公开(公告)号：US08026080B2

公开(公告)日：2011-09-27

申请号：US11725040

申请日：2007-03-15

申请人： Xiaobo Wang ,  Yama A. Abassi ,  Xiao Xu ,  Jiangbo Gan

发明人： Xiaobo Wang ,  Yama A. Abassi ,  Xiao Xu ,  Jiangbo Gan

IPC分类号： C12Q1/20 ,  C12Q1/08 ,  C12M1/34

CPC分类号： G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

摘要： The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

摘要翻译： 本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。 在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。 在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件 用于控制设备站和阻抗分析仪。 在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。 在一些优选的方面，设计测定法以研究化合物对细胞的影响，例如细胞毒性测定。 在其它优选方面，设计这些测定法来研究影响细胞对抗原的IgE介导的反应的化合物。

公开(公告)号：US07642068B2

公开(公告)日：2010-01-05

申请号：US11910517

申请日：2006-04-21

申请人： Sandra Steiner ,  Patricia F. Holder ,  George M. Carlone ,  GowriSankar Rajam

发明人： Sandra Steiner ,  Patricia F. Holder ,  George M. Carlone ,  GowriSankar Rajam

IPC分类号： C12Q1/18 ,  C12Q1/00 ,  G01N33/43 ,  G01N33/567 ,  C12Q1/20

CPC分类号： C12Q1/04 ,  C12Q1/14 ,  C12Q1/18 ,  G01N33/52

摘要： This application discloses a multivalent opsonophagocytosis assay that does not rely on counting of bacterial colonies to determine bacteria viability following opsonophagocytosis. Instead, the method uses a metabolic colorimetric indicator to determine if viable bacteria are present. Also disclosed are arrays that can be used to determine the viability of bacteria following opsonophagocytosis.

摘要翻译： 本申请公开了一种多价调理吞噬作用试验，其不依赖于细菌菌落的计数以确定调理吞噬作用后的细菌活力。 相反，该方法使用代谢比色指示剂来确定是否存在活细菌。 还公开了可以用于确定调理吞噬作用后细菌生存力的阵列。

公开(公告)号：US07625719B2

公开(公告)日：2009-12-01

申请号：US11691815

申请日：2007-03-27

申请人： Shiro Kanegasaki ,  Yuji Kikuchi

发明人： Shiro Kanegasaki ,  Yuji Kikuchi

IPC分类号： C12Q1/18 ,  C12Q1/20

CPC分类号： B01L3/502715 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2400/086 ,  G01N15/1456 ,  G01N2015/1486

摘要： A method of using a microsample treatment apparatus and an apparatus for detecting chemotaxis of cells and separating chemotactic cells includes a number of wells that are connected to each other via a part having resistance to fluids. The wells are each provided with tubes for injecting/sucking a sample and, if necessary, tubes for relieving pressure changes at the injection/suction. The tubes have a space in common at the top ends thereof in which a liquid can be held.

摘要翻译： 使用微量样品处理装置和用于检测细胞的趋化性和分离趋化细胞的装置的方法包括通过具有耐流体性的部分彼此连接的多个孔。 每个井都设有用于注入/吸取样品的管子，如果需要的话，管子用于在注入/抽吸时减轻压力变化。 管在其顶端具有共同的空间，其中可以保持液体。

公开(公告)号：US20080166752A1

公开(公告)日：2008-07-10

申请号：US10584098

申请日：2004-12-22

申请人： Mansour Samadpour

发明人： Mansour Samadpour

IPC分类号： C12Q1/20 ,  C12M1/34

CPC分类号： C12M41/36 ,  C12M37/00 ,  G01N1/02 ,  G01N2001/028 ,  G01N2001/383

摘要： Aspects of the present invention provide novel apparatus and methods for sampling microbial organisms present on surfaces. Preferred apparatus comprises: a reservoir suitable for providing microbial collection fluid; a sterilizable sample collection chamber; a sterilizable, integrated collection fluid delivery and collection fluid recovery member, suitable to deliver collection fluid to a target surface, and contemporaneously recover the delivered fluid from the surface; delivery means, in communication with both the reservoir and the integrated member, and operable to aseptically deliver collection fluid from the reservoir to the integrated member; and vacuum means, in communication with both the sample collection chamber and the integrated member, and operable to direct collection fluid, delivered and recovered by the integrated member, to the sample collection a method for rapid, high-throughput sampling of microbial organisms present on surfaces, comprising: delivering sample collection fluid to a target surface, and contemporaneously recovering the delivered fluid from the target surface by means of an integrated collection fluid delivery and collection fluid recovery member; and collecting the recovered sample collection fluid into a sample collection chamber in communication with the integrated member, whereby sample collection is, at least in part, achieved. The inventive methods and apparatus can be applied to both surface sampling and to atmospheric sampling.

摘要翻译： 本发明的方面提供了用于取样表面上存在的微生物的新颖的装置和方法。 优选的装置包括：适于提供微生物收集流体的贮存器; 可灭菌的样品收集室; 可消毒的，集成的收集流体输送和收集液回收构件，适于将收集流体输送到目标表面，同时从表面回收输送的流体; 传送装置，与储存器和整合构件连通，并且可操作地将收集流体从储存器无菌地传送到集成构件; 以及与样品收集室和整合构件两者连通的真空装置，并且可操作以将由集成构件输送和回收的收集流体引导到样品收集器上，用于快速，高通量取样存在于 表面，包括：将样品收集流体输送到目标表面，并且通过集成的收集流体输送和收集流体回收构件同时从所述目标表面回收输送的流体; 以及将回收的样品收集流体收集到与所述整合构件连通的样品收集室中，由此至少部分地实现样品收集。 本发明的方法和装置可以应用于表面采样和大气采样。

公开(公告)号：US20080160562A1

公开(公告)日：2008-07-03

申请号：US12046106

申请日：2008-03-11

申请人： Yuji Hirowatari ,  Katsuko Hara ,  Hakuo Takahashi

发明人： Yuji Hirowatari ,  Katsuko Hara ,  Hakuo Takahashi

IPC分类号： C12Q1/20

CPC分类号： G01N33/942 ,  G01N2800/042 ,  G01N2800/323 ,  G01N2800/347 ,  Y10T436/145555

摘要： A method for assessing arteriosclerosis comprising measuring the serotonin level in whole blood, serum or platelet-rich plasma and rating the serotonin level on such a scale that the lower it is, the more serious the arteriosclerosis is.

摘要翻译： 一种用于评估动脉硬化的方法，包括测量全血，血清或血小板富血浆中的血清素水平，并以如下规模评定血清素水平，其水平越低，动脉硬化越严重。

公开(公告)号：US20080160561A1

公开(公告)日：2008-07-03

申请号：US11908438

申请日：2006-04-04

申请人： Ester Fernandez-Salas ,  Lance E. Steward ,  Kei Roger Aoki

发明人： Ester Fernandez-Salas ,  Lance E. Steward ,  Kei Roger Aoki

IPC分类号： C12Q1/20 ,  C12N5/00

CPC分类号： G01N33/5014 ,  G01N33/5058 ,  G01N33/542 ,  G01N33/56911 ,  G01N33/94

摘要： Compositions useful for detecting Clostridial toxin activity comprising a cell that comprises a membrane-associated Clostridial toxin substrate comprising a first member of a fluorescence resonance energy transfer pair; and a Clostridial toxin recognition sequence including a cleavage site; and a membrane-associated second member of the FRET pair and methods useful for determining Clostridial toxin activity using such Clostridial toxin substrates.

摘要翻译： 用于检测梭菌毒素活性的组合物，其包含细胞，所述细胞包含与所述膜相关的梭菌毒素底物，所述底物包含荧光共振能量转移对的第一成员; 和包含切割位点的梭菌毒素识别序列; 和FRET对的膜相关的第二个成员以及可用于使用这种梭菌毒素底物测定梭菌毒素活性的方法。

公开(公告)号：US20080153110A1

公开(公告)日：2008-06-26

申请号：US11924529

申请日：2007-10-25

申请人： Tim Scherkenback ,  Dan Faubion ,  Yiping Wang

发明人： Tim Scherkenback ,  Dan Faubion ,  Yiping Wang

IPC分类号： G01N33/53 ,  C12M1/34 ,  C12Q1/20

CPC分类号： G01N33/5308 ,  B01L3/5023 ,  B01L2300/069 ,  B01L2300/0825 ,  B01L2400/0406 ,  G01N33/54386 ,  G01N33/569

摘要： Hand-held test cartridges are described herein that comprise at least one assay, where each assay can simultaneously test for at least two different types of biological agents, environmental agents or combinations thereof. Methods of measuring biological and environmental agents are described herein that include: providing at least one multicomponent hand-held test cartridge, wherein the cartridge has multiple channels for testing multiple agents, providing at least two biological agents, environmental agents or a combination thereof, applying the at least two biological agents, environmental agents or a combination thereof to the multicomponent test cartridge, such that the biological and/or environmental agent is applied to the appropriately labeled section of the cartridge.

摘要翻译： 本文描述的手持测试盒包括至少一种测定，其中每个测定可以同时测试至少两种不同类型的生物试剂，环境试剂或其组合。 本文描述了测量生物和环境试剂的方法，其包括：提供至少一种多组分手持式测试盒，其中所述盒具有用于测试多种试剂的多个通道，提供至少两种生物试剂，环境试剂或其组合，应用 所述至少两种生物试剂，环境试剂或其组合与所述多组分测试盒相结合，使得所述生物和/或环境试剂施加到所述盒的适当标记的部分。

公开(公告)号：US20080145879A1

公开(公告)日：2008-06-19

申请号：US11794907

申请日：2006-02-09

申请人： Sylvain Orenga ,  Celine Roger-Dalbert ,  John Perry ,  Vanessa Chanteperdrix ,  Gilles Zambardi ,  Nathalie Bal

发明人： Sylvain Orenga ,  Celine Roger-Dalbert ,  John Perry ,  Vanessa Chanteperdrix ,  Gilles Zambardi ,  Nathalie Bal

IPC分类号： C12Q1/54 ,  C12Q1/04 ,  C12M1/34 ,  C12Q1/20 ,  C12Q1/10 ,  C12Q1/42

CPC分类号： C12Q1/34 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/37 ,  C12Q1/527

摘要： The invention relates to the use of a combination of two culture media for distinguishing at least three groups of microorganisms in a biological sample, comprising: a first group of microorganisms, belonging to a first taxon of microorganisms and comprising at least a first mechanism of resistance to a first treatment; a second group of microorganisms, belonging to a second taxon of microorganisms and comprising at least a second mechanism of resistance to a second treatment; a third group of microorganisms, that are not resistant to said first and second treatments, said combination of two culture media comprising: a. at least a first substrate for detecting at least a first enzymatic or metabolic activity of said first group of microorganisms; b. at least two markers for differentiating the first group of microorganisms and the second group of microorganisms; c. at least one antimicrobial that is active on said third group of microorganisms.

摘要翻译： 本发明涉及两种培养基的组合用于区分生物样品中的至少三组微生物的组合，其包括：属于微生物的第一分类群的第一组微生物，并且至少包含第一抗性机制 第一次治疗; 属于微生物的第二分类单元的第二组微生物，并且包含至少第二种抵抗第二种处理的机制; 第三组微生物，其对所述第一和第二处理不具有抗性，所述两种培养基的组合包括：a。 至少第一底物，用于检测所述第一组微生物的至少第一酶促或代谢活性; b。 用于分化第一组微生物和第二组微生物的至少两种标记物; C。 至少一种在所述第三组微生物上具有活性的抗微生物剂。

公开(公告)号：US20080131905A1

公开(公告)日：2008-06-05

申请号：US12019600

申请日：2008-01-24

申请人： Leroy E. Hood ,  Andrew F. Siegel

发明人： Leroy E. Hood ,  Andrew F. Siegel

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12Q1/20

CPC分类号： G16B40/00 ,  G16B25/00

摘要： The invention provides a method of determining a comparative expression profile in an individual by comparing the expression levels of a sample of molecules in a population of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules, wherein expression levels within the health-associated reference expression region indicate a reference expression profile and wherein expression levels outside the health-associated reference expression region indicate a perturbed expression profile. The invention also provides methods of diagnosing a disease or a health state in an individual by comparing the expression level of a sample of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules. The invention additionally provides a method of classifying a population by drug responsiveness.

摘要翻译： 本发明提供了一种通过将来自个体的标本中的分子群体中的分子样品与分子样品的健康相关参考表达区域的分子样品的表达水平进行比较来确定个体中比较表达谱的方法，其中 健康相关参考表达区域内的表达水平指示参考表达谱，并且其中健康相关参考表达区域之外的表达水平表示扰动的表达谱。 本发明还提供了通过比较来自个体的样品中的分子样品与分子样品的健康相关参考表达区域的表达水平来诊断个体中疾病或健康状态的方法。 本发明另外提供了通过药物反应性对群体进行分类的方法。

公开(公告)号：US20080124745A1

公开(公告)日：2008-05-29

申请号：US11572817

申请日：2005-07-06

申请人： Didier Lefevre

发明人： Didier Lefevre

IPC分类号： G01N33/53 ,  C12Q1/20 ,  C12M1/34 ,  G06G7/48

CPC分类号： G01N15/147 ,  G01N2015/1037 ,  G01N2015/1477 ,  Y10T436/10 ,  Y10T436/101666

摘要： The invention concerns a method for differentiating and counting cellular components present in a biological fluid sample comprising a primary cytological analysis step typically implemented by a flow cytometry equipment (1) to obtain a set of primary results enabling the set of cellular components of the sample to be differentiated and counted in different populations; and a complementary step of cytological analysis of a particular type of cellular components, based on an identified cellular peculiarity, to obtain complementary results enabling at least one cell population or subpopulation of the sample to be differentiated and counted for identification of said cellular peculiarity. The invention is applicable in particular to hematological analyses.

摘要翻译： 本发明涉及用于区分和计数存在于生物流体样品中的细胞成分的方法，其包括通常由流式细胞术设备（1）实施的主要细胞学分析步骤，以获得使得该组样品的细胞组分能够 在不同的人群中进行区分和计数; 以及基于鉴定的细胞特性的特定类型的细胞组分的细胞学分析的补充步骤，以获得使至少一个细胞群体或样品亚群被分化并计数以鉴定所述细胞特性的互补结果。 本发明特别适用于血液学分析。