公开(公告)号：US20080038287A1

公开(公告)日：2008-02-14

申请号：US11614716

申请日：2006-12-21

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire Fraser ,  Steven Gill

IPC分类号： A61K39/00 ,  A61P43/00 ,  C07K14/00 ,  C07K16/18 ,  C12N15/00 ,  C12P21/04 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: —providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —screening said at least one expression library with said antibody preparation, —identifying antigens which bind in said screening to antibodies in said antibody preparation, —screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and —optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选，用来自具有抗体a的个体的来自个体血清的各抗体制剂筛选鉴定的抗原 所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主，鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原与所述单个抗体制剂的相关部分结合来自所述 单独血清，并且通过化学或重组方法选择性分离所述超免疫血清反应性抗原并产生所述超免疫血清反应性抗原。

公开(公告)号：US20050037444A1

公开(公告)日：2005-02-17

申请号：US10470048

申请日：2002-01-21

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire Fraser ,  Steven Gill

IPC分类号： G01N33/50 ,  A61K39/00 ,  A61K39/39 ,  A61K39/395 ,  A61P31/10 ,  B64C9/02 ,  C07K14/195 ,  C07K14/31 ,  C07K16/12 ,  C12N15/02 ,  C12N15/09 ,  C12N15/10 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  C40B40/02 ,  G01N33/15 ,  G01N33/53 ,  G01N33/574 ,  G01N33/554 ,  G01N33/569

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps:—providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—screening said at least one expression library with said antibody preparation,—identifying antigens which bind in said screening to antibodies in said antibody preparation,—screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and—optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选，并用来自具有抗体的个体的来自个体血清的单独抗体制剂筛选鉴定的抗原 t表示特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主， - 识别所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原与所述单个抗体制剂的相关部分结合来自所述 单独血清和任选分离所述超免疫血清反应性抗原，并通过化学或重组方法产生所述超免疫血清反应性抗原。

公开(公告)号：US20130034575A1

公开(公告)日：2013-02-07

申请号：US13613761

申请日：2012-09-13

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： A61K39/00 ,  C07K16/00 ,  C07K7/08 ,  C07K14/00 ,  C07K7/06

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to auto-immunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: providing an antibody preparation, providing at least one expression library, identifying antigens which bind, screening the identified antigens, identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了一种鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型的疫苗 其特征在于以下步骤：提供抗体制剂，提供至少一种表达文库，鉴定结合筛选鉴定的抗原的抗原，鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，以及哪些 超免疫血清反应性抗原结合所述单独抗体的相关部分，任选分离所述超免疫血清反应性抗原，并通过化学或重组方法产生所述超免疫血清反应性抗原。

公开(公告)号：US08715688B2

公开(公告)日：2014-05-06

申请号：US13613761

申请日：2012-09-13

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： A61K39/085

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to auto-immunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: providing an antibody preparation, providing at least one expression library, identifying antigens which bind, screening the identified antigens, identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了一种鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型的疫苗 其特征在于以下步骤：提供抗体制剂，提供至少一种表达文库，鉴定结合筛选鉴定的抗原的抗原，鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，以及哪些 超免疫血清反应性抗原结合所述单独抗体的相关部分，任选分离所述超免疫血清反应性抗原，并通过化学或重组方法产生所述超免疫血清反应性抗原。

公开(公告)号：US07771728B2

公开(公告)日：2010-08-10

申请号：US11614716

申请日：2006-12-21

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： A61K39/085

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: —providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —screening said at least one expression library with said antibody preparation, —identifying antigens which bind in said screening to antibodies in said antibody preparation, —screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and —optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选， - 用来自个体的个体血清筛选鉴定的抗原与单独的抗体制剂 针对所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的抗体， - 鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原结合所述单个抗体制剂的相关部分 所述个体血清和 - 通过化学或重组方法选择性分离所述超免疫血清反应性抗原并产生所述超免疫血清反应性抗原。

公开(公告)号：US07968297B2

公开(公告)日：2011-06-28

申请号：US10470048

申请日：2002-01-21

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitte Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： G01N33/53

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: —providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —screening said at least one expression library with said antibody preparation, —identifying antigens which bind in said screening to antibodies in said antibody preparation, —screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, —identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and —optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选， - 用来自个体的个体血清筛选鉴定的抗原与单独的抗体制剂 针对所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的抗体， - 鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原结合所述单个抗体制剂的相关部分 所述个体血清和 - 通过化学或重组方法选择性分离所述超免疫血清反应性抗原并产生所述超免疫血清反应性抗原。

公开(公告)号：US08323660B2

公开(公告)日：2012-12-04

申请号：US12853074

申请日：2010-08-09

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitre Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitre Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： A61K39/085

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps:—providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—screening said at least one expression library with said antibody preparation,—identifying antigens which bind in said screening to antibodies in said antibody preparation,—screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and—optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清提供抗体制剂 易于自身免疫，提供易于自身免疫的所述特异性病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一种表达文库， - 鉴定结合于 对所述抗体制剂中的抗体进行筛选，用来自具有抗体的个体的来自个体血清的单独抗体制剂筛选鉴定的抗原 针对所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主， - 鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原与所述单个抗体制剂的相关部分结合 所述个体血清和任选地分离所述超免疫血清反应性抗原并通过化学或重组方法产生所述超免疫血清反应性抗原。

公开(公告)号：US20110020402A1

公开(公告)日：2011-01-27

申请号：US12853074

申请日：2010-08-09

申请人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitre Tempelmaier ,  Claire M. Fraser ,  Steven Gill

发明人： Andreas Meinke ,  Eszter Nagy ,  Uwe Von Ahsen ,  Christoph Klade ,  Tamas Henics ,  Wolfgang Zauner ,  Duc Bui Minh ,  Oresta Vytvytska ,  Hildegard Etz ,  Agnieszka Dryla ,  Thomas Weichhart ,  Martin Hafner ,  Brigitre Tempelmaier ,  Claire M. Fraser ,  Steven Gill

IPC分类号： A61K39/085 ,  C07K7/06 ,  C07K2/00 ,  A61K39/00 ,  C07K16/00 ,  C07K16/12 ,  G01N33/53 ,  A61P31/04 ,  A61P37/04

CPC分类号： C40B40/02 ,  A61K39/00 ,  C07K14/195 ,  C07K14/31 ,  C07K16/1271 ,  C12N15/1037 ,  C12N15/1041 ,  C12Q1/6806 ,  Y02A50/401 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/52 ,  Y02A50/54 ,  Y02A50/57 ,  C12Q2527/125 ,  C12Q2521/537

摘要： Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps:—providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogren, tumor, allergen or tissue or host prone to auto-immunity,—providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—screening said at least one expression library with said antibody preparation, identifying antigens which bind in said screening to antibodies in said antibody preparation, —screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity,—identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and—optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.

摘要翻译： 描述了用于鉴定，分离和产生来自特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主的超免疫血清反应性抗原的方法，所述抗原适用于给定类型动物的疫苗 其特征在于以下步骤： - 从所述给定类型的动物的血浆池或从具有针对所述特异性病原体，肿瘤，变应原或组织或宿主的抗体的人血浆池或个体血清中提供抗体制剂 易于自身免疫， - 提供易于自身免疫的所述特定病原体，肿瘤，变应原或组织或宿主的至少一个表达文库， - 用所述抗体制剂筛选所述至少一个表达文库，鉴定结合所述抗体的抗原 对所述抗体制剂中的抗体进行筛选，从具有抗体的个体的个体血清中筛选鉴别的抗原 针对所述特异性病原体，肿瘤，变应原或易于自身免疫的组织或宿主， - 鉴定所述鉴定的抗原的超免疫血清反应性抗原部分，哪些超免疫血清反应性抗原与所述单个抗体制剂的相关部分结合 所述个体血清和任选地分离所述超免疫血清反应性抗原并通过化学或重组方法产生所述超免疫血清反应性抗原。

公开(公告)号：US06392520B1

公开(公告)日：2002-05-21

申请号：US09486046

申请日：2000-05-15

申请人： Karl Hofmann ,  Martin Hafner ,  Roland Zucker

发明人： Karl Hofmann ,  Martin Hafner ,  Roland Zucker

IPC分类号： H01F2729

CPC分类号： G01D11/245

摘要： A current coil for use in a needle motion sensor for a fuel injection valve, having a coil body, which is provided with a metallic coil core, wherein connecting contact wires for the coil winding are held pressed against the coil body in formed grooves of the coil body.

摘要翻译： 一种用于燃料喷射阀的针运动传感器的电流线圈，具有线圈体，其具有金属线圈芯，其中用于线圈绕组的连接接触线在所述线圈体的形成的凹槽中被压靠在线圈体上 线圈体。

公开(公告)号：US20080120733A1

公开(公告)日：2008-05-22

申请号：US11629709

申请日：2005-06-16

申请人： Martin Hafner ,  Karina Nawrath ,  Werner Muller

发明人： Martin Hafner ,  Karina Nawrath ,  Werner Muller

IPC分类号： A01K67/027 ,  C12N15/87 ,  C12N5/02 ,  C12N5/00 ,  C12N5/04 ,  A01H1/00 ,  C12N15/00

CPC分类号： C12N15/8218 ,  C12N15/63 ,  C12N15/8209 ,  C12N15/821

摘要： The invention relates to a method for the production of a eukaryotic cell selectable by inactivation or reduction of an endogenous gene function, comprising the steps of (a) introduction of one or more vectors into the cell and (b) expression of a siRNA and preferably shRNA coded by the one or more vectors, directed against an endogenous selectable gene and inactivating same, said siRNA or shRNA being the transcription product of an RNAi selection cassette, the selection cassette comprising a section of at least 19 nucleotides of the transcribed region of the gene, said selection being operatively linked to a promoter and a transcription termination signal. Furthermore, the invention relates to a eukaryotic cell comprising an RNAi selection cassette which is directed against an endogenous selectable gene and inactivates the function of said gene; wherein the RNAi selection cassette comprises a section of the gene with a length of at least 19 nucleotides which is operatively linked to a promoter and a transcription termination signal. Moreover, the invention relates to methods for the production of a transgenic mammal, comprising the steps: (a) injection of the embryonic stem cell according to the invention or of an embryonic stem cell selected according to the method of the invention in blastocysts of a mammal, (b) transfer of the blastocysts into the uterus of a mammal, and (c) carrying the transgenic mammal to full term. Finally, the invention relates to a method for the production of a transgenic plant.

摘要翻译： 本发明涉及通过灭活或还原内源基因功能来选择性地生产真核细胞的方法，其包括以下步骤：（a）将一种或多种载体导入细胞，和（b）表达siRNA，优选 由一个或多个载体编码的针对内源可选择基因并使其失活的shRNA，所述siRNA或shRNA是RNAi选择盒的转录产物，所述选择盒包含至少19个核苷酸的转录区的部分 基因，所述选择可操作地连接到启动子和转录终止信号。 此外，本发明涉及包含针对内源可选择基因并使所述基因的功能失活的RNAi选择盒的真核细胞; 其中所述RNAi选择盒包含长度为至少19个核苷酸的基因的一部分，其与启动子和转录终止信号有效连接。 此外，本发明涉及生产转基因哺乳动物的方法，其包括以下步骤：（a）根据本发明的胚胎干细胞或根据本发明的方法选择的胚胎干细胞在囊胚中的注射 哺乳动物，（b）将囊胚转移到哺乳动物的子宫中，和（c）将转基因哺乳动物全部携带。 最后，本发明涉及生产转基因植物的方法。