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    HEPATOCELLULAR CARCINOMA PROTEIN MARKER, AND METHOD FOR DETECTION OF HEPATOCELLULAR CARCINOMA USING THE SAME
    81.
    发明申请
    HEPATOCELLULAR CARCINOMA PROTEIN MARKER, AND METHOD FOR DETECTION OF HEPATOCELLULAR CARCINOMA USING THE SAME 失效
    肝细胞癌蛋白标记物及使用其检测肝细胞癌的方法

    公开(公告)号：US20100248256A1

    公开(公告)日：2010-09-30

    申请号：US12452216

    申请日：2008-06-16

    申请人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

    发明人： Hirotaka Minagawa ,  Kenji Miyazaki ,  Yo Tabuse ,  Kenichi Kamijo ,  Shuichi Kaneko

    IPC分类号： G01N33/53 ,  C07K14/435

    CPC分类号： C07K14/4748 ,  G01N33/57438

    摘要： Provided are: a method of assessing hepatocellular carcinoma by using a protein with a different phosphorylated state in hepatocellular carcinoma cells compared with non-hepatocellular carcinoma cells; and a hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma formed of the protein. The hepatocellular carcinoma protein marker for detecting hepatocellular carcinoma includes tumor rejection antigen gp96 formed of the amino acid represented by SEQ ID NO: 1, and is measured for its phosphorylated state to detect the presence or absence of hepatocellular carcinoma.

    摘要翻译： 提供：与非肝细胞癌细胞相比，通过使用肝细胞癌细胞中具有不同磷酸化状态的蛋白质来评估肝细胞癌的方法; 和用于检测由蛋白质形成的肝细胞癌的肝细胞癌蛋白标记物。 用于检测肝细胞癌的肝细胞癌蛋白标志物包括由SEQ ID NO：1表示的氨基酸形成的肿瘤排斥抗原gp96，并测量其磷酸化状态以检测肝细胞癌的存在或不存在。

    Method of analyzing C-terminal amino acid sequence of peptide
    84.
    发明授权
    Method of analyzing C-terminal amino acid sequence of peptide 失效
    分析肽的C-末端氨基酸序列的方法

    公开(公告)号：US07588944B2

    公开(公告)日：2009-09-15

    申请号：US10589495

    申请日：2004-08-24

    申请人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo

    发明人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo

    IPC分类号： G01N33/00

    CPC分类号： G01N33/6842 ,  G01N33/6821 ,  Y10T436/24

    摘要： An analyte peptide is selectively degraded sequentially by using an alkanoic anhydride (S101). The original peptide and a series of degradation reaction products having peptide in which one or more C-terminal-sided amino acids are deleted, are subjected to a certain posttreatment (S102). The molecular weight of the reaction products is measured by mass spectrometry (S103). And, the amino acid sequence of the original peptide from C-terminal is determined, based on the molecular weight obtained by mass spectrometry (S104).

    摘要翻译： 分析物肽通过使用链烷酸酐依次选择性降解（S101）。 将原来的肽和一系列具有缺失一个或多个C末端氨基酸的肽的降解反应产物进行一定的后处理（S102）。 通过质谱法测定反应产物的分子量（S103）。 并且，基于通过质谱法得到的分子量，测定来自C-末端的原始肽的氨基酸序列（S104）。

    Conductive Paste and Wiring Board Using Same
    85.
    发明申请
    Conductive Paste and Wiring Board Using Same 失效
    导电膏和接线板使用相同

    公开(公告)号：US20090042001A1

    公开(公告)日：2009-02-12

    申请号：US11887844

    申请日：2007-01-18

    申请人： Masahiro Yamakawa ,  Kenji Miyazaki

    发明人： Masahiro Yamakawa ,  Kenji Miyazaki

    IPC分类号： H01B1/22 ,  B32B5/00

    CPC分类号： H05K1/092 ,  H01B1/16 ,  Y10T428/24942

    摘要： A conductive paste contains metal powder, an inorganic binder, and an organic vehicle as main ingredients. The organic vehicle includes a solvent having a boiling point of 270° C. or higher, and the proportion of the solvent with respect to the entire solvent included in the organic vehicle is 3 to 100% by weight.

    摘要翻译： 导电糊包含金属粉末，无机粘合剂和有机载体作为主要成分。 有机载体包括沸点为270℃以上的溶剂，溶剂相对于有机载体中所含的全部溶剂的比例为3〜100重量％。

    Method of identifying protein with the use of mass spectometry
    87.
    发明申请
    Method of identifying protein with the use of mass spectometry 审中-公开
    使用质谱法鉴定蛋白质的方法

    公开(公告)号：US20070148720A1

    公开(公告)日：2007-06-28

    申请号：US10583600

    申请日：2004-12-17

    申请人： Hiroaki Torii ,  Kenji Miyazaki ,  Kenichi Kamijo ,  Akira Tsugita

    发明人： Hiroaki Torii ,  Kenji Miyazaki ,  Kenichi Kamijo ,  Akira Tsugita

    IPC分类号： C12Q1/37 ,  G01N33/00

    CPC分类号： G01N27/62

    摘要： The present invention provides an approach for identifying with high accuracy, a known protein or a variant of the known protein derived from the same genomic gene as in a target protein to be analyzed, based on a mass spectrometric result of a plurality of peptide fragments obtained from site-specific enzymatic digestion of the target protein to be analyzed by referring to nucleotide sequences of genes encoding known proteins on a database and to deduced full-length amino acid sequences thereof. In the approach of the present invention, a candidate known protein of identification is specified with high accuracy by such a process comprising steps of comparing actually measured molecular weight values of the peptide fragments derived from the target protein to be analyzed, which are obtained with the use of peptide fragmentation by the site-specific proteolytic treatment, with predicted molecular weight values of peptide fragments predicted from the deduced full-length amino acid sequences of the known proteins and making comparison in terms of the numbers of matching fragments, the consecutiveness of amino acid sequences of matching fragments of the known protein, and the prediction of variation in a mismatching fragment.

    摘要翻译： 本发明提供了一种基于获得的多个肽片段的质谱结果，高精度鉴定来自与要分析的靶蛋白相同的基因组基因的已知蛋白质或已知蛋白质的变体的方法 通过参考在数据库上编码已知蛋白质的基因的核苷酸序列并推断其全长氨基酸序列来分析待分析的靶蛋白的位点特异性酶促消化。 在本发明的方法中，通过这样的方法以高精度指定候选的已知的鉴定蛋白质，该方法包括以下步骤：比较衍生自待分析的靶蛋白的肽片段的实测值的分子量值， 通过位点特异性蛋白水解处理使用肽断裂，从已知蛋白质的推导的全长氨基酸序列预测的肽片段的预测分子量值，并根据匹配片段的数量进行比较，氨基酸的连续性 已知蛋白质的匹配片段的酸序列，以及错配片段变异的预测。

    Method for analyzing c-terminal amino acid sequence of peptide using mass spectrometry
    88.
    发明申请
    Method for analyzing c-terminal amino acid sequence of peptide using mass spectrometry 有权
    使用质谱分析肽的c末端氨基酸序列的方法

    公开(公告)号：US20060134720A1

    公开(公告)日：2006-06-22

    申请号：US10540814

    申请日：2003-12-25

    申请人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Hiroaki Torii

    发明人： Kenji Miyazaki ,  Akira Tsugita ,  Kenichi Kamijo ,  Hiroaki Torii

    IPC分类号： C12Q1/37

    CPC分类号： G01N33/6821 ,  G01N33/6842 ,  G01N33/6851

    摘要： The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

    摘要翻译： 本发明提供了通过使用连续释放肽的C末端氨基酸的反应来分析肽的C末端氨基酸序列的方法，当连续释放C末端氨基酸的C末端氨基酸时，该方法可以抑制 长氨基酸长度的肽，这种不利的副反应，如在肽的中间位置处的肽键断裂，并且可以在广泛适用的条件下进行化学处理; 在该方法中，将具有长氨基酸长度的肽的干样品预先进行N-酰化处理; 通过使用其中链烷酸酐与少量全氟链烷酸组合的反应试剂，在温和条件下连续释放C-末端氨基酸; 应用水解处理; 然后通过胰蛋白酶消化进行精氨酸残基位点的选择性碎裂; 此后，使用MALDI-TOF-MS装置测量衍生自一系列反应产物的C-末端侧片段的分子量的降低; 鉴定出肽样品的C-末端氨基酸序列。