公开(公告)号：US5942093A

公开(公告)日：1999-08-24

申请号：US878470

申请日：1997-06-18

Applicant: David J. Rakestraw ,  Deon S. Anex ,  Chao Yan ,  Rajeev Dadoo ,  Richard N. Zare

Inventor： David J. Rakestraw ,  Deon S. Anex ,  Chao Yan ,  Rajeev Dadoo ,  Richard N. Zare

IPC: G01N27/447 ,  G01N30/28 ,  G01N27/00

CPC classification number: G01N27/44704 ,  G01N2030/285

Abstract: Method and apparatus for controlling precisely the composition and delivery of liquid at sub-.mu.L/min flow rate. One embodiment of such a delivery system is an electro-osmotically driven gradient flow delivery system that generates dynamic gradient flows with sub-.mu.L/min flow rates by merging a plurality of electro-osmotic flows. These flows are delivered by a plurality of delivery arms attached to a mixing connector, where they mix and then flow into a receiving means, preferably a column. Each inlet of the plurality of delivery arms is placed in a corresponding solution reservoir. A plurality of independent programmable high-voltage power supplies is used to apply a voltage program to each of the plurality of solution reservoirs to regulate the electro-osmotic flow in each delivery arm. The electro-osmotic flow rates in the delivery arms are changed with time according to each voltage program to deliver the required gradient profile to the column.

Abstract translation: 用于以低于L / min流速精确控制液体的组成和输送的方法和装置。 这种输送系统的一个实施例是电渗透驱动的梯度流输送系统，其通过合并多个电渗流而产生具有亚L / min流速的动态梯度流。 这些流动由连接到混合连接器的多个输送臂输送，在该混合连接器中它们混合，然后流入接收装置，优选塔。 多个输送臂的每个入口被放置在相应的溶液储存器中。 多个独立的可编程高压电源用于将多个溶液储存器中的每一个施加电压程序以调节每个输送臂中的电渗流。 输送臂中的电渗流速度根据每个电压程序随时间而改变，以将所需的梯度曲线递送到柱。

公开(公告)号：US5310463A

公开(公告)日：1994-05-10

申请号：US975850

申请日：1992-11-13

Applicant: Rajeev Dadoo ,  Luis A. Colon ,  Harvey A. Fishman ,  Jason B. Shear ,  Richard N. Zare

Inventor： Rajeev Dadoo ,  Luis A. Colon ,  Harvey A. Fishman ,  Jason B. Shear ,  Richard N. Zare

IPC: G01N30/02 ,  G01N30/16 ,  G01N30/28 ,  G01N30/60 ,  G01N30/84 ,  G01N27/26 ,  G01N27/447

CPC classification number: G01N30/606 ,  G01N30/16 ,  G01N30/6039 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/8435 ,  G01N30/02 ,  G01N30/6034

Abstract: An electrophoretic or chromatographic separation capillary containing a fluid defines a bore therein through which a sample travels and separates into components. The tube has a side wall defining a through hole therein which is surrounded by a medium including a substance. The substance is introduced into the capillary through the hole by means of gravity, pressure or electroosmosis. The substance introduced may be used to label sample components to enhance detection, or to enhance separation of sample components.

Abstract translation: 含有流体的电泳或色谱分离毛细管限定了一个孔，样品通过该孔穿过并分离成组分。 管具有限定其中的通孔的侧壁，该通孔被包括物质的介质包围。 物质通过重力，压力或电沉积通过孔引入毛细管中。 引入的物质可用于标记样品组分以增强检测，或增强样品组分的分离。

公开(公告)号：US5480525A

公开(公告)日：1996-01-02

申请号：US235299

申请日：1994-04-29

Applicant: Luis A. Colon ,  Rajeev Dadoo ,  William H. Whitted ,  Richard N. Zare ,  Andrew G. Ewing ,  Sandra S. Ferris ,  Jennifer U. Woelker

Inventor： Luis A. Colon ,  Rajeev Dadoo ,  William H. Whitted ,  Richard N. Zare ,  Andrew G. Ewing ,  Sandra S. Ferris ,  Jennifer U. Woelker

IPC: G01N27/07 ,  G01N27/447 ,  B01D61/42 ,  B01D61/46

CPC classification number: G01N27/4473 ,  G01N27/44704 ,  G01N27/44721

Abstract: End-column detectors are provided in which either a sensing microelectrode or optical fiber is placed at the end of the separation capillary. Additionally, an alignment apparatus is provided to facilitate the manual or automated positioning of either type of sensing device relative to the end of the separation capillary. The invention provides detectors that are sensitive, reliable, and easy to construct and to operate.

Abstract translation: 提供端柱检测器，其中感测微电极或光纤放置在分离毛细管的末端。 此外，提供了一种对准装置以便于相对于分离毛细管的端部手动或自动定位任一类型的感测装置。 本发明提供了敏感，可靠，易于构造和操作的检测器。

公开(公告)号：US5378334A

公开(公告)日：1995-01-03

申请号：US158807

申请日：1993-11-24

Applicant: Rajeev Dadoo ,  Thomas T. Lee ,  Richard N. Zare

Inventor： Rajeev Dadoo ,  Thomas T. Lee ,  Richard N. Zare

IPC: G01N27/447 ,  G01N30/02 ,  G01N30/16 ,  G01N30/28 ,  G01N30/60 ,  G01N30/84 ,  G01N27/26

CPC classification number: G01N30/16 ,  G01N27/44717 ,  G01N27/44773 ,  G01N30/6039 ,  G01N2030/027 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/8435 ,  G01N30/02

Abstract: An electrophoretic or chromatographic separation capillary containing a fluid or gel defines a bore therein through which a sample migrates and separates into components. The tube has a side wall defining a passage therein. A substance is introduced into the capillary through the pasage by means of gravity, pressure or electroosmosis. The substance introduced changes the electroosmotic flow rate of the fluid in the capillary tube. The electroosmotic flow rate of the fluid in the capillary tube can be monitored by introducing a compound through the passage and detecting the concentration of the compound. Information on the flow rate can be used in a feedback action to control the flow rate, such as to keep it constant.

Abstract translation: 含有流体或凝胶的电泳或色谱分离毛细管在其中限定了孔，样品通过该孔移动并分离成组分。 管具有在其中限定通道的侧壁。 一种物质通过重力，压力或电渗渗透通过管道引入毛细管中。 所引入的物质改变了毛细管中流体的电渗流速。 可以通过引入化合物通过通道并检测化合物的浓度来监测毛细管中的流体的电渗流速。 关于流量的信息可以用于反馈动作以控制流量，例如保持其恒定。

公开(公告)号：US5503994A

公开(公告)日：1996-04-02

申请号：US134290

申请日：1993-10-08

Applicant: Jason B. Shear ,  Rajeev Dadoo ,  Harvey A. Fishman ,  Neil Shafer ,  Richard N. Zare

Inventor： Jason B. Shear ,  Rajeev Dadoo ,  Harvey A. Fishman ,  Neil Shafer ,  Richard N. Zare

IPC: G01N27/447 ,  G01N30/00 ,  G01N30/62 ,  G01N30/74 ,  G01N35/08 ,  G01N21/00 ,  C12Q1/68 ,  G01N21/64

CPC classification number: G01N27/44721 ,  G01N30/62 ,  G01N30/0005 ,  G01N30/74 ,  G01N35/085 ,  Y10T436/118339

Abstract: In many separation techniques, such as field flow fractionation, liquid chromatography and electro-phoresis, chemical species form bands that migrate at different velocities. If the data-digitization rate and excitation intensity are both set to be optimal for the fastest migrating band, to compensate for different band velocities, both the data-digitization rate and the excitation intensity are decreased as a function of time by a factor equal to the migration time of the fastest migrating band to the separation time.

Abstract translation: 在许多分离技术，如场流分馏，液相色谱和电泳法中，化学物质形成以不同速度迁移的条带。 如果数据数字化速率和激励强度都被设置为对于最快迁移频带是最佳的，为了补偿不同的频带速度，数据数字化速率和激励强度作为时间的函数被减少一个因子等于 最快迁移频段到分离时间的迁移时间。

公开(公告)号：US5342492A

公开(公告)日：1994-08-30

申请号：US115466

申请日：1993-09-01

Applicant: Rajeev Dadoo ,  Richard N. Zare ,  Luis A. Colon

Inventor： Rajeev Dadoo ,  Richard N. Zare ,  Luis A. Colon

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/4473 ,  G01N27/44773

Abstract: A voltage alternating between a high value and a low value is applied across a separation capillary in an electrophoretic system and electrochemical detection is performed only during time periods when a low voltage or no voltage is applied to the tube to improve the sensitivity of detection.

Abstract translation: 在电泳系统中跨越分离毛细管施加在高值和低值之间交替的电压，并且仅在对管施加低电压或无电压以提高检测灵敏度的时间段期间进行电化学检测。