PCR compatible nucleic acid sieving matrix
    6.
    发明授权
    PCR compatible nucleic acid sieving matrix 有权
    PCR兼容的核酸筛分基质

    公开(公告)号:US07521186B2

    公开(公告)日:2009-04-21

    申请号:US10659423

    申请日:2003-09-10

    申请人: Tammy Burd Mehta

    发明人: Tammy Burd Mehta

    IPC分类号: C12Q1/68 C12P19/34

    摘要: Sieving mediums comprising less than about 0.5% polymer, less than about 0.4% polymer, and 0.35% polymer or less are used to perform nucleic acid separations and PCR. The low polymer concentration does not inhibit PCR reactions and is sufficient for performing nucleic acids separations. Microfluidic devices are used to perform nucleic acids separations and PCR reactions in the sieving mediums described.

    摘要翻译: 使用包含少于约0.5%聚合物,小于约0.4%聚合物和0.35%聚合物或更低的筛分介质进行核酸分离和PCR。 低聚合物浓度不抑制PCR反应,并且足以进行核酸分离。 微流控装置用于在所述的筛分介质中进行核酸分离和PCR反应。

    Methods and systems for performing multiple reactions by interfacial mixing
    7.
    发明授权
    Methods and systems for performing multiple reactions by interfacial mixing 有权
    通过界面混合进行多重反应的方法和系统

    公开(公告)号:US06977163B1

    公开(公告)日:2005-12-20

    申请号:US10162140

    申请日:2002-06-04

    申请人: Tammy Burd Mehta

    发明人: Tammy Burd Mehta

    IPC分类号: C07H21/04 C12P19/34 C12Q1/68

    摘要: Methods and systems of performing multiple reactions in a high throughput format by utilizing interfacial mixing of adjacently positioned reagent slugs in a fluid conduit. Preferred applications of the methods and systems are in performing biochemical analyses, including genotyping experiments for multiple different loci on multiple different patient samples. Microfluidic systems are provided that increase throughput, automation and integration of the overall reactions to be carried out.

    摘要翻译: 通过利用相邻定位的试剂塞在流体导管中的界面混合,以高通量格式进行多重反应的方法和系统。 方法和系统的优选应用是进行生物化学分析,包括在多个不同患者样品上的多个不同基因座的基因分型实验。 提供了微流控系统,其提高了要进行的整体反应的产量,自动化和整合。