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    METHOD FOR IMMOBILIZING NUCLEIC ACIDS ON A SUPPORT
    5.
    发明申请
    METHOD FOR IMMOBILIZING NUCLEIC ACIDS ON A SUPPORT 有权
    用于在支持物上固定核酸的方法

    公开(公告)号：US20110319275A1

    公开(公告)日：2011-12-29

    申请号：US13121206

    申请日：2009-09-22

    申请人： Anke Pierik ,  Hendrik Roelof Stapert

    发明人： Anke Pierik ,  Hendrik Roelof Stapert

    IPC分类号： C40B20/08 ,  C40B50/18 ,  C40B40/06

    CPC分类号： C12Q1/6837 ,  C12Q2525/173

    摘要： The present invention relates to a method for immobilizing nucleic acids on a support, comprising the provision of a nucleic acid with a stretch of nucleotides of only one basetype and the immobilization of said nucleic acid on a support by crosslinking by light, wherein said crosslinking by light is performed at a wavelength of about 300-500 nm, preferably at a wavelength of 365 nm. The present invention further relates to a method for the analysis of nucleic acids immobilized according to the invention, which comprises the hybridization of the immobilized nucleic acid with complementary and mismatch segments. Furthermore, the present invention relates to immobilized nucleic acids obtainable by the method of the invention, the use of accordingly immobilized nucleic acids for the production of nucleic acid arrays and a diagnostic kit, comprising an array of nucleic acids which are immobilized according to the present invention.

    摘要翻译： 本发明涉及用于将核酸固定在载体上的方法，其包括提供具有仅一种碱基类型的核苷酸序列的核酸和通过光交联将所述核酸固定在载体上，其中所述交联通过 光在约300-500nm的波长处进行，优选在365nm的波长下进行。 本发明还涉及用于分析根据本发明固定的核酸的方法，其包括将固定的核酸与互补和错配片段杂交。 此外，本发明涉及通过本发明的方法可获得的固定化核酸，使用相应地固定的核酸来生产核酸阵列和诊断试剂盒，其包含根据本发明固定的核酸阵列 发明。

    METHOD FOR TESTING AND QUALITY CONTROLLING OF NUCLEIC ACIDS ON A SUPPORT
    6.
    发明申请
    METHOD FOR TESTING AND QUALITY CONTROLLING OF NUCLEIC ACIDS ON A SUPPORT 审中-公开
    用于支持核酸的测试和质量控制的方法

    公开(公告)号：US20110207630A1

    公开(公告)日：2011-08-25

    申请号：US13121195

    申请日：2009-09-29

    申请人： Anke Pierik ,  Hendrik Roelof Stapert

    发明人： Anke Pierik ,  Hendrik Roelof Stapert

    IPC分类号： C40B30/04 ,  C40B40/06 ,  C07H21/04

    CPC分类号： C12Q1/6837 ,  C12Q2565/518 ,  C12Q2525/173

    摘要： The present invention relates to a method for testing nucleic acids on a support, comprising the immobilization of one or more nucleic acids via crosslinking, wherein each of the immobilized nucleic acids includes a stretch of nucleotides of only one basetype, the provision of labeled oligonucleotides complementary to said stretch of nucleotides, and the determination of a value indicative for the condition of said nucleic acids. The present invention further relates to a kit for testing nucleic acids on a support comprising an array of nucleic acids immobilized on a solid support, wherein each of the immobilized nucleic acids includes a stretch of nucleotides of only one basetype and a labeled oligonucleotide complementary to said stretch of nucleotides. The invention additionally concerns the use of a labeled oligonucleotide complementary to a stretch of nucleotides of only one basetype for testing the condition of nucleic acids immobilized on a solid support.

    摘要翻译： 本发明涉及一种用于在载体上测试核酸的方法，其包括通过交联固定一种或多种核酸，其中每个固定化的核酸包括仅一种碱基类型的一段核苷酸，提供标记的寡核苷酸互补 到所述核苷酸区段，以及确定指示所述核酸条件的值。 本发明还涉及一种用于在载体上测试核酸的试剂盒，其包含固定在固体支持物上的核酸阵列，其中每个固定化核酸包括仅一种碱基类型的核苷酸片段和与所述 核苷酸延伸。 本发明还涉及使用与仅一种碱基类型的一段核苷酸互补的标记的寡核苷酸来测试固定在固体支持物上的核酸的状态。

    Method for immobilizing nucleic acids on a support
    8.
    发明授权
    Method for immobilizing nucleic acids on a support 有权

    公开(公告)号：US09657338B2

    公开(公告)日：2017-05-23

    申请号：US13121206

    申请日：2009-09-22

    申请人： Anke Pierik ,  Hendrik Roelof Stapert

    发明人： Anke Pierik ,  Hendrik Roelof Stapert

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6837 ,  C12Q2525/173

    摘要： The present invention relates to a method for immobilizing nucleic acids on a support, comprising the provision of a nucleic acid with a stretch of nucleotides of only one basetype and the immobilization of said nucleic acid on a support by crosslinking by light, wherein said crosslinking by light is performed at a wavelength of about 300-500 nm, preferably at a wavelength of 365 nm. The present invention further relates to a method for the analysis of nucleic acids immobilized according to the invention, which comprises the hybridization of the immobilized nucleic acid with complementary and mismatch segments. Furthermore, the present invention relates to immobilized nucleic acids obtainable by the method of the invention, the use of accordingly immobilized nucleic acids for the production of nucleic acid arrays and a diagnostic kit, comprising an array of nucleic acids which are immobilized according to the present invention.

    Microelectronic sensor device for DNA detection
    9.
    发明授权
    Microelectronic sensor device for DNA detection 有权
    用于DNA检测的微电子传感器装置

    公开(公告)号：US08815162B2

    公开(公告)日：2014-08-26

    申请号：US12303448

    申请日：2007-05-24

    申请人： Erik Robbert Vossenaar ,  Marc Wilhelmus Gijsbert Ponjee ,  Hendrik Roelof Stapert ,  Mark Thomas Johnson

    发明人： Erik Robbert Vossenaar ,  Marc Wilhelmus Gijsbert Ponjee ,  Hendrik Roelof Stapert ,  Mark Thomas Johnson

    IPC分类号： G05D23/00

    CPC分类号： G01N21/6454 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1827 ,  C12Q1/6825 ,  G01N21/0332 ,  G01N21/6428 ,  G01N2021/6439 ,  C12Q2565/1015 ,  C12Q2527/101 ,  C12Q2527/107

    摘要： The invention relates to a microelectronic sensor device and a method for the investigation of biological target substances (20), for example oligonucleotides like DNA fragments. In one embodiment, the device comprises a reaction surface (RS) to which target specific reactants (10) are attached and which lies between a sample chamber (SC) and an array of selectively controllable heating elements (HE). The temperature profile in the sample chamber (SC) can be controlled as desired to provide for example conditions for a PCR and/or for a controlled melting of hybridizations. The reactant (10) and/or the target substance (20) comprises a label (12) with an observable property, like fluorescence, that changes if the target substance (20) is bound to the reactant (10), said property being detected by an array of sensor elements, for example photosensors (SE). The fluorescence of the label (12) may preferably be transferred by FRET to a different fluorescent label (22) or quenched if the target substance (20) is bound.

    摘要翻译： 本发明涉及一种微电子传感器装置和一种用于研究生物靶物质（20）的方法，例如DNA片段等寡核苷酸。 在一个实施方案中，所述装置包括反应表面（RS），目标特异性反应物（10）附着到所述反应表面（RS），并且位于样品室（SC）和选择性可控制的加热元件（HE）阵列之间。 样品室（SC）中的温度曲线可以根据需要进行控制，以提供例如PCR和/或受控熔化杂交的条件。 反应物（10）和/或目标物质（20）包含具有可观察性质（如荧光）的标记物（12），如果目标物质（20）与反应物（10）结合，则其变化，所述性质被检测 通过传感器元件阵列，例如光电传感器（SE）。 标记物（12）的荧光优选通过FRET转移到不同的荧光标记物（22），或者如果靶物质（20）被结合则被淬灭。

    Non-leaching adhesive system and its use in a liquid immersion objective
    10.
    发明授权
    Non-leaching adhesive system and its use in a liquid immersion objective 有权
    非浸出粘合剂系统及其在液体浸入物中的应用

    公开(公告)号：US07935213B2

    公开(公告)日：2011-05-03

    申请号：US12629155

    申请日：2009-12-02

    申请人： Emile Johannes Karel Verstegen ,  Johan George Kloosterboer ,  Hendrik Roelof Stapert ,  Jacobus Hermanus Maria Neijzen ,  Helmar van Santen

    发明人： Emile Johannes Karel Verstegen ,  Johan George Kloosterboer ,  Hendrik Roelof Stapert ,  Jacobus Hermanus Maria Neijzen ,  Helmar van Santen

    IPC分类号： B29C65/00 ,  B32B37/12 ,  B32B7/12

    CPC分类号： C09J135/02

    摘要： A non-leaching adhesive system and its use in a liquid immersion objective for immersion-writing of masters for optical discs are disclosed. The adhesive system comprises at least one monomer, selected from among the group of acrylate and methacrylate monomers, allylic monomers, norbornene monomers, hybrid monomers thereof, containing chemically different polymerizable groups, and multifunctional thiol monomers, provided that said thiol is used in combination with at least one of said non-thiol monomers; and a polymerization initiator. At least one of said monomers, not being a thiol, is provided with at least two functional polymerizable groups to obtain a crosslinked polymer network. The polymerization initiator is preferably an initiator that can be activated both thermally and with UV radiation. The adhesive system may further contain a reactive diluent. Further the use of the present adhesive system in mounting a liquid immersion objective is disclosed.

    摘要翻译： 公开了一种非浸出粘合剂系统及其在用于浸入式写入光盘的液浸物镜中的用途。 粘合剂体系包含至少一种选自丙烯酸酯和甲基丙烯酸酯单体的单体，烯丙基单体，降冰片烯单体，含有化学不同的可聚合基团的杂化单体和多官能硫醇单体，条件是所述硫醇与 所述非硫醇单体中的至少一种; 和聚合引发剂。 至少一种所述单体（不是硫醇）具有至少两个官能的可聚合基团以获得交联的聚合物网络。 聚合引发剂优选是能够以热辐射和UV辐射活化的引发剂。 粘合剂体系还可以含有反应性稀释剂。 此外，公开了本发明的粘合剂系统在安装液浸物镜中的用途。