-
公开(公告)号：US5731424A
公开(公告)日：1998-03-24
申请号：US458424
申请日：1995-06-02
IPC分类号： C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/00 , C07K14/47 , C07K14/49 , C07K14/495 , C07K14/50 , C07K14/52 , C12N9/12 , C12N15/10 , C12N15/115 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： A61K47/48092 , A61K47/48076 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C07K14/47 , C07K14/49 , C07K14/495 , C07K14/50 , C07K14/52 , C12N15/1048 , C12N15/115 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/6842 , G01N33/76 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： Methods are described for the identification and preparation of high-affinity nucleic acid ligands to TGF.beta.. Included in the invention are specific RNA and ssDNA ligands to TGF.beta.1 identified by the SELEX method. Also included are RNA ligands that inhibit the interaction of TGF.beta.1 with its receptor.
摘要翻译： 描述了用于鉴定和制备TGFβ的高亲和力核酸配体的方法。 本发明包括通过SELEX方法鉴定的TGFβ1的特异性RNA和ssDNA配体。 还包括抑制TGFβ1与其受体相互作用的RNA配体。
-
公开(公告)号：US5726017A
公开(公告)日：1998-03-10
申请号：US447172
申请日：1995-05-19
申请人： Michael A. Lochrie , Larry Gold
发明人： Michael A. Lochrie , Larry Gold
IPC分类号： C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/00 , C07K14/47 , C07K14/49 , C07K14/495 , C07K14/50 , C07K14/52 , C12N9/12 , C12N15/10 , C12N15/115 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： A61K47/48092 , A61K47/48076 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C07K14/47 , C07K14/49 , C07K14/495 , C07K14/50 , C07K14/52 , C12N15/1048 , C12N15/115 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/6842 , G01N33/76 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： Methods are described for the identification and preparation of high-affinity nucleic acid ligands to HIV-1 GAG, Included in the invention are specific RNA ligands to HIV-1 GAG identified by the SELEX method, Also included are RNA ligands that inhibit the function of HIV-1 GAG.
摘要翻译： 描述了用于鉴定和制备HIV-1 GAG的高亲和力核酸配体的方法，本发明包括通过SELEX方法鉴定的HIV-1 GAG的特异性RNA配体，还包括抑制功能的RNA配体 HIV-1 GAG。
-
公开(公告)号：US5723592A
公开(公告)日：1998-03-03
申请号：US462389
申请日：1995-06-05
申请人： Bruce E. Eaton , Larry Gold
发明人： Bruce E. Eaton , Larry Gold
IPC分类号： C12N15/09 , A61K31/70 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07H21/02 , C07H21/04 , C07K5/00 , C07K14/00 , C12N9/12 , C12N15/10 , C12N15/115 , C12P19/34 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76
CPC分类号： C07H21/00 , A61K47/48076 , A61K47/48092 , B82Y5/00 , C07H19/06 , C07H19/10 , C07K14/001 , C12N15/1048 , C12N15/115 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/6842 , G01N33/76 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： This invention discloses a method for coevolving products from two or more reactants, along with the nucleic acid that can facilitate the reaction for making the products. The invention further discloses the products and facilitating nucleic acids produced by said method.
-
公开(公告)号：US5712375A
公开(公告)日：1998-01-27
申请号：US434001
申请日：1995-05-03
申请人： Kirk B. Jensen , Hang Chen , Kevin N. Morris , Andrew Stephens , Larry Gold
发明人： Kirk B. Jensen , Hang Chen , Kevin N. Morris , Andrew Stephens , Larry Gold
IPC分类号： A61K38/00 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K7/22 , C07K14/00 , C07K14/16 , C07K14/72 , C12N9/12 , C12N15/10 , C12N15/115 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C07K1/14 , C07H21/04 , C12P19/34
CPC分类号： C07H19/10 , B82Y5/00 , C07H19/06 , C07H21/00 , C07K1/22 , C12N15/1048 , C12N15/115 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , G01N33/53 , G01N33/5308 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/57407 , G01N33/57426 , G01N33/68 , G01N33/76 , G01N33/80 , A61K38/00 , C12N2310/13 , C12N2310/16 , C12N2310/322 , C12N2310/53 , C12N2320/13 , C12Q1/6886 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/4724 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/7056 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： This invention discloses high-affinity oligonucleotide ligands to complex tissue targets, specifically nucleic acid ligands having the ability to bind to complex tissue targets, and the methods for obtaining such ligands. Tissue targets comprise cells, subcellular components, aggregates or cells, collections of cells, and higher ordered structures. Specifically, nucleic acid ligands to red blood cells ghosts, glioblastomas, and lymphomas are described.
-
公开(公告)号：US5696249A
公开(公告)日：1997-12-09
申请号：US409442
申请日：1995-03-24
申请人： Larry Gold , Craig Tuerk
发明人： Larry Gold , Craig Tuerk
IPC分类号： A61K31/70 , A61K47/48 , C07B61/00 , C07H15/12 , C07H19/06 , C07H19/10 , C07H21/00 , C07H21/02 , C07H21/04 , C07K5/00 , C07K14/00 , C07K16/44 , C12N9/12 , C12N15/09 , C12N15/10 , C12N15/115 , C12P19/34 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/48 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/566 , G01N33/569 , G01N33/68 , G01N33/76
CPC分类号： C12N15/115 , A61K47/48076 , A61K47/48092 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C12N15/1048 , C12N9/1276 , C12Q1/37 , C12Q1/6804 , C12Q1/6811 , C12Q1/70 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/6842 , G01N33/76 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： A new class of nucleic acid compounds, referred to as nucleic acid ligands, have been shown to exist that have a specific binding affinity for three dimensional molecular targets. In a preferred embodiment the ligands are identified by the method of the invention referred to as the Systematic Evolution of Ligands by EXponential enrichment (SELEX), wherein a candidate mixture of nucleic acids are iteratively enriched in high affinity nucleic acids and amplified for further partitioning.
摘要翻译： 已经显示存在对三维分子靶具有特异性结合亲和力的新类型的核酸化合物（称为核酸配体）。 在优选的实施方案中，通过本发明的方法鉴定配体，称为通过扩增富集（SELEX）的配体的系统演化，其中核酸的候选混合物迭代地富含高亲和力核酸并被扩增用于进一步分配。
-
86.发明授权
公开(公告)号：US5683867A
公开(公告)日：1997-11-04
申请号：US234997
申请日：1994-04-28
IPC分类号： C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C12N15/10 , C12N15/115 , C12Q1/68 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12N15/115 , A61K47/48076 , A61K47/48092 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C12N15/1048 , C12Q1/6811 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/76 , C40B40/00 , G01N2333/163 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96455 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl (SEQ ID NO:2) ketone. This elastase blended nucleic acid ligand inhibits the biological activity of elastase.
摘要翻译： 描述了一种用于产生含有非核酸功能单元的混合核酸配体的方法。 具体地，将SELEX鉴定的整合素gpIIbIIIa的RNA配体与肽Gly-Arg-Gly-Asp-Thr-Pro（SEQ ID NO：1）缀合。 这种混合RNA配体以高特异性抑制gpIIbIIIa的生物活性。 还描述了与N-甲氧基琥珀酰基-Ala-Ala-Pro-Val-氯甲基（SEQ ID NO：2）酮偶联的弹性蛋白酶的单链DNA配体。 该弹性蛋白酶共混核酸配体抑制弹性蛋白酶的生物活性。
-
公开(公告)号：US5654151A
公开(公告)日：1997-08-05
申请号：US477830
申请日：1995-06-07
申请人： Patrick Nikita Allen , Larry Gold
发明人： Patrick Nikita Allen , Larry Gold
IPC分类号： A61K38/00 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K7/22 , C07K14/00 , C07K14/16 , C07K14/47 , C07K14/72 , C12N9/12 , C12N15/10 , C12N15/115 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C12P19/34
CPC分类号： A61K47/48092 , A61K47/48076 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C07K14/005 , C07K14/47 , C07K14/72 , C07K7/22 , C12N15/1048 , C12N15/115 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , C12Q1/6876 , C12Q1/70 , C12Q1/703 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/6842 , G01N33/76 , A61K38/00 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C12N2740/16222 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： Methods are described for the identification and preparation of high-affinity nucleic acid ligands to HIV-1 nucleocapsid. Included in the invention are specific RNA ligands to HIV-1 nucleocapsid identified by the SELEX method. Also included are RNA ligands that inhibit the function of HIV-1 nucleocapsid.
摘要翻译： 描述了用于鉴定和制备HIV-1核衣壳的高亲和力核酸配体的方法。 本发明包括通过SELEX方法鉴定的HIV-1核衣壳的特异性RNA配体。 还包括抑制HIV-1核衣壳功能的RNA配体。
-
公开(公告)号：US5639868A
公开(公告)日：1997-06-17
申请号：US384708
申请日：1995-02-06
申请人： Nebojsa Janjic , Larry Gold
发明人： Nebojsa Janjic , Larry Gold
IPC分类号： C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/00 , C12N9/12 , C12N15/10 , C12N15/113 , C12N15/115 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C07H21/02 , C07H21/04 , C12Q19/34
CPC分类号： C12N15/115 , A61K47/48076 , A61K47/48092 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C12N15/1048 , C12N15/1136 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/76 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： Methods are described for the identification and preparation of nucleic acid ligand solutions to basic fibroblast growth factor (bFGF). Included in the invention are nucleic acid ligands to bFGF which are inhibitors of bFGF and 2'-amino-modified RNA ligands to bFGF.
摘要翻译： 描述了用于鉴定和制备碱性成纤维细胞生长因子（bFGF）的核酸配体溶液的方法。 本发明中包括bFGF的核酸配体，其是bFGF的抑制剂和bFGF的2'-氨基修饰的RNA配体。
-
公开(公告)号：US5496938A
公开(公告)日：1996-03-05
申请号：US964624
申请日：1992-10-21
申请人： Larry Gold , Craig Tuerk
发明人： Larry Gold , Craig Tuerk
IPC分类号： A61K31/70 , A61K38/00 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07H21/02 , C07H21/04 , C07K5/00 , C07K7/22 , C07K14/00 , C07K14/16 , C07K14/72 , C12N9/12 , C12N15/10 , C12N15/115 , C12P19/34 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76
CPC分类号： A61K47/48092 , A61K47/48076 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C07K14/005 , C07K14/72 , C07K7/22 , C12N15/1048 , C12N15/1058 , C12N15/115 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/6842 , G01N33/76 , A61K38/00 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C12N2740/16222 , C12Q1/703 , C40B40/00 , F02B2075/027 , G01N2333/16 , G01N2333/163 , G01N2333/503 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/918 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： Methods for the identification and production of improved nucleic acid ligands are based on the SELEX process. Nucleic acid ligands to HIV-RT and HIV-1 Rev are identified according to the methods described herein.
摘要翻译： 用于鉴定和产生改良的核酸配体的方法基于SELEX方法。 根据本文所述的方法鉴定HIV-RT和HIV-1 Rev的核酸配体。
-
公开(公告)号：US08975388B2
公开(公告)日：2015-03-10
申请号：US13113261
申请日：2011-05-23
申请人： Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold
发明人： Dominic Zichi , Sheri K. Wilcox , Chris Bock , Daniel J. Schneider , Bruce Eaton , Larry Gold
CPC分类号： C12N15/115 , C12N9/6429 , C12N15/1048 , C12N2310/16 , C12N2310/314 , C12N2310/315 , C12N2310/321 , C12N2310/322 , C12N2310/333 , C12N2310/334 , C12N2310/335 , C12N2310/336 , C12N2330/31 , C12Q1/6811 , C12Q1/6816 , C12Q1/6832 , C12Q1/6834 , G01N33/5308 , G01N33/58 , C12Q2525/205 , C12Q2565/514 , C12Q2525/161 , C12Q2537/101 , C12Q2523/313
摘要： The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.
摘要翻译： 本公开描述了用于产生能够结合靶分子的适配体的改进的SELEX方法，以及用于产生能够结合和共价交联靶分子的光反应性适体的改进的photoSELEX方法。 具体地，本公开描述了用于生产具有比使用先前的SELEX和photoSELEX方法获得的解离速率常数更慢的适体和光致抗体的方法。 本公开进一步描述了具有比使用现有方法获得的那些更快的解离速率常数的适配体和光致抗体。 此外，本公开描述了包括多种功能的适体构建体，包括可切割元件，检测元件和捕获或固定元件。
-
-
-
-
-
-
-
-
-
搜索结果
223 条记录 (耗时 0.03 秒)
