公开(公告)号：US10913068B2

公开(公告)日：2021-02-09

申请号：US15557957

申请日：2016-03-14

Applicant: Nanyang Technological University ,  Agency for Science, Technology And Research ,  The Methodist Hospital

Inventor： Joseph Sylvester Chang ,  Geok Soon Lim ,  Lei Zhang ,  Zhiping Wang ,  Ruige Wu ,  Stephen T. C. Wong ,  Kemi Cui

IPC: B01L7/00 ,  C12Q1/686 ,  A61B5/15 ,  A61B5/153 ,  C12Q1/6851

Abstract: A testing device is provided. The testing device includes a capturing tool and a microfluidic chip having a plurality of chambers connected in a network, a sample receiving port connected to the network, and a guide structure configured to receive the capturing tool, wherein the capturing tool is configured to capture sample in a distal position from the guide structure and further configured to transfer the captured sample to the sample receiving port in a proximal position from the guide structure.

公开(公告)号：US09933390B2

公开(公告)日：2018-04-03

申请号：US14647346

申请日：2013-12-13

Applicant: Agency for Science, Technology and Research

Inventor： Ruige Wu ,  Pin Chuan Chen ,  Zhiping Wang

IPC: G01N27/447 ,  C12Q1/68

CPC classification number: G01N27/44739 ,  C12Q1/6806 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44778 ,  G01N27/44791 ,  C12Q2565/629

Abstract: A device for extracting at least one analyte may include: a sample reservoir configured to contain a sample comprising at least one target analyte and interfering materials; at least one extraction chamber connected to the sample reservoir; at least one porous structure lining one or more sides of the at least one extraction chamber; and a voltage source configured to provide a first voltage and a second voltage, wherein, when the first voltage is provided, the at least one target analyte and the interfering materials move towards the at least one extraction chamber or to a predetermined area from the at least one extraction chamber, wherein, when the second voltage is provided, the interfering materials pass through and exit the at least one extraction chamber, and the at least one target analyte is stopped from exiting the at least one extraction chamber by means of the at least one porous structure.

公开(公告)号：US11566212B2

公开(公告)日：2023-01-31

申请号：US16324571

申请日：2017-08-10

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Paul Bigliardi ,  Zhiping Wang ,  Ruige Wu ,  Massimo Alberti ,  Bo Wu ,  Mei Bigliardi-Qi ,  Sriram Gopu ,  Srinivas Ramasamy ,  Yuri Hebert Dancik

IPC: B01L7/00 ,  C12M3/06 ,  C12M3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/42 ,  B01L3/00 ,  B01L9/00 ,  G01N33/50 ,  C12N5/00 ,  C12N5/071

Abstract: The present disclosure describes a microfluidic chip for culturing and in vitro testing of 3D organotypic cultures. The tests may be performed directly on the organotypic culture in the microfluidic chip. The microfluidic chip includes at least one microfluidic unit which includes two fluidic compartments, such as upper and lower, separated by a permeable supporting structure, one or more access opening for the fluidic compartments, and a set of lids interchangeable with a set of insets. The permeable support structure serves as a support for the organotypic culture. The upper and lower compartments may include inlets and outlets which allow fluids to be perfused into the lower compartment and fluids to be perfused into the upper compartment. The access opening may be closed with a lid or accommodate an inset.

公开(公告)号：US10024820B2

公开(公告)日：2018-07-17

申请号：US14658088

申请日：2015-03-13

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Ruige Wu ,  Zhiping Wang ,  Sai Kiang Lim ,  Yen Peng Daphne Seah

IPC: G01N27/447 ,  B65B3/04

Abstract: According to embodiments of the present invention, a microfluidic device for gel electrophoresis is provided. The microfluidic device includes a sample channel configured to receive a sample; a stacking channel comprising a preloaded stacking reagent; and a separation channel comprising a preloaded separation reagent, wherein the preloaded stacking reagent has a physical characteristic different from that of the preloaded separation reagent; and wherein the sample channel, the stacking channel and the separation channel are in fluid communication with one another. According to further embodiments of the present invention, a method of manufacturing a microfluidic device for gel electrophoresis is also provided.

公开(公告)号：US20150323498A1

公开(公告)日：2015-11-12

申请号：US14647346

申请日：2013-12-13

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Ruige Wu ,  Pin Chuan Chen ,  Zhiping Wang

IPC: G01N27/447

CPC classification number: G01N27/44739 ,  C12Q1/6806 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44778 ,  G01N27/44791 ,  C12Q2565/629

Abstract: A device for extracting at least one analyte may include: a sample reservoir configured to contain a sample comprising at least one target analyte and interfering materials; at least one extraction chamber connected to the sample reservoir; at least one porous structure lining one or more sides of the at least one extraction chamber; and a voltage source configured to provide a first voltage and a second voltage, wherein, when the first voltage is provided, the at least one target analyte and the interfering materials move towards the at least one extraction chamber or to a predetermined area from the at least one extraction chamber, wherein, when the second voltage is provided, the interfering materials pass through and exit the at least one extraction chamber, and the at least one target analyte is stopped from exiting the at least one extraction chamber by means of the at least one porous structure.

Abstract translation: 用于提取至少一种分析物的装置可以包括：样品储存器，其被配置为容纳包含至少一种目标分析物和干扰材料的样品; 至少一个提取室连接到样品储存器; 在所述至少一个提取室的一个或多个侧面上的至少一个多孔结构; 以及电压源，其被配置为提供第一电压和第二电压，其中，当提供所述第一电压时，所述至少一个目标分析物和所述干扰材料朝向所述至少一个提取室移动或者从所述至少一个提取室移动到预定区域 至少一个提取室，其中当所述第二电压被提供时，所述干扰材料通过并离开所述至少一个提取室，并且所述至少一个目标分析物被停止以通过所述至少一个提取室离开所述至少一个提取室 至少一个多孔结构。