Surface modification of silk fibroin matrices with poly(ethylene glycol) useful as anti-adhesion barriers and anti-thrombotic materials
    71.
    发明授权
    Surface modification of silk fibroin matrices with poly(ethylene glycol) useful as anti-adhesion barriers and anti-thrombotic materials 有权
    用聚乙二醇作为抗粘连屏障和抗血栓形成材料的丝素蛋白基质的表面改性

    公开(公告)号:US09427499B2

    公开(公告)日:2016-08-30

    申请号:US13129738

    申请日:2009-11-17

    摘要: The present invention provides compositions and methods for the production of silk fibroin matrices surface-PEGylated on one or more surfaces. Such surface-PEGylated silk fibroin matrices can be used in biomedical applications, such as anti-adhesive and anti-thrombosis materials. Silk matrices may be surface-PEGylated, for example, by a reaction with a functional group-activated PEG. Controlling the degree of PEGylation on surface of silk fibroin matrix can regulate both the degradation rate of the silk matrix, and the differentiated adhesion of cells or differentiated adsorption of proteins on the surface of the silk matrix. The present invention also provides for silk fibroin matrices having one or more surfaces possessing differentiated adhesion properties, which allows for tissue integration on the adherent side and inhibition of tissue adhesion to the opposing tissues or organs. Embedding active agents in silk fibroin matrices provides more benefits, such as promoting tissue ingrowth on the adherent side of the matrix.

    摘要翻译: 本发明提供了用于生产在一个或多个表面上表面聚乙二醇化的丝素蛋白基质的组合物和方法。 这种表面聚乙二醇化的丝素蛋白基质可用于生物医学应用,例如抗粘连和抗血栓形成的材料。 丝质基质可以被表面聚乙二醇化,例如通过与官能团活化的PEG的反应。 控制丝素蛋白基质表面聚乙二醇化程度可以调节蚕丝基质的降解速率,分化细胞粘附或蛋白质在丝质基质表面的分化吸附。 本发明还提供具有一个或多个具有分化的粘附性质的表面的丝素蛋白基质,其允许组合物在粘附层上的整合并抑制组织对相对组织或器官的粘附。 在丝素蛋白基质中包埋活性剂提供了更多的益处,例如促进组织向上生长在基质的粘着面上。

    All-protein implantable, resorbable reflectors
    72.
    发明授权
    All-protein implantable, resorbable reflectors 有权
    全蛋白可植入,可吸收反射体

    公开(公告)号:US09016875B2

    公开(公告)日:2015-04-28

    申请号:US13386388

    申请日:2010-07-20

    IPC分类号: G02B5/124 A61B5/00 A61L27/22

    摘要: The invention provides for compositions and process for fabricating an optical reflector constructed from biocompatible and bioresorbable silk fibroin proteins. For example, the silk retroreflectors may be built based on millimeter size microprism arrays to rotate the image plane of imaged cortical layers, thus enhancing the amount of photons that are detectable in the reflected direction when inserted in a sample to be analyzed, and ultimately increasing in contrast ratio in multiphoton microscopy. Such device can be used as a label-free, biocompatible, bioresorbable, implantable device for various applications ranging from medical imaging/diagnostics, drug/therapeutic delivery, to food chain safety and environmental monitoring.

    摘要翻译: 本发明提供了用于制造由生物相容性和生物可再吸收的丝素蛋白构成的光反射体的组合物和方法。 例如,丝绸后向反射器可以基于毫米尺寸的微棱镜阵列构建,以旋转成像的皮质层的图像平面,从而增加当插入待分析的样品中时在反射方向上可检测的光子的量,并且最终增加 在多光子显微镜中的对比度。 这种装置可用作用于各种应用的无标签,生物相容性,生物可再吸收的可植入装置,从医学成像/诊断,药物/治疗递送,食品链安全和环境监测。

    Silk-based drug delivery system
    74.
    发明授权
    Silk-based drug delivery system 有权
    丝绸药物输送系统

    公开(公告)号:US08530625B2

    公开(公告)日:2013-09-10

    申请号:US13443264

    申请日:2012-04-10

    IPC分类号: A61K38/17 A61K9/22

    摘要: The present invention provides for novel sustained release silk-based delivery systems. The invention further provides methods for producing such formulations. In general, a silk fibroin solution is combined with a therapeutic agent to form a silk fibroin article. The article is then treated in such a way as to alter its conformation. The change in conformation increases its crytallinity or liquid crystallinity, thus controlling the release of a therapeutic agent from the formulation. This can be accomplished as single material carriers or in a layer-by-layer fashion to load different therapeutic agents or different concentrations of these agents in each layer.

    摘要翻译: 本发明提供了新颖的持续释放的基于丝的递送系统。 本发明还提供了生产这种制剂的方法。 通常,将丝素蛋白溶液与治疗剂组合以形成丝素蛋白制品。 然后对该文章进行处理,以改变其构象。 构象的变化增加其胆固醇或液体结晶度,从而控制治疗剂从制剂中的释放。 这可以作为单一材料载体或以逐层方式实现,以在每个层中加载不同的治疗剂或不同浓度的这些试剂。

    Removable tailgate gap cover
    75.
    发明授权
    Removable tailgate gap cover 有权
    可拆卸后挡板间隙盖

    公开(公告)号:US08459716B2

    公开(公告)日:2013-06-11

    申请号:US13328375

    申请日:2011-12-16

    申请人: David L. Kaplan

    发明人: David L. Kaplan

    IPC分类号: B62D33/03

    CPC分类号: B62D33/0273

    摘要: A removable tailgate gap cover for preventing ingress of debris in a tailgate gap. The gap cover comprises an elongated cover plate having a top surface, a bottom surface, two longitudinal edges and an elongated support bar having an upper end and a lower end, wherein the upper end is fixedly attached to and substantially centrally aligned with the lengthwise direction of the bottom surface. The elongated support bar comprises a magnetic attaching means disposed along the lower end of the elongated support bar for attaching the gap cover to the floor of the end portion of the truck bed.

    摘要翻译: 可拆卸的后挡板间隙盖,用于防止尾门间隙中的碎屑进入。 间隙盖包括细长的盖板,其具有顶表面,底表面,两个纵向边缘和具有上端和下端的细长支撑杆,其中上端固定地附接到长度方向 的底面。 细长的支撑杆包括沿着细长支撑杆的下端设置的磁性附接装置,用于将间隙盖附接到卡车车厢底部的底部。

    BIOENGINEERED SILK PROTEIN-BASED NUCLEIC ACID DELIVERY SYSTEMS
    76.
    发明申请
    BIOENGINEERED SILK PROTEIN-BASED NUCLEIC ACID DELIVERY SYSTEMS 审中-公开
    生物基质蛋白基于核酸的交付系统

    公开(公告)号:US20120171770A1

    公开(公告)日:2012-07-05

    申请号:US13381105

    申请日:2010-07-09

    摘要: Nucleic acid transfer is achieved using a silk-based delivery system which releases nucleic acids from silk-based complexes. The silk-based complexes, which are composed, for example, of plasmid DNA (pDNA) and recombinant silk containing polycation and specific polypeptides sequences, can show high biocompatibility, high delivery efficiency, cell selectivity and controlled release of nucleic acid for nucleic acid transfection.

    摘要翻译: 使用从丝基络合物释放核酸的基于丝的递送系统实现核酸转移。 例如由质粒DNA(pDNA)和含重组丝聚阳离子和特异性多肽序列组成的丝基复合物可显示出高的生物相容性,高递送效率,细胞选择性和用于核酸转染的核酸的控制释放 。

    POLYSACCHARIDE COMPOSITION AND METHODS OF ISOLATION OF THE EMULSION STABILIZING CATIONIC POLYELECTROLYTIC POLYSACCHARIDE
    78.
    发明申请
    POLYSACCHARIDE COMPOSITION AND METHODS OF ISOLATION OF THE EMULSION STABILIZING CATIONIC POLYELECTROLYTIC POLYSACCHARIDE 失效
    多糖组合物和乳液稳定阳离子聚电解多糖的分离方法

    公开(公告)号:US20110206772A1

    公开(公告)日:2011-08-25

    申请号:US12993711

    申请日:2009-05-28

    摘要: The present invention relates to purification and use of a novel emulsion stabilizing polysaccharide. In particular, a polyelectrolyte exopolysaccharide with high molecular weight comprising a high molecular weight polymer with a tri-saccharide repeating unit is disclosed. In one aspect of the invention, methods are directed to isolating and purifying a high molecular weight exopolysaccharide (EPS) from a cell supernatant. In another aspect, methods are disclosed for isolating a lipopolysaccharide (LPS) and a high molecular weight Acinetobacter polyelectrolyte exopolysaccharide (APE) from Acinetobacter bacteria. Compositions are also directed to lipid nanoparticles comprising a therapeutic agent encapsulated by a high molecular weight polysaccharide and nanoparticles comprising a therapeutic agent bound to a cationic polysaccharide cross-linked with a polyanion.

    摘要翻译: 本发明涉及新型乳化稳定多糖的纯化和应用。 特别地,公开了具有高分子量的聚电解质外多糖,其包含具有三糖重复单元的高分子量聚合物。 在本发明的一个方面,方法涉及从细胞上清液中分离和纯化高分子量外多糖(EPS)。 在另一方面,公开了从不动细菌分离脂多糖(LPS)和高分子量不动杆菌聚电解质外多糖(APE)的方法。 组合物还涉及包含由高分子量多糖包封的治疗剂和包含与聚阴离子交联的阳离子多糖结合的治疗剂的纳米颗粒的脂质纳米颗粒。