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    Fibrous matrix for in vitro cell cultivation
    1.
    发明授权
    Fibrous matrix for in vitro cell cultivation 失效
    用于体外细胞培养的纤维基质

    公开(公告)号：US5266476A

    公开(公告)日：1993-11-30

    申请号：US869078

    申请日：1992-04-14

    申请人： Martin Sussman ,  Zvi Bohak ,  Avinoam Kadouri

    发明人： Martin Sussman ,  Zvi Bohak ,  Avinoam Kadouri

    IPC分类号： C12N5/00 ,  C12N5/06 ,  C12N11/08 ,  C12N11/12

    CPC分类号： C12N5/0068 ,  C12M23/08 ,  C12M23/20 ,  C12M25/02 ,  C12M25/14 ,  C12N2533/32 ,  C12N2533/50

    摘要： There is provided a matrix and cultivation system for anchorage dependent cells. The matrix is characterized by a substantially increased available effective surface area for cell attachment which is attained by resorting to the use of a fiber network or open-pore foams with suitable pore size. Attachment of the cells can be enhanced by modifying the surface of the substrate. One embodiment comprises a matrix in particle or flake form.

    摘要翻译： 提供了一种用于锚定依赖细胞的基质和培养系统。 基质的特征在于通过使用具有合适孔径的纤维网或开孔泡沫获得的用于细胞附着的可用有效表面积大大增加。 可以通过改变基底的表面来增强细胞的附着。 一个实施方案包括颗粒或薄片形式的基质。

    Cell culture carriers and method of use
    2.
    发明授权
    Cell culture carriers and method of use 失效
    细胞培养载体及使用方法

    公开(公告)号：US5168058A

    公开(公告)日：1992-12-01

    申请号：US544012

    申请日：1990-06-27

    申请人： Zvi Bohak ,  Avinoam Kadouri ,  Nicholas G. Maroudas

    发明人： Zvi Bohak ,  Avinoam Kadouri ,  Nicholas G. Maroudas

    IPC分类号： C02F3/10 ,  C12M3/04 ,  C12N5/00 ,  C12N5/02

    CPC分类号： C12M29/10 ,  C02F3/10 ,  C12M23/08 ,  C12M25/02 ,  C12N5/0068 ,  Y02W10/15 ,  Y10S261/72

    摘要： The invention relates to packing material for use in the cultivation of anchorage-dependent cells, which require a solid surface for proliferation. The packing material of the invention is provided in the form of units of curved sheet material, which individual units generally have a thickness of about 0.05 mm to 0.25 mm, the other dimensions being of the order of one to a few millimeter maximum dimensions. Various shapes can be used, such as twisted rectangles, segments of cylinders, convulated ribbons, twisted shapes, ring shape and the like.

    摘要翻译： 本发明涉及用于培养锚定依赖性细胞的包装材料，其需要固体表面进行增殖。 本发明的包装材料以弯曲片材的单元的形式提供，单个单元通常具有约0.05mm至0.25mm的厚度，其它尺寸为1至几毫米最大尺寸的数量级。 可以使用各种形状，例如扭曲的矩形，圆柱形部分，带有条带，扭曲形状，环形等。

    Method of preparing a conditioned medium from a confluent stromal cell culture
    3.
    发明授权
    Method of preparing a conditioned medium from a confluent stromal cell culture 有权
    从融合基质细胞培养物制备条件培养基的方法

    公开(公告)号：US07678573B2

    公开(公告)日：2010-03-16

    申请号：US11102623

    申请日：2005-04-11

    申请人： Shoshana Merchav ,  Shai Meretski ,  Dov Zipori ,  Avinoam Kadouri

    发明人： Shoshana Merchav ,  Shai Meretski ,  Dov Zipori ,  Avinoam Kadouri

    IPC分类号： C12N5/00 ,  C12N5/06 ,  C12N5/08 ,  C12N11/08 ,  C12M3/00 ,  A61F2/00

    CPC分类号： C12N5/0647 ,  A61F2/00 ,  A61K2035/124 ,  C12M25/14 ,  C12N5/06 ,  C12N11/08 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/26 ,  C12N2502/1394 ,  C12N2533/30

    摘要： A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells; and (b) when a desired stromal cell density has been achieved, collecting medium from the stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells.

    摘要翻译： 提供了一种制备可用于扩增未分化造血干细胞以增加造血干细胞数量的基质细胞条件培养基的方法。 该方法包括：（a）在固定相插塞生物反应器中在片状的基底上连续流动下建立基质细胞培养物，所述基质包括形成生理上可接受的三维网络的无纺纤维基质 的纤维，从而扩大未分化的造血干细胞; 和（b）当已经实现所需的基质细胞密度时，从固定相插塞生物反应器收集培养基，从而获得可用于扩增未分化造血干细胞的基质细胞条件培养基。

    Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells
    5.
    发明申请
    Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells 审中-公开
    维持和扩大造血干细胞和/或祖细胞的方法和装置

    公开(公告)号：US20090004738A1

    公开(公告)日：2009-01-01

    申请号：US12230566

    申请日：2008-09-02

    申请人： Shoshana Merchav ,  Shai Meretski ,  Dov Zipori ,  Avinoam Kadouri

    发明人： Shoshana Merchav ,  Shai Meretski ,  Dov Zipori ,  Avinoam Kadouri

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0647 ,  A61F2/00 ,  A61K2035/124 ,  C12M25/14 ,  C12N5/06 ,  C12N11/08 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/26 ,  C12N2502/1394 ,  C12N2533/30

    摘要： A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells; and (b) when a desired stromal cell density has been achieved, collecting medium from the stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells.

    摘要翻译： 提供了一种制备可用于扩增未分化造血干细胞以增加造血干细胞数量的基质细胞条件培养基的方法。 该方法包括：（a）在固定相插塞生物反应器中在片状的基底上连续流动下建立基质细胞培养物，所述基质包括形成生理上可接受的三维网络的无纺纤维基质 的纤维，从而扩大未分化的造血干细胞; 和（b）当已经实现所需的基质细胞密度时，从固定相插塞生物反应器收集培养基，从而获得可用于扩增未分化造血干细胞的基质细胞条件培养基。

    Bioreactor
    6.
    发明授权
    Bioreactor 失效
    生物反应器

    公开(公告)号：US5705390A

    公开(公告)日：1998-01-06

    申请号：US422626

    申请日：1995-04-14

    申请人： Avinoam Kadouri ,  Yehoshua Aloni ,  Mordechai Geron

    发明人： Avinoam Kadouri ,  Yehoshua Aloni ,  Mordechai Geron

    IPC分类号： C12M1/00 ,  C12M3/00 ,  C12M3/02 ,  C12M1/10 ,  C12N5/00

    CPC分类号： C12M25/16 ,  C12M27/14 ,  C12M27/22 ,  C12M29/04 ,  C12M41/00

    摘要： A radial flow bioreactor comprising a reaction vessel having an inlet and an outlet respectively for adding and withdrawing a culture medium and at least one cell basket within the reaction vessel for accommodating a plurality of cell carriers and having side wall perforations so dimensioned as to prevent passage of the cell carriers therethrough.

    摘要翻译： 径向流生物反应器，包括具有分别用于添加和取出培养基的入口和出口的反应容器，以及反应容器内的至少一个细胞篮，用于容纳多个细胞载体并具有侧壁穿孔，其尺寸设计成防止通过 的细胞载体。

    METHOD AND APPARATUS FOR MAINTENANCE AND EXPANSION OF HEMOPOIETIC STEM CELLS AND/OR PROGENITOR CELLS
    7.
    发明申请
    METHOD AND APPARATUS FOR MAINTENANCE AND EXPANSION OF HEMOPOIETIC STEM CELLS AND/OR PROGENITOR CELLS 审中-公开
    用于维持和扩大HEMOPOIETIC干细胞和/或前列腺细胞的方法和装置

    公开(公告)号：US20120122220A1

    公开(公告)日：2012-05-17

    申请号：US13360068

    申请日：2012-01-27

    申请人： Shoshana Merchav ,  Shai Meretski ,  Dov Zipori ,  Avinoam Kadouri

    发明人： Shoshana Merchav ,  Shai Meretski ,  Dov Zipori ,  Avinoam Kadouri

    IPC分类号： C12N5/0789 ,  C12N5/071 ,  C12M3/00

    CPC分类号： C12N5/0647 ,  A61F2/00 ,  A61K2035/124 ,  C12M25/14 ,  C12N5/06 ,  C12N11/08 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/26 ,  C12N2502/1394 ,  C12N2533/30

    摘要： A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells ; and (b) when a desired stromal cell density has been achieved, collecting medium from the stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells.

    摘要翻译： 提供了一种制备可用于扩增未分化造血干细胞以增加造血干细胞数量的基质细胞条件培养基的方法。 该方法包括：（a）在固定相插塞生物反应器中在片状的基底上连续流动下建立基质细胞培养物，所述基质包括形成生理上可接受的三维网络的无纺纤维基质 的纤维，从而扩大未分化的造血干细胞; 和（b）当已经实现所需的基质细胞密度时，从固定相插塞生物反应器收集培养基，从而获得可用于扩增未分化造血干细胞的基质细胞条件培养基。

    Method and apparatus for anchorage and suspension cell culture
    9.
    发明授权
    Method and apparatus for anchorage and suspension cell culture 失效
    锚定和悬浮细胞培养的方法和装置

    公开(公告)号：US5501971A

    公开(公告)日：1996-03-26

    申请号：US346830

    申请日：1994-11-30

    申请人： David Freedman ,  Guozheng Wang ,  Avinoam Kadouri

    发明人： David Freedman ,  Guozheng Wang ,  Avinoam Kadouri

    IPC分类号： C12M3/00 ,  C12M1/02 ,  C12M1/40 ,  C12M3/02 ,  C12N5/06 ,  C12N5/00

    CPC分类号： C12M25/18 ,  C12M23/34 ,  C12M27/02 ,  C12M41/26

    摘要： A method and apparatus cultivates anchorage and nonanchorage cell in a reactor. A reactor includes a basket type packed bed and an internal recirculation device. The basket includes a top and bottom portion for allowing medium to flow uniformly through the basket. Medium flows downwardly from the top of the basket through the basket for providing gentle flow and preventing removal of cells from the carrier. Preferably, the carrier is formed of a fiber matrix.

    摘要翻译： 在反应器中培养锚固和非锚定槽的方法和装置。 反应器包括篮式填充床和内部再循环装置。 篮子包括用于允许介质均匀地流过篮子的顶部和底部。 介质从篮顶通过篮子向下流动，以提供缓和的流动并防止细胞从载体中移出。 优选地，载体由纤维基质形成。