Oligonucleotide derivatives
    6.
    发明授权
    Oligonucleotide derivatives 失效
    寡核苷酸衍生物

    公开(公告)号:US4667025A

    公开(公告)日:1987-05-19

    申请号:US790658

    申请日:1985-10-24

    CPC分类号: C07H21/00 Y02P20/55

    摘要: An oligonucleotide derivative having an amino group protected with an eliminatable group bonded through a phosphate group and a spacer with an appropriate length to the 5'-end of an oligonucleotide protected suitably at the 3'-hydroxyl group and the base moiety of the nucleotide, and an immobilized oligonucleotide derivative having a Sepharose carrier bonded to the amino group in place of said protective group are disclosed. Methods for production of these derivatives are also disclosed.

    摘要翻译: 具有通过磷酸基团键合的可消除基团保护的氨基的寡核苷酸衍生物和适当长度的寡核苷酸衍生物,其适当长度保护在核苷酸的3'-羟基和碱基部分适当保护的寡核苷酸的5'-末端, 公开了具有与氨基键合的Sepharose载体代替所述保护基团的固定化寡核苷酸衍生物。 还公开了这些衍生物的制备方法。

    Cells producing recombinant retrovirus
    7.
    发明授权
    Cells producing recombinant retrovirus 失效
    产生重组逆转录病毒的细胞

    公开(公告)号:US6001633A

    公开(公告)日:1999-12-14

    申请号:US952671

    申请日:1998-03-31

    摘要: An objective of the present invention is to provide a packaging cell for preparing a retrovirus having a high viral titer. The cell producing a recombinant retrovirus is constructed by introducing, the Polyoma virus early region gene together with a recombinant plasmid or a recombinant retrovirus free from any replication origin derived from Polyoma virus into a packaging cell for preparing a recombinant retrovirus.

    摘要翻译: PCT No.PCT / JP96 / 01484第 371日期1998年3月31日 102(e)1998年3月31日PCT PCT 1996年5月31日PCT公布。 第WO96 / 38545号公报 日期1996年12月5日本发明的目的是提供一种用于制备具有高病毒滴度的逆转录病毒的包装细胞。 产生重组逆转录病毒的细胞通过将多瘤病毒早期区域基因连同重组质粒或不含任何来源于多瘤病毒的复制起点的重组逆转录病毒一起引入用于制备重组逆转录病毒的包装细胞来构建。

    Detection of HLA-DR
    8.
    发明授权
    Detection of HLA-DR 失效
    HLA-DR的检测

    公开(公告)号:US5939542A

    公开(公告)日:1999-08-17

    申请号:US931072

    申请日:1997-09-15

    IPC分类号: C12Q1/68 C07H21/04

    CPC分类号: C12Q1/6881

    摘要: A probe set is provided which can assay larger amounts of samples in a simpler manner and enables typing of all HLA-DR types currently known to be present in Japanese. The probe set comprises part or all of oligonucleotides having the following sequences 1 to 16 or complementary strands thereof:sequence 1: CGGTTGCTCGGAAAGATGCATCsequence 2: ACACTCCCTCTTTGGCTGsequence 3: GGCCGGGTGGACAACTACsequence 4: ATGTTTAACCTGCTCCAAsequence 5: CCTGATGAGGAGTACTGGAAsequence 6: AGCTACTGCGCTTCGACsequence 7: CGTAGAGTACTCCAAGAAsequence 8: CTTATACTTACCCTGCCAsequence 9: AGACAGGCGGGCCCTsequence 10: TCAAACTTATCCTGCTTCsequence 11: AAACTTAACCTCCTCCAAsequence 12: ACTCTACGTCTGAGTGTCsequence 13: ACGGGTGAGTGTTATTTCsequence 14: GACCTCCTGGAAGACAGGsequence 15: ACATCCTGGAAGACGAGCsequence 16: CCCGTAGTTGTGTCTGCA.

    摘要翻译: 提供了一种探针组,其可以以更简单的方式测定更大量的样品,并且能够打印目前已知以日语存在的所有HLA-DR类型。 探针组包含具有以下序列1至16或其互补链的部分或全部寡核苷酸:序列1:CGGTTGCTCGGAAAGATGCATC序列2:ACACTCCCTCTTTGGCTG序列3:GGCCGGGTGGACAACTAC序列4:ATGTTTAACCTGCTCCAA序列5:CCTGATGAGGAGTACTGGAA序列6:AGCTACTGCGCTTCGAC序列7:CGTAGAGTACTCCAAGAA序列 8:CTTATACTTACCCTGCCA序列9:AGACAGGCGGGCCCT序列10:TCAAACTTATCCTGCTTC序列11:AAACTTAACCTCCTCCAA序列12:ACTCTACGTCTGAGTGTC序列13:ACGGGTGAGTGTTATTTC序列14:GACCTCCTGGAAGACAGG序列15:ACATCCTGGAAGACGAGC序列16:CCCGTAGTTGTGTCTGCA。

    Method for cultivating garlic plants
    9.
    发明授权
    Method for cultivating garlic plants 失效
    栽培大蒜植物的方法

    公开(公告)号:US5913729A

    公开(公告)日:1999-06-22

    申请号:US835489

    申请日:1997-04-08

    申请人: Yoshio Kajimura

    发明人: Yoshio Kajimura

    IPC分类号: A01G1/00 A01G7/00 A01B79/00

    CPC分类号: A01G1/00

    摘要: Efficient methods for cultivating garlic plants are disclosed. Bulbils, which are smaller but available in a larger number per plant than cloves, are used in: a first method for cultivating garlic plant, wherein bulbils of a garlic plant are planted in spring for cultivation after stored for wintering at a temperature not inducing fleshy clove formation; a second method of cultivating garlic plants, wherein a garlic plant in which fleshy clove formation may have been induced (e.g., one which has been exposed to a low temperature in winter) is warmed to negate the state of fleshy clove formation, then the bulbils thereof are planted in spring for cultivation; and a third method of cultivating garlic plants, wherein a garlic plant which has been harvested in the Southern Hemisphere and has not wintered is transported to the Northern Hemisphere, and the bulbils thereof are planted in spring for cultivation. According to the methods of the present invention, a garlic plant which has been planted in spring continues vegetative growth without withering its epigeal part during summer when the day length is long, efficiently yielding bulbs comparable to those obtained by conventional cultivation from cloves.

    摘要翻译: 公开了培育大蒜植物的有效方法。 使用较小但比丁香更多的植物的鳞茎,用于:栽培大蒜植物的第一种方法,其中大蒜植物的鳞茎被种植在春季用于培养,在不诱导肉质的温度下储存以进行越冬 丁香形成; 第二种培养大蒜植物的方法,其中可能诱导肉桂丁香形成的大蒜植物(例如,冬季暴露于低温的大蒜植物)被加热以消除肉丁状腺形成的状态,然后将球茎 春季种植; 以及栽培大蒜植物的第三种方法,其中已经在南半球收获并且没有烧结的大蒜植物被运送到北半球,并且将其鳞茎种植在春季用于培养。 根据本发明的方法,在春季种植的大蒜植物在夏天持续营养生长而不枯萎其表面部分,有效地产生与通过常规栽培从丁香获得的灯泡相当的灯泡。