公开(公告)号：US20240027396A1

公开(公告)日：2024-01-25

申请号：US18324026

申请日：2023-05-25

申请人： BERKELEY LIGHTS, INC.

发明人： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC分类号： G01N27/447 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02

CPC分类号： G01N27/44791 ,  B01L3/502761 ,  B03C5/005 ,  B03C5/026 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C2201/26 ,  B01L2400/0454

摘要： Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

公开(公告)号：US11305283B2

公开(公告)日：2022-04-19

申请号：US16509166

申请日：2019-07-11

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC分类号： B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US11203018B2

公开(公告)日：2021-12-21

申请号：US16160816

申请日：2018-10-15

申请人： Berkeley Lights, Inc.

发明人： Troy A. Lionberger ,  Matthew E. Fowler ,  Phillip J. M. Elms ,  Kevin D. Loutherback ,  Randall D. Lowe, Jr. ,  Jian Gong ,  J. Tanner Nevill ,  Gang F. Wang ,  Gregory G. Lavieu ,  John A. Tenney ,  Aathavan Karunakaran ,  Anupam Singhal ,  I-Jong Lin

IPC分类号： B01L3/00 ,  G01N21/64 ,  B03C5/02 ,  B03C5/00 ,  B01L9/00 ,  G06T7/00 ,  G01N21/05

摘要： Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20200032193A1

公开(公告)日：2020-01-30

申请号：US16537389

申请日：2019-08-09

申请人： Berkeley Lights, Inc.

发明人： Russell A. NEWSTROM ,  Andrew W. McFarland ,  Darcy K. Kelly-Greene ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/12 ,  C12M1/32 ,  C12M3/00 ,  C12M1/02 ,  C12M1/34 ,  C12M1/36

摘要： Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.

公开(公告)号：US20200017817A1

公开(公告)日：2020-01-16

申请号：US16427952

申请日：2019-05-31

申请人： Berkeley Lights, Inc.

发明人： Darcy K. KELLY-GREENE ,  Russell A. Newstrom ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Gang F. Wang

IPC分类号： C12M1/00 ,  C12M1/32 ,  C12M1/12 ,  C12M1/02

摘要： Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.

公开(公告)号：US10376886B2

公开(公告)日：2019-08-13

申请号：US15843122

申请日：2017-12-15

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC分类号： B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US20180259482A1

公开(公告)日：2018-09-13

申请号：US15842124

申请日：2017-12-14

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC分类号： G01N27/447 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02

摘要： Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

公开(公告)号：US20180147576A1

公开(公告)日：2018-05-31

申请号：US15802174

申请日：2017-11-02

申请人： Berkeley Lights, Inc.

发明人： Gregory G. Lavieu ,  Annamaria Mocciaro ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Magali Soumillon ,  J. Tanner Nevill ,  Volker L.S. Kurz ,  Patricia A. Dyck ,  Ravi K. Ramenani

IPC分类号： B01L3/00 ,  C12M3/06 ,  C12N15/10 ,  G01N15/10

CPC分类号： B01L3/502761 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12M23/16 ,  C12N15/1003 ,  C12N15/1024 ,  C12N15/1086 ,  C12N2310/00 ,  C12N2510/00 ,  G01N15/10 ,  G01N2015/0288 ,  G01N2015/1081

摘要： Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

公开(公告)号：US09889445B2

公开(公告)日：2018-02-13

申请号：US14521447

申请日：2014-10-22

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M Jimena Loureiro

IPC分类号： B01L3/00 ,  G01N33/68 ,  G01N33/50 ,  G01N33/543 ,  G01N15/14 ,  B03C5/00 ,  B03C5/02

CPC分类号： B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0454 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/1484 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54313 ,  G01N33/6854

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US09744533B2

公开(公告)日：2017-08-29

申请号：US14965721

申请日：2015-12-10

申请人： Berkeley Lights, Inc.

发明人： Keith J. Breinlinger ,  Eric D. Hobbs ,  Daniele Malleo ,  J. Tanner Nevill ,  Mark P. White

IPC分类号： B01L3/00 ,  G01N1/40

CPC分类号： B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0454 ,  B01L2400/0457 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2001/4083

摘要： A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.