公开(公告)号：US20090137034A1

公开(公告)日：2009-05-28

申请号：US12276652

申请日：2008-11-24

Applicant: John K. Leach ,  Steven R. Tannenbaum

Inventor： John K. Leach ,  Steven R. Tannenbaum

IPC: C12N5/02

CPC classification number: G01N33/5005 ,  C12N5/067 ,  C12N2500/38 ,  C12N2501/999 ,  C12N2503/00

Abstract: The present invention provides methods for culturing primary hepatocytes with improved long term function and improved viability, by plating the hepatocytes in the presence of an anti-oxidant(s) as well as an agent(s) which is a functional inhibitor of enzymes that generate reactive oxygen and reactive nitrogen species. One preferred embodiment provides a combination of 2-oxo-thizolidine and tocopherol succinate. Another preferred embodiment provides a combination of NG-methylarginine and mannitol.

Abstract translation: 本发明提供了通过在抗氧化剂存在下电镀肝细胞以及作为酶的功能抑制剂的药剂来培养具有改善的长期功能和改善的存活力的原代肝细胞的方法， 活性氧和活性氮。 一个优选的实施方案提供2-氧代 - 噻唑烷和琥珀酸生育酚的组合。 另一个优选实施方案提供了NG-甲基精氨酸和甘露糖醇的组合。

公开(公告)号：US08318479B2

公开(公告)日：2012-11-27

申请号：US11133092

申请日：2005-05-19

Applicant: Karel Domansky ,  Linda G. Griffith ,  Steven R. Tannenbaum ,  Alan Wells ,  Samuel Walker Inman

Inventor： Karel Domansky ,  Linda G. Griffith ,  Steven R. Tannenbaum ,  Alan Wells ,  Samuel Walker Inman

IPC: C12M1/22 ,  C12M3/00 ,  C12M1/34

CPC classification number: B01L3/502715 ,  B01J2219/00315 ,  B01J2219/00743 ,  B01L3/50255 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/0481 ,  B01L2400/0638 ,  B33Y80/00 ,  C12M23/12 ,  C12M23/40 ,  C12M25/14 ,  C12M29/10

Abstract: A system has been constructed that recapitulate the features of a capillary bed through normal human tissue. The system facilitates perfusion of three-dimensional (3D) cell monocultures and heterotypic cell co-cultures at the length scale of the capillary bed. A major feature is that the system can be utilized within a “multiwell plate” format amenable to high-throughput assays compatible with the type of robotics commonly used in pharmaceutical development. The system provides a means to conduct assays for toxicology and metabolism and as a model for human diseases such as hepatic diseases, including hepatitis, exposure-related pathologies, and cancer. Cancer applications include primary liver cancer as well as metastases. The system can also be used as a means of testing gene therapy approaches for treating disease and inborn genetic defects.

Abstract translation: 已经构建了通过正常人体组织重现毛细血管床特征的系统。 该系统有助于在毛细血管床的长度尺度上灌注三维（3D）细胞单一培养物和异型细胞共培养物。 主要特征是该系统可以在多孔板格式中使用，适合与药物开发中通常使用的机器人类型兼容的高通量测定。 该系统提供了进行毒理学和代谢测定的手段，并作为人类疾病（如肝脏疾病，包括肝炎，暴露相关病理和癌症）的模型。 癌症应用包括原发性肝癌以及转移瘤。 该系统还可用作测试基因治疗方法的手段，用于治疗疾病和先天性遗传缺陷。

公开(公告)号：US06867415B2

公开(公告)日：2005-03-15

申请号：US09938277

申请日：2001-08-23

Applicant: Barbara J. Hughey ,  Paul L. Skipper ,  John S. Wishnok ,  Ruth E. Shefer ,  Naomi A. Fried ,  John T. Mehl ,  Steven R. Tannenbaum

Inventor： Barbara J. Hughey ,  Paul L. Skipper ,  John S. Wishnok ,  Ruth E. Shefer ,  Naomi A. Fried ,  John T. Mehl ,  Steven R. Tannenbaum

IPC: G01N30/72 ,  G01N30/84 ,  H01J49/04 ,  H01J49/26

CPC classification number: H01J49/0463 ,  G01N30/7233 ,  G01N2030/8405 ,  H01J49/0445

Abstract: An interface for introducing a non-gaseous sample as a predetermined gaseous form into an accelerator mass spectrometer which comprises a nebulizer that receives the non-gaseous sample to provide a fine spray of the sample, a converter that receives at least a portion of said fine spray and converts the desired elements to the predetermined gaseous form and a flow line that transports the predetermined gaseous form to the accelerator mass spectrometer.

Abstract translation: 用于将非气态样品作为预定气体形式引入加速器质谱仪的界面，该加速器质谱仪包括接收非气态样品以提供样品的细微喷雾的雾化器，接收所述精细的至少一部分的转化器 喷雾并将期望的元素转化成预定的气体形式和将预定气体形式输送到加速器质谱仪的流动管线。

公开(公告)号：US3968009A

公开(公告)日：1976-07-06

申请号：US578772

申请日：1975-05-19

Applicant: Steven R. Tannenbaum ,  Anthony J. Sinskey ,  Stephen B. Maul

Inventor： Steven R. Tannenbaum ,  Anthony J. Sinskey ,  Stephen B. Maul

IPC: C12N1/08 ,  C12C11/00

CPC classification number: C12N1/08 ,  Y10S435/839 ,  Y10S435/921 ,  Y10S435/923 ,  Y10S435/942

Abstract: Process for reducing nucleic acid content of yeasts and bacteria comprising heat shocking for a period of time and at a temperature selected to increase effectiveness of enzymes which break down nucleic acids without allowing proteins to break down or leak out of the cell, and incubating the heat-shocked cells at a temperature lower than the heat shock temperature under conditions in which the cells remain intact and nucleic acid fragments permeate the cell walls.

Abstract translation: 用于降低酵母和细菌的核酸含量的方法，所述酵母和细菌包括热休克一段时间和选定的温度，以增加分解核酸而不允许蛋白质分解或泄漏出细胞的酶的有效性，并孵育热量 在保持细胞保持完整且核酸碎片渗透细胞壁的条件下，在低于热休克温度的温度下进行沉积。

公开(公告)号：US5139933A

公开(公告)日：1992-08-18

申请号：US542695

申请日：1990-06-25

Applicant: Calvert L. Green ,  Franz Fiedler ,  Thomsen J. Hansen ,  Gerald N. Wogan ,  Steven R. Tannenbaum ,  Thomas L. Benjamin

Inventor： Calvert L. Green ,  Franz Fiedler ,  Thomsen J. Hansen ,  Gerald N. Wogan ,  Steven R. Tannenbaum ,  Thomas L. Benjamin

IPC: G01N33/53 ,  G01N33/569 ,  G01N33/577 ,  G01N33/66 ,  G01N33/68

CPC classification number: G01N33/66 ,  G01N33/5308 ,  G01N33/56911 ,  G01N33/68 ,  Y10S435/82 ,  Y10S435/961

Abstract: An assay method is provided to quickly detect the presence of Listeria strains in samples, characterized by the use of antibodies to selectively capture the peptidoglycan and teichoic acid components of the listeriae bacterial cell wall.

公开(公告)号：US06707035B2

公开(公告)日：2004-03-16

申请号：US09935909

申请日：2001-08-23

Applicant: Barbara J. Hughey ,  Paul L. Skipper ,  John S. Wishnok ,  Ruth E. Shefer ,  John T. Mehl ,  Steven R. Tannenbaum

Inventor： Barbara J. Hughey ,  Paul L. Skipper ,  John S. Wishnok ,  Ruth E. Shefer ,  John T. Mehl ,  Steven R. Tannenbaum

IPC: H01J4926

CPC classification number: H01J49/0463 ,  G01N30/7233 ,  G01N2030/8405 ,  H01J49/0445

Abstract: An interface system for introducing a non-gaseous sample as a predetermined gaseous form into an analytical instrument which comprises a substrate that receives the non-gaseous sample and a directed energy source that heats at least a portion of said substrate containing the non-gaseous sample to convert the sample to the predetermined gaseous form.

Abstract translation: 一种用于将非气态样品作为预定气体形式引入到分析仪器中的界面系统，该分析仪器包括接收非气态样品的基底和加热含有非气态样品的所述基底的至少一部分的定向能量源 以将样品转化为预定的气体形式。