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    FIBROUS SUBSTRATES FOR CELL PROPAGATION AND DIFFERENTIATION
    2.
    发明申请
    FIBROUS SUBSTRATES FOR CELL PROPAGATION AND DIFFERENTIATION 审中-公开
    纤维基质用于细胞传播和分化

    公开(公告)号：US20130295637A1

    公开(公告)日：2013-11-07

    申请号：US13814501

    申请日：2011-08-05

    申请人： Hongfang Lu ,  Andrew Chwee Aun Wan ,  Jackie Y. Ying ,  Karthikeyan Narayanan

    发明人： Hongfang Lu ,  Andrew Chwee Aun Wan ,  Jackie Y. Ying ,  Karthikeyan Narayanan

    IPC分类号： C12N11/04

    CPC分类号： C12N11/04 ,  C12N5/0012 ,  C12N5/0606 ,  C12N2533/72 ,  C12N2533/74

    摘要： The present invention relates to a method of releasably encapsulating pluripotent embryonic stem cells in a degradable continuous polyionic fiber for tissue culture, wherein the encapsulated embryonic stem cells are able to maintain a pluripotent phenotype in tissue culture; the method comprising (a) contacting an aqueous solution of a polyanion with an aqueous solution of a polycation to form an interface between the aqueous solution of polyanion and the aqueous solution of polycation, and wherein the aqueous solution of polyanion or the aqueous solution of polycation or both the aqueous solution of polyanion and the aqueous solution of polycation comprises a suspension of pluripotent embryonic stem cells; (b) drawing a continuous polyionic fiber which comprises encapsulated pluripotent embryonic stem cells from the interface; (c) passing the continuous polyionic fiber comprising encapsulated pluripotent embryonic stem cells in a continuous process through a solution which reduces secondary complexation of the components of the polyionic fiber.

    摘要翻译： 本发明涉及一种将多能胚胎干细胞可释放地包封在用于组织培养的可降解连续聚离子纤维中的方法，其中包封的胚胎干细胞能够在组织培养中保持多能表型; 该方法包括（a）将聚阴离子的水溶液与聚阳离子水溶液接触以形成聚阴离子水溶液与聚阳离子水溶液之间的界面，并且其中聚阴离子水溶液或聚阳离子水溶液 或聚阴离子水溶液和聚阳离子水溶液两者均包含多能胚胎干细胞的悬浮液; （b）从界面绘制包含多能胚胎干细胞的连续聚离子纤维; （c）使包含多能胚胎干细胞的连续聚离子纤维在连续过程中通过减少聚离子纤维组分的次级络合的溶液。

    MICROFABRICATED SCAFFOLD STRUCTURES
    5.
    发明申请
    MICROFABRICATED SCAFFOLD STRUCTURES 审中-公开
    微结构SCAFFOLD结构

    公开(公告)号：US20130203146A1

    公开(公告)日：2013-08-08

    申请号：US13813936

    申请日：2011-08-03

    申请人： Jackie Y. Ying ,  Tseng-Ming Hsieh ,  Andrew Chwee Aun Wan

    发明人： Jackie Y. Ying ,  Tseng-Ming Hsieh ,  Andrew Chwee Aun Wan

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0062 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/62 ,  B33Y80/00

    摘要： The present invention relates to a method for producing a three-dimensional scaffold construct comprising encapsulated cells, the method comprising: (a) providing a solution comprising cells, a photoinitiator, and a plurality of units capable of forming polymer chains; (b) providing a photolithography instrument comprising a two-photon laser; and (c) using the instrument to apply the laser to the solution to activate the photoinitiator thereby facilitating polymerisation of said units to form polymer chains, and, cross-linking of the polymer chains; wherein the laser is applied to the solution in three-dimensions in a pre-defined pattern to assemble said construct, and said cells are encapsulated within the assembled construct.

    摘要翻译： 本发明涉及一种生产包含胶囊细胞的三维支架结构的方法，该方法包括：（a）提供包含细胞，光引发剂和能够形成聚合物链的多个单元的溶液; （b）提供包括双光子激光器的光刻仪器; 和（c）使用该仪器将激光施加到溶液中以激活光引发剂，从而促进所述单元的聚合以形成聚合物链，以及聚合物链的交联; 其中激光以预定图案以三维施加到溶液以组装所述构建体，并且所述细胞被包封在组装的构建体内。

    CONJUGATE OF MAGNETIC PARTICLE AND SURFACE MODIFIER LINKED THROUGH CLEAVABLE PEPTIDE BOND
    6.
    发明申请
    CONJUGATE OF MAGNETIC PARTICLE AND SURFACE MODIFIER LINKED THROUGH CLEAVABLE PEPTIDE BOND 审中-公开
    通过可清除的肽结合连接的磁性颗粒和表面改性剂的结合

    公开(公告)号：US20130023024A1

    公开(公告)日：2013-01-24

    申请号：US13637524

    申请日：2011-03-31

    申请人： Jackie Y. Ying ,  Karthikeyan Narayanan ,  Alex Wei Haw Lin ,  Andrew Chwee Aun Wan ,  Yuangang Zheng ,  Nandanan Erathodiyil

    发明人： Jackie Y. Ying ,  Karthikeyan Narayanan ,  Alex Wei Haw Lin ,  Andrew Chwee Aun Wan ,  Yuangang Zheng ,  Nandanan Erathodiyil

    IPC分类号： C12N13/00 ,  C12N5/071 ,  C07K7/06 ,  B82Y99/00

    CPC分类号： B82Y5/00 ,  G01N33/54326 ,  G01N33/56966

    摘要： A conjugate is provided for cell processing, which comprises a magnetic particle and a surface modifier having specific affinity to a target cell. The particle and modifier are linked through a cleavable peptide bond. In a method of cell processing, the conjugate is attached to a target cell; the target cell attached to the conjugate is subject to magnetic processing; the peptide bond is cleaved to separate the processed target cell from the magnetic particle; the target cell separated from the magnetic particle is attached to a substrate. The magnetic particle may include an iron oxide, and the surface modifier may include a glucosamine. The particle and modifier may be linked by a linker comprising a protease recognition site and a peptide bond. The linker links the surface modifier to the particle, and cleavage of the peptide bond is catalyzed by a specific protease that recognizes the protease recognition site.

    摘要翻译： 提供了用于细胞处理的缀合物，其包含对靶细胞具有特异性亲和力的磁性颗粒和表面改性剂。 颗粒和改性剂通过可切割的肽键连接。 在细胞处理方法中，缀合物附着于靶细胞; 附着于缀合物的靶细胞进行磁加工; 肽键被切割以将加工的靶细胞与磁性颗粒分离; 从磁性颗粒分离的靶细胞附着于基材。 磁性颗粒可以包括氧化铁，并且表面改性剂可以包括葡糖胺。 颗粒和改性剂可以通过包含蛋白酶识别位点和肽键的接头连接。 接头将表面改性剂连接到颗粒上，肽键的切割由识别蛋白酶识别位点的特异性蛋白酶催化。

    Method of preparing water swellable gel from chitin
    9.
    发明授权
    Method of preparing water swellable gel from chitin 失效
    从几丁质制备水溶胀凝胶的方法

    公开(公告)号：US6025479A

    公开(公告)日：2000-02-15

    申请号：US899164

    申请日：1997-07-23

    申请人： Eugene Khor ,  Andrew Chwee Aun Wan ,  Garth Winton Hastings

    发明人： Eugene Khor ,  Andrew Chwee Aun Wan ,  Garth Winton Hastings

    IPC分类号： C08B37/08 ,  C08L5/08 ,  C08B15/00

    CPC分类号： C08B37/003 ,  C08L5/08

    摘要： This invention relates to a method of preparing a form of chitin that has consistent shape in the dry form, is treated chemically such that it will swell when exposed to water to form a gel. The gel can be dried to give the dry form. The gel formation upon absorption/swelling in the presence of water and drying to give the dried form is reversible. The extent of swelling can be varied by suitably varying the chemical treatment.

    摘要翻译： 本发明涉及一种制备具有干燥形式一致形状的壳多糖形式的方法，化学处理使其在暴露于水中时会溶胀以形成凝胶。 可以将凝胶干燥以得到干燥形式。 在水的存在下吸收/溶胀时的凝胶形成并干燥得到干燥形式是可逆的。 膨胀的程度可以通过适当地改变化学处理来改变。