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1.发明公开公开(公告)号：US20240012002A1
公开(公告)日：2024-01-11
申请号：US18335713
申请日：2023-06-15
CPC分类号： G01N33/6851 , G01N1/28 , G01N33/5304 , B01L3/502738 , G01N2001/284 , B01L2300/0816 , B01L2300/049 , B01L2400/0638 , G01N2333/46
摘要： Described herein are systems and methods for a microfluidic immunoassay for in situ mass spectrometry analysis of intracellular protein biomarkers in tissue. In some embodiments, the tissue may comprise human brain tissue. In some embodiments, the protein biomarkers may comprise Aβ species comprising monomers and oligomers of Aβ1-42, Aβ1-40, Aβ1-39, Aβ2-43, or combinations thereof. In some embodiments, the systems and methods may comprise laser capture microdissection (LCM) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.
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公开(公告)号：US20230243765A1
公开(公告)日：2023-08-03
申请号：US18295761
申请日：2023-04-04
申请人： Alexandra Ros , Daihyun Kim , Diandra Doppler , Jorvani Cruz Villarreal , Richard Kirian , Reza Nazari , Sahir Gandhi
发明人： Alexandra Ros , Daihyun Kim , Diandra Doppler , Jorvani Cruz Villarreal , Richard Kirian , Reza Nazari , Sahir Gandhi
IPC分类号： G01N23/20025 , G01N23/201 , B01L3/02 , G01N35/10 , B01L3/00
CPC分类号： G01N23/20025 , G01N23/201 , B01L3/0241 , G01N35/1016 , B01L3/502715 , G01N2223/30 , G01N2223/604 , G01N2223/612 , G01N2223/637 , G01N2223/1016 , G01N2223/203 , G01N2223/054 , G01N2035/1034 , B01L2300/0645
摘要： A single-piece hybrid droplet generator and nozzle component for serial crystallography. The single-piece hybrid droplet generator component including an internally-formed droplet-generation channel, an internally-formed sample channel, a nozzle, and a pair of electrode chambers. The droplet-generation channel extends from a first fluid inlet opening to the nozzle. The sample channel extends from a second fluid inlet opening to the droplet-generation channel and joins the droplet-generation channel at a junction. The nozzle is configured to eject a stream of segmented aqueous droplets in a carrier fluid from the droplet-generation channel through a nozzle opening of the single-piece component. The pair of electrode chambers are positioned adjacent to the droplet-generation channel near the junction between the droplet-generation channel and the sample channel. The timing of sample droplets in the stream of fluid ejected through the nozzle is controlled by applying a triggering signal to electrodes positioned in the electrode chambers of the single-piece component.
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公开(公告)号：US11318487B2
公开(公告)日：2022-05-03
申请号：US15930239
申请日：2020-05-12
申请人： Alexandra Ros , Daihyun Kim , Austin Echelmeier , Jorvani Cruz Villarreal , Diandra Doppler , Richard Kirian , Reza Nazari
发明人： Alexandra Ros , Daihyun Kim , Austin Echelmeier , Jorvani Cruz Villarreal , Diandra Doppler , Richard Kirian , Reza Nazari
IPC分类号： B05B7/04 , G01N23/20008
摘要： Systems and methods for performing serial crystallography by providing an aqueous suspension of a crystal sample to a T-junction at a first flow rate and providing an immiscible oil fluid to the T-junction at a second flow rate. The aqueous suspension and the oil are combined at the T-junction to produce a co-flow output fluid including a parallel co-flow of the aqueous suspension and the oil in the same output channel. The co-flow output fluid is ejected as a jet stream through a nozzle and the sample flow rate in the crystal sample in the jet stream is adjusted by adjusting the first flow rate of the aqueous suspension and the second flow rate of the oil. By combining the aqueous sample and the oil in this manner, the output of the jet stream can be regulated for compatibility with X-ray free electron laser serial crystallography.
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公开(公告)号：US11624718B2
公开(公告)日：2023-04-11
申请号：US15930313
申请日：2020-05-12
申请人： Alexandra Ros , Daihyun Kim , Diandra Doppler , Jorvani Cruz Villarreal , Richard Kirian , Reza Nazari , Sahir Gandhi
发明人： Alexandra Ros , Daihyun Kim , Diandra Doppler , Jorvani Cruz Villarreal , Richard Kirian , Reza Nazari , Sahir Gandhi
IPC分类号： B01L3/00 , G01N23/20025 , G01N23/201 , B01L3/02 , G01N35/10 , G01N23/20
摘要： A single-piece hybrid droplet generator and nozzle component for serial crystallography. The single-piece hybrid droplet generator component including an internally-formed droplet-generation channel, an internally-formed sample channel, a nozzle, and a pair of electrode chambers. The droplet-generation channel extends from a first fluid inlet opening to the nozzle. The sample channel extends from a second fluid inlet opening to the droplet-generation channel and joins the droplet-generation channel at a junction. The nozzle is configured to eject a stream of segmented aqueous droplets in a carrier fluid from the droplet-generation channel through a nozzle opening of the single-piece component. The pair of electrode chambers are positioned adjacent to the droplet-generation channel near the junction between the droplet-generation channel and the sample channel. The timing of sample droplets in the stream of fluid ejected through the nozzle is controlled by applying a triggering signal to electrodes positioned in the electrode chambers of the single-piece component.
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公开(公告)号：US11649173B2
公开(公告)日：2023-05-16
申请号：US17237836
申请日：2021-04-22
申请人： Ana Egatz-Gomez , Alexandra Ros
发明人： Ana Egatz-Gomez , Alexandra Ros
IPC分类号： C01F17/36 , B01J19/12 , B01J19/00 , C09K11/02 , C01F17/265 , C09K11/77 , B82Y30/00 , B82Y40/00
CPC分类号： C01F17/36 , B01J19/0013 , B01J19/126 , C01F17/265 , C09K11/025 , C09K11/7773 , B01J2219/00033 , B01J2219/00141 , B01J2219/00162 , B01J2219/00166 , B82Y30/00 , B82Y40/00 , C01P2004/38 , C01P2004/64 , C01P2006/60
摘要： Synthesizing upconverting nanoparticles includes heating a precursor solution comprising one or more rare earth salts, an alkali metal salt or alkaline earth salt, and a solvent comprising a plasticizer in a microwave reactor to yield a product mixture, and cooling the product mixture to yield the upconverting nanoparticles. Core-shell upconverting nanoparticles are synthesized by combining the upconverting nanoparticles with a precursor solution comprising one or more rare earth salts, an alkali metal salt or alkaline earth salt, and a solvent comprising a plasticizer to yield a nanoparticle mixture, heating the nanoparticle mixture in a microwave reactor to yield a product mixture, and cooling the product mixture to yield the core-shell upconverting nanoparticles.
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公开(公告)号：US20220112079A1
公开(公告)日：2022-04-14
申请号：US17497675
申请日：2021-10-08
申请人： Alexandra Ros , John Spence , Diandra Doppler , Garrett Nelson , Richard Kirian , Reza Nazari , Ana Egatz-Gomez , Mukul Sonker , Mohammad Rabbani
发明人： Alexandra Ros , John Spence , Diandra Doppler , Garrett Nelson , Richard Kirian , Reza Nazari , Ana Egatz-Gomez , Mukul Sonker , Mohammad Rabbani
摘要： A microfluidic device for use in a serial crystallography apparatus includes a nozzle having an inlet, an outlet, and a first snap engagement feature. The microfluidic device further includes a fiber holder having an outlet and a second snap engagement feature. The first snap engagement feature is configured to engage the second snap engagement feature to removably couple the nozzle to the fiber holder. The outlet of the fiber holder is aligned with the inlet of the nozzle when the first snap engagement feature is coupled to the second snap engagement feature.
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公开(公告)号：US20230242412A1
公开(公告)日：2023-08-03
申请号：US18296501
申请日：2023-04-06
申请人： Ana Egatz-Gomez , Alexandra Ros
发明人： Ana Egatz-Gomez , Alexandra Ros
CPC分类号： C01F17/36 , B01J19/126 , B01J19/0013 , C09K11/025 , C01F17/265 , C09K11/7773 , B82Y30/00
摘要： Synthesizing upconverting nanoparticles includes heating a precursor solution comprising one or more rare earth salts, an alkali metal salt or alkaline earth salt, and a solvent comprising a plasticizer in a microwave reactor to yield a product mixture, and cooling the product mixture to yield the upconverting nanoparticles. Core-shell upconverting nanoparticles are synthesized by combining the upconverting nanoparticles with a precursor solution comprising one or more rare earth salts, an alkali metal salt or alkaline earth salt, and a solvent comprising a plasticizer to yield a nanoparticle mixture, heating the nanoparticle mixture in a microwave reactor to yield a product mixture, and cooling the product mixture to yield the core-shell upconverting nanoparticles.
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公开(公告)号：US20210332294A1
公开(公告)日：2021-10-28
申请号：US17237836
申请日：2021-04-22
申请人： Ana Egatz-Gomez , Alexandra Ros
发明人： Ana Egatz-Gomez , Alexandra Ros
IPC分类号： C09K11/77 , B01J19/12 , B01J19/00 , C09K11/02 , C01F17/265
摘要： Synthesizing upconverting nanoparticles includes heating a precursor solution comprising one or more rare earth salts, an alkali metal salt or alkaline earth salt, and a solvent comprising a plasticizer in a microwave reactor to yield a product mixture, and cooling the product mixture to yield the upconverting nanoparticles. Core-shell upconverting nanoparticles are synthesized by combining the upconverting nanoparticles with a precursor solution comprising one or more rare earth salts, an alkali metal salt or alkaline earth salt, and a solvent comprising a plasticizer to yield a nanoparticle mixture, heating the nanoparticle mixture in a microwave reactor to yield a product mixture, and cooling the product mixture to yield the core-shell upconverting nanoparticles.
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公开(公告)号：US20240216914A1
公开(公告)日：2024-07-04
申请号：US18603030
申请日：2024-03-12
申请人： Alexandra Ros , Daihyun Kim , Jinghui Luo
发明人： Alexandra Ros , Daihyun Kim , Jinghui Luo
CPC分类号： B01L3/502715 , B01L3/502753 , B01L3/502761 , B03C5/005 , B03C5/026 , B03C5/028 , B01L3/50273 , B01L2200/0647 , B01L2200/0652 , B01L2300/0816 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/086 , B03C2201/26 , G01N2001/4038 , G01N2015/0038
摘要： Sub-micrometer bioparticles are separated by size in a microfluidic channel utilizing a ratchet migration mechanism. A structure within the microfluidic channel includes an array of micro-posts arranged in laterally shifted rows. Reservoirs are disposed at each end of the microfluidic channel. A biased AC potential is applied across the channel via electrodes immersed into fluid in each of the reservoirs to induce a non-uniform electric field through the microfluidic channel. The applied potential comprises a first waveform with a first frequency that induces electro-kinetic flow of sub-micrometer bioparticles in the microfluidic channel, and an intermittent superimposed second waveform with a higher frequency. The second waveform selectively induces a dielectrophoretic trapping force to selectively impart ratchet migration based on particle size for separating the sub-micrometer bioparticles by size in the microfluidic channel.
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公开(公告)号：US11944967B2
公开(公告)日：2024-04-02
申请号：US17524976
申请日：2021-11-12
申请人： Alexandra Ros , Daihyun Kim , Jinghui Luo
发明人： Alexandra Ros , Daihyun Kim , Jinghui Luo
CPC分类号： B01L3/502715 , B01L3/502753 , B01L3/502761 , B03C5/005 , B03C5/026 , B03C5/028 , B01L3/50273 , B01L2200/0647 , B01L2200/0652 , B01L2300/0816 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/086 , B03C2201/26 , G01N2001/4038 , G01N2015/0038
摘要： Sub-micrometer bioparticles are separated by size in a microfluidic channel utilizing a ratchet migration mechanism. A structure within the microfluidic channel includes an array of micro-posts arranged in laterally shifted rows. Reservoirs are disposed at each end of the microfluidic channel. A biased AC potential is applied across the channel via electrodes immersed into fluid in each of the reservoirs to induce a non-uniform electric field through the microfluidic channel. The applied potential comprises a first waveform with a first frequency that induces electro-kinetic flow of sub-micrometer bioparticles in the microfluidic channel, and an intermittent superimposed second waveform with a higher frequency. The second waveform selectively induces a dielectrophoretic trapping force to selectively impart ratchet migration based on particle size for separating the sub-micrometer bioparticles by size in the microfluidic channel.
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