公开(公告)号：US20120135517A1

公开(公告)日：2012-05-31

申请号：US13290609

申请日：2011-11-07

申请人： Michael Wayne Graham ,  Robert Norman Rice

发明人： Michael Wayne Graham ,  Robert Norman Rice

IPC分类号： C12N15/85 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N9/0071 ,  C12N9/1051 ,  C12N9/127 ,  C12N9/503 ,  C12N15/113 ,  C12N15/63 ,  C12N15/69 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8283 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/102 ,  C12N2840/20

摘要： The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto.

摘要翻译： 本发明一般涉及用于修饰转基因生物体，特别是转基因动物或植物的细胞，组织或器官中的内源基因表达的合成基因。 更具体地说，本发明提供了新的合成基因和遗传构建体，其能够在引入生物体时抑制延迟或以其他方式降低生物体中的内源基因或靶基因的表达。

公开(公告)号：US08168774B2

公开(公告)日：2012-05-01

申请号：US11218999

申请日：2005-09-02

申请人： Michael Wayne Graham ,  Robert Norman Rice

发明人： Michael Wayne Graham ,  Robert Norman Rice

IPC分类号： C07H21/04

CPC分类号： C12N15/113 ,  A01K2217/05 ,  A61K48/00 ,  C12N9/1051 ,  C12N9/127 ,  C12N9/503 ,  C12N15/111 ,  C12N15/1131 ,  C12N15/63 ,  C12N15/69 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8283 ,  C12N15/85 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/10 ,  C12N2320/30 ,  C12N2330/30 ,  C12N2330/50 ,  C12N2330/51 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/20

摘要： The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endo gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the invention utilizes recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in tissue, organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are cap repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced are also provided.

摘要翻译： 本发明一般涉及修饰基因表达的方法和用于修饰转基因生物体，特别是转基因动物或植物的细胞，组织或器官中的内切基因表达的合成基因。 更具体地，本发明利用重组DNA技术来转录后修饰或调节组织，器官或整个生物体中靶基因的表达，从而产生新的表型。 还提供了当引入时，限制性抑制延迟或以其他方式降低生物体中的内源基因或靶基因的表达的新型合成基因和遗传构建体。

公开(公告)号：US20110209231A1

公开(公告)日：2011-08-25

申请号：US12451540

申请日：2008-05-16

申请人： Timothy James Doran ,  James Climie McKay ,  Robert John Moore ,  John William Lowenthal ,  Scott Geoffrey Tyack

发明人： Timothy James Doran ,  James Climie McKay ,  Robert John Moore ,  John William Lowenthal ,  Scott Geoffrey Tyack

IPC分类号： A01K67/02 ,  A23L1/315

CPC分类号： C12N15/85 ,  A01K2217/058 ,  A01K2227/105 ,  A01K2227/30 ,  A01K2267/0337 ,  C12N15/1131 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/11 ,  C12N2760/16111 ,  C12N2800/90 ,  C12N2830/38 ,  C12N2830/90

摘要： The present invention relates to nucleic acid molecules comprising a double-stranded region, and nucleic acid constructs encoding therfor, that are useful for the treatment and/or prevention of influenza. In particular, the present invention relates to nucleic acid constructs encoding a double stranded RNA molecule(s) that can be used to produce transgenic poultry, for example chickens, such that they are at least less susceptible to an avian influenza infection. Also provided are nucleic acid molecules comprising a double-stranded region that can be used as a therapeutic to treat and/or prevent, for example, avian influenza in poultry.

摘要翻译： 本发明涉及包含双链区域的核酸分子和编码其的核酸构建体，其可用于治疗和/或预防流感。 特别地，本发明涉及编码可用于产生转基因家禽（例如鸡）的双链RNA分子的核酸构建体，使得它们至少不易受禽流感感染的影响。 还提供了包含双链区域的核酸分子，其可以用作治疗和/或预防家禽禽流感的治疗剂。

公开(公告)号：US20110033926A1

公开(公告)日：2011-02-10

申请号：US12884027

申请日：2010-09-16

申请人： Jeffrey S. Chamberlain ,  Dennis J. Hartigan-O'Connor

发明人： Jeffrey S. Chamberlain ,  Dennis J. Hartigan-O'Connor

IPC分类号： C12N5/00 ,  C12N15/63

CPC分类号： C12N15/86 ,  C12N2710/10043 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10351 ,  C12N2800/30 ,  C12N2830/002 ,  C12N2830/38

摘要： The present invention relates to methods and compositions for the production of viral vectors. In particular, the present invention provides methods and compositions for faster, higher titer and higher purity production of viral vectors (e.g. adenoviral vectors). In some embodiments, the present invention provides gutted and helper viruses with identical or similar termini. In other embodiments, the present invention provides terminal protein linked adenoviral DNA. In certain embodiments, the present invention provides template extended adenoviral DNA.

摘要翻译： 本发明涉及用于生产病毒载体的方法和组合物。 特别地，本发明提供了用于更快，更高滴定度和更高纯度产生病毒载体（例如腺病毒载体）的方法和组合物。 在一些实施方案中，本发明提供具有相同或相似末端的内切和辅助病毒。 在其它实施方案中，本发明提供了末端蛋白质连接的腺病毒DNA。 在某些实施方案中，本发明提供扩增模板的腺病毒DNA。

公开(公告)号：US07259009B2

公开(公告)日：2007-08-21

申请号：US10384136

申请日：2003-03-07

申请人： Brian Seed ,  Mason Wright Freeman ,  Alexander Kovtun ,  Masahiro Murakawa ,  Eun-Chung Park ,  Xinzhong Wang

发明人： Brian Seed ,  Mason Wright Freeman ,  Alexander Kovtun ,  Masahiro Murakawa ,  Eun-Chung Park ,  Xinzhong Wang

IPC分类号： C12N15/00 ,  C07H21/04

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N15/63 ,  C12N15/635 ,  C12N2710/10343 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/205 ,  C12N2830/38 ,  C12N2830/85 ,  C12N2840/203 ,  C12N2840/44

摘要： Disclosed are replicatable viral DNA vectors encoding a site-specific DNA-altering enzyme and a DNA target recognized by the enzyme, the enzyme selectively converting, in a cell expressing the enzyme, the DNA vector to a rearranged form. The invention further relates to methods for assembling recombinant adenoviral DNAs. These methods include the steps of: (a) providing a first linearized DNA vector including a restriction site and a cos site and a second linearized DNA vector including the restriction site, an adenoviral nucleic acid molecule, and a cos site; and (b) ligating the first and second linearized DNA vectors, the ligation assembling a recombinant adenoviral DNA.

公开(公告)号：US20070166286A1

公开(公告)日：2007-07-19

申请号：US11717267

申请日：2007-03-13

申请人： Brian Seed ,  Mason Freeman ,  Alexander Kovtun ,  Masahiro Murakawa ,  Eun-Chung Park ,  Xinzhong Wang

发明人： Brian Seed ,  Mason Freeman ,  Alexander Kovtun ,  Masahiro Murakawa ,  Eun-Chung Park ,  Xinzhong Wang

IPC分类号： A61K48/00 ,  C12N15/861

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N15/63 ,  C12N15/635 ,  C12N2710/10343 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/205 ,  C12N2830/38 ,  C12N2830/85 ,  C12N2840/203 ,  C12N2840/44

摘要： Disclosed are replicatable viral DNA vectors encoding a site-specific DNA-altering enzyme and a DNA target recognized by the enzyme, the enzyme selectively converting, in a cell expressing the enzyme, the DNA vector to a rearranged form. The invention further relates to methods for assembling recombinant adenoviral DNAs. These methods include the steps of: (a) providing a first linearized DNA vector including a restriction site and a cos site and a second linearized DNA vector including the restriction site, an adenoviral nucleic acid molecule, and a cos site; and (b) ligating the first and second linearized DNA vectors, the ligation assembling a recombinant adenoviral DNA.

摘要翻译： 公开的是可复制的病毒DNA载体，其编码位点特异性DNA改变酶和被酶识别的DNA靶，该酶在表达该酶的细胞中选择性地转化为重排的形式。 本发明还涉及组装重组腺病毒DNA的方法。 这些方法包括以下步骤：（a）提供包括限制性位点和cos位点的第一线性化DNA载体和包含限制性位点，腺病毒核酸分子和cos位点的第二线性化DNA载体; 和（b）连接第一和第二线性化DNA载体，连接装配重组腺病毒DNA。

公开(公告)号：US07119183B2

公开(公告)日：2006-10-10

申请号：US09836544

申请日：2001-04-17

申请人： Brian Seed ,  Alejandro Aruffo ,  David Camerini

发明人： Brian Seed ,  Alejandro Aruffo ,  David Camerini

IPC分类号： C07H21/04

CPC分类号： C07K14/705 ,  A61K38/00 ,  C07K14/70578 ,  C12N15/1034 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/60 ,  C12N2840/44 ,  G01N33/56972

摘要： A simple and highly efficient method for cloning cDNAs including CD27 (SEQ ID NO:28) from mammalian expression libraries based on transient expression in mammalian host cells has been discovered. Novel expression vectors allowing highly efficient construction of mammalian cDNA libraries are disclosed. The cloning method of the invention which has been used to clone genes for cell surface antigens of human lymphocytes, has general application in gene cloning. Cell surface antigens cloned according to the present invention have been purified, and the nucleotide and amino acid sequences determined. These antigens have diagnostic and therapeutic utility in immune-mediated infections in mammals, including humans.

摘要翻译： 已经发现了一种基于哺乳动物宿主细胞中的瞬时表达来克隆来自哺乳动物表达文库的cDNA（包括CD27（SEQ ID NO：28））的简单且高效的方法。 公开了能够高效构建哺乳动物cDNA文库的新型表达载体。 用于克隆人淋巴细胞表面抗原基因的本发明的克隆方法在基因克隆中具有一般应用。 已经纯化了根据本发明克隆的细胞表面抗原，并确定了核苷酸和氨基酸序列。 这些抗原在哺乳动物（包括人）的免疫介导感染中具有诊断和治疗效用。

公开(公告)号：US20060014715A1

公开(公告)日：2006-01-19

申请号：US11218999

申请日：2005-09-02

申请人： Michael Graham ,  Robert Rice

发明人： Michael Graham ,  Robert Rice

IPC分类号： A61K48/00

CPC分类号： C12N15/113 ,  A01K2217/05 ,  A61K48/00 ,  C12N9/1051 ,  C12N9/127 ,  C12N9/503 ,  C12N15/111 ,  C12N15/1131 ,  C12N15/63 ,  C12N15/69 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8283 ,  C12N15/85 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/10 ,  C12N2320/30 ,  C12N2330/30 ,  C12N2330/50 ,  C12N2330/51 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/38 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/20

摘要： The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endo gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the invention utilises recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in tissue, organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are cap repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced are also provided.

摘要翻译： 本发明一般涉及一种改变基因表达的方法和用于修饰转基因生物体，特别是转基因动物或植物的细胞，组织或器官中内源基因表达的合成基因。 更具体地，本发明利用重组DNA技术来转录后修饰或调节组织，器官或整个生物体中靶基因的表达，从而产生新的表型。 还提供了当引入时，限制性抑制延迟或以其他方式降低生物体中的内源基因或靶基因的表达的新型合成基因和遗传构建体。

公开(公告)号：US20050208021A1

公开(公告)日：2005-09-22

申请号：US11003941

申请日：2004-12-03

申请人： Michele Calos

发明人： Michele Calos

IPC分类号： A61K48/00 ,  C07K14/505 ,  C12N9/00 ,  C12N9/22 ,  C12N15/00 ,  C12N15/52 ,  C12N15/82 ,  C12N15/85 ,  C12N15/86 ,  C12N15/87 ,  C12N15/90

CPC分类号： C12N15/85 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0066 ,  C07K14/505 ,  C12N9/22 ,  C12N15/8209 ,  C12N15/8213 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8273 ,  C12N15/8286 ,  C12N15/90 ,  C12N2800/30 ,  C12N2800/90 ,  C12N2830/002 ,  C12N2830/38 ,  C12N2830/55 ,  C12N2830/85 ,  C12N2840/203 ,  Y02A40/162

摘要： The subject invention provides a unidirectional site-specific integration system for integrating a nucleic acid into the genome of a target cell. The provided systems include a (1) a mutant, unidirectional site specific integrase, which can be provided by an integrase vector encoding the mutant integrase and (2) a targeting vector that includes: (a) a nucleic acid to be integrated; and (b) a vector attachment site, where the targeting vector attachment site serves as a substrate for the mutant, unidirectional site-specific integrase. In using the subject systems for site-specific integration, the targeting vector and integrase are introduced into the target cell and the cell is maintained under conditions sufficient to provide for site-specific integration of the nucleic acid into the target cell genome via a recombination event mediated by the site-specific integrase. Also provided are kits that include the subject systems. The subjects systems, methods and kits find use in a variety of different applications, several representative ones of which are described in detail as well.

摘要翻译： 本发明提供了用于将核酸整合到靶细胞的基因组中的单向位点特异性整合系统。 所提供的系统包括（1）突变单向位点特异性整合酶，其可以由编码突变型整合酶的整合酶载体提供，和（2）靶向载体，其包含：（a）待整合的核酸; 和（b）载体附着位点，其中靶向载体附着位点用作突变单向位点特异性整合酶的底物。 在使用受试系统进行位点特异性整合时，靶向载体和整合酶被引入靶细胞，并且细胞维持在足以通过重组事件提供核酸到靶细胞基因组的位点特异性整合的条件 由位点特异性整合酶介导。 还提供了包括主题系统的套件。 受试者系统，方法和试剂盒可用于各种不同的应用，其中还有几个代表性的这些应用也被详细描述。

公开(公告)号：US06943012B2

公开(公告)日：2005-09-13

申请号：US10106831

申请日：2002-03-25

申请人： Anja Ehrhardt ,  Mark A. Kay

发明人： Anja Ehrhardt ,  Mark A. Kay

IPC分类号： C12N15/09 ,  A61K39/00 ,  C12N5/10 ,  C12N7/00 ,  C12N15/861 ,  C12N15/90 ,  A01N63/00 ,  C07H21/04 ,  C12N1/00 ,  C12N21/06

CPC分类号： C12N15/86 ,  A61K39/00 ,  C12N15/90 ,  C12N2710/10343 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/80 ,  C12N2800/90 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/38 ,  C12N2830/46

摘要： A helper dependent adenoviral vector system is provided. The subject helper dependent adenoviral vector system is made up of: (1) a “gutless” adenoviral vector that include cis-acting human stuffer DNA that provides for in vivo long term, high level expression of a coding sequence present on the vector; (2) an adenoviral helper vector that is characterized by having an adenoviral genome region flanked by recombinase recognition sites, where the helper vectors further include a non-mammalian endonuclease recognition site positioned outside of the adenoviral genome region; and (3) a mammalian cell that expresses the corresponding recombinase and endonuclease, as well as the adenoviral preterminal and polymerase proteins. Also provided are methods of using the subject systems to produce virions having the subject helper dependent adenoviral vectors encapsulated in an adenoviral capsid. In addition, kits for use in practicing the subject methods are provided.

摘要翻译： 提供辅助性依赖性腺病毒载体系统。 受试者辅助因子依赖性腺病毒载体系统由以下部分组成：（1）“无痛”腺病毒载体，其包括顺式作用人填充DNA，其提供载体上存在的编码序列的体内长期高水平表达; （2）腺病毒辅助载体，其特征在于具有侧翼为重组酶识别位点的腺病毒基因组区域，其中辅助载体还包括位于腺病毒基因组区域外部的非哺乳动物内切核酸酶识别位点; 和（3）表达相应的重组酶和内切核酸酶的哺乳动物细胞，以及腺病毒早产和聚合酶蛋白。 还提供了使用受试系统产生具有包封在腺病毒衣壳中的受试者辅助性依赖性腺病毒载体的病毒粒子的方法。 此外，还提供了用于实践主题方法的套件。