公开(公告)号：US06605454B2

公开(公告)日：2003-08-12

申请号：US09816512

申请日：2001-03-22

申请人： Barbara Foley Barenburg ,  Jeremy Burdon ,  Yuk-Tong Chan ,  Xunhu Dai ,  Sean Gallagher ,  Piotr Grodzinski ,  Robert Marrero ,  Vijay Nair ,  David Rhine ,  Thomas Smekal

发明人： Barbara Foley Barenburg ,  Jeremy Burdon ,  Yuk-Tong Chan ,  Xunhu Dai ,  Sean Gallagher ,  Piotr Grodzinski ,  Robert Marrero ,  Vijay Nair ,  David Rhine ,  Thomas Smekal

IPC分类号： C12N1300

CPC分类号： H05B6/806 ,  C12N1/066 ,  C12N13/00

摘要： A microwave device has a monolithic microwave integrated circuit (MMIC) disposed therein for applying microwave radiation to a microfluidic structure, such as a chamber, defined in the device. The microwave radiation from the MMIC is useful for heating samples introduced into the microfluidic structure and for effecting lysis of cells in the samples. Microfabrication techniques allow the fabrication of MMICs that perform heating and cell lysing of samples having volumes in the microliter to picoliter range.

公开(公告)号：US06572830B1

公开(公告)日：2003-06-03

申请号：US09337086

申请日：1999-06-21

申请人： Jeremy W. Burdon ,  Rong-Fong Huang ,  David Wilcox ,  Nicholas J. Naclerio ,  Cynthia Ann Gorsuch Briscoe ,  Piotr Grodzinski ,  Huinan Yu ,  Robert Marrero ,  Sean Ross Gallagher ,  Yuk-Tong Chan ,  Barbara McNeil Foley ,  Xunhu Dai

发明人： Jeremy W. Burdon ,  Rong-Fong Huang ,  David Wilcox ,  Nicholas J. Naclerio ,  Cynthia Ann Gorsuch Briscoe ,  Piotr Grodzinski ,  Huinan Yu ,  Robert Marrero ,  Sean Ross Gallagher ,  Yuk-Tong Chan ,  Barbara McNeil Foley ,  Xunhu Dai

IPC分类号： B01J1000

CPC分类号： G01N30/6069 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00495 ,  B01J2219/00783 ,  B01J2219/00835 ,  B01J2219/00873 ,  B01J2219/00966 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0874 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0439 ,  B32B18/00 ,  B32B2038/042 ,  B32B2311/06 ,  B32B2311/08 ,  B32B2315/02 ,  B81B2201/058 ,  B81C1/00119 ,  B81C2201/019 ,  C04B2235/6562 ,  C04B2237/341 ,  C04B2237/343 ,  C04B2237/348 ,  C04B2237/50 ,  C04B2237/62 ,  C04B2237/68 ,  C04B2237/704 ,  C40B60/14 ,  F04B19/006 ,  F28D9/00 ,  F28D2021/0029 ,  F28F13/00 ,  F28F2260/02 ,  G01N30/60 ,  G01N30/6004 ,  G01N30/6095 ,  G01N2030/025 ,  G01N2030/528 ,  G01N2035/00158 ,  G01N2035/00237 ,  H05K1/0272 ,  H05K1/0306 ,  H05K3/4611 ,  H05K3/4629

摘要： A multilayered microfluidic device having a substantially monolithic structure is formed by sintering together a plurality of green-sheet layers. The substantially monolithic structure has an inlet port for receiving fluid, an outlet port for releasing fluid, and an interconnection between the inlet port and the outlet port. The substantially monolithic structure may also include a variety of components to enable useful interaction with the fluid, such as electrically conductive pathways, heaters, fluid sensors, fluid motion transducers, and optically transmissive portions. The components are preferably fabricated using thick-film or green-sheet technology and are preferably co-fired with and sintered to the green-sheet layers to become integral with the substantially monolithic structure.

摘要翻译： 具有基本上整体结构的多层微流体装置通过将多个绿色层层烧结在一起而形成。 基本上单片结构具有用于接收流体的入口端口，用于释放流体的出口以及入口和出口之间的互连。 基本上单片结构还可以包括各种部件以使得能够与诸如导电通路，加热器，流体传感器，流体运动换能器和光学透射部分之类的流体有用的相互作用。 这些组分优选使用厚膜或生片技术制造，并且优选与生片层共烧并烧结成与基本上整体的结构一体。

公开(公告)号：US06984516B2

公开(公告)日：2006-01-10

申请号：US10340057

申请日：2003-01-10

申请人： Cynthia G. Briscoe ,  Huinan Yu ,  Piotr Grodzinski ,  Robert Marrero ,  Jeremy W. Burdon ,  Rong-Fong Huang

发明人： Cynthia G. Briscoe ,  Huinan Yu ,  Piotr Grodzinski ,  Robert Marrero ,  Jeremy W. Burdon ,  Rong-Fong Huang

IPC分类号： C12M1/34 ,  C12P19/34 ,  B01J19/08

CPC分类号： G01N30/6069 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00495 ,  B01J2219/00783 ,  B01J2219/00835 ,  B01J2219/00873 ,  B01J2219/00966 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0874 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0439 ,  B32B18/00 ,  B32B2038/042 ,  B32B2311/06 ,  B32B2311/08 ,  B32B2315/02 ,  B81B2201/058 ,  B81C1/00119 ,  B81C2201/019 ,  C04B2235/6562 ,  C04B2237/341 ,  C04B2237/343 ,  C04B2237/348 ,  C04B2237/50 ,  C04B2237/62 ,  C04B2237/68 ,  C04B2237/704 ,  C40B60/14 ,  F04B19/006 ,  F28D9/00 ,  F28D2021/0029 ,  F28F13/00 ,  F28F2260/02 ,  G01N30/60 ,  G01N30/6004 ,  G01N30/6095 ,  G01N2030/025 ,  G01N2030/528 ,  G01N2035/00158 ,  G01N2035/00237 ,  H05K1/0272 ,  H05K1/0306 ,  H05K3/4611 ,  H05K3/4629

摘要： A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.

公开(公告)号：US06544734B1

公开(公告)日：2003-04-08

申请号：US09460281

申请日：1999-12-09

申请人： Cynthia G. Briscoe ,  Huinan Yu ,  Piotr Grodzinski ,  Robert Marrero ,  Jeremy W. Burdon ,  Rong-Fong Huang

发明人： Cynthia G. Briscoe ,  Huinan Yu ,  Piotr Grodzinski ,  Robert Marrero ,  Jeremy W. Burdon ,  Rong-Fong Huang

IPC分类号： C12Q168

CPC分类号： G01N30/6069 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00495 ,  B01J2219/00783 ,  B01J2219/00835 ,  B01J2219/00873 ,  B01J2219/00966 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0874 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0439 ,  B32B18/00 ,  B32B2038/042 ,  B32B2311/06 ,  B32B2311/08 ,  B32B2315/02 ,  B81B2201/058 ,  B81C1/00119 ,  B81C2201/019 ,  C04B2235/6562 ,  C04B2237/341 ,  C04B2237/343 ,  C04B2237/348 ,  C04B2237/50 ,  C04B2237/62 ,  C04B2237/68 ,  C04B2237/704 ,  C40B60/14 ,  F04B19/006 ,  F28D9/00 ,  F28D2021/0029 ,  F28F13/00 ,  F28F2260/02 ,  G01N30/60 ,  G01N30/6004 ,  G01N30/6095 ,  G01N2030/025 ,  G01N2030/528 ,  G01N2035/00158 ,  G01N2035/00237 ,  H05K1/0272 ,  H05K1/0306 ,  H05K3/4611 ,  H05K3/4629

摘要： A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.

摘要翻译： 多层微流体DNA分析系统包括细胞裂解室，DNA分离室，DNA扩增室和DNA检测系统。 多层微流体DNA分析系统被提供为由多个烧结在一起的生片层组成的基本上整体的结构。 基本上整体的结构在其中限定了用于加热DNA扩增室的装置和用于冷却DNA扩增室的装置。 用于加热的装置和用于冷却的装置用于循环DNA扩增室的温度，以进行DNA扩增过程如PCR所需。