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    Shortened purification process for the production of capsular Streptococcus pneumoniae polysaccharides
    1.
    发明授权
    Shortened purification process for the production of capsular Streptococcus pneumoniae polysaccharides 有权
    缩短生产荚膜肺炎链球菌多糖的纯化方法

    公开(公告)号：US08652480B2

    公开(公告)日：2014-02-18

    申请号：US12052525

    申请日：2008-03-20

    申请人： Yonghui Yuan ,  Mark Ruppen ,  Wei-Qiang Sun ,  Ling Chu ,  John Simpson ,  James Patch ,  Justin Keith Moran ,  Pamela Fink

    发明人： Yonghui Yuan ,  Mark Ruppen ,  Wei-Qiang Sun ,  Ling Chu ,  John Simpson ,  James Patch ,  Justin Keith Moran ,  Pamela Fink

    IPC分类号： A61K31/70 ,  A61K39/00

    CPC分类号： A61K39/092 ,  A61K39/00 ,  A61K47/646 ,  A61K2039/6087 ,  A61K2039/62 ,  C07H1/08 ,  C08B37/00 ,  C08B37/0003 ,  C12P19/04

    摘要： A shortened process for producing a solution containing substantially purified capsular polysaccharides from a cellular Streptococcus pneumoniae lysate broth is described. Ultrafiltering and diafiltering a clarified S. pneumoniae lysate followed by pH adjustment to less than 4.5, preferably about 3.5, precipitated at least 98% of the protein in the solution without seriously affecting polysaccharide yield. Furthermore, following ultrafiltration and diafiltration and acidification to a pH of less than 4.5, filtration using activated carbon precipitated at least 90% of remaining protein without seriously affecting polysaccharide yield. Exemplary, non-limiting S. pneumoniae serotypes that can be purified using the shortened process of the invention are 1, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F. In one embodiment, the Streptococcus pneumoniae cells are lysed using deoxycholate sodium (DOC), while in another embodiment the lytic agent is a non-animal derived lytic agent such as N-lauryl sarcosine sodium (NLS).

    摘要翻译： 描述了从细胞性肺炎链球菌裂解液肉汤制备含有基本上纯化的荚膜多糖的溶液的缩短方法。 超滤并透析澄清的肺炎链球菌裂解物，然后pH调节至小于4.5，优选约3.5，在溶液中沉淀至少98％的蛋白质，而不会严重影响多糖产量。 此外，在超滤和渗滤并酸化至pH小于4.5之后，使用活性炭过滤沉淀至少90％的剩余蛋白质而不严重影响多糖产率。 可以使用本发明的缩短方法纯化的示例性非限制性肺炎链球菌血清型是1,4,5,6A，6B，7F，9V，14,18C，19A，19F和23F。 在一个实施方案中，使用脱氧胆酸钠（DOC）裂解肺炎链球菌细胞，而在另一个实施方案中，溶解剂是非动物来源的溶解剂，例如N-月桂基肌氨酸钠（NLS）。