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    HYBRID ALPHA-AMYLASES
    2.
    发明申请
    HYBRID ALPHA-AMYLASES 有权
    混合阿尔法胺

    公开(公告)号：US20120021485A1

    公开(公告)日：2012-01-26

    申请号：US13132881

    申请日：2009-12-11

    申请人： Scott D. Power ,  Andrew Shaw

    发明人： Scott D. Power ,  Andrew Shaw

    IPC分类号： C12N9/96 ,  G06G7/60 ,  C12N1/21 ,  C12N1/15 ,  C12N15/62 ,  C12N15/63

    CPC分类号： C12N9/2417 ,  C07K2319/00 ,  G06F19/12 ,  G06F19/16

    摘要： Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like ?-amylase, e.g., a Bacillus amylase. In the hybrid, an N terminal portion of a Termamyl-like ?-amylase is replaced with sequences from an archae ? amylase. The sequence similarity between the two amylase sequences may be less than 60%. Conserving the wild-type 3D structure in the hybrid facilitates obtaining enzymatically active amylases. In one embodiment, one or both amylase sequences contribute residues to the B domain, resulting in particularly advantageous properties. For instance, replacement of the Ca2+ binding site in the B domain of the Termamyl-like ?-amylase with a B domain sequence of an archae ? amylase that does not bind Ca2+ can produce a hybrid that is fully active in the absence of Ca2+.

    摘要翻译： 提供杂交α-淀粉酶，其与野生型类戊酰样α-淀粉酶（例如芽孢杆菌淀粉酶）全部或部分共享保守的3D结构。 在杂交体中，类戊酰样α-淀粉酶的N末端部分被来自古细菌的序列替代 淀粉酶。 两个淀粉酶序列之间的序列相似性可以小于60％。 在杂交中保护野生型3D结构有助于获得酶活性淀粉酶。 在一个实施方案中，一个或两个淀粉酶序列向B结构域贡献残基，导致特别有利的性质。 例如，用具有古细菌的B结构域序列的Termamyl样α-淀粉酶的B结构域中的Ca 2+结合位点的替换 不结合Ca2 +的淀粉酶可以产生在不存在Ca 2+的情况下完全活性的杂交体。

    Hybrid alpha-amylases
    4.
    发明授权
    Hybrid alpha-amylases 有权
    混合型α-淀粉酶

    公开(公告)号：US08841107B2

    公开(公告)日：2014-09-23

    申请号：US13132881

    申请日：2009-12-11

    申请人： Scott D. Power ,  Andrew Shaw

    发明人： Scott D. Power ,  Andrew Shaw

    IPC分类号： C12N9/96 ,  C12N9/00 ,  C12N9/28

    CPC分类号： C12N9/2417 ,  C07K2319/00 ,  G06F19/12 ,  G06F19/16

    摘要： Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like ?-amylase, e.g., a Bacillus amylase. In the hybrid, an N terminal portion of a Termamyl-like ?-amylase is replaced with sequences from an archae ? amylase. The sequence similarity between the two amylase sequences may be less than 60%. Conserving the wild-type 3D structure in the hybrid facilitates obtaining enzymatically active amylases. In one embodiment, one or both amylase sequences contribute residues to the B domain, resulting in particularly advantageous properties. For instance, replacement of the Ca2+ binding site in the B domain of the Termamyl-like ?-amylase with a B domain sequence of an archae ? amylase that does not bind Ca2+ can produce a hybrid that is fully active in the absence of Ca2+.

    摘要翻译： 提供杂交α-淀粉酶，其与野生型类戊酰样α-淀粉酶（例如芽孢杆菌淀粉酶）全部或部分共享保守的3D结构。 在杂交体中，类戊酰样α-淀粉酶的N末端部分被来自古细菌的序列替代 淀粉酶。 两个淀粉酶序列之间的序列相似性可以小于60％。 在杂交中保护野生型3D结构有助于获得酶活性淀粉酶。 在一个实施方案中，一个或两个淀粉酶序列向B结构域贡献残基，导致特别有利的性质。 例如，用具有古细菌的B结构域序列的Termamyl样α-淀粉酶的B结构域中的Ca 2+结合位点的替换 不结合Ca2 +的淀粉酶可以产生在不存在Ca 2+的情况下完全活性的杂交体。

    Alpha-amylase variants with altered properties
    5.
    发明授权
    Alpha-amylase variants with altered properties 有权
    具有改变性质的α-淀粉酶变体

    公开(公告)号：US07541026B2

    公开(公告)日：2009-06-02

    申请号：US12263631

    申请日：2008-11-03

    申请人： Scott D. Power ,  Sandra W. Ramer ,  Andrew Shaw

    发明人： Scott D. Power ,  Sandra W. Ramer ,  Andrew Shaw

    IPC分类号： A61K38/46 ,  C12N9/28

    CPC分类号： C11D3/38681 ,  C11D3/386 ,  C12N9/2417 ,  D06M16/003

    摘要： Disclosed are compositions comprising variants of a parent G. stearothermophilus alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

    摘要翻译： 公开了包含具有α-淀粉酶活性的亲代嗜热脂肪芽孢杆菌α-淀粉酶的变体的组合物，并且相对于从其衍生的亲本AmyS样α-淀粉酶表现出改变的性质。 组合物包含另外的酶如植酸酶。 还公开了使用组合物的方法和与其相关的试剂盒。

    ALPHA-AMYLASE VARIANTS WITH ALTERED PROPERTIES
    8.
    发明申请
    ALPHA-AMYLASE VARIANTS WITH ALTERED PROPERTIES 有权
    具有改性性质的ALPHA-AMYLASE变体

    公开(公告)号：US20090117642A1

    公开(公告)日：2009-05-07

    申请号：US12263631

    申请日：2008-11-03

    申请人： Scott D. Power ,  Sandra W. Ramer ,  Andrew Shaw

    发明人： Scott D. Power ,  Sandra W. Ramer ,  Andrew Shaw

    IPC分类号： C12N9/28

    CPC分类号： C11D3/38681 ,  C11D3/386 ,  C12N9/2417 ,  D06M16/003

    摘要： Disclosed are compositions comprising variants of a parent G. stearothermophilus alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

    摘要翻译： 公开了包含具有α-淀粉酶活性的亲代嗜热脂肪芽孢杆菌α-淀粉酶的变体的组合物，并且相对于从其衍生的亲本AmyS样α-淀粉酶表现出改变的性质。 组合物包含另外的酶如植酸酶。 还公开了使用组合物的方法和与其相关的试剂盒。