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88 条记录 (耗时 0.04 秒)

    Multiplexed enzymatic assays
    81.
    发明授权
    Multiplexed enzymatic assays 失效
    多重酶检测

    公开(公告)号：US06630296B2

    公开(公告)日：2003-10-07

    申请号：US09924692

    申请日：2001-08-08

    申请人： Qifeng Xue ,  Ian Gibbons

    发明人： Qifeng Xue ,  Ian Gibbons

    IPC分类号： C12Q100

    CPC分类号： G01N33/561 ,  C12Q1/00 ,  C12Q1/485 ,  G01N2333/9121 ,  G01N2333/91215 ,  G01N2500/04 ,  G01N2500/20 ,  Y10S435/966 ,  Y10S435/968 ,  Y10S435/975

    摘要： A multiplexed enzyme assay method and substrate set for performing the method are disclosed. The method includes performing a plurality of enzyme reactions in the presence of a plurality of enzymes substrates, under conditions effective to convert an enzyme substrate to a corresponding product, where the product of each substrate and the substrate have different separation characteristics from each other and from the other substrates and their corresponding products. After performing the reactions, which may be carried out in separate or combined reactions, the substrates and products in said reactions are separated in a single separation medium. For each separated product and substrate, a separation characteristic effective to identify that product and substrate and a signal related to the amount of the product and substrate is detected. From this, one can determine the amount of substrate converted to the corresponding product in each of the reactions.

    摘要翻译： 公开了一种用于执行该方法的复用酶测定方法和底物组。 该方法包括在有效将酶底物转化成相应产物的条件下，在多个酶底物的存在下进行多个酶反应，其中每个底物和底物的产物彼此具有不同的分离特性 其他基材及其相应的产品。 在进行可以在单独或组合反应中进行的反应之后，所述反应中的底物和产物在单一分离介质中分离。 对于每个分离的产物和底物，检测到有效地鉴定该产物和底物的分离特性和与产物和底物的量相关的信号。 由此，可以确定在每个反应中转化成相应产物的底物的量。

    Apparatus and method for dilution and mixing of liquid samples
    86.
    发明授权
    Apparatus and method for dilution and mixing of liquid samples 失效
    液体样品稀释和混合的装置和方法

    公开(公告)号：US4946795A

    公开(公告)日：1990-08-07

    申请号：US395808

    申请日：1989-08-18

    申请人： Ian Gibbons ,  Robert S. Hillman ,  Channing R. Robertson ,  Jimmy D. Allen

    发明人： Ian Gibbons ,  Robert S. Hillman ,  Channing R. Robertson ,  Jimmy D. Allen

    IPC分类号： B01F13/00 ,  B01F13/08 ,  B01F15/04 ,  G01N1/38 ,  G01N33/04

    CPC分类号： B01F13/0059 ,  B01F13/0818 ,  B01F15/04 ,  G01N1/38 ,  G01N33/04 ,  Y10T436/25625 ,  Y10T436/2575

    摘要： An apparatus for diluting a sample with a diluent, comprising a fixed volume measuring chamber, a fixed volume receiving chamber and fluid receiving relationship to the measuring chamber, a gas vent in the receiving chamber, a stop flow junction between the measuring chamber and the receiving chamber, a sample application site in fluid donating relationship to the measuring chamber, wherein the vertical height difference between the sample application site and the stop flow junction is insufficient to provide a hydrostatic pressure capable of overcoming backpressure at the stop flow junction when sample is applied to the sample application site, and the diluent application site and fluid donating relationship to the measuring chamber. Sample and diluent are added sequentially to the apparatus of the invention without requiring any intervening operations. Various means are provided for restarting flow, which causes diluent to wash the sample into the receiving chamber, where the two can be mixed.

    摘要翻译： 一种用稀释剂稀释样品的装置，包括固定容积测量室，固定容积接收室和与测量室的流体接收关系，接收室中的气体通道，测量室和接收器之间的停止流动接合 室，与测量室的流体供应关系的样品施加部位，其中样品施加部位和止动流动接合点之间的垂直高度差不足以提供当施加样品时能够克服止动流动接合处的背压的静水压力 到样品应用场所，以及稀释剂施用部位和与测量室的流体供给关系。 样品和稀释剂依次添加到本发明的装置中，而不需要任何中间操作。 提供了重新启动流动的各种装置，这使得稀释剂将样品洗涤到接收室中，其中两个可以混合。

    Method and compositions for protecting anerobic microorganisms
    87.
    发明授权
    Method and compositions for protecting anerobic microorganisms 失效
    保护厌氧微生物的方法和组合物

    公开(公告)号：US4775626A

    公开(公告)日：1988-10-04

    申请号：US866824

    申请日：1986-05-23

    申请人： Richard Armenta ,  Ian Gibbons ,  Edwin F. Ullman

    发明人： Richard Armenta ,  Ian Gibbons ,  Edwin F. Ullman

    IPC分类号： C12M1/24 ,  C12M1/04 ,  C12N1/04 ,  C12N1/38

    CPC分类号： C12N1/04 ,  C12M23/08 ,  C12M23/32 ,  C12M23/34 ,  C12M23/38 ,  C12M41/34 ,  Y10S435/801 ,  Y10S435/818

    摘要： A method is disclosed for reducing the oxygen content of a medium in which are present cells, usually anaerobic microorganisms, to extend the time during which the cells remain viable. The method comprises having in fluid contact with the aqueous medium an effective amount of an oxidase and substrate for the oxidase. The oxidase and substrate for the oxidase in an aqueous medium can be in fluid, e.g., air, contact with a separately contained aqueous medium in which the cells are present. Alternatively, the cells can be present in the same aqueous medium as the oxidase and substrate for the oxidase. The aqueous medium containing the oxidase and the substrate for the oxidase can further contain a hydrogen peroxide scavenger in an effective amount. A composition in accordance with the invention comprises in a liquid medium viable cells, such as anaerobic microorganisms, an oxidase and its substrate in an amount sufficient to retain the viability of the cells, and a hydrogen peroxide scavenger in an amount sufficient to reduce the level of hydrogen peroxide generated by the oxidase. The method of the invention has particular application in transport media used for transporting microbiological specimens.

    摘要翻译： 公开了一种减少其中存在细胞（通常是厌氧微生物）延长细胞保持存活时间的培养基的氧含量的方法。 该方法包括与水性介质流体接触有效量的用于氧化酶的氧化酶和底物。 水性介质中氧化酶的氧化酶和底物可以是流体，例如空气，与存在细胞的单独含有的水性介质接触。 或者，细胞可以存在于与氧化酶和氧化酶底物相同的水性介质中。 含有氧化酶的水性介质和氧化酶底物还可以含有有效量的过氧化氢清除剂。 根据本发明的组合物在液体介质中包含足以保持细胞存活力的量的活细胞，例如厌氧微生物，氧化酶及其底物，以及足以降低水平的量的过氧化氢清除剂 的氧化酶产生的过氧化氢。 本发明的方法在用于运输微生物标本的运输介质中具有特殊的应用。

    Homogeneous enzyme specific binding assay on non-porous surface
    88.
    发明授权
    Homogeneous enzyme specific binding assay on non-porous surface 失效
    在无孔表面上的均相酶特异性结合测定

    公开(公告)号：US4629690A

    公开(公告)日：1986-12-16

    申请号：US561987

    申请日：1983-12-14

    申请人： Litai Weng ,  Ian Gibbons ,  Edwin F. Ullman

    发明人： Litai Weng ,  Ian Gibbons ,  Edwin F. Ullman

    IPC分类号： G01N33/542 ,  G01N33/58 ,  G01N33/543

    CPC分类号： G01N33/542 ,  G01N33/581 ,  Y10S435/81 ,  Y10S435/966 ,  Y10S435/975

    摘要： Enzyme channeling assay is provided employing a solid non-porous surface to which is bound a member of a specific binding pair and an enzyme. A complementary member of a specific binding pair is conjugated to second enzyme, so that the amount of second enzyme which becomes bound to said solid non-porous surface by the binding of a specific binding pair(s) is related to the amount of analyte in the liquid assay medium with which the solid surface is in contact. The first and second enzymes are related by the product of one being the substrate of the other. The turnover of the substrate formed by one of the enzymes results in a detectable product, which can be related to the amount of analyte in the medium.

    摘要翻译： 使用结合特异性结合对和酶的成员的固体无孔表面提供酶引导测定。 特异性结合对的互补成员与第二酶结合，使得通过特异性结合对的结合而与所述固体无孔表面结合的第二酶的量与分析物的量相关 与固体表面接触的液体测定介质。 第一和第二酶与另一种酶的产物相关。 由酶之一形成的底物的营业额导致可检测的产物，其可与培养基中分析物的量相关。