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273 条记录 (耗时 0.038 秒)

    CORYNEBACTERIUM GLUTAMICUM VARIETY PRODUCING L-ARGININE AND METHOD FOR FABRICATING THE SAME
    71.
    发明申请
    CORYNEBACTERIUM GLUTAMICUM VARIETY PRODUCING L-ARGININE AND METHOD FOR FABRICATING THE SAME 有权
    生产L-阿魏酸的罗丹明葡萄球菌及其制备方法

    公开(公告)号：US20100041109A1

    公开(公告)日：2010-02-18

    申请号：US12522280

    申请日：2008-01-11

    申请人： Hye Won Kim ,  Hyejin Choi ,  Ji-Hye Lee ,  Soo Youn Hwang

    发明人： Hye Won Kim ,  Hyejin Choi ,  Ji-Hye Lee ,  Soo Youn Hwang

    IPC分类号： C12P13/10 ,  C12N1/21

    CPC分类号： C12P13/10

    摘要： The present invention relates to an L-arginine producing mutant strain, and a method for fabricating the same. In particular, the present invention relates to a polynucleotide comprising an argD2 gene (Ncgl2355) that is a putative gene of acetylornithine aminotransferase involved in arginine biosynthesis of Corynebacterium glutamicum, a polypeptide encoded by the polynucleotide, a recombinant vector comprising the polynucleotide, a transformant capable of producing L-arginine in a high yield, which is prepared by introducing the recombinant vector into an L-arginine producing host microorganism to overexpress the argD2 gene, and a method for producing L-arginine by culturing the transformant. The transformant of the present invention overexpresses the argD2 gene to produce L-arginine in a high yield, thereby being used in medicinal and pharmaceutical industries.

    摘要翻译： 本发明涉及L-精氨酸生产突变菌株及其制造方法。 特别地，本发明涉及包含argD2基因（Ncgl2355）的多核苷酸，其是参与谷氨酸棒杆菌的精氨酸生物合成的乙酰鸟氨酸氨基转移酶的推定基因，由该多核苷酸编码的多肽，包含多核苷酸的重组载体，能够转化的转化体 通过将重组载体导入产生L-精氨酸的宿主微生物以过表达argD2基因而制备L-精氨酸，以及通过培养转化体来生产L-精氨酸的方法。 本发明的转化体以高产率过表达argD2基因产生L-精氨酸，从而用于医药和制药工业。

    Method for producing L-amino acid using methylotroph
    75.
    发明授权
    Method for producing L-amino acid using methylotroph 失效
    使用甲基营养生产L-氨基酸的方法

    公开(公告)号：US07439038B2

    公开(公告)日：2008-10-21

    申请号：US10716480

    申请日：2003-11-20

    申请人： Yoshiya Gunji ,  Hisashi Yasueda

    发明人： Yoshiya Gunji ,  Hisashi Yasueda

    IPC分类号： C12P21/06 ,  C12N9/10

    CPC分类号： C07K14/34 ,  C12P13/08 ,  C12P13/10

    摘要： A DNA encoding for a mutant of LysE protein, or a homologous protein thereof, of a coryneform bacterium, wherein the mutant, when introduced into a methanol-assimilating bacterium imparts resistance to L-lysine analogue. The DNA encoding for a mutant of LysE protein, or a homologous protein thereof, is introduced into a methanol-assimilating bacterium to improve L-lysine and L-arginine productivity of the methanol-assimilating bacterium.

    摘要翻译： 编码棒状细菌的LysE蛋白或其同源蛋白质的突变体的DNA，其中所述突变体在被引入甲醇同化细菌中时赋予对L-赖氨酸类似物的抗性。 将编码LysE蛋白或其同源蛋白质的突变体的DNA引入甲醇同化细菌中以改善甲醇同化细菌的L-赖氨酸和L-精氨酸生产率。

    Process for Preparing L-amino Acids
    76.
    发明申请
    Process for Preparing L-amino Acids 审中-公开
    L-氨基酸的制备方法

    公开(公告)号：US20070122832A1

    公开(公告)日：2007-05-31

    申请号：US11615860

    申请日：2006-12-22

    申请人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    发明人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P13/08 ,  C12N9/10 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/1205 ,  C07K14/34 ,  C12N9/10 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/222 ,  C12P13/225 ,  C12P13/227 ,  C12P13/24

    摘要： The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70％ b）编码多肽的多核苷酸，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）的多核苷酸互补的多核苷酸， 或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少有citA基因 以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Enzymatic synthesis of n (alpha)-acyl-l-arginine esters
    79.
    发明申请
    Enzymatic synthesis of n (alpha)-acyl-l-arginine esters 有权
    n（α） - 酰基-1-精氨酸酯的酶合成

    公开(公告)号：US20060003427A1

    公开(公告)日：2006-01-05

    申请号：US10501941

    申请日：2002-02-01

    申请人： Joan Bonaventura ,  Xavier Bonvila

    发明人： Joan Bonaventura ,  Xavier Bonvila

    IPC分类号： C12P13/00

    CPC分类号： C12P13/08 ,  C12P13/04 ,  C12P13/10 ,  C12P13/24

    摘要： The invention relates to a process for preparing a Na-acyl-L-arginine ester, derived from fatty acids and esterified dibasic amino acids, according to the following formula (I), where: X— is Br—, Cl—, or HSO4- R1: is linearalkyl chain from an saturated fatty acid, or hydroxy-acid from 8 to 14 atoms of carbon bonded to the ∝-amino add group through amidic bond. R2: is a linear or branched alkyl chain from 1 to 18 carbon atoms or aromatic. R3: is formula (II), where n can be from 0 to 4, from the appropriate organic acid and alcohol. The process is catalyzed by a hydrolase, more in particular a protease, the protease papain from Carica papaya being highly suitable . In order for the esterification reaction to run as wanted, the process is performed in a low-water-content organic medium. When the highly suitable papain from Carica papaya is used, a water activity of between 0.03 and 0.5 is selected.

    摘要翻译： 本发明涉及根据下式（I）制备衍生自脂肪酸和酯化二元氨基酸的酰基-L-精氨酸酯的方法，其中：X-是Br-，Cl-或HSO 4 -R 1：来自饱和脂肪酸的直链烷基链，或通过酰胺键键合到α-氨基加成基上的8-14个碳原子的羟基酸。 R2：是1至18个碳原子或芳族的直链或支链烷基链。 R3：由适当的有机酸和醇组成的式（II），其中n可为0至4。 该方法由水解酶，更特别是蛋白酶催化，来自木瓜番木瓜的蛋白酶木瓜蛋白酶非常适合。 为了酯化反应按需要运行，该方法在低含水量的有机介质中进行。 当使用来自番木瓜的高度适宜的木瓜蛋白酶时，选择0.03至0.5之间的水分活度。