-
公开(公告)号：US20230242889A1
公开(公告)日：2023-08-03
申请号：US17750758
申请日：2022-05-23
IPC分类号： C12N9/12 , C12N9/00 , C12Q1/6844
CPC分类号： C12N9/1241 , C12N9/00 , C12Q1/6844 , C12Y207/07
摘要： The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.
-
公开(公告)号：US20210180119A1
公开(公告)日：2021-06-17
申请号：US17111869
申请日：2020-12-04
IPC分类号： C12Q1/6844 , C12Q1/6806 , C12N9/12 , B01L7/00 , C12Q1/6818 , G01N33/53
摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.
-
公开(公告)号：US11033894B2
公开(公告)日：2021-06-15
申请号：US16057209
申请日：2018-08-07
发明人： Simon Roderick Grover , Paul Graham Wilkins , Nick David Rollings , Peter Laurence Mayne , Wai Ting Chan , Natalie Frances Scott , Olivier Fernand Flick , Henry Charles Innes , Martyn Gray Darnbrough Beedham , Nicholas David Long , Richard John Hammond
摘要： This disclosure provides systems, apparatuses, and methods for liquid transfer and performing reactions. In one aspect, a system includes a liquid transfer device having a housing having a pipette tip and a plunger assembly; and a reaction chamber, wherein the housing of the liquid transfer device is configured to sealably engage with the reaction chamber. In another aspect, a liquid transfer device including a housing having a pipette tip; and a plunger assembly disposed within the housing and the pipette tip, wherein a portion of the plunger assembly is configured to engage a fluid reservoir such that the plunger assembly remains stationary relative to the fluid reservoir and the housing moves relative to the plunger assembly.
-
公开(公告)号：US11566244B2
公开(公告)日：2023-01-31
申请号：US16703190
申请日：2019-12-04
IPC分类号： C12Q1/68 , C12N15/113 , C12Q1/6816 , C12Q1/6827 , C12Q1/6853 , C12Q1/6844 , C12Q1/6804 , G01N21/78 , G01N33/558 , G01N21/77
摘要： This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
-
公开(公告)号：US11339382B2
公开(公告)日：2022-05-24
申请号：US16155133
申请日：2018-10-09
IPC分类号： C07K14/00 , C12N9/12 , C12N9/00 , C12Q1/6844
摘要： The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.
-
公开(公告)号：US11872554B2
公开(公告)日：2024-01-16
申请号：US16812961
申请日：2020-03-09
发明人： Alexander Schenk zu Schweinsberg , Austin Matthew Derfus , Justin Davidson , Karthikeyan Kumaravadivelu , Maulik Vinod Patel , Olaf Piepenburg , Catherine Jean Greenwood , Oliver Nentwich
CPC分类号： B01L3/502715 , B01L3/5029 , G01N1/14 , G01N35/00069 , B01L2200/027 , B01L2200/082 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0478 , B01L2400/0655 , G01N1/38 , G01N2001/028 , G01N2035/00158 , G01N2035/1034
摘要： A microfluidic device includes an inlet port configured to receive a sample, a first reaction chamber fluidically coupled to the inlet port, a first pump fluidically coupled to the inlet port, a second pump fluidically coupled to a mixing chamber, a metering channel fluidically coupled to the first reaction chamber and to the mixing chamber, and one or more second reaction chambers fluidically coupled to the mixing chamber. The first pump is configured to move fluid from the inlet port to the first reaction chamber and from the first pump to the inlet port. The second pump is configured to move fluid from the second pump to the mixing chamber, from the first reaction chamber to the mixing chamber, and from the mixing chamber to the one or more second reaction chambers.
-
公开(公告)号：US20230039793A1
公开(公告)日：2023-02-09
申请号：US17789762
申请日：2020-12-23
发明人： Austin M. Derfus , Karthikeyan Kumaravadivelu , Neil Quitoviera , Adrian P. Crute , Timothy L. Higby , Richard B. Roth , Aric Joneja , Torsten Gliewe
摘要： Aspects of the present disclosure include sample preparation cartridges including a cylindrical structure and one or more covers. The cylindrical structure further includes a top, a bottom, an annular wall, a plurality of cavities in the annular wall that form a plurality of open-sided chambers on the annular wall and one or more interconnections providing fluidic communication between the plurality of chambers. The one or more covers cover the open side of the plurality of chambers. Also provided is a cylinder housing comprising one or more magnets. The sample preparation cartridge is removably disposed into the cylinder housing or adjacent to the cylinder housing. Methods of using the sample preparation device are also provided.
-
公开(公告)号：US20240216911A1
公开(公告)日：2024-07-04
申请号：US18409552
申请日：2024-01-10
发明人： Alexander Schenk zu Schweinsberg , Austin Matthew Derfus , Justin Davidson , Karthikeyan Kumaravadivelu , Maulik Vinod Patel , Olaf Piepenburg , Catherine Jean Greenwood , Oliver Nentwich
CPC分类号： B01L3/502715 , B01L3/5029 , G01N1/14 , G01N35/00069 , B01L2200/027 , B01L2200/082 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0478 , B01L2400/0655 , G01N2001/028 , G01N1/38 , G01N2035/00158 , G01N2035/1034
摘要： A microfluidic device includes an inlet port configured to receive a sample, a first reaction chamber fluidically coupled to the inlet port, a first pump fluidically coupled to the inlet port, a second pump fluidically coupled to a mixing chamber, a metering channel fluidically coupled to the first reaction chamber and to the mixing chamber, and one or more second reaction chambers fluidically coupled to the mixing chamber. The first pump is configured to move fluid from the inlet port to the first reaction chamber and from the first pump to the inlet port. The second pump is configured to move fluid from the second pump to the mixing chamber, from the first reaction chamber to the mixing chamber, and from the mixing chamber to the one or more second reaction chambers.
-
公开(公告)号：US20240150762A1
公开(公告)日：2024-05-09
申请号：US18538714
申请日：2023-12-13
IPC分类号： C12N15/113 , C12Q1/6804 , C12Q1/6816 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , G01N21/78
CPC分类号： C12N15/113 , C12Q1/6804 , C12Q1/6816 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , G01N21/78 , G01N2021/7759
摘要： This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
-
公开(公告)号：US20240100531A1
公开(公告)日：2024-03-28
申请号：US18274405
申请日：2022-01-28
发明人： Austin M. Derfus , Neil A. Quitoviera , Adrian P. Crute , Carl G. Santavicca , Timothy L. Higby , Armando R. Tovar
IPC分类号： B01L3/00
CPC分类号： B01L3/523 , B01L3/502 , B01L2200/025 , B01L2300/044 , B01L2300/045 , B01L2300/0609 , B01L2300/0672 , B01L2300/0803 , B01L2300/0832 , B01L2400/0478
摘要： Aspects of the present disclosure include a buffer pack for dispensing buffer for sample preparation using a sample preparation cartridge. Also provided are a sealing plate configured for holding the buffer pack and a cap for actuating the buffer pack in the sample preparation cartridge. Aspects of the present disclosure include a sample preparation cartridge that includes the buffer pack and the sealing plate. Aspects of the present disclosure also include a sample preparation cartridge that includes the buffer pack, the sealing plate, and the cap. Methods for filling the buffer pack, assembling a cartridge for use in sample preparation, and using the cartridge for preparing a sample also provided.
-
-
-
-
-
-
-
-
-
搜索结果
18 条记录 (耗时 0.018 秒)
