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公开(公告)号：US5874276A
公开(公告)日：1999-02-23
申请号：US448873
申请日：1995-05-24
CPC分类号： C12Y302/01004 , A23K20/189 , C12N15/80 , C12N9/2437 , C12Y302/01021 , C12Y302/01091
摘要： The present invention relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in the filamentous fungus Trichoderma longibrachiatum. Further aspects of the present invention relate to fungal transformants that express the novel truncated cellulases and derivatives, and expression vectors comprising the DNA gene fragments or variants thereof that code for the truncated cellulases derived from Trichoderma longibrachiatum using genetic engineering techniques.
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公开(公告)号：US5753484A
公开(公告)日：1998-05-19
申请号：US438870
申请日：1995-05-10
IPC分类号： C12N15/09 , C11D3/386 , C11D11/00 , C12N9/24 , C12N9/42 , C12N15/56 , C12N15/80 , C12R1/885 , D06M16/00 , C07K1/00 , C12N9/00 , C12N9/14
CPC分类号： C11D3/38645 , C12N15/80 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D06M2200/50
摘要： The present invention is directed to purified EG III cellulase enzyme isolated from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms. The present invention is further directed to the DNA fragment and sequence that encodes the EG III cellulase enzyme. Also disclosed are methods for isolating either purified or highly enriched EG III cellulase obtained from Trichoderma spp. or genetically modified strains of Trichoderma spp.
摘要翻译： 本发明涉及从木霉木霉分离的纯化的EG III纤维素酶和分泌（成熟）和非分泌（蛋白质）形式的氨基酸序列。 本发明还涉及编码EG III纤维素酶的DNA片段和序列。 还公开了分离从木霉属（Trichoderma spp）获得的纯化的或高度富集的EG III纤维素酶的方法。 或木霉属的遗传修饰菌株。
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公开(公告)号：US20130224864A1
公开(公告)日：2013-08-29
申请号：US13817421
申请日：2011-08-25
申请人： Timothy C. Dodge , Aleksandra Virag , Michael Ward
发明人： Timothy C. Dodge , Aleksandra Virag , Michael Ward
IPC分类号： C12N15/80
CPC分类号： C12N15/80 , C07K14/37 , C12N1/14 , C12N9/2428 , C12N9/2434 , C12N9/2477 , C12Y302/01003
摘要： Described are compositions and methods relating variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
摘要翻译： 描述了具有改变的生长特征的变异丝状真菌的组合物和方法。 这样的变体非常适合浸没培养物中的生长，例如用于大规模生产用于商业应用的酶和其它蛋白质。
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公开(公告)号：US20130011877A1
公开(公告)日：2013-01-10
申请号：US13472296
申请日：2012-05-15
申请人： Huaming Wang , Michael Ward
发明人： Huaming Wang , Michael Ward
CPC分类号： C07K16/462 , C07K16/32 , C07K16/46 , C07K2319/00 , C12N15/62 , C12N15/80
摘要： The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.
摘要翻译： 本发明涉及包含KEX2区域的融合DNA构建体，所述KEX2区域包含KEX2位点和紧邻KEX2位点5'的KEX2位点前序列，融合多肽，载体和包含融合DNA构建体的细胞，用于产生所需蛋白质的方法 丝状真菌细胞和用于增强从细胞中分泌和/或切割所需蛋白质的方法。
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公开(公告)号：US20120196002A1
公开(公告)日：2012-08-02
申请号：US13265712
申请日：2010-04-23
申请人： Susan Madrid , Cherry Lin , Masoud Rajabi Zargahi , Rikke Høegh Lorentsen , Mai Faurschou Isaksen , Michael Ward
发明人： Susan Madrid , Cherry Lin , Masoud Rajabi Zargahi , Rikke Høegh Lorentsen , Mai Faurschou Isaksen , Michael Ward
CPC分类号： C12N9/20 , A23K20/189 , A23L29/06 , A23L33/14 , A23V2002/00 , C12N15/80 , C12Y301/01003
摘要： The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.
摘要翻译： 本发明提供转化或转染的里氏木霉细胞，其包含以下一种或多种：（i）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列（ ii）至少一个编码脂解酶的异源核苷酸序列，其中所述核苷酸序列包含如SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列，或（iii）至少一个编码脂解酶的异源核苷酸序列，其中 核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列; 并在允许表达编码所述脂肪分解酶的所述异源核苷酸序列的条件下培养细胞。
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公开(公告)号：US20110287511A1
公开(公告)日：2011-11-24
申请号：US13029919
申请日：2011-02-17
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译： 本文描述了从里氏木霉分离的新基因序列。 描述编码包含纤维素结合结构域的蛋白质的两个基因，一个编码阿拉伯糖呋喃糖苷酶的基因和一个编码乙酰木聚糖酯酶的基因。 序列CIP1和CIP2含有纤维素结合结构域。 这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。
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公开(公告)号：US20110136197A1
公开(公告)日：2011-06-09
申请号：US12920688
申请日：2009-03-05
CPC分类号： C12N9/0065 , C12P3/00 , C12Y111/01006
摘要： The invention provides methods for expression of a catalase enzyme in a Trichoderma host cell. In one embodiment, the catR gene from Aspergillus niger is expressed in Trichoderma reesei, resulting in improved yields of catalase enzyme in comparison with expression of catR in A. niger.
摘要翻译： 本发明提供了在木霉属宿主细胞中表达过氧化氢酶的方法。 在一个实施方案中，来自黑曲霉的catR基因在里氏木霉中表达，与黑曲霉中catR的表达相比，导致过氧化氢酶的产量提高。
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公开(公告)号：US07923235B2
公开(公告)日：2011-04-12
申请号：US12683108
申请日：2010-01-06
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译： 本文描述了从里氏木霉分离的新基因序列。 描述编码包含纤维素结合结构域的蛋白质的两个基因，一个编码阿拉伯糖呋喃糖苷酶的基因和一个编码乙酰木聚糖酯酶的基因。 序列CIP1和CIP2含有纤维素结合结构域。 这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。
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公开(公告)号：US20100055731A1
公开(公告)日：2010-03-04
申请号：US12373121
申请日：2007-06-21
申请人： Huaming Wang , Michael Ward
发明人： Huaming Wang , Michael Ward
CPC分类号： C07K16/32 , C07K14/37 , C07K16/46 , C07K2317/56 , C07K2319/00 , C07K2319/50 , C12N15/62 , C12N15/80
摘要： The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.
摘要翻译： 本发明涉及包含KEX2区域的融合DNA构建体，所述KEX2区域包含KEX2位点和紧邻KEX2位点5'的KEX2位点前序列，融合多肽，载体和包含融合DNA构建体的细胞，用于产生所需蛋白质的方法 丝状真菌细胞和用于增强从细胞中分泌和/或切割所需蛋白质的方法。
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公开(公告)号：US20090318667A1
公开(公告)日：2009-12-24
申请号：US12454381
申请日：2009-05-15
申请人： Kent Kirshenbaum , Galia Maayan , Michael Ward
发明人： Kent Kirshenbaum , Galia Maayan , Michael Ward
摘要： Novel peptoids are disclosed that have a formula represented by the following formulae Ia and Ib: wherein X, Y, R, and n are as described herein. The peptoids demonstrate catalytic activity and are useful in substrate-selective catalytic transformations, including asymmetric catalytic transformations.
摘要翻译： 公开了具有由下式Ia和Ib表示的分子式的新型拟肽：其中X，Y，R和n如本文所述。 拟肽显示催化活性，并且可用于底物选择性催化转化，包括不对称催化转化。
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