公开(公告)号：US10006055B2

公开(公告)日：2018-06-26

申请号：US14869872

申请日：2015-09-29

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/04 ,  C12N15/52 ,  C12P5/02 ,  C12N15/70 ,  C12P7/16

CPC classification number: C12P7/04 ,  C12N15/52 ,  C12N15/70 ,  C12P5/026 ,  C12P7/16

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.

公开(公告)号：US09982281B2

公开(公告)日：2018-05-29

申请号：US14692630

申请日：2015-04-21

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/18 ,  C12N15/52

CPC classification number: C12P7/18 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

公开(公告)号：US09909150B2

公开(公告)日：2018-03-06

申请号：US14071318

申请日：2013-11-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12N1/20 ,  C12N1/16 ,  C12N1/14 ,  C12P7/20 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/52

CPC classification number: C12P7/24 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/20 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

公开(公告)号：US09885064B2

公开(公告)日：2018-02-06

申请号：US14185709

申请日：2014-02-20

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

IPC: C12N1/20 ,  C12N1/12 ,  C12P7/62 ,  C12P7/04 ,  C07H21/04 ,  C12P7/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12P19/32 ,  C12N15/70

CPC classification number: C12P7/18 ,  C12N9/0008 ,  C12N9/0036 ,  C12N9/1007 ,  C12N9/93 ,  C12N15/70 ,  C12P19/32 ,  Y02E50/343

Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

公开(公告)号：US09732361B2

公开(公告)日：2017-08-15

申请号：US14059131

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Jun Sun ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C08F36/00 ,  C12P5/02 ,  C12N1/20 ,  C12N1/00 ,  C07C9/00 ,  C12N15/52 ,  C07C11/16 ,  C08F136/06

CPC classification number: C12P5/026 ,  C07C11/16 ,  C08F136/06 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

公开(公告)号：US20160053286A1

公开(公告)日：2016-02-25

申请号：US14673600

申请日：2015-03-30

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/18 ,  C07C29/80 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

Abstract translation: 非天然存在的微生物生物体包括具有1,3-丁二醇（1,3-BDO）途径的微生物生物，其具有至少一种编码1,3-BDO途径酶的外源核酸，所述外源核酸以足够的量表达，以产生1 ，3-BDO。 该途径包括选自2-氨基-4-酮戊酸酯（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸转氨酶，2-氨基-4-羟基戊酸酯氧化还原酶（脱氨基），2- 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（还原酮），3-氧代丁醛还原酶 ），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮氨基转移酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶 乙酰乙酰辅酶A还原酶（CoA依赖性醛形成），乙酰乙酰辅酶A还原酶（CoA依赖性醇形成），乙酰乙酰辅酶A还原酶（酮），乙酰乙酰辅酶A还原酶 还原），3-羟基丁酰辅酶A还原酶（醛形成），3-羟基 Ryl-CoA还原酶（醇形成），4-羟基丁酰辅酶A脱水酶和巴豆酸酶。 一种生产1,3-BDO的方法包括在条件和足够的时间内培养这些微生物生产1,3-BDO。

公开(公告)号：US20130316426A1

公开(公告)日：2013-11-28

申请号：US13887140

申请日：2013-05-03

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

IPC: C07C51/38 ,  C07C67/32

CPC classification number: C07C51/353 ,  C07C51/38 ,  C07C57/04 ,  C07C67/08 ,  C07C67/32 ,  C07C67/333 ,  C12P7/40 ,  C12P7/46 ,  C12P7/62 ,  Y02P20/125 ,  C07C69/60 ,  C07C69/54

Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

Abstract translation: 本发明提供了制备丙烯酸的方法。 该方法包括在交叉复分解转化催化剂存在下使富马酸与足够量的乙烯接触，以产生约2摩尔丙烯酸/摩尔富马酸。 还提供了丙烯酸酯。 该方法包括在交叉复分解转化催化剂存在下使富马酸二酯与足够量的乙烯接触，以产生每摩尔富马酸二酯约2摩尔丙烯酸酯。 提供了用于生产丙烯酸或丙烯酸酯的方法的综合方法，其将富马酸的生物生产与易位转化相结合。 还提供丙烯酸和丙烯酸酯生产。

公开(公告)号：US11834690B2

公开(公告)日：2023-12-05

申请号：US17527902

申请日：2021-11-16

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P13/02 ,  C12P17/12

CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US11629363B2

公开(公告)日：2023-04-18

申请号：US15873798

申请日：2018-01-17

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P7/18 ,  C12P7/20 ,  C12P7/24 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/52 ,  C12P7/04

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

公开(公告)号：US11085015B2

公开(公告)日：2021-08-10

申请号：US15585078

申请日：2017-05-02

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya ,  Anthony P. Burgard ,  Stephen J. Van Dien ,  Robin E. Osterhout ,  Mark J. Burk ,  John D. Trawick ,  Michael P. Kuchinskas ,  Brian Steer

IPC: C12P7/42 ,  C12P7/18 ,  C12N1/20 ,  C12N1/14 ,  C12N1/16 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C07C31/20 ,  C07C59/01 ,  C08G18/28 ,  C08G18/32 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.