公开(公告)号：US10023906B2

公开(公告)日：2018-07-17

申请号：US15510904

申请日：2014-10-14

Applicant: BGI SHENZHEN CO., LIMITED

Inventor： Chunyu Geng ,  Rongrong Guo ,  Ruoying Chen ,  Lingyu He ,  Wenwei Zhang ,  Hui Jiang

IPC: C12Q1/68 ,  C12N9/22 ,  C12Q1/6853 ,  C12N15/10 ,  C40B40/08 ,  C12N11/06 ,  C12N9/00 ,  C12Q1/686 ,  C40B50/06 ,  C40B50/14 ,  C12Q1/6806 ,  C12N15/66

CPC classification number: C12Q1/6853 ,  B01J19/0046 ,  B01J2219/00529 ,  C12N9/22 ,  C12N9/93 ,  C12N11/06 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/1082 ,  C12N15/1093 ,  C12N15/66 ,  C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2565/537 ,  C40B40/08 ,  C40B50/06 ,  C40B50/14 ,  C40B60/14 ,  C12Q2521/525 ,  C12Q2535/122 ,  C12N15/1089 ,  C12Q2525/186 ,  C12Q2521/101

Abstract: Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagent kit thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5′ end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.

公开(公告)号：US20180044667A1

公开(公告)日：2018-02-15

申请号：US15510904

申请日：2014-10-14

Applicant: BGI SHENZHEN CO., LIMITED

Inventor： Chunyu Geng ,  Rongrong Guo ,  Ruoying Chen ,  Lingyu He ,  Wenwei Zhang ,  Hui Jiang

IPC: C12N15/10 ,  C12N11/06 ,  C12N9/00 ,  C40B50/14 ,  C12N15/66 ,  C12Q1/68 ,  C40B50/06 ,  C40B40/08 ,  C12N9/22

CPC classification number: C12Q1/6853 ,  B01J19/0046 ,  B01J2219/00529 ,  C12N9/22 ,  C12N9/93 ,  C12N11/06 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/1082 ,  C12N15/1093 ,  C12N15/66 ,  C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2565/537 ,  C40B40/06 ,  C40B40/08 ,  C40B50/06 ,  C40B50/14 ,  C40B60/14 ,  C12Q2521/525 ,  C12Q2535/122 ,  C12N15/1089 ,  C12Q2525/186 ,  C12Q2521/101

Abstract: Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagents thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5′ end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.

公开(公告)号：US20180291371A1

公开(公告)日：2018-10-11

申请号：US15510882

申请日：2014-11-26

Applicant: BGI SHENZHEN CO., LIMITED

Inventor： Chunyu Geng ,  Ruoying Chen ,  Yuan Jiang ,  Xia Zhao ,  Rongrong Guo ,  Lingyu He ,  Yaqiao Li ,  Wenwei Zhang ,  Hui Jiang ,  Radoje Drmanac

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1093 ,  C12N15/1089 ,  C12N15/66 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q2525/191 ,  C12Q2521/307 ,  C12Q2521/507 ,  C12Q2525/121 ,  C12Q2525/307 ,  C12Q2563/131

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.