公开(公告)号：US20090258402A1

公开(公告)日：2009-10-15

申请号：US12034101

申请日：2008-02-20

申请人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/20

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A method for producing L-glutamic acid by culturing a coryneform bacterium which has L-glutamic acid producing ability and which has been modified so that expression of the fasR gene is enhanced in a medium to produce and accumulate L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium or cells.

摘要翻译： 通过培养具有L-谷氨酸生产能力的棒状细菌生产L-谷氨酸的方法，其已被修饰以使培养基中的fasR基因的表达增强以产生和积累L-谷氨酸，或者 细胞，并从培养基或细胞中收集L-谷氨酸。

公开(公告)号：US07344874B2

公开(公告)日：2008-03-18

申请号：US11070085

申请日：2005-03-03

申请人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

发明人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

IPC分类号： C12N1/20 ,  C12P13/14

CPC分类号： C12P13/14 ,  C07K14/34

摘要： L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

摘要翻译： 通过培养在培养基中培养L-谷氨酸输出基因，yhfK基因的表达增强或过表达的微生物来产生L-谷氨酸以产生和导致培养基中L-谷氨酸的积累，以及 从培养基中收集L-谷氨酸。

公开(公告)号：US07329523B2

公开(公告)日：2008-02-12

申请号：US11245030

申请日：2005-10-07

申请人： Mikiko Suga ,  Yoko Asakura ,  Masakazu Sugimoto ,  Hisao Ito

发明人： Mikiko Suga ,  Yoko Asakura ,  Masakazu Sugimoto ,  Hisao Ito

IPC分类号： C12N9/14 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/68 ,  C12Q1/42 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12Y301/03003 ,  C12N9/16 ,  C12P13/06

摘要： The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.

摘要翻译： 本发明提供了编码下列（A）或（B）中定义的蛋白质的DNA，其通过克隆将大肠杆菌的SerB缺陷复杂化的DNA片段作为DNA片段的开放阅读框，从黄色短杆菌染色体DNA文库获得 。 （A）包含序列表中SEQ ID：2的氨基酸序列的蛋白质; 或（B）包含序列表中SEQ ID NO：2的氨基酸序列中的一个或几个氨基酸的取代，缺失，插入，添加或倒位，并具有磷酸丝氨酸磷酸酶活性的氨基酸序列的蛋白质。

公开(公告)号：US20070292001A1

公开(公告)日：2007-12-20

申请号：US11889999

申请日：2007-08-17

申请人： Kozo Baba ,  Hisao Ito ,  Akiyoshi Tafuku ,  Masatoshi Tohno ,  Taku Katagiri

发明人： Kozo Baba ,  Hisao Ito ,  Akiyoshi Tafuku ,  Masatoshi Tohno ,  Taku Katagiri

IPC分类号： G06K9/00

CPC分类号： G06K9/00248 ,  G06T7/74 ,  G06T2207/30201

摘要： For example, a plurality of elliptical candidate areas that become candidate areas of detecting objects are set in an image, characteristic values such as average values of luminance of the pixels inside and outside the set candidate areas are compared, and a candidate area whose difference of the characteristic values is determined to be the maximum is detected as the area of the detecting object. In addition, central moment of the detecting object is calculated, and the inclination of the detecting object in the image is detected based on the calculated central moment. Further, two specific points such as eyes of the driver included in the detecting object are detected, and the inclination of the detecting object in the image determined by the detected two points is detected.

摘要翻译： 例如，将成为检测对象的候补区域的多个椭圆候选区域设定为图像，比较设定候选区域内外的像素的亮度的平均值等特征值， 将特征值确定为最大值被检测为检测对象的区域。 此外，计算检测对象的中心矩，并且基于计算出的中心时刻来检测图像中的检测对象的倾斜度。 此外，检测包括在检测对象中的驱动器的眼睛的两个特定点，并且检测由检测到的两个点确定的图像中的检测对象的倾斜度。

公开(公告)号：US20070172932A1

公开(公告)日：2007-07-26

申请号：US11682370

申请日：2007-03-06

申请人： Seiko Hirano ,  Mikiko Yamaguchi ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Mikiko Yamaguchi ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12P13/14 ,  C12N9/0012

摘要： A coryneform bacterium which has an L-glutamic acid-producing ability and grows at least at the same growth rate as a non-mutated strain or a wild-type strain and has intracellular α-ketoglutarate dehydrogenase activity which is less than half that of the non-mutated or wild-type strain, and is obtained by introducing a mutation into a coding region or an expression control region of the chromosomal odhA gene encoding the E1o subunit of the α-ketoglutarate dehydrogenase complex.

摘要翻译： 具有L-谷氨酸生产能力的棒状杆菌型细菌至少以与非突变菌株或野生型菌株相同的生长速率生长，并且具有小于所述野生型菌株的一半的细胞内α-酮戊二酸脱氢酶活性 非突变或野生型菌株，并且通过将突变引入编码α-酮戊二酸脱氢酶复合物的E1o亚基的染色体odhA基因的编码区域或表达控制区而获得。

公开(公告)号：US07209574B2

公开(公告)日：2007-04-24

申请号：US10763209

申请日：2004-01-26

申请人： Akiyoshi Tafuku ,  Kozo Baba ,  Hisao Ito ,  Tomonobu Takashima ,  Tadao Omi

发明人： Akiyoshi Tafuku ,  Kozo Baba ,  Hisao Ito ,  Tomonobu Takashima ,  Tadao Omi

IPC分类号： G06K9/00

CPC分类号： G06K9/00604 ,  G06T7/215 ,  G06T7/248 ,  G06T2207/10016 ,  G06T2207/30201 ,  G06T2207/30248 ,  G06T2207/30268

摘要： Obtaining an image frame picked up by a camera 2 through a cable C, a CPU 10 of a computer 1 identifies an eye area in the image frame and stores a template indicating the identified area as a tracking template. Moreover, the CPU 10 identifies an eye area in an image frame to be obtained subsequently on the basis of the tracking template, and calculates a correlation value between the template of the identified area and the tracking template. Furthermore, when the calculated correlation value is larger than a threshold stored in an HD 11 in advance, the CPU 10 stores the template indicating the identified area in the RAM 12 as a tracking template, so as to update an eye area in a series of two image frames in chronological order.

摘要翻译： 通过电缆C获取由相机2拾取的图像帧，计算机1的CPU 10识别图像帧中的眼睛区域，并将指示识别的区域的模板存储为跟踪模板。 此外，CPU 10基于跟踪模板识别随后将要获得的图像帧中的眼睛区域，并且计算识别区域的模板和跟踪模板之间的相关值。 此外，当计算出的相关值大于预先存储在HD11中的阈值时，CPU 10将指示识别区域的模板存储在RAM 12中作为跟踪模板，以便更新一系列 两幅图像帧按时间顺序排列。

公开(公告)号：US07208296B2

公开(公告)日：2007-04-24

申请号：US11150265

申请日：2005-06-13

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12P13/14 ,  C12N15/00 ,  C12N1/20 ,  C12N1/36 ,  C12N15/63

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 提供了能够在饱和浓度和碳源的含有L-谷氨酸的液体培养基中在特定pH下代谢碳源的微生物，并且具有将L-谷氨酸的积累量超过对应于 在液体介质中的饱和浓度在pH值。 还提供了通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并沉淀 培养基中的L-谷氨酸。

公开(公告)号：US07205132B2

公开(公告)日：2007-04-17

申请号：US11222138

申请日：2005-09-09

申请人： Seiko Hirano ,  Mikiko Yamaguchi ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Mikiko Yamaguchi ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12Q1/04 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12P13/14 ,  C12N9/0012

摘要： A coryneform bacterium which has an L-glutamic acid-producing ability and grows at least at the same growth rate as a non-mutated strain or a wild-type strain and has intracellular α-ketoglutarate dehydrogenase activity which is less than half that of the non-mutated or wild-type strain, and is obtained by introducing a mutation into a coding region or an expression control region of the chromosomal odhA gene encoding the E1o subunit of the α-ketoglutarate dehydrogenase complex.

摘要翻译： 具有L-谷氨酸生产能力的棒状杆菌型细菌至少以与非突变菌株或野生型菌株相同的生长速率生长，并且具有小于所述野生型菌株的一半的细胞内α-酮戊二酸脱氢酶活性 非突变或野生型菌株，并且通过将突变引入编码α-酮戊二酸脱氢酶复合物的E1o亚基的染色体odhA基因的编码区域或表达控制区而获得。

公开(公告)号：US20060205043A1

公开(公告)日：2006-09-14

申请号：US11275900

申请日：2006-02-02

申请人： Nobuharu Tsujimoto ,  Tomoko Suzuki ,  Hisao Ito

发明人： Nobuharu Tsujimoto ,  Tomoko Suzuki ,  Hisao Ito

IPC分类号： C12P13/04 ,  C12P13/22 ,  C12P13/08 ,  C12N1/21

CPC分类号： C07K14/245 ,  C12P13/04 ,  C12P13/08 ,  C12P13/222

摘要： A method for producing a target substance by utilizing a microorganism by culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture is described. The microorganism is a mutant recombinant strain in which maltose assimilation is controlled by reducing or eliminating the interaction between IIAGlc protein of the glucose PTS and a protein involved in non-PTS uptake of maltose.

摘要翻译： 描述了通过在培养基中培养微生物来生产和积累目标物质并从培养物中收集目标物质的微生物生产目标物质的方法。 微生物是通过减少或消除葡萄糖PTS的IIA蛋白与参与非PTS摄取麦芽糖的蛋白质之间的相互作用来控制麦芽糖同化的突变重组菌株。

公开(公告)号：US20060160191A1

公开(公告)日：2006-07-20

申请号：US11275562

申请日：2006-01-17

申请人： Saori Kataoka ,  Yuuta Nakai ,  Takuji Ueda ,  Yuji Joe ,  Hisao Ito

发明人： Saori Kataoka ,  Yuuta Nakai ,  Takuji Ueda ,  Yuji Joe ,  Hisao Ito

IPC分类号： C12P13/04 ,  C12P13/14 ,  C12P13/08 ,  C12N1/21

CPC分类号： C07K14/245 ,  C12N15/70 ,  C12P13/04 ,  C12P13/08 ,  C12P13/14

摘要： An L-amino acid producing bacterium of the Enterobacteriaceae family is described, wherein the bacterium has been modified so as to not produce type I fimbrial adhesin protein is cultured in a medium to produce and excrete said L-amino acid in the medium, and collecting said L-amino acid from the medium.

摘要翻译： 描述了肠杆菌科的L-氨基酸生产细菌，其中细菌已被修饰以便不产生I型菌毛粘附素蛋白质在培养基中培养以产生并排出培养基中的所述L-氨基酸，并收集 所述L-氨基酸来自培养基。