公开(公告)号：US20080038803A1

公开(公告)日：2008-02-14

申请号：US11885056

申请日：2006-02-24

申请人： Akira Iwasaki ,  Motohisa Washida ,  Naoaki Taoka ,  Daisuke Moriyama ,  Junzou Hasegawa

发明人： Akira Iwasaki ,  Motohisa Washida ,  Naoaki Taoka ,  Daisuke Moriyama ,  Junzou Hasegawa

IPC分类号： C12P7/02 ,  C12N1/00

CPC分类号： C12P41/002 ,  C12N9/0008 ,  C12N9/0036 ,  C12P7/02 ,  C12P7/18

摘要： The present invention provides a method enabling convenient production of an optically active secondary alcohol useful as a pharmaceutical intermediate, particularly an optically active 1,2-diol and an optically active 2-alkanol, from an enantiomer mixture thereof. An oxidizing enzyme source having the capability of selectively oxidizing one enantiomer of secondary alcohol is allowed to act on an enantiomer mixture of secondary alcohol in the presence of a reducing enzyme source having the capability of reverse enantio-selectively reducing a ketone derivative, to convert the enantiomer mixture into a substantially single enantiomer at a theoretical percent recovery of 100%, whereby an optically active secondary alcohol is produced.

摘要翻译： 本发明提供了一种能够方便地制备可用作药物中间体，特别是光学活性的1,2-二醇和光学活性2-链烷醇的光学活性仲醇的方法。 在具有反向对映选择性还原酮衍生物的能力的还原酶源的存在下，使具有选择性氧化仲醇的对映异构体的氧化酶源作用于仲醇的对映体混合物， 对映异构体混合物变成基本上单一的对映体，理论回收率为100％，由此产生光学活性的仲醇。

公开(公告)号：US20070292923A1

公开(公告)日：2007-12-20

申请号：US11659473

申请日：2005-08-01

申请人： Akira Iwasaki ,  Motohisa Washida ,  Junzou Hasegawa

发明人： Akira Iwasaki ,  Motohisa Washida ,  Junzou Hasegawa

IPC分类号： C12P19/36 ,  C12N1/20 ,  C12N15/00 ,  C12N9/02 ,  C12N9/14

CPC分类号： C12P41/002 ,  C12P7/16 ,  C12P7/22 ,  Y02E50/10

摘要： The present invention relates to a production method of an enantiomer-enriched compound, which includes the following (1) and (2): (1) contacting an enantiomer mixture with a stereoselective dehydrogenase, for which NAD+ (nicotinamide adenine dinucleotide) is a coenzyme, thereby preferentially oxidizing one enantiomer and leaving the other enantiomer to form NADH, and (2) contacting the NADH formed by the oxidation reaction in (1) with a water-forming NADH oxidase stable under oxidative conditions to convert the NADH to NAD+. According to the present invention, in the production of an enantiomer-enriched compound from an enantiomer mixture, which uses a dehydrogenase, NAD+ can be regenerated even without addition of a stabilizer and an enantiomer-enriched compound can be obtained efficiently.

摘要翻译： 本发明涉及富含对映异构体的化合物的制备方法，其包括以下（1）和（2）：（1）使对映异构体混合物与立体选择性脱氢酶接触，其中NAD +（烟酰胺腺嘌呤二核苷酸）是辅酶 ，从而优先氧化一种对映异构体并使另一种对映异构体形成NADH，和（2）使（1）中的氧化反应形成的NADH与在氧化条件下稳定的水形式的NADH氧化酶接触以将NADH转化为NAD +。 根据本发明，在使用脱氢酶的对映异构体混合物的富含对映体的化合物的制造中，即使不添加稳定剂也能够再生NAD +，能够有效地获得富含对映体的化合物。

公开(公告)号：US20070026510A1

公开(公告)日：2007-02-01

申请号：US10544618

申请日：2004-02-13

申请人： Akira Iwasaki ,  Takehiro Matsumoto ,  Motohisa Washida ,  Hiroshi Watanabe ,  Junzou Hasegawa ,  Sakayu Shimizu

发明人： Akira Iwasaki ,  Takehiro Matsumoto ,  Motohisa Washida ,  Hiroshi Watanabe ,  Junzou Hasegawa ,  Sakayu Shimizu

IPC分类号： C12P7/50 ,  C12N1/20

CPC分类号： C12P7/40

摘要： The present invention provides an industrially advantageous process for biochemical production of glyoxylic acid from glyoxal. More specifically, the present invention provides a process for production of glyoxylic acid, which is characterized in that the process comprises allowing oxidoreductase that can convert glyoxal into glyoxylic acid, such as oxidase and dehydrogenase, to act on glyoxal, so as to convert glyoxal into glyoxylic acid.

摘要翻译： 本发明提供了从乙二醛生物化生产乙醛酸的工业上有利的方法。 更具体地说，本发明提供了一种生产乙醛酸的方法，其特征在于该方法包括允许将乙二醛转化为乙醛酸（如氧化酶和脱氢酶）的氧化还原酶作用于乙二醛，从而将乙二醛转化为 乙醛酸。

公开(公告)号：US20050170481A1

公开(公告)日：2005-08-04

申请号：US10513432

申请日：2003-05-22

申请人： Akira Iwasaki ,  Takehiko Matsumoto ,  Motohisa Washida ,  Hiroshi Watanabe ,  Sakayu Shimizu

发明人： Akira Iwasaki ,  Takehiko Matsumoto ,  Motohisa Washida ,  Hiroshi Watanabe ,  Sakayu Shimizu

IPC分类号： C12N9/04 ,  C12P7/58 ,  C12P7/50 ,  C12N1/21 ,  C12N9/02

CPC分类号： C12N9/0006 ,  C12P7/58

摘要： The present invention provides an industrially advantageous process for producing glyoxylic acid by a biochemical procedure using oxidase. In particular, the present invention provides novel (D)-2-hydroxy-acid oxidase acting on a 2-hycoxy acid to produce a corresponding 2-keto acid, and a process for producing glyoxylic acid in which the 2-hydroxy-acid oxidase, a culture solution of microorganisms capable of producing the enzyme, microorganism cells isolated from the culture solution, or a product of treatment of microorganism cells acts on glycolic acid to convert the glycolic acid to glyoxylic acid.

摘要翻译： 本发明提供了通过使用氧化酶的生物化学方法生产乙醛酸的工业上有利的方法。 特别地，本发明提供作用于2-羟基酸以产生相应的2-酮酸的新的（D）-2-羟基酸氧化酶，以及生产二羟基酸的方法，其中2-羟基酸氧化酶 ，能够生产酶的微生物培养液，从培养液中分离的微生物细胞或微生物细胞处理的产物作用于乙醇酸以将乙醇酸转化成乙醛酸。

公开(公告)号：US20130030164A1

公开(公告)日：2013-01-31

申请号：US13574458

申请日：2011-01-19

申请人： Shinichi Yoshida ,  Akira Iwasaki ,  Motohisa Washida ,  Tozo Nishiyama ,  Daisuke Moriyama ,  Naoaki Taoka

发明人： Shinichi Yoshida ,  Akira Iwasaki ,  Motohisa Washida ,  Tozo Nishiyama ,  Daisuke Moriyama ,  Naoaki Taoka

IPC分类号： C12N9/02 ,  C12N15/63 ,  C07H21/02 ,  C12N1/19 ,  C12N5/10 ,  C12P19/36 ,  C12N15/53 ,  C12N1/21

CPC分类号： C12N9/0036 ,  C12N9/0065 ,  C12P7/02 ,  C12P7/18 ,  C12P7/42 ,  C12P13/04 ,  C12P19/36 ,  C12Y106/03 ,  C12Y106/03001 ,  C12Y111/01001

摘要： Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.

摘要翻译： 来自变形链球菌的成水NADH氧化酶在工业生产实际使用的稳定性方面应进一步提高。 本发明的目的是提供通过蛋白质工程技术，通过改性水合NADH氧化酶获得的酶，其可用作由氧化还原酶催化的用于立体选择性氧化的NAD +再生系统，使得酶能够承受 长期使用而不显示其NAD +再生活性的活性降低，即稳定性提高的酶，以及提供有效生产光学活性醇或氨基酸等有用物质的方法。 本发明涉及通过适当地引入突变来提高来自变形链球菌的水形成NADH氧化酶的稳定性的酶修饰方法。

公开(公告)号：US09315782B2

公开(公告)日：2016-04-19

申请号：US13574458

申请日：2011-01-19

申请人： Shinichi Yoshida ,  Akira Iwasaki ,  Motohisa Washida ,  Tozo Nishiyama ,  Daisuke Moriyama ,  Naoaki Taoka

发明人： Shinichi Yoshida ,  Akira Iwasaki ,  Motohisa Washida ,  Tozo Nishiyama ,  Daisuke Moriyama ,  Naoaki Taoka

IPC分类号： C07H21/00 ,  C12N15/00 ,  C12N15/53 ,  C12N1/21 ,  C12N5/10 ,  C12N1/15 ,  C12N9/08 ,  C12N9/02 ,  C12P19/36 ,  C12P7/02 ,  C12P7/18 ,  C12P7/42 ,  C12P13/04

CPC分类号： C12N9/0036 ,  C12N9/0065 ,  C12P7/02 ,  C12P7/18 ,  C12P7/42 ,  C12P13/04 ,  C12P19/36 ,  C12Y106/03 ,  C12Y106/03001 ,  C12Y111/01001

摘要： Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.

摘要翻译： 来自变形链球菌的成水NADH氧化酶在工业生产实际使用的稳定性方面应进一步提高。 本发明的目的是提供通过蛋白质工程技术，通过改性水合NADH氧化酶获得的酶，其可用作由氧化还原酶催化的用于立体选择性氧化的NAD +再生系统，使得酶能够承受 长期使用而不显示其NAD +再生活性的活性降低，即稳定性提高的酶，以及提供有效生产光学活性醇或氨基酸等有用物质的方法。 本发明涉及通过适当地引入突变来提高来自变形链球菌的水形成NADH氧化酶的稳定性的酶修饰方法。

公开(公告)号：US06221638B1

公开(公告)日：2001-04-24

申请号：US09064750

申请日：1998-04-23

申请人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

发明人： Yukio Yamada ,  Akira Iwasaki ,  Noriyuki Kizaki ,  Yasuhiro Ikenaka ,  Masahiro Ogura ,  Junzo Hasegawa

IPC分类号： C12P1300

CPC分类号： C12P13/001 ,  C12N9/1096 ,  C12P13/005 ,  C12P13/008 ,  C12P13/04 ,  C12P41/006 ,  Y10S435/83

摘要： The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.

摘要翻译： 制备光学活性（R） - 氨基化合物的方法，其特征在于在酮化合物（氨基受体）的共存下通过（R） - 形式 - 特异性转氨酶的作用立体选择性地进行氨基转移的方法， 和外消旋形式的氨基化合物（氨基供体）或（R） - 形式，得到光学活性（R） - 氨基化合物。 根据本发明，可以容易地容易地制备其1-位上具有芳基等的光学活性（R） - 氨基化合物等，其通常难以制备。

公开(公告)号：US06513424B1

公开(公告)日：2003-02-04

申请号：US09647897

申请日：2000-10-06

申请人： Akira Iwasaki ,  Toshinari Sasaki ,  Shigetoshi Kashiwagi ,  Shigemi Isobe

发明人： Akira Iwasaki ,  Toshinari Sasaki ,  Shigetoshi Kashiwagi ,  Shigemi Isobe

IPC分类号： B30B1116

CPC分类号： B30B11/16 ,  B30B11/006 ,  B30B11/18 ,  B30B15/0017 ,  B30B15/0082 ,  B30B15/308

摘要： A pair of compression rollers 38a and 38b parallel with each other are provided and powder grains are supplied to a powder grain introduction/compression part 50 formed between the rollers 38a and 38b and, thereby, compression moldings of the powder grains are formed. A powder grain press/feed means 20 is provided in a front stage of the rollers 38a and 38b. The press/feed means 20 has a deaerating barrel 24 and previously presses the powder grains supplied between the rollers 38a and 38b. In side surfaces of the rollers 38a and 38b, side seals 37 are arranged with clearances 72 maintained from the rollers 38a and 38b. During pressing the powder moldings, the powder grains enter into the clearances so that closer layers are formed between the side surfaces of the compression rollers 38a and 38b and the side seals 37, thereby sealing the powder grain introduction/compression part 50. In a rear stage of the rollers 38a and 38b, a shearing device 75 is provided and shears the compression moldings formed by the rollers 38a and 38b. Torque of the shearing device 75 is detected by a torque sensor, and the compression rollers 38a and 38b and the powder grain transport means 17 are controlled in accordance with the detected torque.

摘要翻译： 设置一对彼此平行的压缩辊38a和38b，并且将粉末颗粒供给到形成在辊38a和38b之间的粉末颗粒引入/压缩部分50，从而形成粉末颗粒的压缩模制品。 在辊38a和38b的前段设置有粉粒挤压/进给装置20。 压榨/进料装置20具有脱气筒24并预先挤压供应在辊38a和38b之间的粉末颗粒。 在辊38a和38b的侧表面中，侧密封件37布置有从辊38a和38b保持的间隙72。 在挤压粉末成型体期间，粉粒进入间隙，使得在压缩辊38a和38b的侧表面与侧密封件37之间形成更近的层，从而密封粉粒引入/压缩部50.在后部 辊38a和38b的阶段，设置有剪切装置75，并且剪切由辊38a和38b形成的压缩模制品。 通过转矩传感器检测剪切装置75的扭矩，根据检测的转矩来控制压缩辊38a，38b和粉粒传送装置17。

公开(公告)号：US06291557B1

公开(公告)日：2001-09-18

申请号：US09695975

申请日：2000-10-26

申请人： Kyoichi Yamamoto ,  Kojun Utaka ,  Hisashi Takada ,  Akira Iwasaki

发明人： Kyoichi Yamamoto ,  Kojun Utaka ,  Hisashi Takada ,  Akira Iwasaki

IPC分类号： C08K310

CPC分类号： C09J163/00 ,  C08G59/4292

摘要： The invention provides an alkali-soluble adhesive agent characterized by comprising an alkali-soluble resin that is a reaction product (C) obtained by allowing a polycarboxylic acid and/or a polycarboxylic anhydride having two or more carboxyl groups to react with a reaction product of a compound (A) having at least one epoxy group with a monocarboxylic acid compound (B). Preferably, the alkali-soluble resin is used in combination with a ketone organic solvent, optionally with at least one member selected from the group consisting of a melt viscosity reducing agent, a surface active agent and a plasticizer. The alkali-soluble adhesive agent can be removed by an alkali aqueous solution without recourse to any organic solvent, and is of an environmental protection-conscious type with improvements in adhesion, adhesion strength, coatability, surface smoothness, softening point controllability, melt viscosity controllability and spread controllability.

摘要翻译： 本发明提供一种碱溶性粘合剂，其特征在于包含碱溶性树脂，其为通过使多羧酸和/或具有两个或更多个羧基的多元羧酸酐与下述反应产物反应获得的反应产物（C） 具有至少一个环氧基的化合物（A）与单羧酸化合物（B）。 优选地，碱溶性树脂与酮有机溶剂组合使用，任选地与选自熔体粘度降低剂，表面活性剂和增塑剂中的至少一种组合使用。 碱溶性粘合剂可以通过碱水溶液除去而不用任何有机溶剂，并且具有环保意识型，其粘合性，粘合强度，可涂布性，表面平滑性，软化点可控性，熔体粘度可控性 并传播可控性。

公开(公告)号：US5906704A

公开(公告)日：1999-05-25

申请号：US937463

申请日：1997-09-25

申请人： Kazushige Matsuura ,  Akira Iwasaki ,  Hideki Ohmori

发明人： Kazushige Matsuura ,  Akira Iwasaki ,  Hideki Ohmori

IPC分类号： A41D27/06 ,  B29C41/18 ,  C08G18/10 ,  C08G18/80 ,  C08K5/16 ,  C08K5/21 ,  C08L75/04 ,  C09J175/04 ,  C09K3/00 ,  C08G18/40 ,  C08G18/66 ,  C08K5/205

CPC分类号： C08G18/10 ,  C09J175/04 ,  C08G2170/20

摘要： The present invention provides a hot-melt resin composition which comprises 1 to 30% by weight of a compound represented by the general formula (1)Q-(X-A)n (1)(wherein X is a urethane or urea group, Q is a group with a valence of n, A is a univalent group, one of Q and A being an isocyanate residue and the other being an amine or alcohol residue, and n is an integer of 1 to 3) and 70 to 99% by weight of a thermoplastic polyurethane resin (B); a method of producing a powder form of said hot-melt resin composition; and a hot-melt resin powder composition which comprises said powder-form hot-melt resin composition.

摘要翻译： 本发明提供一种热熔树脂组合物，其包含1至30重量％的由通式（1）表示的化合物Q-（XA）n（1）（其中X为氨基甲酸酯或脲基，Q为 化合价为n的基团，A为一价基团，Q和A之一为异氰酸酯残基，另一个为胺或醇残基，n为1〜3的整数），70〜99重量％ 的热塑性聚氨酯树脂（B）; 一种生产所述热熔树脂组合物的粉末形式的方法; 和包含所述粉末状热熔树脂组合物的热熔树脂粉末组合物。