公开(公告)号：US10150968B2

公开(公告)日：2018-12-11

申请号：US13589584

申请日：2012-08-20

申请人： Danielle Marie Mitchell ,  Leon F. Garcia-Martinez ,  Patricia Dianne McNeill ,  Ethan Wayne Ojala ,  Mehmet Inan ,  John Latham

发明人： Danielle Marie Mitchell ,  Leon F. Garcia-Martinez ,  Patricia Dianne McNeill ,  Ethan Wayne Ojala ,  Mehmet Inan ,  John Latham

IPC分类号： C12N1/16 ,  C07K16/00 ,  C12N15/00 ,  C12N15/81 ,  C12P21/00 ,  C12P21/02

摘要： Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yield and decreased production of undesired side-products. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.

公开(公告)号：US09321357B2

公开(公告)日：2016-04-26

申请号：US13436290

申请日：2012-03-30

申请人： Kenneth G. Caldeira ,  Alistair K. Chan ,  Daniel B. Cook ,  William David Duncan ,  Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Jordin T. Kare ,  John Latham ,  Stephen H. Salter ,  Clarence T. Tegreene ,  David B. Tuckerman ,  Thomas A. Weaver ,  Charles Whitmer ,  Victoria Y. H. Wood ,  Lowell L. Wood, Jr.

发明人： Kenneth G. Caldeira ,  Alistair K. Chan ,  Daniel B. Cook ,  William David Duncan ,  Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Jordin T. Kare ,  John Latham ,  Stephen H. Salter ,  Clarence T. Tegreene ,  David B. Tuckerman ,  Thomas A. Weaver ,  Charles Whitmer ,  Victoria Y. H. Wood ,  Lowell L. Wood, Jr.

IPC分类号： B60L1/00 ,  B60L11/18 ,  H02J7/00

CPC分类号： B60L11/1801 ,  B60L11/18 ,  B60L11/1868 ,  B60L2200/12 ,  B60L2200/26 ,  B60L2200/28 ,  B60L2200/40 ,  H02J7/0054 ,  Y02P90/60 ,  Y02T10/7005 ,  Y02T10/7066

摘要： The present invention may include a towable trailer being mechanically couplable to a vehicle, the towable trailer including an auxiliary battery housing unit, a wheel being rotatably connected to the towable trailer, an auxiliary battery disposed within the auxiliary battery housing unit of the towable trailer, the vehicle including a storage battery, the auxiliary battery of the towable trailer being electrically couplable with the storage battery of the vehicle via a bidirectional pathway, and a control system configured to control a transfer of electrical energy between the auxiliary battery of the towable trailer and the storage battery of the vehicle along the bidirectional pathway.

摘要翻译： 本发明可以包括可车辆连接的拖车拖车，该牵引拖车包括一个辅助蓄电池壳体单元，一个可转动地连接到该拖车上的轮子，一个设置在该拖车拖车的辅助蓄电池容纳单元内的辅助蓄电池， 所述车辆包括蓄电池，所述拖曳拖车的辅助电池经由双向路径与所述车辆的蓄电池电耦合;以及控制系统，被配置为控制所述可拖曳拖车的辅助电池和 车辆的蓄电池沿着双向路径。

公开(公告)号：US08563271B2

公开(公告)日：2013-10-22

申请号：US13592116

申请日：2012-08-22

申请人： David G. Winkler ,  Jiye Shi ,  John Latham

发明人： David G. Winkler ,  Jiye Shi ,  John Latham

IPC分类号： C12N15/00 ,  C12N15/09 ,  C12N15/13 ,  C12N15/63

CPC分类号： C07K16/22 ,  A61K47/643 ,  A61K47/646 ,  C07K14/475 ,  C07K14/51 ,  C07K16/18 ,  C07K2317/76 ,  G01N33/6854 ,  G01N2333/4704

摘要： Compositions and methods relating to antibodies that specifically bind to TGF-beta binding proteins are provided. These methods and compositions relate to altering bone mineral density by interfering with the interaction between a TGF-beta binding protein sclerostin and a TGF-beta superfamily member, particularly a bone morphogenic protein. Increasing bone mineral density has uses in diseases and conditions in which low bone mineral density typifies the condition, such as osteopenia, osteoporosis, and bone fractures.

公开(公告)号：US20130257145A1

公开(公告)日：2013-10-03

申请号：US13436316

申请日：2012-03-30

申请人： Kenneth G. Caldeira ,  Alistair K. Chan ,  Daniel B. Cook ,  William David Duncan ,  Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Jordin T. Kare ,  John Latham ,  Stephen H. Salter ,  Clarence T. Tegreene ,  David B. Tuckerman ,  Thomas A. Weaver ,  Charles Whitmer ,  Victoria Y.H. Wood ,  Lowell L. Wood, JR.

发明人： Kenneth G. Caldeira ,  Alistair K. Chan ,  Daniel B. Cook ,  William David Duncan ,  Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Jordin T. Kare ,  John Latham ,  Stephen H. Salter ,  Clarence T. Tegreene ,  David B. Tuckerman ,  Thomas A. Weaver ,  Charles Whitmer ,  Victoria Y.H. Wood ,  Lowell L. Wood, JR.

IPC分类号： B60L1/00

CPC分类号： B60L11/1809 ,  B60D1/64 ,  B60L3/0046 ,  B60L7/18 ,  B60L11/123 ,  B60L11/126 ,  B60L11/14 ,  B60L11/1816 ,  B60L11/1861 ,  B60L11/1864 ,  B60L2200/12 ,  B60L2200/18 ,  B60L2200/26 ,  B60L2200/28 ,  B60L2200/40 ,  B60L2240/545 ,  B60L2240/622 ,  B60L2250/16 ,  B60L2270/40 ,  B60Y2200/91 ,  B60Y2200/92 ,  H02J7/0054 ,  Y02P90/60 ,  Y02T10/6217 ,  Y02T10/7005 ,  Y02T10/7044 ,  Y02T10/7061 ,  Y02T10/7072 ,  Y02T10/7077 ,  Y02T10/7291 ,  Y02T90/14 ,  Y02T90/16 ,  Y02T90/162 ,  Y10S903/903

摘要： The present invention may include a towable trailer being mechanically couplable to a vehicle, the towable trailer including an auxiliary battery housing unit, a wheel being rotatably connected to the towable trailer, an auxiliary battery disposed within the auxiliary battery housing unit of the towable trailer, the vehicle including a storage battery, the auxiliary battery of the towable trailer being electrically couplable with the storage battery of the vehicle via a bidirectional pathway, and a control system configured to control a transfer of electrical energy between the auxiliary battery of the towable trailer and the storage battery of the vehicle along the bidirectional pathway.

公开(公告)号：US20130203965A1

公开(公告)日：2013-08-08

申请号：US13741887

申请日：2013-01-15

申请人： John Latham ,  David G. Winkler

发明人： John Latham ,  David G. Winkler

IPC分类号： C07K16/22

CPC分类号： C07K16/22 ,  A61K39/3955 ,  A61K45/06 ,  A61K47/60 ,  A61K2039/505 ,  C07K14/76 ,  C07K14/79 ,  C07K16/18 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/30

摘要： Compositions and methods relating to epitopes of sclerostin protein, and sclerostin binding agents, such as antibodies capable of binding to sclerostin, are provided.

摘要翻译： 提供了与硬骨素蛋白表位有关的组合物和方法，以及硬骨素结合剂，例如能够结合硬化蛋白的抗体。

公开(公告)号：US20130017575A1

公开(公告)日：2013-01-17

申请号：US13298407

申请日：2011-11-17

申请人： Leon Garcia-Martinez ,  Anne Elisabeth Carvalho Jensen ,  Katie Olson ,  Ben Dutzar ,  Ethan Ojala ,  Brian Kovacevich ,  John Latham ,  Jeffrey T.L. Smith

发明人： Leon Garcia-Martinez ,  Anne Elisabeth Carvalho Jensen ,  Katie Olson ,  Ben Dutzar ,  Ethan Ojala ,  Brian Kovacevich ,  John Latham ,  Jeffrey T.L. Smith

IPC分类号： C12N15/13 ,  C12N15/63 ,  C12N1/19 ,  C12N7/01 ,  C12P21/02 ,  C12N1/21 ,  C12N15/62 ,  C12N5/10

CPC分类号： C07K16/248 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C12N9/60 ,  C12N9/64 ,  C12N15/81 ,  C12P21/02

摘要： The invention relates to nucleic acids encoding antibody or antibody fragments that specifically binds to an epitope on an intact human IL-6 polypeptide, which epitope when ascertained by epitopic mapping includes one or more residues comprised in each of the following IL-6 fragments (i) a fragment consisting of amino acid residues 37-51 of human Il-6, (ii) a fragment consisting of amino acid residues 70-84 of human Il-6, (iii) a fragment consisting of amino acid residues 169-183 of human Il-6, (iv) a fragment consisting of amino acid residues 31-45 of human Il-6 and (v) a fragment consisting of amino acid residues 58-72 of human IL-6.

摘要翻译： 本发明涉及编码与完整人IL-6多肽特异性结合表位的抗体或抗体片段的核酸，当通过表位作图确定时，该表位包括一个或多个残基，其包含在以下IL-6片段（i ）由人类II-6的氨基酸残基37-51组成的片段，（ii）由人类II-6的氨基酸残基70-84组成的片段，（iii）由氨基酸残基169-183 人II-6，（iv）由人IL-6的氨基酸残基31-45组成的片段和（v）由人IL-6的氨基酸残基58-72组成的片段。

公开(公告)号：US20120115240A1

公开(公告)日：2012-05-10

申请号：US12927315

申请日：2010-11-10

申请人： Kenneth G. Caldeira ,  Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Jordin T. Kare ,  John Latham ,  Nathan P. Myhrvold ,  Stephen H. Salter ,  Clarence T. Tegreene ,  David B. Tuckerman ,  Thomas Allan Weaver ,  Charles Whitmer ,  Lowell L. Wood, JR. ,  Victoria Y.H. Wood, JR.

发明人： Kenneth G. Caldeira ,  Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Jordin T. Kare ,  John Latham ,  Nathan P. Myhrvold ,  Stephen H. Salter ,  Clarence T. Tegreene ,  David B. Tuckerman ,  Thomas Allan Weaver ,  Charles Whitmer ,  Lowell L. Wood, JR. ,  Victoria Y.H. Wood, JR.

IPC分类号： G01N33/00 ,  B01J19/00 ,  C07C31/02 ,  G01N1/22

CPC分类号： B01D53/72 ,  A61B5/0022 ,  A61B5/082 ,  A61B5/1112 ,  A61B5/6819 ,  A61B2503/40 ,  B01J12/007 ,  B01J2219/00063 ,  B01J2219/00186 ,  B01J2219/00198 ,  B01J2219/00202 ,  B01J2219/00213 ,  B01J2219/00231 ,  B01J2219/00238 ,  B01J2219/1943 ,  Y10T436/203332 ,  Y10T436/214

摘要： Methane gas in a ruminant exhalation may be oxidized to reduce the amount of methane gas output by the ruminant.

摘要翻译： 反刍动物呼气中的甲烷气体可能被氧化，以减少反刍动物输出的甲烷气体的量。

公开(公告)号：US20110217303A1

公开(公告)日：2011-09-08

申请号：US13046860

申请日：2011-03-14

申请人： Jeffrey T.L. Smith ,  Randall C. Schatzman ,  Mark J. Litton ,  John Latham

发明人： Jeffrey T.L. Smith ,  Randall C. Schatzman ,  Mark J. Litton ,  John Latham

IPC分类号： A61K39/395 ,  A61P3/00 ,  A61P21/00

CPC分类号： C07K16/248 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2317/94

摘要： The present invention is directed to therapeutic methods using antibodies and fragments thereof having binding specificity for IL-6 to prevent or treat cachexia, fever, weakness and/or fatigue in a patient in need thereof. In preferred embodiments these patients will comprise those exhibiting (or at risk of developing) an elevated serum C-reactive protein level. In another preferred embodiment, the patient's survivability or quality of life will preferably be improved.

摘要翻译： 本发明涉及使用具有IL-6结合特异性的抗体及其片段以预防或治疗有需要的患者的恶病质，发热，乏力和/或疲劳的治疗方法。 在优选的实施方案中，这些患者将包括显示升高的血清C-反应蛋白水平（或具有发展风险）的那些。 在另一个优选实施例中，优选地改善患者的生存性或生活质量。

公开(公告)号：US07935340B2

公开(公告)日：2011-05-03

申请号：US12153612

申请日：2008-05-21

申请人： Leon Garcia-Martinez ,  Anne Elisabeth Carvalho Jensen ,  Katie Olson ,  Ben Dutzar ,  Ethan Ojala ,  Brian Kovacevich ,  John Latham ,  Jeffrey T. L. Smith

发明人： Leon Garcia-Martinez ,  Anne Elisabeth Carvalho Jensen ,  Katie Olson ,  Ben Dutzar ,  Ethan Ojala ,  Brian Kovacevich ,  John Latham ,  Jeffrey T. L. Smith

IPC分类号： A61K39/395 ,  C07K16/24 ,  C12P21/08

CPC分类号： C07K16/248 ,  A61K39/3955 ,  A61K45/06 ,  A61K2039/505 ,  C07K2317/20 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/41 ,  C07K2317/76 ,  C07K2317/94

摘要： The present invention is directed to antibodies and fragments thereof having binding specificity for IL-6. Another embodiment of this invention relates to the antibodies described herein, and binding fragments thereof, comprising the sequences of the VH, VL and CDR polypeptides described herein, and the polynucleotides encoding them. The invention also contemplates conjugates of anti-IL-6 antibodies and binding fragments thereof conjugated to one or more functional or detectable moieties. The invention also contemplates methods of making said anti-IL-6 antibodies and binding fragments thereof. Embodiments of the invention also pertain to the use of anti-IL-6 antibodies, and binding fragments thereof, for the diagnosis, assessment and treatment of diseases and disorders associated with IL-6. These antibodies may bind at least one of soluble IL-6, cell surface expressed IL-6, IL-6/IL-6R and/or prevent the association of IL-6 and IL-6R, the association of IL-6/IL-6R and gp130 and or the formation of IL-6/IL-6R/gp130 multimers and thereby inhibit a biological effect associated with any of the foregoing.

摘要翻译： 本发明涉及对IL-6具有结合特异性的抗体及其片段。 本发明的另一个实施方案涉及本文所述的抗体及其结合片段，其包含本文所述的VH，VL和CDR多肽的序列，以及编码它们的多核苷酸。 本发明还涉及抗IL-6抗体及其与一个或多个功能或可检测部分缀合的结合片段的缀合物。 本发明还考虑制备所述抗IL-6抗体及其结合片段的方法。 本发明的实施方案还涉及抗IL-6抗体及其结合片段用于诊断，评估和治疗与IL-6相关的疾病和病症的用途。 这些抗体可以结合可溶性IL-6，细胞表面表达的IL-6，IL-6 / IL-6R中的至少一种和/或阻止IL-6和IL-6R的结合，IL-6 / IL -6R和gp130，或者形成IL-6 / IL-6R / gp130多聚体，从而抑制与前述任何相关的生物学作用。

公开(公告)号：US20100150829A1

公开(公告)日：2010-06-17

申请号：US12624778

申请日：2009-11-24

申请人： Leon Garcia-Martinez ,  Anne Elisabeth Carvalho Jensen ,  Katie Olson ,  Ben Dutzar ,  John Latham ,  Brian Kovacevich ,  Jeffrey T.L. Smith ,  Mark Litton ,  Randall Schatzman

发明人： Leon Garcia-Martinez ,  Anne Elisabeth Carvalho Jensen ,  Katie Olson ,  Ben Dutzar ,  John Latham ,  Brian Kovacevich ,  Jeffrey T.L. Smith ,  Mark Litton ,  Randall Schatzman

IPC分类号： A61K51/00 ,  G01N33/53 ,  A61K39/395 ,  A61K49/00

CPC分类号： A61K51/1033 ,  A61K49/0058 ,  A61K2039/505 ,  C07K16/248 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/6869 ,  G01N2333/5412 ,  G01N2800/7095 ,  Y02A50/412

摘要： The present invention is directed to therapeutic methods using IL-6 antagonists such as an Ab1 antibody or antibody fragment having binding specificity for IL-6 to prevent or treat disease or to improve survivability or quality of life of a patient in need thereof. In preferred embodiments these patients will comprise those exhibiting (or at risk of developing) an elevated serum C-reactive protein level, reduced serum albumin level, elevated D-dimer or other coagulation cascade related protein(s), cachexia, fever, weakness and/or fatigue prior to treatment. The subject therapies also may include the administration of other actives such as chemotherapeutics, anti-coagulants, statins, and others.

摘要翻译： 本发明涉及使用IL-6拮抗剂例如Ab1抗体或对IL-6具有结合特异性的抗体片段来预防或治疗疾病或改善有需要的患者的生存能力或生活质量的治疗方法。 在优选实施方案中，这些患者将包括显示（或有发展风险）升高的血清C-反应蛋白水平，降低的血清白蛋白水平，升高的D-二聚体或其他凝血级联相关蛋白，恶病质，发热，虚弱和 /或治疗前的疲劳。 主题疗法还可以包括施用其它活性物质，例如化学治疗剂，抗凝血剂，他汀类药物等。