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322 条记录 (耗时 0.043 秒)

    Method for effecting site-directed mutagenesis
    81.
    发明授权
    Method for effecting site-directed mutagenesis 失效
    进行定点突变的方法

    公开(公告)号：US06448048B1

    公开(公告)日：2002-09-10

    申请号：US09214146

    申请日：1998-12-29

    申请人： Jun Tomono ,  Akihiko Kita ,  Susumu Tsunasawa ,  Ikunoshin Kato

    发明人： Jun Tomono ,  Akihiko Kita ,  Susumu Tsunasawa ,  Ikunoshin Kato

    IPC分类号： C12N1564

    CPC分类号： C12N15/102

    摘要： A method for performing site-directed mutagenesis characterized in that the method includes the step of carrying out PCR by the use of a double-stranded DNA vector having one or more amber codons, the vector resulting from insertion of a target DNA fragment for site-directed mutagenesis, and at least two kinds of selection primers; and a kit for site-directed mutagenesis for use in the above method, characterized in that the kit includes amber codon reversion primers. According to the present invention, there can be provided a method for performing site-directed mutagenesis and a kit, which is useful for genetic engineering or protein engineering, more simply and rapidly. By using the method and the kit of the present invention, it is possible to efficiently obtain a mutation-introduced gene at the desired position by simply transforming a host with a PCR product obtained by PCR.

    摘要翻译： 一种用于进行定点诱变的方法，其特征在于该方法包括通过使用具有一个或多个琥珀密码子的双链DNA载体进行PCR的步骤，所述双链DNA载体由插入靶DNA片段所产生的载体， 定向诱变和至少两种选择引物; 以及用于上述方法的用于定点诱变的试剂盒，其特征在于所述试剂盒包括琥珀密码子反转引物。 根据本发明，可以提供用于进行定点诱变的方法和用于遗传工程或蛋白质工程的试剂盒，其更简单和快速。 通过使用本发明的方法和试剂盒，通过用PCR获得的PCR产物简单地转化宿主，可以有效地获得所需位置的突变导入基因。

    Processes of screening for an antimycotic agent
    82.
    发明授权
    Processes of screening for an antimycotic agent 失效
    筛选抗真菌剂的方法

    公开(公告)号：US06432664B1

    公开(公告)日：2002-08-13

    申请号：US09447223

    申请日：1999-11-23

    申请人： Takashi Okado ,  Kazutoh Takesako ,  Ikunoshin Kato

    发明人： Takashi Okado ,  Kazutoh Takesako ,  Ikunoshin Kato

    IPC分类号： C12Q118

    CPC分类号： C07K14/40 ,  A61K38/00 ,  C07K14/37 ,  C07K16/14 ,  Y10S435/822 ,  Y10S435/911 ,  Y10S530/824

    摘要： The invention provides an isolated gene coding for a protein regulating aureobasidin sensitivity; a process for cloning the gene with the use of the gene or a part of the same as a probe; a nucleic acid probe being hybridizable with the gene; an antisense DNA or RNA of the gene; a recombinant or transformant having the gene contained therein; an isolated protein regulating aureobasidin sensitivity and a process for producing the same by using the transformant; an antibody for the protein; a process for detecting the protein or the gene; and a process for screening an antimycotic by using the protein or the transformant. The invention is useful in the diagnosis and treatment of diseases including mycoses.

    摘要翻译： 本发明提供编码调节金黄担子素敏感性蛋白质的分离基因; 使用该基因或其一部分作为探针克隆该基因的方法; 核酸探针可与该基因杂交; 该基因的反义DNA或RNA; 其中含有该基因的重组体或转化体; 调节金丝桃素敏感性的分离蛋白质及其使用该转化体的方法。 蛋白质的抗体; 检测蛋白质或基因的方法; 以及通过使用蛋白质或转化体筛选抗真菌药物的方法。 本发明可用于诊断和治疗包括真菌病的疾病。

    Regulation of aureobasidin sensitivity in fungus
    83.
    发明授权
    Regulation of aureobasidin sensitivity in fungus 有权
    调控真菌中金黄色葡萄球菌敏感性

    公开(公告)号：US06348577B1

    公开(公告)日：2002-02-19

    申请号：US09414006

    申请日：1999-10-07

    申请人： Takashi Okado ,  Kazutoh Takesako ,  Ikunoshin Kato

    发明人： Takashi Okado ,  Kazutoh Takesako ,  Ikunoshin Kato

    IPC分类号： C07K1400

    CPC分类号： C07K14/38 ,  A61K38/00 ,  C07K14/37 ,  C07K14/40 ,  C07K16/14 ,  C12N15/80 ,  C12N15/90 ,  Y10S530/824

    摘要： The present invention provides a protein regulating the sensitivity of fungus to an antimycotic aureobasidin, a gene coding for this protein, the use thereof, an antibody for the protein and the use thereof. The invention is useful in the diagnosis and treatment for diseases including mycoses. The invention also provides a novel chromosome integration vector capable of imparting a novel selective marker of a drug resistance to a fungal transformant, a transformant transformed with this vector and a process for producing the same. In particular, it provides a protein capable of imparting the resistance to aureobasidin and acting as a selective marker and a DNA coding for the same.

    摘要翻译： 本发明提供调节真菌对抗真菌金黄色列生素的敏感性的蛋白质，编码该蛋白质的基因，其用途，蛋白质的抗体及其用途。 本发明可用于包括真菌病在内的疾病的诊断和治疗。 本发明还提供了一种新颖的染色体整合载体，其能够赋予对真菌转化体的耐药性的新型选择性标记，用该载体转化的转化体及其制备方法。 特别地，它提供能够赋予金黄担子素抗性并作为选择性标记的蛋白质和编码它们的DNA。

    Amino-terminal deblocking enzyme
    85.
    发明授权
    Amino-terminal deblocking enzyme 失效
    氨基末端解封酶

    公开(公告)号：US06194190B1

    公开(公告)日：2001-02-27

    申请号：US09202832

    申请日：1998-12-21

    申请人： Yukiko Izu ,  Tetsuki Tanaka ,  Masaru Miyagi ,  Tetsuo Tanigawa ,  Jun Tomono ,  Susumu Tsunasawa ,  Ikunoshin Kato

    发明人： Yukiko Izu ,  Tetsuki Tanaka ,  Masaru Miyagi ,  Tetsuo Tanigawa ,  Jun Tomono ,  Susumu Tsunasawa ,  Ikunoshin Kato

    IPC分类号： C12N950

    CPC分类号： C12N9/48

    摘要： To provide an amino terminal protecting group-releasing enzyme characterized in that the enzyme possesses an activity for releasing a protecting group by acting on a peptide of which amino terminal is blocked by the protecting group, and exhibits the activity for two or more protecting groups, or a functional equivalent thereof; a DNA encoding the same; a method for producing the enzyme; a method for removing amino terminal protecting group including the step of subjecting to a reaction with the enzyme to release amino terminal protecting group; and a method for analyzing an amino acid sequence. The above enzyme is useful in the analysis of an amino acid sequence of peptides, particularly proteins and peptides, of which amino terminal is blocked by unknown protecting groups.

    摘要翻译： 提供氨基末端保护基释放酶，其特征在于该酶具有通过作用于氨基末端被保护基阻断的肽而释放保护基的活性，并表现出两个或更多个保护基的活性， 或其功能等同物; 编码该DNA的DNA; 一种生产该酶的方法; 一种除去氨基末端保护基的方法，包括与酶反应释放氨基末端保护基的步骤; 以及分析氨基酸序列的方法。 上述酶可用于分析肽，特别是蛋白质和肽的氨基酸序列，其氨基末端被未知保护基团阻断。

    Plasmid
    86.
    发明授权
    Plasmid 失效
    质粒

    公开(公告)号：US6165749A

    公开(公告)日：2000-12-26

    申请号：US952089

    申请日：1997-11-10

    申请人： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N15/55 ,  C12N15/69 ,  C12R1/19 ,  C12P21/06

    CPC分类号： C12N15/69 ,  C12N9/1007 ,  C12N9/22

    摘要： A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

    摘要翻译： PCT No.PCT / JP97 / 00748 Sec。 371日期：1997年11月10日 102（e）日期1997年11月10日PCT 1997年3月10日PCT公布。 第WO97 / 34006号公报 日期1997年9月18日一种质粒载体，其特征在于包含可由RNA聚合酶识别的启动子序列，其不是宿主中固有的并且控制所需基因的表达和增加诱导下拷贝数的复制起点 由外生因素; 通过使用载体表达和分离靶基因的方法; 具有AccIII限制性内切核酸酶活性的多肽; 和编码该多肽的DNA。 本发明首次提供能够将编码对宿主具有致死性或有害性的蛋白质的外源性所需基因导入宿主的质粒载体，使用该载体有效表达蛋白质的方法，以及允许限制的方法 构成即使在不存在修饰酶基因的情况下分离的限制性修饰系统的核酸内切酶基因，这在现有技术中是困难的。

    Cyclopentenone derivative
    87.
    发明授权
    Cyclopentenone derivative 失效
    环戊烯酮衍生物

    公开(公告)号：US6136854A

    公开(公告)日：2000-10-24

    申请号：US359069

    申请日：1999-07-21

    申请人： Nobuto Koyama ,  Katsushige Ikai ,  Eiji Kobayashi ,  Ikunoshin Kato

    发明人： Nobuto Koyama ,  Katsushige Ikai ,  Eiji Kobayashi ,  Ikunoshin Kato

    IPC分类号： C07C69/013 ,  A61K31/235 ,  C07C69/52 ,  C07C69/76

    CPC分类号： C07C69/013

    摘要： Cyclopentenone derivative having a structure of 5-(R.sub.1 COO--)-4-(R.sub.2 COO--)--substituted 2-cyclopenten-1-one (R.sub.1 and R.sub.2 are some or different and each is alkyl group, alkenyl group or aryl group) or an optically active substance thereof; a method for the manufacture of the cyclopentenone derivative by the reaction of 4,5-dihydroxy-2-cyclopenten-1-one with the corresponding carboxylic acid or a reactive derivative thereof; and anticancer agent, apoptosis-inducing agent and antibacterial agent containing said derivative.

    摘要翻译： 具有5-（R 1 COO - ） - 4-（R 2 COO - ） - 取代的2-环戊烯-1-酮的结构的环戊烯酮衍生物（R 1和R 2有一些或不同，各自为烷基，烯基或芳基）或 其光学活性物质; 通过4,5-二羟基-2-环戊烯-1-酮与相应的羧酸或其活性衍生物的反应制备环戊烯酮衍生物的方法; 和抗癌剂，凋亡诱导剂和含有所述衍生物的抗菌剂。