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公开(公告)号:US09216392B2
公开(公告)日:2015-12-22
申请号:US12962507
申请日:2010-12-07
申请人: Benjamin Joseph Hindson , Kevin Dean Ness , Billy Wayne Colston, Jr. , Fred Paul Milanovich , Donald Arthur Masquelier , Anthony Joseph Makarewicz, Jr.
发明人: Benjamin Joseph Hindson , Kevin Dean Ness , Billy Wayne Colston, Jr. , Fred Paul Milanovich , Donald Arthur Masquelier , Anthony Joseph Makarewicz, Jr.
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: System, including apparatus and methods, for forming an array of emulsions. The system may comprise a plate including an array of emulsion production units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The system also may comprise a vacuum or pressure source configured to be connected operatively to wells of the plate to form a pressure drop between the input wells and the output well of each unit that is capable of driving a first fluid and a second fluid from respective first and second input wells of such unit and through the droplet generator, for collection as an emulsion in the output well of such unit.
摘要翻译: 用于形成乳剂阵列的系统,包括装置和方法。 该系统可以包括包括乳液生产单元阵列的板。 每个单元可以包括至少一个第一输入井,第二输入井和通过形成液滴发生器的通道连接到第一和第二输入井的输出井。 该系统还可以包括被配置为可操作地连接到板的孔的真空或压力源,以在每个单元的输入孔和输出孔之间形成压力降,该压降能够驱动来自相应的第一流体和第二流体 这种单元的第一和第二输入孔,并通过液滴发生器,用于在该单元的输出井中作为乳液收集。
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公开(公告)号:US09623384B2
公开(公告)日:2017-04-18
申请号:US12962511
申请日:2010-12-07
申请人: Benjamin Joseph Hindson , Kevin Dean Ness , Billy Wayne Colston, Jr. , Fred Paul Milanovich , Donald Arthur Masquelier , Anthony Joseph Makarewicz, Jr.
发明人: Benjamin Joseph Hindson , Kevin Dean Ness , Billy Wayne Colston, Jr. , Fred Paul Milanovich , Donald Arthur Masquelier , Anthony Joseph Makarewicz, Jr.
IPC分类号: B01F3/08 , C12M3/00 , B01F13/00 , B01L3/02 , B01L3/00 , G01N21/3563 , C12Q1/68 , G01N21/64 , B01L7/00
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: System, including apparatus and methods, for performing droplet-based assays. The system may comprise a droplet transporter configured to pick up droplets from each emulsion of an array of reacted emulsions and to drive flow of the droplets through a detection region. The system also may comprise a detector configured to collect data related to one or more analytes from individual droplets of the reacted emulsions as such individual droplets travel through the detection region. The system further may comprise a controller programmed to determine, based on the data collected, an aspect of the one or more analytes in one or more samples included in droplets of the emulsions.
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公开(公告)号:US09156010B2
公开(公告)日:2015-10-13
申请号:US12586626
申请日:2009-09-23
申请人: Billy Wayne Colston, Jr. , Benjamin Joseph Hindson , Kevin Dean Ness , Donald Arthur Masquelier , Fred Paul Milanovich , Douglas N. Modlin , Vincent Riot , Samuel Burd , Anthony Joseph Makarewicz, Jr. , Phillip Belgrader
发明人: Billy Wayne Colston, Jr. , Benjamin Joseph Hindson , Kevin Dean Ness , Donald Arthur Masquelier , Fred Paul Milanovich , Douglas N. Modlin , Vincent Riot , Samuel Burd , Anthony Joseph Makarewicz, Jr. , Phillip Belgrader
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: Systems, including apparatus and methods, for performing assays. These systems may involve separating sample components by partitioning them into droplets or other partitions, amplifying or otherwise reacting the components within the droplets, detecting the amplified components, or characteristics thereof, and/or analyzing the resulting data, among others.
摘要翻译: 用于进行测定的系统,包括装置和方法。 这些系统可以包括通过将样品组分分成液滴或其它分隔,放大或以其他方式使液滴内的组分反应,检测放大的组分或其特征,和/或分析所得数据等来分离样品组分。
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公开(公告)号:US20110092392A1
公开(公告)日:2011-04-21
申请号:US12962507
申请日:2010-12-07
申请人: Billy Wayne Colston, JR. , Donald Arthur Masquelier , Kevin Dean Ness , Anthony Joseph Makarewicz, JR. , Benjamin Joseph Hindson , Fred Paul Milanovich
发明人: Billy Wayne Colston, JR. , Donald Arthur Masquelier , Kevin Dean Ness , Anthony Joseph Makarewicz, JR. , Benjamin Joseph Hindson , Fred Paul Milanovich
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: System, including apparatus and methods, for forming an array of emulsions. The system may comprise a plate including an array of emulsion production units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The system also may comprise a vacuum or pressure source configured to be connected operatively to wells of the plate to form a pressure drop between the input wells and the output well of each unit that is capable of driving a first fluid and a second fluid from respective first and second input wells of such unit and through the droplet generator, for collection as an emulsion in the output well of such unit.
摘要翻译: 用于形成乳剂阵列的系统,包括装置和方法。 该系统可以包括包括乳液生产单元阵列的板。 每个单元可以包括至少一个第一输入井,第二输入井和通过形成液滴发生器的通道连接到第一和第二输入井的输出井。 该系统还可以包括被配置为可操作地连接到板的孔的真空或压力源,以在每个单元的输入孔和输出孔之间形成压力降,该压降能够驱动来自相应的第一流体和第二流体 这种单元的第一和第二输入孔,并通过液滴发生器,用于在该单元的输出井中作为乳液收集。
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公开(公告)号:US20110092376A1
公开(公告)日:2011-04-21
申请号:US12962502
申请日:2010-12-07
申请人: Billy Wayne Colston, JR. , Donald Arthur Masquelier , Kevin Dean Ness , Anthony Joseph Makarewicz, JR. , Benjamin Joseph Hindson , Fred Paul Milanovich
发明人: Billy Wayne Colston, JR. , Donald Arthur Masquelier , Kevin Dean Ness , Anthony Joseph Makarewicz, JR. , Benjamin Joseph Hindson , Fred Paul Milanovich
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: System, including method and apparatus, for performing droplet-based assays. In the method, an emulsion may be obtained that includes droplets packed closely together in a three-dimensional packing arrangement. Data related to an analyte may be collected from individual droplets of the reacted emulsion as such droplets travel serially through a detection region.
摘要翻译: 用于执行基于液滴的测定的系统,包括方法和装置。 在该方法中,可以获得包含三维包装装置中密集在一起的液滴的乳液。 与分析物相关的数据可以从反应的乳液的单个液滴中收集,因为这样的液滴通过检测区域连续行进。
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公开(公告)号:US20110086780A1
公开(公告)日:2011-04-14
申请号:US12963523
申请日:2010-12-08
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: A system, including method and apparatus, for forming an array of emulsions. The system may include a plate providing an array of emulsion production units each configured to produce a separate emulsion and each including a set of wells interconnected by channels that intersect to form a site of droplet generation. Each set of wells, in turn, may include (1) at least one first input well to receive a continuous phase, (2) a second input well to receive a dispersed phase, and (3) an output well configured to receive from the site of droplet generation an emulsion of droplets of the dispersed phase disposed in the continuous phase.
摘要翻译: 一种用于形成乳剂阵列的系统,包括方法和装置。 该系统可以包括提供乳液生产单元阵列的板,每个乳液生产单元被配置为产生单独的乳液,并且每个包括通过相交以形成液滴产生位点的通道相互连接的一组孔。 每组井又可包括(1)至少一个用于接收连续相的第一输入井,(2)第二输入井以接收分散相,以及(3)输出孔,其构造成从 产生液滴的位置是分散相的液滴的乳液,其设置在连续相中。
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公开(公告)号:US20100173394A1
公开(公告)日:2010-07-08
申请号:US12586626
申请日:2009-09-23
申请人: Billy Wayne Colston, JR. , Benjamin Joseph Hindson , Kevin Dean Ness , Donald Arthur Masquelier , Fred Paul Milanovich , Douglas N. Modlin , Vincent Riot , Samuel Burd , Anthony Joseph Makarewicz, JR. , Phillip Belgrader
发明人: Billy Wayne Colston, JR. , Benjamin Joseph Hindson , Kevin Dean Ness , Donald Arthur Masquelier , Fred Paul Milanovich , Douglas N. Modlin , Vincent Riot , Samuel Burd , Anthony Joseph Makarewicz, JR. , Phillip Belgrader
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: Systems, including apparatus and methods, for performing assays. These systems may involve separating sample components by partitioning them into droplets or other partitions, amplifying or otherwise reacting the components within the droplets, detecting the amplified components, or characteristics thereof, and/or analyzing the resulting data, among others.
摘要翻译: 用于进行测定的系统,包括装置和方法。 这些系统可以包括通过将样品组分分成液滴或其它分隔,放大或以其他方式使液滴内的组分反应,检测放大的组分或其特征,和/或分析所得数据等来分离样品组分。
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公开(公告)号:US09243288B2
公开(公告)日:2016-01-26
申请号:US13250815
申请日:2011-09-30
申请人: Kevin Dean Ness , Samuel Burd , Benjamin Joseph Hindson , Phillip Belgrader , Billy W. Colston, Jr.
发明人: Kevin Dean Ness , Samuel Burd , Benjamin Joseph Hindson , Phillip Belgrader , Billy W. Colston, Jr.
IPC分类号: C12Q1/68 , C12M3/00 , B01F3/08 , B01F13/00 , B01L3/02 , B01L3/00 , G01N21/3563 , G01N21/64 , B01L7/00
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: Systems, including apparatus, methods, compositions, kits, and software, for preparing, reacting, detecting, and/or analyzing samples in droplet-based assay systems, among others. The disclosure emphasizes, but is not limited to, a disposable cartridge with lysis chamber and droplet chamber, particularly for use in droplet-based assays.
摘要翻译: 用于制备,反应,检测和/或分析基于液滴的测定系统中的样品的装置,方法,组合物,试剂盒和软件等。 本公开强调但不限于具有裂解室和液滴室的一次性盒,特别用于基于液滴的测定。
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公开(公告)号:US20120028311A1
公开(公告)日:2012-02-02
申请号:US13250815
申请日:2011-09-30
申请人: Billy Wayne Colston, JR. , Benjamin Joseph Hindson , Kevin Dean Ness , Samuel Burd , Phillip Belgrader
发明人: Billy Wayne Colston, JR. , Benjamin Joseph Hindson , Kevin Dean Ness , Samuel Burd , Phillip Belgrader
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: Systems, including apparatus, methods, compositions, kits, and software, for preparing, reacting, detecting, and/or analyzing samples in droplet-based assay systems, among others. The disclosure emphasizes, but is not limited to, a disposable cartridge with lysis chamber and droplet chamber, particularly for use in droplet-based assays.
摘要翻译: 用于制备,反应,检测和/或分析基于液滴的测定系统中的样品的装置,方法,组合物,试剂盒和软件等。 本公开强调但不限于具有裂解室和液滴室的一次性盒,特别用于基于液滴的测定。
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公开(公告)号:US20110092373A1
公开(公告)日:2011-04-21
申请号:US12962511
申请日:2010-12-07
申请人: Billy Wayne Colston, JR. , Donald Arthur Masquelier , Kevin Dean Ness , Anthony Joseph Makarewicz, JR. , Benjamin Joseph Hindson , Fred Paul Milanovich
发明人: Billy Wayne Colston, JR. , Donald Arthur Masquelier , Kevin Dean Ness , Anthony Joseph Makarewicz, JR. , Benjamin Joseph Hindson , Fred Paul Milanovich
CPC分类号: B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要: System, including apparatus and methods, for performing droplet-based assays. The system may comprise a droplet transporter configured to pick up droplets from each emulsion of an array of reacted emulsions and to drive flow of the droplets through a detection region. The system also may comprise a detector configured to collect data related to one or more analytes from individual droplets of the reacted emulsions as such individual droplets travel through the detection region. The system further may comprise a controller programmed to determine, based on the data collected, an aspect of the one or more analytes in one or more samples included in droplets of the emulsions.
摘要翻译: 用于执行基于液滴的测定的系统,包括装置和方法。 系统可以包括液滴传送器,其被配置为从反应的乳液阵列的每个乳液中吸取液滴并且驱动液滴通过检测区域的流动。 该系统还可以包括检测器,其被配置为当这些单个液滴穿过检测区域时,从反应的乳液的各个液滴中收集与一种或多种分析物相关的数据。 该系统还可包括控制器,其被编程为基于所收集的数据,确定包含在乳液液滴中的一个或多个样品中的一种或多种分析物的一个方面。
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