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    PVA MEMBRANE IMMOBILIZED ENZYME AND PREPARATION METHOD THEREFOR
    2.
    发明公开
    PVA MEMBRANE IMMOBILIZED ENZYME AND PREPARATION METHOD THEREFOR 审中-公开

    公开(公告)号：US20230313169A1

    公开(公告)日：2023-10-05

    申请号：US17998916

    申请日：2021-02-18

    申请人： ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD.

    发明人： Hao HONG ,  Gage JAMES ,  Yi XIAO ,  Na ZHANG ,  Williams VYASA ,  Yuxia CUI ,  Jiadong ZHAO ,  Yanyan GAO

    IPC分类号： C12N11/084 ,  C12N11/04

    CPC分类号： C12N11/084 ,  C12N11/04

    摘要： Described herein are a PVA membrane immobilized enzyme and a preparation method therefor. The PVA membrane immobilized enzyme includes a PVA porous membrane and an enzyme entrapped on the PVA porous membrane. The PVA porous membrane is a three-dimensional structured PVA porous membrane. The enzyme is any one selected from transaminase, D-lactate dehydrogenase, cyclohexanone monooxygenase, ketoreductase, alkene reductase, nitrilase, ammonia lyase, amino acid dehydrogenase, imine reductase, alcohol dehydrogenase, ammonium formate dehydrogenase, glucose 1-dehydrogenase and mutants thereof. The three-dimensional structured PVA porous membrane is used as a carrier to immobilize an enzyme in an entrapment manner. After entrapping and immobilizing the enzyme in the PVA porous membrane, the enzyme is stable, and cannot be easily leached out in the process of use. The PVA porous membrane is suitable for use in continuous flow biochemical catalysis, and has wide applicability to enzymes.

    Transaminase mutant and use thereof
    3.
    发明授权
    Transaminase mutant and use thereof 有权

    公开(公告)号：US11407982B2

    公开(公告)日：2022-08-09

    申请号：US16756649

    申请日：2017-11-06

    申请人： Asymchem Life Science (Tianjin) Co., Ltd

    发明人： Hao Hong ,  Gage James ,  Jiangping Lu ,  Xuecheng Jiao ,  Na Zhang ,  Rui Li ,  Kejian Zhang

    IPC分类号： C12N9/06 ,  C12N9/10 ,  C12P13/00 ,  C12P17/12

    摘要： Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.

    Transaminase mutant and use thereof
    4.
    发明申请
    Transaminase mutant and use thereof 有权

    公开(公告)号：US20220220519A1

    公开(公告)日：2022-07-14

    申请号：US17615262

    申请日：2019-05-30

    申请人： ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD.

    发明人： Hao HONG ,  Gage JAMES ,  Na ZHANG ,  Fang LIU ,  Junjie YAN ,  Ye LIU ,  Zujian WANG

    IPC分类号： C12P13/00 ,  C12N9/10 ,  C12P17/12

    摘要： Provided are a transaminase mutant and a method for producing a chiral amine using the same. An amino acid sequence of the transaminase mutant is an amino acid sequence obtained by mutation occurred in an amino acid sequence as shown in SEQ ID NO: 1, and the mutation includes at least one of the following mutation sites: position 3, position 5, position 8, position 25, position 32, position 45, position 56, position 59, position 60, position 84, position 86, position 164, position 176, position 178, position 180, position 187, position 197, position 206, position 207, position 242, position 245, position 319 and position 324.

    TRANSAMINASE MUTANT AND USE THEREOF
    9.
    发明申请
    TRANSAMINASE MUTANT AND USE THEREOF 有权

    公开(公告)号：US20210024904A1

    公开(公告)日：2021-01-28

    申请号：US16756649

    申请日：2017-11-06

    申请人： Asymchem Life Science (Tianjin) Co., Ltd

    发明人： Hao HONG ,  Gage JAMES ,  Jiangping LU ,  Xuecheng JIAO ,  Na ZHANG ,  Rui LI ,  Kejian ZHANG

    IPC分类号： C12N9/10 ,  C12P13/00 ,  C12P17/12

    摘要： Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.