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1.发明授权Viral based transient-expression vector system that allows multiple applications 有权基于病毒的瞬态表达载体系统，允许多种应用公开(公告)号：US08389804B2
公开(公告)日：2013-03-05
申请号：US13207731
申请日：2011-08-11
Applicant: William O. Dawson , Svetlana Y. Folimonova
Inventor： William O. Dawson , Svetlana Y. Folimonova
CPC classification number: C12N15/8203 , C12N7/00 , C12N15/86 , C12N2770/00034 , C12N2770/00043
Abstract: Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.
Abstract translation: 本文公开了适于转染木本树的病毒载体，用于递送和表达有益基因。 本文具体举例说明用于转染柑橘树的载体。 载体允许表达有用的蛋白质，例如可以保护树免于疾病的蛋白质。 具体举例说明的是转染木本树的方法，其允许多次施用载体同时避免超感染排除。
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公开(公告)号：US07192740B2
公开(公告)日：2007-03-20
申请号：US10280725
申请日：2002-10-24
Applicant: Jonathan Donson , William O. Dawson , George L. Grantham , Thomas H. Turpen , Ann Myers Turpen , Stephen J. Garger, Jr. , Laurence K. Grill
Inventor： Jonathan Donson , William O. Dawson , George L. Grantham , Thomas H. Turpen , Ann Myers Turpen , Stephen J. Garger, Jr. , Laurence K. Grill
CPC classification number: C07K14/005 , C07K14/415 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/1137 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2810/609 , C12N2830/00 , C12N2830/60 , C12P41/003 , C12Y114/18001 , C12Y302/01031 , C12Y304/21069
Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates to viruses containing the viral vectors which are infective, production cells which are capable of producing the viruses or parts thereof, a host infected by the viruses of the invention, the gene products produced by expression of the viral nucleic acids and a process for the production of a desired product by growing the infected hosts.
Abstract translation: 本发明涉及选自具有编码第一病毒亚基因组启动子的天然亚基因启动子的（+）有义单链RNA病毒的重组病毒核酸，编码转录调节的病毒外壳蛋白的核酸序列 通过第一病毒亚基因组启动子，第二病毒亚基因组启动子和其转录受第二病毒亚基因组启动子调控的第二核酸序列。 重组病毒核酸的第一和第二病毒亚基因组启动子相对于彼此不具有同源序列。 重组病毒核酸提供其系统地转录宿主中的第二核酸的特别优点。 本发明包括的宿主生物包括原核生物和真核生物，特别是动物和植物。 本发明还涉及含有感染性病毒载体的病毒，能够产生病毒或其部分的生产细胞，被本发明的病毒感染的宿主，通过表达病毒核酸产生的基因产物和 通过生长受感染的宿主来生产所需产品的方法。
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公开(公告)号：US06284492B1
公开(公告)日：2001-09-04
申请号：US08483502
申请日：1995-06-07
Applicant: Jon Donson , William O. Dawson , George L. Grantham , Thomas H. Turpen , Ann M. Turpen , Stephen J. Garger , Laurence K. Grill
Inventor： Jon Donson , William O. Dawson , George L. Grantham , Thomas H. Turpen , Ann M. Turpen , Stephen J. Garger , Laurence K. Grill
IPC: C12N1511
CPC classification number: C07K14/005 , C07K14/415 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/1137 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32743 , C12N2770/32762 , C12N2770/36143 , C12N2770/36162 , C12N2810/609 , C12P41/003 , C12Y114/18001 , C12Y204/01019 , C12Y302/01031 , C12Y304/21069
Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates to viruses containing the viral vectors which are infective, production cells which are capable of producing the viruses or parts thereof, a host infected by the viruses of the invention, the gene products produced by expression of the viral nucleic acids and a process for the production of a desired product by growing the infected hosts.
Abstract translation: 本发明涉及选自具有编码第一病毒亚基因组启动子的天然亚基因启动子的（+）有义单链RNA病毒的重组病毒核酸，编码转录调节的病毒外壳蛋白的核酸序列 通过第一病毒亚基因组启动子，第二病毒亚基因组启动子和其转录受第二病毒亚基因组启动子调控的第二核酸序列。 重组病毒核酸的第一和第二病毒亚基因组启动子相对于彼此没有同源序列。 重组病毒核酸提供其系统地转录宿主中的第二核酸的特别优点。 本发明包括的宿主生物包括原核生物和真核生物，特别是动物和植物。本发明还涉及含有感染性病毒载体的病毒，能够产生病毒或其部分的生产细胞，被 本发明的病毒，通过表达病毒核酸产生的基因产物和通过生长受感染宿主生产所需产物的方法。
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公开(公告)号：US6054566A
公开(公告)日：2000-04-25
申请号：US484341
申请日：1995-06-07
Applicant: Jon Donson , William O. Dawson , George L. Granthan , Thomas H. Turpen , Ann Myers Turpen , Stephen J. Garger , Laurence K. Grill
Inventor： Jon Donson , William O. Dawson , George L. Granthan , Thomas H. Turpen , Ann Myers Turpen , Stephen J. Garger , Laurence K. Grill
IPC: C07K14/08 , C07K14/095 , C07K14/415 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/24 , C12N9/72 , C12N9/78 , C12N9/84 , C12N15/113 , C12N15/12 , C12N15/33 , C12N15/40 , C12N15/82 , C12N15/83 , C12N15/86 , C12P41/00 , C07H21/02 , C12N15/63
CPC classification number: C07K14/005 , C07K14/415 , C12N15/1137 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12P41/003 , C12Y114/18001 , C12Y302/01031 , C12Y304/21069 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2810/609 , C12N2830/00 , C12N2830/60
Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular adivantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants.The present invention also relates to viruses containing the viral vectors which are infective, production cells which are capable of producing the viruses or parts thereof, a host infected by the viruses of the invention, the gene products produced by expression of the viral nucleic acids and a process for the production of a desired product by growing the infected hosts.
Abstract translation: 本发明涉及选自具有编码第一病毒亚基因组启动子的天然亚基因启动子的（+）有义单链RNA病毒的重组病毒核酸，编码转录调节的病毒外壳蛋白的核酸序列 通过第一病毒亚基因组启动子，第二病毒亚基因组启动子和其转录受第二病毒亚基因组启动子调控的第二核酸序列。 重组病毒核酸的第一和第二病毒亚基因组启动子相对于彼此不具有同源序列。 重组病毒核酸提供其系统地转录宿主中的第二核酸的特别优点。 本发明包括的宿主生物包括原核生物和真核生物，特别是动物和植物。 本发明还涉及含有感染性病毒载体的病毒，能够产生病毒或其部分的生产细胞，被本发明的病毒感染的宿主，通过表达病毒核酸产生的基因产物和 通过生长受感染的宿主来生产所需产品的方法。
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公开(公告)号：US5866785A
公开(公告)日：1999-02-02
申请号：US482920
申请日：1995-06-07
Applicant: Jon Donson , William O. Dawson , George L. Granthan , Thomas H. Turpen , Ann Myers Turpen , Stephen J. Garger , Laurence K. Grill
Inventor： Jon Donson , William O. Dawson , George L. Granthan , Thomas H. Turpen , Ann Myers Turpen , Stephen J. Garger , Laurence K. Grill
IPC: C07K14/08 , C07K14/095 , C07K14/415 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/24 , C12N9/72 , C12N9/78 , C12N9/84 , C12N15/113 , C12N15/12 , C12N15/33 , C12N15/40 , C12N15/82 , C12N15/83 , C12N15/86 , C12P41/00 , A01H5/00
CPC classification number: C12Y114/18001 , C07K14/005 , C07K14/415 , C12N15/1137 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12P41/003 , C12Y302/01031 , C12Y304/21069 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2810/609
Abstract: The present invention is directed to recombinant plant viral nucleic acids and to hosts infected thereby. The recombinant plant viral nucleic acids comprise a native plant viral subgenomic promoter, at least one non-native plant viral subgenomic promoter, a plant viral coat protein coding sequence, and optionally, at least one non-native nucleic acid sequence to be transcribed or expressed in the infected host plant. The recombinant plant viral nucleic acids are stable, capable of systemic infection and capable of stable transcription or expression in the plant host of the non-native nucleic acid sequences.
Abstract translation: 本发明涉及重组植物病毒核酸和由此感染的宿主。 重组植物病毒核酸包含天然植物病毒亚基因组启动子，至少一种非天然植物病毒亚基因组启动子，植物病毒外壳蛋白编码序列和任选的至少一个待转录或表达的非天然核酸序列 在被感染的宿主植物中。 重组植物病毒核酸是稳定的，能够全身感染并且能够在非天然核酸序列的植物宿主中稳定转录或表达。
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Patent Agency Ranking6.发明授权Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes 失效具有异源亚基因启动子的植物病毒载体用于外源基因的全身表达
公开(公告)号：US5316931A
公开(公告)日：1994-05-31
申请号：US923692
申请日：1992-07-31
Applicant: Jon Donson , William O. Dawson , George L. Granthan , Thomas H. Turpen , Ann M. Turpen , Stephen J. Garger , Laurence K. Grill
Inventor： Jon Donson , William O. Dawson , George L. Granthan , Thomas H. Turpen , Ann M. Turpen , Stephen J. Garger , Laurence K. Grill
IPC: C07K14/08 , C07K14/095 , C07K14/415 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/24 , C12N9/72 , C12N9/78 , C12N9/84 , C12N15/113 , C12N15/12 , C12N15/33 , C12N15/40 , C12N15/82 , C12N15/83 , C12N15/86 , C12P41/00 , A01H5/00 , C07H21/04
CPC classification number: C12Y114/18001 , C07K14/005 , C07K14/415 , C12N15/1137 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12P41/003 , C12Y302/01031 , C12Y304/21069 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2810/609
Abstract: The present invention is directed to recombinant plant viral nucleic acids and to hosts infected thereby. The recombinant plant viral nucleic acids comprise a native plant viral subgenomic promoter, at least one non-native plant viral subgenomic promoter, a plant viral coat protein coding sequence, and optionally, at least one non-native nucleic acid sequence to be transcribed or expressed in the infected host plant. The recombinant plant viral nucleic acids are stable, capable of systemic infection and capable of stable transcription or expression in the plant host of the non-native nucleic acid sequences.
Abstract translation: 本发明涉及重组植物病毒核酸和由此感染的宿主。 重组植物病毒核酸包含天然植物病毒亚基因组启动子，至少一种非天然植物病毒亚基因组启动子，植物病毒外壳蛋白编码序列和任选的至少一个待转录或表达的非天然核酸序列 在被感染的宿主植物中。 重组植物病毒核酸是稳定的，能够全身感染并且能够在非天然核酸序列的植物宿主中稳定转录或表达。
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公开(公告)号：US08629334B2
公开(公告)日：2014-01-14
申请号：US12174159
申请日：2008-07-16
Applicant: William O. Dawson , Svetlana Y. Folimonova , Alexey S. Folimonov
Inventor： William O. Dawson , Svetlana Y. Folimonova , Alexey S. Folimonov
CPC classification number: C12N15/8203 , C12N2770/00043
Abstract: Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes with increased stability. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease.
Abstract translation: 本文公开了适于转染木本树的病毒载体，用于递送和表达具有增加的稳定性的有益基因。 本文具体举例说明用于转染柑橘树的载体。 载体允许表达有用的蛋白质，例如可以保护树免于疾病的蛋白质。
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公开(公告)号：US06448046B1
公开(公告)日：2002-09-10
申请号：US09726651
申请日：2000-11-28
Applicant: Jon Donson , William O. Dawson , George L. Grantham , Thomas H. Turpen , Ann M. Turpen , Stephen J. Garger , Laurence K. Grill
Inventor： Jon Donson , William O. Dawson , George L. Grantham , Thomas H. Turpen , Ann M. Turpen , Stephen J. Garger , Laurence K. Grill
IPC: C12N1511
CPC classification number: C12Y114/18001 , C07K14/005 , C07K14/415 , C12N9/0059 , C12N9/0071 , C12N9/1033 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/8203 , C12N15/8289 , C12N15/86 , C12N2770/36122 , C12N2830/00 , C12N2830/60 , C12P41/003 , C12Y203/01028 , C12Y302/01031 , C12Y304/21069
Abstract: The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates to viruses containing the viral vectors which are infective, production cells which are capable of producing the viruses or parts thereof, a host infected by the viruses of the invention, the gene products produced by expression of the viral nucleic acids and a process for the production of a desired product by growing the infected hosts.
Abstract translation: 本发明涉及选自具有编码第一病毒亚基因组启动子的天然亚基因启动子的（+）有义单链RNA病毒的重组病毒核酸，编码转录调节的病毒外壳蛋白的核酸序列 通过第一病毒亚基因组启动子，第二病毒亚基因组启动子和其转录受第二病毒亚基因组启动子调控的第二核酸序列。 重组病毒核酸的第一和第二病毒亚基因组启动子相对于彼此不具有同源序列。 重组病毒核酸提供其系统地转录宿主中的第二核酸的特别优点。 本发明包括的宿主生物包括原核生物和真核生物，特别是动物和植物。 本发明还涉及含有感染性病毒载体的病毒，能够产生病毒或其部分的生产细胞，被本发明的病毒感染的宿主，通过表达病毒核酸产生的基因产物和 通过生长受感染的宿主来生产所需产品的方法。
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公开(公告)号：US5889190A
公开(公告)日：1999-03-30
申请号：US480432
申请日：1995-06-07
Applicant: Jon Donson , William O. Dawson , George L. Grantham , Thomas H. Turpen , Ann Myers Turpen , Stephen J. Garger , Laurence K. Grill
Inventor： Jon Donson , William O. Dawson , George L. Grantham , Thomas H. Turpen , Ann Myers Turpen , Stephen J. Garger , Laurence K. Grill
IPC: C07K14/08 , C07K14/095 , C07K14/415 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/24 , C12N9/72 , C12N9/78 , C12N9/84 , C12N15/113 , C12N15/12 , C12N15/33 , C12N15/40 , C12N15/82 , C12N15/83 , C12N15/86 , C12P41/00 , A01H5/00
CPC classification number: C12Y114/18001 , C07K14/005 , C07K14/415 , C12N15/1137 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12P41/003 , C12Y302/01031 , C12Y304/21069 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36122 , C12N2770/36143 , C12N2770/36162 , C12N2810/609
Abstract: The present invention is directed to recombinant plant viral nucleic acids and to hosts infected thereby. The recombinant plant viral nucleic acids comprise a native plant viral subgenomic promoter, at least one non-native plant viral subgenomic promoter, a plant viral coat protein coding sequence, and optionally, at least one non-native nucleic acid sequence to be transcribed or expressed in the infected host plant. The recombinant plant viral nucleic acids are stable, capable of systemic infection and capable of stable transcription or expression in the plant host of the non-native nucleic acid sequences.
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10.发明申请VIRAL BASED TRANSIENT-EXPRESSION VECTOR SYSTEM THAT ALLOWS MULTIPLE APPLICATIONS 有权基于VIRAL的瞬态表示向量系统，允许多个应用公开(公告)号：US20120204287A1
公开(公告)日：2012-08-09
申请号：US13207731
申请日：2011-08-11
Applicant: William O. Dawson , Svetlana Y. Folimonova
Inventor： William O. Dawson , Svetlana Y. Folimonova
IPC: C12N15/83
CPC classification number: C12N15/8203 , C12N7/00 , C12N15/86 , C12N2770/00034 , C12N2770/00043
Abstract: Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.
Abstract translation: 本文公开了适于转染木本树的病毒载体，用于递送和表达有益基因。 本文具体举例说明用于转染柑橘树的载体。 载体允许表达有用的蛋白质，例如可以保护树免于疾病的蛋白质。 具体举例说明的是转染木本树的方法，其允许多次施用载体同时避免超感染排除。
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