公开(公告)号：US06814937B1

公开(公告)日：2004-11-09

申请号：US09694130

申请日：2000-10-23

申请人： Toshikazu Hirota ,  Takao Ohnishi ,  Yukihisa Takeuchi

发明人： Toshikazu Hirota ,  Takao Ohnishi ,  Yukihisa Takeuchi

IPC分类号： B01L302

CPC分类号： G01N35/00029 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00369 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/022 ,  B01L3/0241 ,  B01L3/0268 ,  B01L2200/027 ,  B01L2400/0439 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  G01N2035/00158 ,  G01N2035/1039 ,  C12Q2565/507

摘要： A dispenser includes a plurality of arranged micropipettes, each including a sample-pouring port for pouring a sample solution from the outside, a cavity for pouring and charging the sample solution thereinto, and a sample discharge port for discharging the sample solution, and formed on at least one or more substrates. The micropipette further includes an actuator section disposed on at least one wall surface of the substrate which forms the cavity so that the sample solution is movable in the cavity, and the sample solution is discharged from the sample discharge port of each of the micropipettes. A pin, which protrudes upwardly, is provided at the sample-pouring port of each of the micropipettes.

摘要翻译： 分配器包括多个布置的微量移液器，每个微量移液器包括用于从外部倾倒样品溶液的样品注入口，用于将样品溶液倒入和填充的腔体，以及用于排出样品溶液的样品排出口，并形成在 至少一个或多个基底。 微量移液管还包括设置在基板的至少一个壁表面上的致动器部分，其形成空腔，使得样品溶液可在空腔中移动，并且样品溶液从每个微量移液管的样品排出口排出。 在每个微量移液管的取样口处设置有向上突出的销。

公开(公告)号：US06776960B2

公开(公告)日：2004-08-17

申请号：US10128777

申请日：2002-04-24

申请人： Toshikazu Hirota ,  Nobuo Takahashi ,  Yukihisa Takeuchi ,  Takao Ohnishi

发明人： Toshikazu Hirota ,  Nobuo Takahashi ,  Yukihisa Takeuchi ,  Takao Ohnishi

IPC分类号： G01N1506

CPC分类号： G01N35/00029 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00369 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/0268 ,  B01L3/5088 ,  B01L7/52 ,  B01L2300/10 ,  B01L2400/0442 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  G01N2035/00158 ,  G01N2035/1039 ,  C12Q2565/507

摘要： Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.

摘要翻译： 公开了一种方法，其包括在基板的表面上形成聚-L-赖氨酸层的预处理步骤，制备含有DNA片段的样品的样品制备步骤，稀释所得样品的浓度的稀释步骤， 以及将稀释的样品溶液供应到基板上以产生DNA芯片的供给步骤。 样品制备步骤包括PCR扩增DNA片段以制备PCR产物的扩增步骤，将获得的PCR产物干燥以形成DNA粉末的粉末形成步骤和将获得的DNA粉末溶解在缓冲溶液中的混合步骤 。

公开(公告)号：US06474566B1

公开(公告)日：2002-11-05

申请号：US09606380

申请日：2000-06-28

申请人： Toshikazu Hirota ,  Takao Ohnishi

发明人： Toshikazu Hirota ,  Takao Ohnishi

IPC分类号： B05B108

CPC分类号： F02M61/1846 ,  B05B17/0607 ,  F02M51/04 ,  F02M51/0603 ,  F02M57/021 ,  F02M57/027 ,  F02M61/166 ,  F02M61/168 ,  F02M61/18 ,  F02M61/1833 ,  F02M61/186 ,  F02M2200/90 ,  F02M2200/9007 ,  F02M2200/9015 ,  F02M2200/9038 ,  F02M2200/9046

摘要： A drop discharge device is provided, including a pressurizing means for achieving discharge of liquid such as raw material or fuel, a pressurizing chamber for pressurizing liquid to be discharged, a nozzle connected to a lower portion of the pressurizing chamber for discharging liquid to a processing unit of the raw material/fuel discharge device, a layer for repelling liquid disposed in a periphery of a discharge hole of the nozzle, and an introducing hole for supplying liquid to the pressurizing chamber. The layer for repelling liquid includes a layer formed to extend over the entire bottom surface of the pressurizing chamber made of fluorocarbon polymers, and grooves for repelling liquid around the discharge hole of the nozzle.

摘要翻译： 一种液滴喷射装置，包括用于实现诸如原料或燃料的液体的排出的加压装置，用于对要排出的液体进行加压的加压室，连接到加压室的下部以将液体排出到加工的喷嘴 原料/燃料排出装置的单元，设置在喷嘴的排出孔周边的排出液的层和向加压室供给液体的导入孔。 用于排斥液体的层包括形成为在由碳氟聚合物制成的加压室的整个底表面上延伸的层，以及用于排斥喷嘴排出孔周围的液体的槽。

公开(公告)号：US06443366B1

公开(公告)日：2002-09-03

申请号：US09685492

申请日：2000-10-10

申请人： Toshikazu Hirota ,  Takao Ohnishi ,  Yukihisa Takeuchi

发明人： Toshikazu Hirota ,  Takao Ohnishi ,  Yukihisa Takeuchi

IPC分类号： B05B108

CPC分类号： B41J2/02

摘要： A liquid-drop discharge device is provided including a float cell provided with a valve and communicating to a liquid storage tank, a liquid discharge mechanism for discharging liquid within the float cell, and a reaction cell provided with a space into which fluid is discharged from the liquid discharge mechanism for collecting the discharged fluid. Air is supplied to the reaction cell for collecting liquid or minute particles, and an internal pressure P1 of the liquid storage tank or the float cell and an internal pressure P3 of the reaction cell are set such that a relationship of P1=P3, in which the pressures are identical to atmospheric pressure, or P1>P3, to supply stronger air, is satisfied.

摘要翻译： 本发明提供一种液滴放电装置，包括具有阀并与液体储存罐连通的浮子池，用于在浮子池内排出液体的液体排出机构，以及设置有流体从其排出的空间的反应池 用于收集排出的流体的液体排出机构。 将空气供给到用于收集液体或微小颗粒的反应池中，并且将液体储存罐或浮子池的内部压力P1和反应池的内部压力P3设定为P1 = P3的关系，其中 压力与大气压力相同，或P1> P3，以提供更强的空气。

公开(公告)号：US06360606B1

公开(公告)日：2002-03-26

申请号：US09267562

申请日：1999-03-12

申请人： Toshikazu Hirota ,  Takao Ohnishi ,  Keizo Miyata ,  Kazuyoshi Shibata

发明人： Toshikazu Hirota ,  Takao Ohnishi ,  Keizo Miyata ,  Kazuyoshi Shibata

IPC分类号： G01N1116

摘要： A piezoelectric sensor device has a piezoelectric body vibrator consisting of the piezoelectric body which is sandwiched by a pair of electrodes, a power source which applies a voltage to the piezoelectric body vibrator so as to get excited for vibration, means for monitoring electric constants to detect changes in electric constants accompanied by vibration of the piezoelectric body. The change in electric constants in the piezoelectric body is detected as a change in frequency for vibration of the piezoelectric body corresponding to an electric constant under the determined conditions. The piezoelectric sensor device has a means to obtain the frequency value from frequencies at not less than two points giving a determined electric constant. According to the piezoelectric sensor device the dispersion in measured values due to change in vibration aptness of vibration system and the polarization state of piezoelectric body vibrator can be made smaller.

公开(公告)号：US06354789B2

公开(公告)日：2002-03-12

申请号：US09732672

申请日：2000-12-08

申请人： Yukihisa Takeuchi ,  Hiroyuki Tsuji ,  Takao Ohnishi

发明人： Yukihisa Takeuchi ,  Hiroyuki Tsuji ,  Takao Ohnishi

IPC分类号： B66C2300

CPC分类号： H01L21/67784 ,  B65G51/03

摘要： An apparatus for feeding semiconductor chips has a structural body having grooves which serve respectively as parallel feed paths for semiconductor chips, the feed paths corresponding to respective quality levels of the semiconductor chips, the structural body being made of partially stabilized zirconia. A stopper mechanism for temporarily stopping semiconductor chips fed along the feed paths comprises piezoelectric bodies disposed in the feed paths in front of terminal walls of the feed paths. A counter mechanism for counting semiconductor chips fed along the feed paths have electrodes disposed in the feed paths near the terminal walls. The apparatus serves as a feed system for floating articles with ejected air and feeding the floated articles, and lends itself to being automatized.

摘要翻译： 用于半导体芯片的馈电装置具有一个具有分别作为半导体芯片的并行馈电路径的槽的结构体，该馈电路径对应于半导体芯片的各个质量水平，该结构体由部分稳定的氧化锆制成。 用于暂时停止沿进给路径供给的半导体芯片的止动机构包括设置在馈送路径的端壁前面的馈送路径中的压电体。 用于计数沿馈送路径馈送的半导体芯片的计数机构具有设置在靠近端壁的馈送路径中的电极。 该装置用作用于具有喷射空气的浮动物品的进料系统，并且供给漂浮物品，并使其自身变得自动化。

公开(公告)号：US06203250B1

公开(公告)日：2001-03-20

申请号：US09212342

申请日：1998-12-15

申请人： Yukihisa Takeuchi ,  Hiroyuki Tsuji ,  Takao Ohnishi

发明人： Yukihisa Takeuchi ,  Hiroyuki Tsuji ,  Takao Ohnishi

IPC分类号： B65G5316

CPC分类号： H01L21/67784 ,  B65G51/03

摘要： An apparatus for feeding semiconductor chips has a structural body having grooves which serve respectively as parallel feed paths for semiconductor chips, the feed paths corresponding to respective quality levels of the semiconductor chips, the structural body being made of partially stabilized zirconia. A stopper mechanism for temporarily stopping semiconductor chips fed along the feed paths comprises piezoelectric bodies disposed in the feed paths in front of terminal walls of the feed paths. A counter mechanism for counting semiconductor chips fed along the feed paths have electrodes disposed in the feed paths near the terminal walls. The apparatus serves as a feed system for floating articles with ejected air and feeding the floated articles, and lends itself to being automatized.