公开(公告)号：US4994374A

公开(公告)日：1991-02-19

申请号：US362414

申请日：1989-05-01

申请人： Atsushi Nishikawa ,  Naoyuki Taniguchi ,  Isamu Takagahara

发明人： Atsushi Nishikawa ,  Naoyuki Taniguchi ,  Isamu Takagahara

IPC分类号： G01N33/50 ,  A61K47/48 ,  C12Q1/48 ,  G01N33/573 ,  G01N33/574

CPC分类号： A61K47/48238 ,  C12Q1/48 ,  G01N33/57438 ,  G01N33/57469

摘要： This invention relates to a method of diagnosing cancerous diseases, which comprises measuring the amount of UDP-N-acetylglucosamine:glycoprotein N-acetylglucosaminyl-transferase in body fluid and evaluating the increase in its amount for the diagnosis of hepatic diseases.AFP, CEA and .gamma.-glutamyltranspeptidase have hitherto been used as tumor markers for the diagnosis of hepatic cancer. But these conventional tumor markers show a positivity rate of about 60%, making early diagnosis almost impossible.The method of this invention employs UDP-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase as tumor marker, whereby early diagnosis of hepatic cancer can be made almost completely.

摘要翻译： PCT No.PCT / JP88 / 00898 Sec。 371日期：1989年5月1日 102（e）日期1989年5月1日PCT提交1988年9月6日PCT公布。 出版物WO89 / 02474 本发明涉及一种诊断癌性疾病的方法，其包括测量体液中UDP-N-乙酰葡糖胺：糖蛋白N-乙酰氨基葡糖转移酶的量，并评估其用于诊断的量的增加 肝病。 AFP，CEA和γ-谷氨酰转肽酶迄今已被用作肝癌诊断的肿瘤标志物。 但这些常规肿瘤标志物的阳性率约为60％，使得早期诊断几乎不可能。 本发明的方法采用UDP-N-乙酰氨基葡萄糖：糖蛋白N-乙酰葡糖胺基转移酶作为肿瘤标志物，从而能够几乎完全地进行肝癌的早期诊断。

公开(公告)号：US5834284A

公开(公告)日：1998-11-10

申请号：US910990

申请日：1997-08-14

申请人： Naoyuki Taniguchi ,  Atsushi Nishikawa ,  Nozomi Yamaguchi

发明人： Naoyuki Taniguchi ,  Atsushi Nishikawa ,  Nozomi Yamaguchi

IPC分类号： C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12R1/91 ,  C12N9/18

CPC分类号： C12N9/1051

摘要： A .beta.1,6-N-acetylglucosaminyl transferase having the following properties: (1) Action: it transfers N-acetylglucosamine from UDP-N-acetylglucosamine to .alpha.-6-D-mannoside; (2) Substrate specificity: it shows a reactivity of about 79% for GnGnF-bi-PA, about 125% for GnGnGn-tri-PA and about 66% for GnM-Pa, when taking a reactivity for GnGn-bi-PA as 100%; (3) Optimum pH: 6.2 to 6.3; (4) Inhibition, Activation and Stability: Mn.sup.2+ is not necessary for expression of activity, and the activity is not inhibited in the presence of 20 mM EDTA; (5) Molecular weight: about 73,000 as determined by SDS-PAGE in the absence of reducing agent; and about 73,000 and about 60,000 as determined in the presence of a reducing agent; (6) Km value: 133 .mu.M and 3.5 mM for acceptor GnGn-bi-PA and donor UDP-GlcNAc, respectively; and (7) It includes the following peptide fragments: (SEQ ID NO: 1) Thr-Pro-Trp-Gly-Lys (SEQ ID NO: 2) Asn-Ile-Pro-Ser-Tyr-Val (SEQ ID NO: 3) Val-Leu-Asp-Ser-Phe-Gly-Thr-Glu-Pro-Glu-Phe-Asn-His-Ala-Asn-Tyr-Ala (SEQ ID NO: 4) Asp-Leu-Gln-Phe-Leu-Leu (SEQ ID NO: 5) Asn-Thr-Asp-Phe-Phe-Ile-Gly, and gene coding for said enzyme, and a process for production of the enzyme.

摘要翻译： 具有以下性质的β1,6-己酰氨基葡糖转移酶：（1）作用：将N-乙酰葡糖胺从UDP-N-乙酰葡糖胺转移至α-6-D-甘露糖苷; （2）底物特异性：对GnGnF-bi-PA显示出约79％的反应性，GnGnGn-tri-PA为约125％，GnM-Pa为约66％ 100％; （3）最适pH：6.2〜6.3; （4）抑制，活化和稳定性：Mn2 +对于表达活性不是必需的，并且在20mM EDTA存在下不能抑制活性; （5）分子量：不含还原剂时通过SDS-PAGE测定的约73,000; 约73,000和约60,000，在还原剂存在下测定; （6）Km值分别为133μM和3.5mM，分别为受体GnGn-bi-PA和供体UDP-GlcNAc; （7）它包括以下肽片段：（SEQ ID NO：1）Thr-Pro-Trp-Gly-Lys（SEQ ID NO：2）Asn-Ile-Pro-Ser-Tyr-Val（SEQ ID NO： 3）Val-Leu-Asp-Ser-Phe-Gly-Thr-Glu-Pro-Glu-Phe-Asn-His-Ala-Asn-Tyr-Ala（SEQ ID NO：4）Asp-Leu-Gln-Phe- Leu-Leu（SEQ ID NO：5）Asn-Thr-Asp-Phe-Phe-Ile-Gly和编码所述酶的基因及其制备方法。

公开(公告)号：US5874271A

公开(公告)日：1999-02-23

申请号：US524828

申请日：1995-09-07

申请人： Atsushi Nishikawa ,  Yoshito Ihara ,  Masafumi Yoshimura ,  Shunichiro Taniguchi ,  Naoyuki Taniguchi

发明人： Atsushi Nishikawa ,  Yoshito Ihara ,  Masafumi Yoshimura ,  Shunichiro Taniguchi ,  Naoyuki Taniguchi

IPC分类号： A61P35/00 ,  C12N9/10 ,  C12N1/20

CPC分类号： C12N9/1051 ,  C12N9/1048

摘要： A gene encoding human glycosyltransferase (human GnT-III) and recombinant DNA method for producing the enzyme are provided. A cancerous metastasis inhibitor comprising GnT-III, or a gene thereof, and a method of inhibiting cancerous metastasis in a mammal are also provided.

公开(公告)号：US5876714A

公开(公告)日：1999-03-02

申请号：US975114

申请日：1997-11-20

申请人： Atsushi Nishikawa ,  Yoshito Ihara ,  Masafumi Yoshimura ,  Shunichiro Taniguchi ,  Naoyuki Taniguchi

发明人： Atsushi Nishikawa ,  Yoshito Ihara ,  Masafumi Yoshimura ,  Shunichiro Taniguchi ,  Naoyuki Taniguchi

IPC分类号： A61P35/00 ,  C12N9/10 ,  A61K38/45

CPC分类号： C12N9/1051 ,  C12N9/1048

摘要： A gene encoding human glycosyltransferase (human GnT-III) and recombinant DNA method for producing the enzyme are provided. A cancerous metastasis inhibitor comprising GnT-III, or a gene thereof, and a method of inhibiting cancerous metastasis in a mammal are also provided.

摘要翻译： 提供编码人糖基转移酶（人GnT-III）的基因和用于生产酶的重组DNA方法。 还提供了包含GnT-III或其基因的癌性转移抑制剂以及抑制哺乳动物中癌性转移的方法。

公开(公告)号：US5827687A

公开(公告)日：1998-10-27

申请号：US672569

申请日：1996-06-28

申请人： Nobuto Koyama ,  Eiji Miyoshi ,  Yoshito Ihara ,  Atsushi Nishikawa ,  Naoyuki Taniguchi

发明人： Nobuto Koyama ,  Eiji Miyoshi ,  Yoshito Ihara ,  Atsushi Nishikawa ,  Naoyuki Taniguchi

IPC分类号： C12N9/02 ,  C12N15/85 ,  C12P21/06

CPC分类号： C12N9/0069 ,  C12N15/85 ,  A01K2217/05

摘要： An isolated DNA having a promoter activity in an animal cell; a method for expressing a useful gene using the isolated DNA; and a method for producing a protein in an animal cell using the isolated DNA. The present invention provides a method for producing a desired gene product in a large quantity in an animal cell.

摘要翻译： 在动物细胞中具有启动子活性的分离的DNA; 使用分离的DNA表达有用基因的方法; 以及使用分离的DNA在动物细胞中产生蛋白质的方法。 本发明提供了在动物细胞中大量生产所需基因产物的方法。

公开(公告)号：US5707846A

公开(公告)日：1998-01-13

申请号：US405230

申请日：1995-03-16

申请人： Naoyuki Taniguchi ,  Atsushi Nishikawa ,  Nozomi Yamaguchi

发明人： Naoyuki Taniguchi ,  Atsushi Nishikawa ,  Nozomi Yamaguchi

IPC分类号： C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12R1/91

CPC分类号： C12N9/1051

摘要： A .beta.1,6-N-acetylglucosaminyl transferase having the following properties: (1) Action: it transfers N-acetylglucosamine from UDP-N-acetylglucosamine to .alpha.-6-D-mannoside; (2) Substrate specificity: it shows a reactivity of about 79% for GnGnF-bi-PA, about 125% for GnGnGn-tri-PA and about 66% for GnM-Pa, when taking a reactivity for GnGn-bi-PA as 100%; (3) Optimum pH: 6.2 to 6.3; (4) Inhibition, Activation and Stability: Mn.sup.2+ is not necessary for expression of activity, and the activity is not inhibited in the presence of 20 mM EDTA; (5) Molecular weight: about 73,000 as determined by SDS-PAGE in the absence of reducing agent; and about 73,000 and about 60,000 as determined in the presence of a reducing agent; (6) Km value: 133 .mu.M and 3.5 mM for acceptor GnGn-bi-PA and donor UDP-GlcNAc, respectively; and (7) It includes the following peptide fragments: (SEQ ID NO.1) Thr-Pro-Trp-Gly-Lys (SEQ ID NO.2) Asn-Ile-Pro-Ser-Tyr-Val (SEQ ID NO.3) Val-Leu-Asp-Ser-Phe-Gly-Thr-Glu-Pro-Glu-Phe-Asn-His-Ala-Asn-Tyr-Ala (SEQ ID NO.4) Asp-Leu-Gln-Phe-Leu-Leu (SEQ ID NO.5) Asn-Thr-Asp-Phe-Phe-Ile-Gly, and gene coding for said enzyme, and a process for production of the enzyme.

摘要翻译： 具有以下性质的β1,6-己酰氨基葡糖转移酶：（1）作用：将N-乙酰葡糖胺从UDP-N-乙酰葡糖胺转移至α-6-D-甘露糖苷; （2）底物特异性：对GnGnF-bi-PA显示出约79％的反应性，GnGnGn-tri-PA为约125％，GnM-Pa为约66％ 100％; （3）最适pH：6.2〜6.3; （4）抑制，活化和稳定性：Mn2 +对于表达活性不是必需的，并且在20mM EDTA存在下不能抑制活性; （5）分子量：不含还原剂时通过SDS-PAGE测定的约73,000; 约73,000和约60,000，在还原剂存在下测定; （6）Km值分别为133μM和3.5mM，分别为受体GnGn-bi-PA和供体UDP-GlcNAc; （7）它包括以下肽片段：（SEQ ID NO.1）Thr-Pro-Trp-Gly-Lys（SEQ ID NO.2）Asn-Ile-Pro-Ser-Tyr-Val（SEQ ID NO： 3）Val-Leu-Asp-Ser-Phe-Gly-Thr-Glu-Pro-Glu-Phe-Asn-His-Ala-Asn-Tyr-Ala（SEQ ID NO.4）Asp-Leu-Gln-Phe- Leu-Leu（SEQ ID NO.5）Asn-Thr-Asp-Phe-Phe-Ile-Gly，以及编码所述酶的基因及其制备方法。

公开(公告)号：US20140046114A1

公开(公告)日：2014-02-13

申请号：US14114042

申请日：2012-04-27

申请人： Atsushi Nishikawa ,  Youichi Saitoh ,  Asao Okada ,  Taishi Fukushima ,  Taiga Matsuzaki

发明人： Atsushi Nishikawa ,  Youichi Saitoh ,  Asao Okada ,  Taishi Fukushima ,  Taiga Matsuzaki

IPC分类号： A61N2/02

CPC分类号： A61N2/02 ,  A61B5/06 ,  A61N2/008 ,  A61N2/06

摘要： Provided is a therapeutic electromagnetic stimulation device for providing magnetic stimulation by applying a magnetic field to a specific site of an object person, the device including: a magnetic field generating means configured to generate one of a therapeutic magnetic field and a detection magnetic field for detection of information at least including positional information; magnetic field detecting means configured to detect intensities of components in a plurality of directions of one of the therapeutic magnetic field and the detection magnetic field at least at two detection positions; and a data generating means configured to generate custom data pairs for an individual object person, the custom data pairs being for deriving at least information of positions of the magnetic field generating means from the intensities of the corresponding components, the data generating means generating the custom data pairs using: (1) a sampling detection result of the detection of the intensities of the corresponding components in a state in which the magnetic field generating means are disposed respectively at a plurality of sampling spots near the specific site of the object person; and (2) a plurality of parent data pairs each including (a) at least information of a three-dimensional position of the magnetic field generating means pairing with (b) information of the intensities of the corresponding components of the magnetic field at a position having at least the information of the three-dimensional position, the information (a) and (b) being previously recorded.

摘要翻译： 本发明提供一种治疗电磁刺激装置，用于通过向对象人的特定部位施加磁场来提供磁刺激，所述装置包括：磁场产生装置，被配置为产生治疗磁场和用于检测的检测磁场之一 的信息至少包括位置信息; 磁场检测装置，被配置为至少在两个检测位置处检测治疗磁场和检测磁场之一的多个方向上的分量的强度; 以及数据生成装置，被配置为生成针对个人对象的自定义数据对，所述自定义数据对用于根据对应组件的强度导出至少磁场产生装置的位置的信息，所述数据生成装置生成定制 数据对，使用：（1）在将磁场产生装置分别设置在对象人的特定部位附近的多个采样点处的状态下检测相应部件的强度的采样检测结果; 和（2）多个母数据对，每个母数据对包括：（a）磁场产生装置的三维位置的信息至少与（b）在位置处的磁场的相应分量的强度的信息进行配对 至少具有三维位置的信息，先前记录信息（a）和（b）。

公开(公告)号：US20090267331A1

公开(公告)日：2009-10-29

申请号：US12086485

申请日：2006-12-13

申请人： Hideki Umeda ,  Atsushi Nishikawa ,  Teruji Kuroyanagi ,  Yasunari Sakai

发明人： Hideki Umeda ,  Atsushi Nishikawa ,  Teruji Kuroyanagi ,  Yasunari Sakai

IPC分类号： B60R21/16

CPC分类号： B60R21/232 ,  B60R2021/161 ,  B60R2021/23386

摘要： A vehicle occupant restraint apparatus is provided that can restrict the movement of the rear end side of an airbag to the outside of a vehicle. A folded body of the airbag 1 is installed along a roof side rail 3 and is inflatable by means of at least one inflator 4. Guide plates for guiding the extension of the airbag are provided on the roof side rail 3 above each pillar. An extended piece 1c of the airbag 1 is fastened to the rearmost guide plate 7, and thereby the movement of the inflated airbag 1 toward the outside of the vehicle is restricted.

摘要翻译： 提供一种车辆乘员约束装置，其可以将气囊的后端侧的运动限制到车辆的外部。 气囊1的折叠体沿屋顶侧轨道3安装，并且可通过至少一个充气机4充气。引导气囊延伸的引导板设置在每个支柱上方的车顶侧轨道3上。 安全气囊1的延伸片1c被紧固到最后面的引导板7，从而限制了膨胀的安全气囊1朝向车外的移动。

公开(公告)号：US20090187288A1

公开(公告)日：2009-07-23

申请号：US12308380

申请日：2007-06-15

申请人： Jun-ichi Shimada ,  Atsushi Nishikawa

发明人： Jun-ichi Shimada ,  Atsushi Nishikawa

IPC分类号： G05D1/12

CPC分类号： A61B34/70 ,  A61B34/30 ,  A61B90/30 ,  A61B2017/00212 ,  A61B2034/301 ,  A61B2090/061 ,  A61B2090/3614

摘要： A remote control system capable of precise operations or treatments in which the visual recognition and operation portions are as close to each other as possible, including: a) an imaging device for taking an image of the object; b) a manipulation tool having at its tip an illuminator for casting a spot light onto the object; c) a driver for changing the position of the manipulation tool; d) an external display unit for displaying an image; e) an input unit for allowing an operator to specify a position corresponding to the display of the external display unit; f) a distance calculator for calculating the distance between the position specified through the input unit and the position of the spot light cast from the illuminator onto the object; and g) a controller for controlling, based on the aforementioned distance, the driver so as to bring the tip of the manipulation tool closer to the specified position.

摘要翻译： 一种遥控系统，其能够精确地操作或处理，其中视觉识别和操作部分尽可能接近彼此，包括：a）用于拍摄对象的图像的成像装置; b）操作工具，在其顶端具有用于将点光投射到物体上的照明器; c）用于改变操作工具的位置的驱动器; d）用于显示图像的外部显示单元; e）用于允许操作者指定与外部显示单元的显示相对应的位置的输入单元; f）距离计算器，用于计算通过输入单元指定的位置与从照明器投射到物体上的点光的位置之间的距离; 以及g）用于基于前述距离控制驾驶员以使操纵工具的尖端更接近指定位置的控制器。

公开(公告)号：US20090041859A1

公开(公告)日：2009-02-12

申请号：US11887975

申请日：2006-04-07

申请人： Hitoshi Mizutani ,  Yoshiaki Miyamoto ,  Atsushi Nishikawa

发明人： Hitoshi Mizutani ,  Yoshiaki Miyamoto ,  Atsushi Nishikawa

IPC分类号： A61K33/18 ,  A61P17/02

CPC分类号： A61K47/06 ,  A61K9/0014 ,  A61K9/06 ,  A61K31/79 ,  A61K33/18

摘要： [Problems] The objective is to provide a new low-toxic drug for external use for intractable diseases, such as erosion, decubitus, and skin ulcer, that prevents cicatrization and reduces iodine accumulation on wound surfaces with sufficiently promoting healing.[Means for solving problem] Preparation of a wound treatment drug for external use containing oily bases and iodine but substantially no white soft sugar, which is a characteristic of the drug of this invention. The invented drug prevents infection through the wound surface due to a sufficient bactericidal effect of iodine, and promotes wound healing, prevents cicatrization and reduces iodine accumulation on the wound surface by maintenance of appropriate moisture in the wound surface due to a moisturizing effect of oily bases.

摘要翻译： [问题]目的是提供一种新的低毒药物，用于难治性疾病，如侵蚀，褥疮和皮肤溃疡，可以预防瘢痕形成，减少伤口表面的碘积累，充分促进愈合。 [解决问题的方法]作为本发明的药物的特征的含有油性基质和碘但基本上不含白色软糖的外用伤口治疗药物的制备。 本发明的药物由于碘的足够的杀菌作用而防止了伤口表面的感染，并且由于油性基质的保湿效果，通过维持伤口表面的适当水分，促进伤口愈合，防止伤口愈合并减少伤口表面的碘积聚 。