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公开(公告)号：US10913068B2
公开(公告)日：2021-02-09
申请号：US15557957
申请日：2016-03-14
申请人： Nanyang Technological University , Agency for Science, Technology And Research , The Methodist Hospital
发明人： Joseph Sylvester Chang , Geok Soon Lim , Lei Zhang , Zhiping Wang , Ruige Wu , Stephen T. C. Wong , Kemi Cui
IPC分类号： B01L7/00 , C12Q1/686 , A61B5/15 , A61B5/153 , C12Q1/6851
摘要： A testing device is provided. The testing device includes a capturing tool and a microfluidic chip having a plurality of chambers connected in a network, a sample receiving port connected to the network, and a guide structure configured to receive the capturing tool, wherein the capturing tool is configured to capture sample in a distal position from the guide structure and further configured to transfer the captured sample to the sample receiving port in a proximal position from the guide structure.
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公开(公告)号：US09364804B2
公开(公告)日：2016-06-14
申请号：US14010024
申请日：2013-08-26
发明人： Huanming Xia , Zhiping Wang , Wei Wang
CPC分类号： B01F13/0059 , B01F11/0051
摘要： According to various embodiments, a microfluidic agitator device may be provided. The microfluidic agitator device may include: an air inlet; an air outlet; an elastic diaphragm provided between the air inlet and the air outlet and configured to oscillate if an airflow from the air inlet to the air outlet is provided; and a chamber coupled to the elastic diaphragm.
摘要翻译： 根据各种实施例，可以提供微流体搅拌装置。 微流体搅拌装置可以包括：空气入口; 一个出风口 设置在所述空气入口和所述空气出口之间并且被配置为当从所述空气入口到所述空气出口的气流被提供时振荡的弹性隔膜; 以及耦合到弹性隔膜的腔室。
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公开(公告)号：US20150223433A1
公开(公告)日：2015-08-13
申请号：US14619649
申请日：2015-02-11
CPC分类号： A01K29/005 , A01K1/031 , A01K67/033 , G06K9/00335 , G06K9/00362
摘要： A method of monitoring activity of an animal, the method comprising the steps of: retaining the animal in an enclosed chamber; controlling a food supply to the animal in the enclosed chamber as a first stimulus; and monitoring the activity of the animal in response to the first stimulus, wherein monitoring the activity of the animal comprises analysing change in a volume of food in the food supply and location of the animal in the enclosed chamber over a predetermined period of time.
摘要翻译： 一种监测动物活动的方法，所述方法包括以下步骤：将动物保持在封闭的室内; 控制在封闭室内的动物的食物供应作为第一刺激; 以及响应于所述第一刺激来监测动物的活动，其中监测动物的活动包括在预定时间段内分析所述食物供应中食物的量的变化和所述封闭室中的动物的位置。
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公开(公告)号：US20140056096A1
公开(公告)日：2014-02-27
申请号：US14010024
申请日：2013-08-26
发明人： Huanming Xia , Zhiping Wang , Wei Wang
IPC分类号： B01F13/00
CPC分类号： B01F13/0059 , B01F11/0051
摘要： According to various embodiments, a microfluidic agitator device may be provided. The microfluidic agitator device may include: an air inlet; an air outlet; an elastic diaphragm provided between the air inlet and the air outlet and configured to oscillate if an airflow from the air inlet to the air outlet is provided; and a chamber coupled to the elastic diaphragm.
摘要翻译： 根据各种实施例，可以提供微流体搅拌装置。 微流体搅拌装置可以包括：空气入口; 一个出风口 设置在所述空气入口和所述空气出口之间并且被配置为当从所述空气入口到所述空气出口的气流被提供时振荡的弹性隔膜; 以及耦合到弹性隔膜的腔室。
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公开(公告)号：US09933390B2
公开(公告)日：2018-04-03
申请号：US14647346
申请日：2013-12-13
发明人： Ruige Wu , Pin Chuan Chen , Zhiping Wang
IPC分类号： G01N27/447 , C12Q1/68
CPC分类号： G01N27/44739 , C12Q1/6806 , G01N27/44721 , G01N27/44726 , G01N27/44778 , G01N27/44791 , C12Q2565/629
摘要： A device for extracting at least one analyte may include: a sample reservoir configured to contain a sample comprising at least one target analyte and interfering materials; at least one extraction chamber connected to the sample reservoir; at least one porous structure lining one or more sides of the at least one extraction chamber; and a voltage source configured to provide a first voltage and a second voltage, wherein, when the first voltage is provided, the at least one target analyte and the interfering materials move towards the at least one extraction chamber or to a predetermined area from the at least one extraction chamber, wherein, when the second voltage is provided, the interfering materials pass through and exit the at least one extraction chamber, and the at least one target analyte is stopped from exiting the at least one extraction chamber by means of the at least one porous structure.
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6.发明申请MICROFLUIDIC DEVICE FOR GEL ELECTROPHORESIS AND METHOD OF MANUFACTURING THEREOF 有权用于凝胶电泳的微流体装置及其制造方法公开(公告)号：US20150260682A1
公开(公告)日：2015-09-17
申请号：US14658088
申请日：2015-03-13
发明人： Ruige WU , Zhiping Wang , Sai Kiang Lim , Yen Peng Daphne Seah
IPC分类号： G01N27/447 , B65B3/04
CPC分类号： G01N27/44791 , G01N27/44782
摘要： According to embodiments of the present invention, a microfluidic device for gel electrophoresis is provided. The microfluidic device includes a sample channel configured to receive a sample; a stacking channel comprising a preloaded stacking reagent; and a separation channel comprising a preloaded separation reagent, wherein the preloaded stacking reagent has a physical characteristic different from that of the preloaded separation reagent; and wherein the sample channel, the stacking channel and the separation channel are in fluid communication with one another. According to further embodiments of the present invention, a method of manufacturing a microfluidic device for gel electrophoresis is also provided.
摘要翻译： 根据本发明的实施例，提供了一种用于凝胶电泳的微流体装置。 微流体装置包括构造成接收样品的样品通道; 堆叠通道，包括预加载的堆叠试剂; 以及包含预加载的分离试剂的分离通道，其中所述预加载的堆叠试剂具有与所述预加载的分离试剂的物理特性不同的物理特性; 并且其中所述样品通道，所述堆叠通道和所述分离通道彼此流体连通。 根据本发明的另外的实施方案，还提供了制造用于凝胶电泳的微流体装置的方法。
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公开(公告)号：US11566212B2
公开(公告)日：2023-01-31
申请号：US16324571
申请日：2017-08-10
发明人： Paul Bigliardi , Zhiping Wang , Ruige Wu , Massimo Alberti , Bo Wu , Mei Bigliardi-Qi , Sriram Gopu , Srinivas Ramasamy , Yuri Hebert Dancik
IPC分类号： B01L7/00 , C12M3/06 , C12M3/00 , C12M1/00 , C12M1/12 , C12M1/42 , B01L3/00 , B01L9/00 , G01N33/50 , C12N5/00 , C12N5/071
摘要： The present disclosure describes a microfluidic chip for culturing and in vitro testing of 3D organotypic cultures. The tests may be performed directly on the organotypic culture in the microfluidic chip. The microfluidic chip includes at least one microfluidic unit which includes two fluidic compartments, such as upper and lower, separated by a permeable supporting structure, one or more access opening for the fluidic compartments, and a set of lids interchangeable with a set of insets. The permeable support structure serves as a support for the organotypic culture. The upper and lower compartments may include inlets and outlets which allow fluids to be perfused into the lower compartment and fluids to be perfused into the upper compartment. The access opening may be closed with a lid or accommodate an inset.
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公开(公告)号：US10024820B2
公开(公告)日：2018-07-17
申请号：US14658088
申请日：2015-03-13
发明人： Ruige Wu , Zhiping Wang , Sai Kiang Lim , Yen Peng Daphne Seah
IPC分类号： G01N27/447 , B65B3/04
摘要： According to embodiments of the present invention, a microfluidic device for gel electrophoresis is provided. The microfluidic device includes a sample channel configured to receive a sample; a stacking channel comprising a preloaded stacking reagent; and a separation channel comprising a preloaded separation reagent, wherein the preloaded stacking reagent has a physical characteristic different from that of the preloaded separation reagent; and wherein the sample channel, the stacking channel and the separation channel are in fluid communication with one another. According to further embodiments of the present invention, a method of manufacturing a microfluidic device for gel electrophoresis is also provided.
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公开(公告)号：US20150323498A1
公开(公告)日：2015-11-12
申请号：US14647346
申请日：2013-12-13
发明人： Ruige Wu , Pin Chuan Chen , Zhiping Wang
IPC分类号： G01N27/447
CPC分类号： G01N27/44739 , C12Q1/6806 , G01N27/44721 , G01N27/44726 , G01N27/44778 , G01N27/44791 , C12Q2565/629
摘要： A device for extracting at least one analyte may include: a sample reservoir configured to contain a sample comprising at least one target analyte and interfering materials; at least one extraction chamber connected to the sample reservoir; at least one porous structure lining one or more sides of the at least one extraction chamber; and a voltage source configured to provide a first voltage and a second voltage, wherein, when the first voltage is provided, the at least one target analyte and the interfering materials move towards the at least one extraction chamber or to a predetermined area from the at least one extraction chamber, wherein, when the second voltage is provided, the interfering materials pass through and exit the at least one extraction chamber, and the at least one target analyte is stopped from exiting the at least one extraction chamber by means of the at least one porous structure.
摘要翻译： 用于提取至少一种分析物的装置可以包括：样品储存器，其被配置为容纳包含至少一种目标分析物和干扰材料的样品; 至少一个提取室连接到样品储存器; 在所述至少一个提取室的一个或多个侧面上的至少一个多孔结构; 以及电压源，其被配置为提供第一电压和第二电压，其中，当提供所述第一电压时，所述至少一个目标分析物和所述干扰材料朝向所述至少一个提取室移动或者从所述至少一个提取室移动到预定区域 至少一个提取室，其中当所述第二电压被提供时，所述干扰材料通过并离开所述至少一个提取室，并且所述至少一个目标分析物被停止以通过所述至少一个提取室离开所述至少一个提取室 至少一个多孔结构。
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