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公开(公告)号：US20160109338A1
公开(公告)日：2016-04-21
申请号：US14978935
申请日：2015-12-22
Applicant: Advanced Liquid Logic, Inc.
Inventor： Ramakrishna Sista , Vamsee K. Pamula , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: G01N1/38
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
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公开(公告)号：US09086345B2
公开(公告)日：2015-07-21
申请号：US14308110
申请日：2014-06-18
Applicant: Advanced Liquid Logic, Inc.
Inventor： Ramakrishna Sista , Vamsee K. Pamula , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: G01N15/06 , G01N33/00 , G01N33/48 , G01N1/38 , B01F11/00 , B01F13/00 , B01L3/00 , B01L7/00 , G01N35/10
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.
Abstract translation: 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在大气场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。
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公开(公告)号：US20140302580A1
公开(公告)日：2014-10-09
申请号：US14308110
申请日：2014-06-18
Applicant: Advanced Liquid Logic, Inc.
Inventor： Ramakrishna Sista , Vamsee K. Pamula , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: G01N1/38
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.
Abstract translation: 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在大气场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。
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4.发明申请公开(公告)号：US20170003282A1
公开(公告)日：2017-01-05
申请号：US15266693
申请日：2016-09-15
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: G01N33/543 , B01F13/00 , B01F13/08 , B01L3/00
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
Abstract translation: 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中，本发明利用具有顶部和底部基底的液滴致动器，分别存在于顶部和底部基底附近的多个磁场，其中至少一个磁场可选择性地改变，并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间，并且至少一个磁场被选择性地改变。
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公开(公告)号：US09494498B2
公开(公告)日：2016-11-15
申请号：US14978935
申请日：2015-12-22
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: B01L3/00 , B01L3/02 , G01N1/10 , G01N15/06 , G01N33/00 , G01N33/48 , G01N1/38 , B01F11/00 , B01F13/00 , G01N33/543 , B01L7/00 , G01N35/10
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
Abstract translation: 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中，本发明利用具有顶部和底部基底的液滴致动器，分别存在于顶部和底部基底附近的多个磁场，其中至少一个磁场可选择性地改变，并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间，并且至少一个磁场被选择性地改变。
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Patent Agency Ranking
公开(公告)号：US20150285792A1
公开(公告)日：2015-10-08
申请号：US14746276
申请日：2015-06-22
Applicant: Advanced Liquid Logic, Inc.
Inventor： Ramakrishna Sista , Vamsee K. Pamula , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: G01N33/543
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
Abstract translation: 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中，本发明利用具有顶部和底部基底的液滴致动器，分别存在于顶部和底部基底附近的多个磁场，其中至少一个磁场可选择性地改变，并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间，并且至少一个磁场被选择性地改变。
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公开(公告)号：US10139403B2
公开(公告)日：2018-11-27
申请号：US15266693
申请日：2016-09-15
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: B01L3/00 , G01N1/00 , G01N1/10 , G01N15/06 , G01N1/18 , G01N33/543 , B01F11/00 , B01F13/00 , G01N1/38 , B01F13/08 , B01L7/00 , G01N35/10
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
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公开(公告)号：US09377455B2
公开(公告)日：2016-06-28
申请号：US14746276
申请日：2015-06-22
Applicant: Advanced Liquid Logic, Inc.
Inventor： Ramakrishna Sista , Vamsee K. Pamula , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: G01N15/06 , G01N33/00 , G01N33/48 , G01N33/543 , B01F11/00 , B01F13/00 , B01L3/00 , G01N1/38 , B01L7/00 , G01N35/10
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
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公开(公告)号：US09952177B2
公开(公告)日：2018-04-24
申请号：US14795304
申请日：2015-07-09
Applicant: Advanced Liquid Logic, Inc. , Duke University
Inventor： Michael G. Pollack , Vijay Srinivasan , Zhishan Hua , Hon Lung Chu , Michael Hauser , Allen Eckhardt
IPC: G01N27/447 , G01N27/453 , B01L3/00 , B01L7/00
CPC classification number: G01N27/44743 , B01L3/502715 , B01L3/502792 , B01L7/525 , B01L2200/025 , B01L2200/027 , B01L2300/088 , B01L2300/0887 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/043 , B01L2400/0457 , B01L2400/046 , G01N27/44791
Abstract: The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.
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10.发明申请公开(公告)号：US20150323496A1
公开(公告)日：2015-11-12
申请号：US14795304
申请日：2015-07-09
Applicant: Advanced Liquid Logic, Inc. , Duke University
Inventor： Michael G. Pollack , Vijay Srinivasan , Zhishan Hua , Hon Lung Chu , Michael Hauser , Allen Eckhardt
IPC: G01N27/447 , B01L3/00
CPC classification number: G01N27/44743 , B01L3/502715 , B01L3/502792 , B01L7/525 , B01L2200/025 , B01L2200/027 , B01L2300/088 , B01L2300/0887 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/043 , B01L2400/0457 , B01L2400/046 , G01N27/44791
Abstract: The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.
Abstract translation: 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术，液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶，捕获并随后处理电泳凝胶的产物，或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中，在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定，例如通过实时PCR的核酸（例如DNA）定量和一个或多个样品处理操作，例如样品稀释。 在一个实施方案中，电泳凝胶可以集成在液滴致动器的顶部基底上。
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