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公开(公告)号：US09890205B2
公开(公告)日：2018-02-13
申请号：US14555060
申请日：2014-11-26
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Peter Gagnon
CPC classification number: C07K16/00 , B01D15/363 , C07K1/16 , C07K1/165 , C07K1/18 , C07K16/22 , C07K16/241 , C07K16/32
Abstract: A method of purifying a sample containing a desired protein includes the steps of (i) providing a packed chromatographic column having positively charged porous particles, (ii) equilibrating the column to the conditions to which the desired protein in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied to the packed chromatographic column is less than or equal to the interparticle space of the positively charged porous particles within the packed chromatographic column, (iv) eluting the desired protein from the packed chromatographic column, where the desired protein is in a purer state and in the conditions to which the packed chromatographic column was equilibrated; where the desired protein is an antibody, an antibody fragment, an antibody derivative, or an antibody fusion protein.
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2.发明申请CHROMATOGRAPHIC PURIFICATION OF POLYNUCLEOTIDES WITH NEGATIVELY CHARGED PARTICLES 有权带有负电荷颗粒的多核苷酸的色谱纯化公开(公告)号：US20150148528A1
公开(公告)日：2015-05-28
申请号：US14555257
申请日：2014-11-26
Applicant: Agency for Science, Technology and Research
Inventor： Peter Gagnon
IPC: C07H21/04
CPC classification number: C12N15/101 , B01D15/362 , C07H21/04 , C12N15/1006 , C12N15/1017
Abstract: A method of purifying a sample that includes a polynucleotide includes the steps of (i) providing a packed chromatographic column having negatively charged porous particles, (ii) equilibrating the column to the conditions to which the polynucleotide in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied to the packed chromatographic column is less than or equal to the interparticle space of the negatively charged porous particles within the packed chromatographic column, (iv) eluting the polynucleotide from the packed chromatographic column, where the polynucleotide is in a purer state and in the conditions to which the packed chromatographic column was equilibrated.
Abstract translation: 纯化包含多核苷酸的样品的方法包括以下步骤：（i）提供具有带负电荷的多孔颗粒的填充色谱柱，（ii）将色谱柱平衡到待洗脱的样品中的多核苷酸的条件，（iii ）使样品与填充色谱柱接触，使得施加到填充色谱柱的样品体积小于或等于填充色谱柱内带负电荷的多孔颗粒的颗粒间间隔，（iv）从包装的色谱柱中洗脱多核苷酸 色谱柱，其中多核苷酸处于更纯的状态和在填充色谱柱平衡的条件下。
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3.发明申请MIXED MULTIFUNCTIONAL METAL AFFINITY SURFACES FOR REDUCING AGGREGATE CONTENT IN PROTEIN PREPARATIONS 审中-公开用于降低蛋白质制备中的聚集含量的混合多功能金属亲合表面公开(公告)号：US20150147243A1
公开(公告)日：2015-05-28
申请号：US14555165
申请日：2014-11-26
Applicant: Agency for Science, Technology and Research
Inventor： Peter Gagnon
CPC classification number: C07K16/32 , B01D15/34 , B01D15/36 , B01D15/362 , B01D15/363 , B01D15/3809 , B01D15/3828 , B01J20/3202 , B01J20/3265 , B01J20/3285 , B01J39/26 , B01J41/20 , B01J47/014 , B01J47/04 , C07K1/165 , C07K1/22 , C07K16/00 , Y10T428/2913
Abstract: Compositions for reducing the aggregate content of a protein preparation include a first substrate having a first surface-bound ligand possessing a metal affinity functionality and a second surface-bound ligand optionally provided on a second substrate and having an aggregate charge opposite to that of the metal affinity functionality of the first substrate, wherein the first surface-bound ligand and the second surface-bound ligand are positioned such that the protein preparation may contact both the first surface-bound ligand and the second surface-bound ligand simultaneously.
Abstract translation: 用于减少蛋白质制剂的骨料含量的组合物包括具有第一表面结合配体的具有金属亲和功能的第一底物和任选地设置在第二底物上并且具有与金属相反的聚集体电荷的第二表面结合配体的第一底物 所述第一底物的亲和功能性，其中所述第一表面结合配体和所述第二表面结合配体被定位使得所述蛋白质制剂可以同时接触所述第一表面结合配体和所述第二表面结合配体。
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公开(公告)号：US09637724B2
公开(公告)日：2017-05-02
申请号：US14555288
申请日：2014-11-26
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Peter Gagnon
IPC: A61K39/00 , A01N37/18 , A01N43/16 , A01N43/78 , A01N43/90 , C12N7/00 , C07H1/08 , C07K1/36 , C07K14/77 , C07K16/00 , C12N5/071 , B01D15/26 , B01D15/38 , B01J20/286 , B01J20/32 , C07K1/22
CPC classification number: C12N7/00 , B01D15/265 , B01D15/3804 , B01J20/286 , B01J20/3251 , C07H1/08 , C07K1/22 , C07K1/36 , C07K14/77 , C07K16/00 , C12N5/0602 , C12N2770/24151 , C12N2795/00051
Abstract: A method of selectively separating a biological target from a sample including the biological target material or suspected of including the biological target includes the steps of (i) providing a solid including ureide moieties at its surface, (ii) contacting the sample with the solid, whereby a substantial fraction of the biological target in the sample binds to the ureide moieties, and (iii) separating the solid from the sample.
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公开(公告)号：US20150184132A1
公开(公告)日：2015-07-02
申请号：US14555288
申请日：2014-11-26
Applicant: Agency for Science, Technology and Research
Inventor： Peter Gagnon
CPC classification number: C12N7/00 , B01D15/265 , B01D15/3804 , B01J20/286 , B01J20/3251 , C07H1/08 , C07K1/22 , C07K1/36 , C07K14/77 , C07K16/00 , C12N5/0602 , C12N2770/24151 , C12N2795/00051
Abstract: A method of selectively separating a biological target from a sample including the biological target material or suspected of including the biological target includes the steps of (i) providing a solid including ureide moieties at its surface, (ii) contacting the sample with the solid, whereby a substantial fraction of the biological target in the sample binds to the ureide moieties, and (iii) separating the solid from the sample.
Abstract translation: 选择性分离生物靶与包含生物靶材的样品或怀疑包括生物靶的方法包括以下步骤：（i）在其表面提供包含脲基部分的固体，（ii）使样品与固体接触， 其中样品中大部分生物靶标结合于脲基部分，和（iii）从样品中分离固体。
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Patent Agency Ranking
6.发明申请METHODS FOR REDUCING LEVELS OF PROTEIN-CONTAMINANT COMPLEXES AND AGGREGATES IN PROTEIN PREPARATIONS BY TREATMENT WITH ELECTROPOSITIVE ORGANIC ADDITIVES 审中-公开通过用电解有机添加剂处理来降低蛋白质污染物复合物和蛋白质制剂水平的方法公开(公告)号：US20150148526A1
公开(公告)日：2015-05-28
申请号：US14555224
申请日：2014-11-26
Applicant: Agency for Science, Technology and Research
Inventor： Peter Gagnon
IPC: C07K16/00
Abstract: Methods for reduction of aggregate levels in antibody and other protein preparations through treatment with low concentrations of electropositive organic additives (e.g., ethacridine, chlorhexidine, or polyethylenimine) in combination with ureides (e.g., urea, uric acid, or allantoin) or organic modulators (e.g., nonionic organic polymers, surfactants, organic solvent or ureides). Some aspects of the invention relate to methods for reducing the level of aggregates in conjunction with clarification of cell culture harvest. It further relates to the integration of these capabilities with other purification methods to achieve the desired level of final purification.
Abstract translation: 通过用低浓度的正电性有机添加剂（例如，ethacridine，氯己定或聚乙烯亚胺）与脲类（例如尿素，尿酸或尿囊素）或有机调节剂（如尿素，尿素或尿囊素）联合治疗，降低抗体和其他蛋白质制剂中骨料含量的方法 例如非离子有机聚合物，表面活性剂，有机溶剂或脲类）。 本发明的一些方面涉及与细胞培养收获的澄清一起降低聚集体水平的方法。 它还涉及这些能力与其他纯化方法的整合以实现期望的最终纯化水平。
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公开(公告)号：US10246506B2
公开(公告)日：2019-04-02
申请号：US14555224
申请日：2014-11-26
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Peter Gagnon
Abstract: Methods for reduction of aggregate levels in antibody and other protein preparations through treatment with low concentrations of electropositive organic additives (e.g., ethacridine, chlorhexidine, or polyethylenimine) in combination with ureides (e.g., urea, uric acid, or allantoin) or organic modulators (e.g., nonionic organic polymers, surfactants, organic solvent or ureides). Some aspects of the invention relate to methods for reducing the level of aggregates in conjunction with clarification of cell culture harvest. It further relates to the integration of these capabilities with other purification methods to achieve the desired level of final purification.
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公开(公告)号：US09920125B2
公开(公告)日：2018-03-20
申请号：US14555099
申请日：2014-11-26
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Peter Gagnon
CPC classification number: C07K16/2863 , C07K1/165 , C07K14/755 , C07K16/00 , C07K16/065 , C07K16/18 , C07K16/32 , C07K2317/55
Abstract: Methods for reduction of aggregate levels in antibody and other protein preparations comprising the steps of treatment with an organic multivalent cation (e.g., ethacridine, chlorhexidine, or polyethylenimine) and treatment with a composition having a combination of surfaces bearing electronegative chemical moieties and surfaces bearing electropositive chemical moieties.
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公开(公告)号：US09890376B2
公开(公告)日：2018-02-13
申请号：US14555257
申请日：2014-11-26
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Peter Gagnon
CPC classification number: C12N15/101 , B01D15/362 , C07H21/04 , C12N15/1006 , C12N15/1017
Abstract: A method of purifying a sample that includes a polynucleotide includes the steps of (i) providing a packed chromatographic column having negatively charged porous particles, (ii) equilibrating the column to the conditions to which the polynucleotide in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied to the packed chromatographic column is less than or equal to the interparticle space of the negatively charged porous particles within the packed chromatographic column, (iv) eluting the polynucleotide from the packed chromatographic column, where the polynucleotide is in a purer state and in the conditions to which the packed chromatographic column was equilibrated.
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10.发明申请METHODS FOR USE OF MIXED MULTIFUNCTIONAL SURFACES FOR REDUCING AGGREGATE CONTENT IN PROTEIN PREPARATIONS 有权使用混合多功能表面降低蛋白质制备中聚集体含量的方法公开(公告)号：US20150183879A1
公开(公告)日：2015-07-02
申请号：US14555099
申请日：2014-11-26
Applicant: Agency for Science, Technology and Research
Inventor： Peter Gagnon
IPC: C07K16/28 , C07K14/755 , C07K16/18
CPC classification number: C07K16/2863 , C07K1/165 , C07K14/755 , C07K16/00 , C07K16/065 , C07K16/18 , C07K16/32 , C07K2317/55
Abstract: Methods for reduction of aggregate levels in antibody and other protein preparations comprising the steps of treatment with an organic multivalent cation (e.g., ethacridine, chlorhexidine, or polyethylenimine) and treatment with a composition having a combination of surfaces bearing electronegative chemical moieties and surfaces bearing electropositive chemical moieties.
Abstract translation: 用于降低抗体和其它蛋白质制剂中聚集体水平的方法包括用有机多价阳离子（例如，ethacridine，氯己定或聚乙烯亚胺）处理的步骤和用具有带电电荷化学部分的表面和具有正电性的表面组合的组合物 化学部分。
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