 ,  C07K16/28 ,  C07K16/30 ,  A61K35/17

CPC classification number: A61K39/4631 ,  C07K16/1018 ,  C12N5/0636 ,  C07H15/12 ,  C07K16/2803 ,  C07K16/3015 ,  C07K16/1027 ,  A61K35/17 ,  C07K16/2863 ,  A61K2239/13

Abstract: The present disclosure provides methods of anchoring active molecules on the surface of a cell, methods of anchoring at least two active molecules on the surface of a cell, and methods of enhancing an immune response to a target cell in a human.

公开(公告)号：US20240011049A1

公开(公告)日：2024-01-11

申请号：US18351674

申请日：2023-07-13

Applicant: The General Hospital Corporation ,  President and Fellows of Harvard College

Inventor： J. Keith Joung ,  Peter Cabeceiras

IPC: C12N15/86 ,  C07K14/005

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N2740/10023 ,  C12N2740/10042 ,  C12N2740/10052

Abstract: Human-derived virus-like particles (heVLPs), comprising a membrane comprising a phospholipid bilayer with one or more HERV-derived envelope proteins on the external side; one or more HERV-derived GAG proteins in the heVLP core, and a cargo molecule, e.g., a biomolecule and/or chemical cargo molecule, disposed in the core of the heVLP on the inside of the membrane, wherein the heVLP does not comprise a gag protein, except for gag proteins that are encoded in the human genome or gag proteins that are encoded by a consensus sequence that is derived from gag proteins found in the human genome, and methods of use thereof for delivery of the cargo molecule to cells.

公开(公告)号：US11866783B2

公开(公告)日：2024-01-09

申请号：US16318658

申请日：2017-07-21

Applicant: The General Hospital Corporation

Inventor： Thurman Wheeler ,  Xandra O. Breakefield ,  Leonora Balaj

IPC: C12Q1/68 ,  C07H21/04 ,  C12Q1/6883 ,  C12Q1/6865 ,  G01N33/50

CPC classification number: C12Q1/6883 ,  C12Q1/6865 ,  G01N33/50 ,  C12Q2600/106 ,  C12Q2600/156

Abstract: Described herein are methods for diagnosing and monitoring subjects with diseases associated with aberrant splicing, based upon detecting properly spliced isoforms and mis-spliced isoforms in a urine sample from the subject.

公开(公告)号：US11866736B2

公开(公告)日：2024-01-09

申请号：US15749218

申请日：2016-08-01

Applicant: The General Hospital Corporation

Inventor： Vamsi Mootha ,  Denis Titov ,  Valentin Cracan ,  Zenon Grabarek

IPC: C12N9/02 ,  C12N5/10 ,  C07K14/335 ,  C07K14/195 ,  C12N5/071 ,  C12N15/52 ,  C12N15/85 ,  A61K47/66 ,  A61P25/16 ,  A61K9/127 ,  A61K38/16 ,  A61K38/17 ,  A61K47/18 ,  A61K47/42 ,  C12N15/113 ,  C12N15/62

CPC classification number: C12N9/0036 ,  C07K14/195 ,  C07K14/335 ,  C12N5/0602 ,  C12N5/10 ,  C12N15/52 ,  C12N15/85 ,  C12Y106/03001 ,  A61K9/127 ,  A61K38/162 ,  A61K38/1709 ,  A61K38/1774 ,  A61K47/18 ,  A61K47/42 ,  A61K47/66 ,  A61P25/16 ,  C07K2319/07 ,  C07K2319/43 ,  C12N15/113 ,  C12N15/62

Abstract: The invention encompasses water-forming NADH and NADPH oxidases and the use of these enzymes to treat mammalian diseases or conditions associated with an elevated NADH/NAD+ ratio or NADPH/NADP+ ratio. Such pathologies include disorders caused by one or more defects in the mitochondrial respiratory chain, glucose metabolism disorders, cancers associated with reductive stress, and aging. The invention also provides a research tool for investigating the effect of exogenous water-forming NADH or NADPH oxidases on the metabolism of a mammalian cell, such as a human cell, and for elucidating the role of respiratory chain proteins in mitochondrial disorders.

公开(公告)号：US20240002536A1

公开(公告)日：2024-01-04

申请号：US18035982

申请日：2021-11-05

Applicant: The General Hospital Corporation

Inventor： James T. Kurnick ,  Ian Seymour Dunn ,  Matthew M. Lawler

IPC: C07K16/44 ,  G01N33/53

CPC classification number: C07K16/44 ,  G01N33/5308 ,  C07K2317/92 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2317/622

Abstract: The present disclosure is directed, at least in part, to click-product binding molecules for recognition of templated assembly products defined by bioorthogonal click groups. Such molecules can be used for therapeutic and diagnostic purposes, including kits for the same.

公开(公告)号：US20230414450A1

公开(公告)日：2023-12-28

申请号：US18251878

申请日：2021-11-05

Applicant: The General Hospital Corporation

Inventor： Ryan W. Carroll ,  Kimberly Whalen ,  Matthew Beatty

IPC: A61J1/20 ,  A61M39/10

CPC classification number: A61J1/20 ,  A61M39/10 ,  A61M2039/1088 ,  A61M2039/1083 ,  A61M2039/009

Abstract: Systems and methods for shielded fluid transfer are provided. A fluid transfer shield is provided to facilitate transferring fluid from a first container to a secondary container. The fluid transfer shield includes a shield having an exterior and defining an interior space, a first port extending from the exterior of the shield, the first port configured to be fluidly coupled to the first container, and a second port extending opposite the first port and into the interior space of the shield so that the shield laterally surrounds the second port, the second port configured to be fluidly coupled to the secondary container. A passageway is provided that extends between the first port and the second port to fluidly couple the first container and the secondary container when the first container is fluidly coupled to the first port and the secondary container is fluidly coupled to the second port.

公开(公告)号：US20230407397A1

公开(公告)日：2023-12-21

申请号：US18035269

申请日：2021-11-04

Applicant: Massachusetts Institute of Technology ,  The General Hospital Corporation

Inventor： Nir Hacohen ,  Ang Cui

IPC: C12Q1/6881 ,  C12Q1/6809 ,  A61K45/06

CPC classification number: C12Q1/6881 ,  C12Q1/6809 ,  A61K45/06 ,  C12Q2600/136

Abstract: The present disclosure provides methods for determining signatures of immune responses to immunomodulating agents in cells and in tissues. Also provided herein are computational methods for deconvoluting gene expression profiling data. Further provided herein are methods for treating a disease or disorder (e.g, proliferative diseases such as cancer, autoimmune diseases such as rheumatoid arthritis and psoriasis, allergies, etc.) in a subject comprising administering one or more immunomodulating agents to drive an immune response, in combination with a treatment for the disease or disorder (e.g, an anti-cancer therapy, an anti-viral therapy, a vaccination, etc.).