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公开(公告)号:US20210069698A1
公开(公告)日:2021-03-11
申请号:US16928225
申请日:2020-07-14
发明人: Xiao Guan , Mark P. White , Jason M. McEwen , Gang F. Wang , Kevin T. Chapman , Xiaohua Wang , Christine E. Sun
摘要: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
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公开(公告)号:US10407658B2
公开(公告)日:2019-09-10
申请号:US15282923
申请日:2016-09-30
发明人: Russell A. Newstrom , Andrew W. McFarland , Darcy K. Kelly-Greene , J. Tanner Nevill , Gang F. Wang
摘要: Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.
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公开(公告)号:US11492584B2
公开(公告)日:2022-11-08
申请号:US16537389
申请日:2019-08-09
发明人: Russell A. Newstrom , Andrew W. McFarland , Darcy K. Kelly-Greene , J. Tanner Nevill , Gang F. Wang
摘要: Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.
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公开(公告)号:US11434462B2
公开(公告)日:2022-09-06
申请号:US16427952
申请日:2019-05-31
发明人: Darcy K. Kelly-Greene , Russell A. Newstrom , Andrew W. McFarland , J. Tanner Nevill , Gang F. Wang
摘要: Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.
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公开(公告)号:US11365381B2
公开(公告)日:2022-06-21
申请号:US16920365
申请日:2020-07-02
发明人: Randall D. Lowe, Jr. , Kristin G. Beaumont , Aathavan Karunakaran , Natalie C. Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
摘要: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
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公开(公告)号:US11203018B2
公开(公告)日:2021-12-21
申请号:US16160816
申请日:2018-10-15
发明人: Troy A. Lionberger , Matthew E. Fowler , Phillip J. M. Elms , Kevin D. Loutherback , Randall D. Lowe, Jr. , Jian Gong , J. Tanner Nevill , Gang F. Wang , Gregory G. Lavieu , John A. Tenney , Aathavan Karunakaran , Anupam Singhal , I-Jong Lin
摘要: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.
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公开(公告)号:US10751715B1
公开(公告)日:2020-08-25
申请号:US15136481
申请日:2016-04-22
发明人: Xiao Guan , Mark P. White , Jason M. McEwen , Gang F. Wang , Kevin T. Chapman , Xiaohua Wang , Christine E. Sun
摘要: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
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公开(公告)号:US20200032193A1
公开(公告)日:2020-01-30
申请号:US16537389
申请日:2019-08-09
发明人: Russell A. NEWSTROM , Andrew W. McFarland , Darcy K. Kelly-Greene , J. Tanner Nevill , Gang F. Wang
摘要: Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.
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公开(公告)号:US20200017817A1
公开(公告)日:2020-01-16
申请号:US16427952
申请日:2019-05-31
发明人: Darcy K. KELLY-GREENE , Russell A. Newstrom , Andrew W. McFarland , J. Tanner Nevill , Gang F. Wang
摘要: Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.
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公开(公告)号:US10723988B2
公开(公告)日:2020-07-28
申请号:US15135707
申请日:2016-04-22
发明人: Randall D. Lowe, Jr. , Kristin Beaumont , Aathavan Karunakaran , Natalie Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
摘要: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
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