公开(公告)号：US20180016624A1

公开(公告)日：2018-01-18

申请号：US15658016

申请日：2017-07-24

Applicant: Applied Biosystems, LLC

Inventor： Min YUE ,  David M. LIU ,  Joy ROY ,  Yuh-Min CHIANG ,  Joon Mo YANG ,  Dennis LEHTO ,  Charles S. VANN ,  Nigel P. BEARD ,  Ian A. HARDING ,  John R. Van Camp ,  Alexander DROMARETSKY ,  Sergey V. ERMAKOV ,  Mark F. OLDHAM ,  Maryam SHARIATI ,  Umberto ULMANELLA

IPC: C12Q1/68 ,  G01N35/02 ,  B01L3/00

CPC classification number: C12Q1/6806 ,  B01J2219/00317 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502723 ,  B01L3/50853 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/041 ,  B01L2300/048 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0694 ,  G01N35/028 ,  Y10T436/2575

Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

公开(公告)号：US20200149099A1

公开(公告)日：2020-05-14

申请号：US16655911

申请日：2019-10-17

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Willy K. WIYATNO ,  Linda LEE ,  David COX ,  James NURSE ,  Douglas GREINER ,  Dennis LEHTO ,  Janusz WOJTOWICZ ,  Sam WOO ,  Richard REEL

IPC: C12Q1/6869 ,  B01L3/00 ,  F15C5/00 ,  F16K99/00 ,  G01N35/08 ,  G01N1/14 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N27/447

Abstract: A system may include a first conduit configured to form a first batch of discrete volumes of aqueous fluid separated by spacing liquid disposed between consecutive volumes of aqueous fluid, the spacing liquid being immiscible with the aqueous fluid volumes; a second conduit, fluidically coupled to the first conduit, the second conduit configured to statically hold the first batch of discrete volumes of aqueous fluid; and a third conduit configured to receive the first batch of discrete volumes of aqueous fluid from the second conduit. The third conduit can be configured to transfer the discrete volumes of aqueous fluid of the first batch for downstream processing.

公开(公告)号：US20130183210A1

公开(公告)日：2013-07-18

申请号：US13724079

申请日：2012-12-21

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Willy WIYATNO ,  Linda LEE ,  David COX ,  Mark OLDHAM ,  Jim NURSE ,  Douglas GREINER ,  Dennis LEHTO ,  Janusz WOJTOWICZ ,  Sam WOO ,  Richard REEL

IPC: G01N1/14

CPC classification number: C12Q1/6869 ,  B01J2219/00353 ,  B01J2219/0036 ,  B01J2219/00364 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/0293 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0421 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6874 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0011 ,  F16K99/0013 ,  F16K2099/0084 ,  G01N1/14 ,  G01N27/44743 ,  G01N27/44769 ,  G01N35/08 ,  Y10T137/0318 ,  Y10T137/4259 ,  Y10T137/85978 ,  Y10T137/85986 ,  Y10T137/86863 ,  Y10T436/2575 ,  C12Q2535/101

Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

Abstract translation: 在本申请中描述的各种实施例涉及用于在管道内产生与第二流体不混溶的一种或多种流体的离散体积的装置，系统和方法。 离散体积可用于涉及小体积的生物化学或分子生物学过程，例如微升体积，纳升级体积或更小体积。 该系统可以包括一种装置，该装置包括至少一个导管，其操作地连接到一个或多个泵，用于提供通过与离散体积的流体不混溶的流体彼此分离的离散体积，例如水不混溶流体 - 油分离体积。