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    METHOD FOR FORMING A CELL AGGREGATE
    1.
    发明申请
    METHOD FOR FORMING A CELL AGGREGATE 审中-公开
    形成细胞聚集体的方法

    公开(公告)号：US20130266954A1

    公开(公告)日：2013-10-10

    申请号：US13993152

    申请日：2011-12-07

    申请人： Shigeyoshi Fujiwara ,  Keigo Takei ,  Yukimitsu Suda

    发明人： Shigeyoshi Fujiwara ,  Keigo Takei ,  Yukimitsu Suda

    IPC分类号： G01N33/50 ,  C12N5/071 ,  C12N5/09 ,  C12M1/42

    CPC分类号： G01N33/502 ,  C12M23/20 ,  C12M35/08 ,  C12N5/0068 ,  C12N5/0667 ,  C12N5/0671 ,  C12N5/0693 ,  C12N2533/30 ,  G01N33/5032 ,  G01N33/5082

    摘要： A method for forming a cell aggregate in the present invention is a method for forming a cell aggregate by using a culturing vessel and culturing an adhesive cell, wherein the culturing vessel is such that an amino group, a carboxyl group, and/or a hydroxyl group, and a group represented by a general formula of (in the formula, each of R1, R2, and R3 is independently an alkyl group with a carbon number greater than or equal to 1 and less than or equal to 6 and m is an integer greater than or equal to 2 and less than or equal to 6.) are present near a surface thereof and the adhesive cell is a mesenchymal stem cell, a hepatic cell, or a cancer cell.

    摘要翻译： 本发明的细胞集合体的形成方法是使用培养容器形成细胞集合体并培养粘合细胞的方法，其中培养容器为氨基，羧基和/或羟基 （通式中，R 1，R 2和R 3各自独立地为碳数大于等于1且小于等于6的烷基，m为 大于或等于2且小于或等于6的整数）存在于其表面附近，并且粘合剂细胞是间充质干细胞，肝细胞或癌细胞。

    Insoluble powder, powder for restoring barrier function of skin, powder for preventing/mitigating skin chapping, and preparation containing the same for external use on skin
    6.
    发明授权
    Insoluble powder, powder for restoring barrier function of skin, powder for preventing/mitigating skin chapping, and preparation containing the same for external use on skin 有权
    不溶性粉末，用于恢复皮肤屏障功能的粉末，用于预防/缓解皮肤裂纹的粉末，以及含有外用皮肤的制剂

    公开(公告)号：US07785636B2

    公开(公告)日：2010-08-31

    申请号：US10509539

    申请日：2003-03-28

    申请人： Shigeyoshi Fujiwara ,  Mitsuhiro Denda ,  Katsuki Ogawa ,  Tsuyoshi Miyamoto ,  Sadaki Takata

    发明人： Shigeyoshi Fujiwara ,  Mitsuhiro Denda ,  Katsuki Ogawa ,  Tsuyoshi Miyamoto ,  Sadaki Takata

    IPC分类号： A61K33/04

    CPC分类号： A61Q1/12 ,  A61K8/022 ,  A61K8/19 ,  A61K8/23 ,  A61K8/27 ,  A61K2800/412 ,  A61K2800/651 ,  A61Q1/02 ,  A61Q1/06 ,  A61Q1/10 ,  A61Q17/00 ,  A61Q19/00 ,  A61Q19/08

    摘要： A first subject of the present invention is an insoluble powder characterized in that a zeta-potential is a negative value.It is preferable that barium sulfate is a main ingredient in the insoluble powder.It is preferable that an average primary particle diameter is 3 to 100 μm, and an aspect ratio is 3 to 250 in the insoluble powder.It is preferable that the insoluble powder is a metal-doped barium sulfate powder obtained by reacting a barium ion and a sulfate ion in the presence of a metal ion.It is preferable that the metal ion is one or more selected from the group consisting of a lithium ion, a sodium ion and a zinc ion in the insoluble powder.A second subject of the present invention is a skin barrier function recovering powder, or a skin roughening preventing and improving powder, which comprises the aforementioned powder.A third subject of the present invention is a skin external composition characterized in that a content of the aforementioned powder is 1 to 40% by weight.

    摘要翻译： 本发明的第一个目标是不溶性粉末，其特征在于ζ电位是负值。 优选硫酸钡是不溶性粉末的主要成分。 在不溶性粉末中，平均一次粒径优选为3〜100μm，长径比为3〜250。 优选不溶性粉末是在金属离子存在下使钡离子和硫酸根离子反应而得到的金属掺杂硫酸钡粉末。 金属离子优选在不溶性粉末中选自锂离子，钠离子和锌离子中的一种以上。 本发明的第二主题是包含上述粉末的皮肤屏障功能回收粉末或皮肤粗糙化防止和改善粉末。 本发明的第三主题是皮肤外用组合物，其特征在于上述粉末的含量为1〜40重量％。

    Primitive organ-like structure comprising keratinocytes and hair papilla cells
    7.
    发明授权
    Primitive organ-like structure comprising keratinocytes and hair papilla cells 有权
    包含角质形成细胞和毛发乳头细胞的原始器官样结构

    公开(公告)号：US08563309B2

    公开(公告)日：2013-10-22

    申请号：US12306649

    申请日：2007-06-27

    申请人： Shigeyoshi Fujiwara ,  Jiro Kishimoto ,  Tsutomu Soma

    发明人： Shigeyoshi Fujiwara ,  Jiro Kishimoto ,  Tsutomu Soma

    IPC分类号： C12N15/02

    CPC分类号： C12N5/0697 ,  C12N5/0625 ,  C12N5/0627 ,  C12N5/0629 ,  C12N5/0666 ,  C12N2501/40 ,  C12N2501/415

    摘要： The present invention provides a method of producing a cell mass capable of serving as a primitive organ-like structure comprised of a plurality of somatic cell types of somatic origin, comprising: preparing cultures containing the plurality of types of somatic cells; mixing the plurality of types of somatic cell cultures followed by adding a Wnt signal activator to the mixed cell culture; subjecting the culture containing the Wnt signal activator to non-plate contact culturing over a predetermined time period; and replacing the medium of the culture cultured by the non-plate contact culturing with medium not containing Wnt signal activator and further culturing for a predetermined time period; wherein, at least one type of the plurality of somatic cells is maintained in an undifferentiated state.

    摘要翻译： 本发明提供一种制备能够作为由体细胞起源的多种体细胞类型构成的原始器官状结构的细胞块的方法，其包括：制备含有多种体细胞的培养物; 混合多种类型的体细胞培养物，然后向混合细胞培养物中加入Wnt信号激活剂; 使含有Wnt信号激活剂的培养物在预定时间段内进行非平板接触培养; 并用不含Wnt信号激活剂的培养基替换由非平板接触培养培养的培养基，并进一步培养预定时间; 其中，至少一种类型的所述多个体细胞维持在未分化状态。