公开(公告)号：US20060199242A1

公开(公告)日：2006-09-07

申请号：US11366784

申请日：2006-03-01

Applicant: Ching-Ying Cheung ,  Diane Gray ,  Stephen Yue

Inventor： Ching-Ying Cheung ,  Diane Gray ,  Stephen Yue

IPC: C12Q1/18 ,  G01N33/18 ,  C07D417/02

CPC classification number: G01N33/1806 ,  C07D211/62 ,  C07D295/112 ,  C07D401/14 ,  C07D417/06 ,  C07D417/14 ,  C09B23/04 ,  C12Q1/04 ,  C12Q1/18 ,  G01N21/643 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6432

Abstract: Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.

公开(公告)号：US20050191643A1

公开(公告)日：2005-09-01

申请号：US10916822

申请日：2004-08-11

Applicant: Rosaria Haugland ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Rosaria Haugland ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07D209/12 ,  C07D209/24 ,  C07D401/04 ,  C07D471/02 ,  C07D491/02 ,  C07D498/02 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C07D209/12 ,  C07D209/14 ,  C07D209/24 ,  C07D401/04 ,  C09B23/12

Abstract: The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.

Abstract translation: 本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。 本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。 能量转移对用于检测样品中感兴趣的分析物。

公开(公告)号：US06466008B1

公开(公告)日：2002-10-15

申请号：US09680577

申请日：2000-10-06

Applicant: Patrick Ying-Cheung Fung ,  Carl Lewis Wuebker

Inventor： Patrick Ying-Cheung Fung ,  Carl Lewis Wuebker

IPC: G06F1750

CPC classification number: H05K3/0005 ,  G06F17/5077 ,  H05K1/0237 ,  H05K1/14 ,  H05K2201/044 ,  H05K2201/09236

Abstract: Method for obtaining a difference in lengths among a plurality of signal traces formed on two different surfaces, includes dividing a propagation delay of a first surface into a propagation delay of a second surface to obtain a correction factor. The segment of the signal traces formed on the second surface is adjusted by multiplying the correction factor to this segment. Then, the adjusted segment is summed with the segment of the signal traces formed on the first surface to obtain a total length. The total length of each of the signal traces is subtracted from the longest total length of the signal traces.

Abstract translation: 用于获得形成在两个不同表面上的多个信号迹线之间的长度差的方法，包括将第一表面的传播延迟划分为第二表面的传播延迟以获得校正因子。 通过将校正因数乘以该段来调整形成在第二表面上的信号迹线的段。 然后，将调整后的段与形成在第一表面上的信号迹线的段相加，以获得总长度。 每个信号迹线的总长度从信号迹线的最长总长度中减去。

公开(公告)号：US09225775B2

公开(公告)日：2015-12-29

申请号：US12272618

申请日：2008-11-17

Applicant: Ivy Pei-Shan Hsu ,  David Chun Ying Cheung ,  Rajkumar Ramniranjan Jalan

Inventor： Ivy Pei-Shan Hsu ,  David Chun Ying Cheung ,  Rajkumar Ramniranjan Jalan

IPC: G06F15/16 ,  G06F15/173 ,  H04L29/08

CPC classification number: H04L67/1002 ,  H04L67/1008 ,  H04L67/1012 ,  H04L67/1021

Abstract: A global server load balancing (GSLB) switch serves as a proxy to an authoritative DNS communicates with numerous site switches which are coupled to host servers serving specific applications. The GSLB switch receives from site switches operational information regarding host servers within the site switches neighborhood. When a client program requests a resolution of a host name, the GSLB switch, acting as a proxy of an authoritative DNS, returns one or more ordered IP addresses for the host name. The IP addresses are ordered using metrics that include the information collected from the site switches. In one instance, the GSLB switch places the address that is deemed “best” at the top of the list.

Abstract translation: 全局服务器负载平衡（GSLB）交换机用作授权DNS的代理，与许多站点交换机进行通信，这些站点交换机与服务于特定应用程序的主机服务器相连接。 GSLB交换机从站点交换机接收关于站点交换机附近的主机服务器的操作信息。 当客户端程序请求解析主机名时，作为权威DNS的代理的GSLB交换机返回主机名的一个或多个有序IP地址。 IP地址使用包含从站点交换机收集的信息的度量来排序。 在一个实例中，GSLB开关将地址视为“最佳”位于列表的顶部。

公开(公告)号：US09193746B2

公开(公告)日：2015-11-24

申请号：US11952867

申请日：2007-12-07

Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Jie Yang

Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Jie Yang

IPC: G01N21/64 ,  C07F15/00 ,  C09B57/10 ,  G01N33/68

CPC classification number: C07F15/0053 ,  C09B57/10 ,  G01N33/6839 ,  G01N2458/40

Abstract: Luminescent metal complexes, methods of producing and/or designing same, methods of using same, and associated technology, are disclosed herein. A luminescent metal complex may be useful for a variety of applications, such as staining, detection, and/or identification, for example, of substances, such as poly(amino acids), for example. Further by way of example, a luminescent metal complex may be useful for staining, detecting, and/or identifying poly(amino acids) that are associated with any of various environments, such as a gel or a gel matrix, such as any associated with SDS-PAGE, for example, a surface environment, such as any associated with western blot, for example, and/or the like. Compositions, solutions, and kits comprising a luminescent metal complex are also disclosed herein.

Abstract translation: 本文公开了发光金属络合物，其制备和/或设计方法，使用方法及相关技术。 发光金属络合物可用于各种应用，例如染色，检测和/或鉴定，例如物质，例如聚（氨基酸）。 进一步作为示例，发光金属络合物可用于染色，检测和/或鉴定与各种环境中的任何环境相关的聚（氨基酸），例如凝胶或凝胶基质，例如任何与 例如，SDS-PAGE，例如表面环境，例如与western印迹相关的任何例如和/或类似物。 本文还公开了包含发光金属络合物的组合物，溶液和试剂盒。

公开(公告)号：US20120288871A1

公开(公告)日：2012-11-15

申请号：US13556643

申请日：2012-07-24

Applicant: Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07D403/06 ,  C07D471/04 ,  G01N1/30 ,  C07K14/00 ,  C07K16/18 ,  C07H21/02 ,  G01N33/53 ,  C08B37/02 ,  C12N1/20 ,  C07H19/02 ,  C07H21/04 ,  C07D403/14 ,  C07K14/36

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了结合通过反应性基团或共轭物质在3位被取代的吲哚环部分的化学活性碳菁染料及其用途。 通过该位置的共轭导致光谱性质均匀地优于光谱相似的染料的缀合物，其中附着在不同的位置。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US20120252063A1

公开(公告)日：2012-10-04

申请号：US13439821

申请日：2012-04-04

Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Lori Michelle Roberts

Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Lori Michelle Roberts

IPC: C09B1/516 ,  G01N1/30 ,  G01N21/64

CPC classification number: C09B1/516 ,  A61K49/0052 ,  C09B1/501 ,  C09B1/51 ,  C09B1/5155 ,  G01N1/30 ,  G01N21/6428

Abstract: The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.

Abstract translation: 本发明公开了可用作细胞污渍的新的取代的蒽醌染料。 在本发明的某些方面，核染料可用于染色死细胞或固定细胞的细胞核。 本发明的另一方面涉及包含酶底物部分的取代的蒽醌染料，其可由酶转化或切割，使得底物部分的转化或切割导致染料的官能度或光谱性质的可检测变化。

公开(公告)号：US08241852B2

公开(公告)日：2012-08-14

申请号：US13017242

申请日：2011-01-31

Applicant: Stephen Yue ,  Ching-Ying Cheung

Inventor： Stephen Yue ,  Ching-Ying Cheung

IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/02

CPC classification number: C12Q1/6816 ,  Y10S436/80 ,  C12Q2563/173

Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.

Abstract translation: 本发明的实施方案提供了用于检测样品中核酸的方法和核酸报告分子。 核酸报告分子包含两个不对称的花青单体部分，其可以相同或不同，其通过包含至少一个芳族，杂芳族，环状或杂环部分的连接体共价连接，该部分包含3-20个非氢原子，所述非氢原子选自 由O，N，S，P和C组成的组。接头可以是刚性的，相对柔性的或其某种程度的。 不对称花青单体部分包含取代或未取代的苯并唑鎓部分和通过单甲基，三甲基或五甲氧基的次甲基桥连接的取代或未取代的吡啶鎓或喹啉鎓部分。 连接体通过连接到单体部分的吡啶鎓或喹啉鎓部分而形成花青二聚体化合物。 本发明的核酸报告分子可用于形成核酸 - 报道分子复合物并检测核酸。 特别地，具有刚性连接体和具有单甲基桥的单体部分的本发明的核酸报告分子在DNA存在下可用于检测RNA。

公开(公告)号：US20110124085A1

公开(公告)日：2011-05-26

申请号：US13019397

申请日：2011-02-02

Applicant: Stephen YUE ,  Ching-Ying Cheung

Inventor： Stephen YUE ,  Ching-Ying Cheung

IPC: C07H21/02 ,  C07H21/04 ,  C07K9/00 ,  C07K14/00 ,  C12N5/00 ,  C12N7/00 ,  C07K16/00

CPC classification number: C12Q1/6816 ,  Y10S436/80 ,  C12Q2563/173

Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.

公开(公告)号：US20110105362A1

公开(公告)日：2011-05-05

申请号：US13005002

申请日：2011-01-12

Applicant: Rosaria P. Haugland ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Rosaria P. Haugland ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C40B40/04 ,  C12N9/96 ,  C12N7/00 ,  C12N1/00 ,  C07K2/00 ,  C07K14/00 ,  C07K16/00 ,  C07H21/00 ,  C07H19/00 ,  C07D209/56 ,  C07D209/18

CPC classification number: C12Q1/6876 ,  C07D209/12 ,  C07D209/14 ,  C07D209/24 ,  C07D401/04 ,  C09B23/12

Abstract: The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.

Abstract translation: 本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。 本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。 能量转移对用于检测样品中感兴趣的分析物。