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    Pretreatment of Ligno-Cellulosic Biomass with Sulfonation
    1.
    发明申请
    Pretreatment of Ligno-Cellulosic Biomass with Sulfonation 审中-公开
    用磺化预处理木质纤维素生物质

    公开(公告)号：US20110124056A1

    公开(公告)日：2011-05-26

    申请号：US12894042

    申请日：2010-09-29

    申请人： Benjamin Levie ,  Rajesh Gupta ,  Johnway Gao ,  Dwight Anderson

    发明人： Benjamin Levie ,  Rajesh Gupta ,  Johnway Gao ,  Dwight Anderson

    IPC分类号： C12P19/04 ,  C12P19/02 ,  C13K1/02

    CPC分类号： C13K1/02 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  D21C3/06 ,  D21C11/0007

    摘要： Provided are methods for the pretreatment of ligno-cellulosic biomass such as softwoods with bisulfite such as ammonium bisulfite without the need for exogenous acid. In one variation, a method of pretreating ligno-cellulosic biomass is provided including the following steps: a) providing ligno-cellulosic biomass; b) contacting the ligno-cellulosic biomass with a solution comprising bisulfite at an amount between 1 and 10% of a dry weight of the ligno-cellulosic biomass to form a slurry; c) heating the slurry to a first temperature of 150-210° C. for a first period of time to form a first mixture; d) cooling the first mixture to a second temperature of 100-200° C. to form a second mixture; and e) maintaining the second mixture at the second temperature for a second period of time to form pretreated ligno-cellulosic biomass; wherein the first temperature is higher than the second temperature.

    摘要翻译： 提供了用于预处理木质纤维素生物质的方法，例如具有亚硫酸氢盐的软木，例如亚硫酸氢铵，而不需要外源性酸。 在一个实施方案中，提供了一种预处理木质纤维素生物质的方法，包括以下步骤：a）提供木质纤维素生物质; b）使木质纤维素生物质与包含亚硫酸氢盐的溶液接触，其量为木质纤维素生物质的干重的1至10％，以形成浆料; c）将所述浆料加热至第一温度为150-210℃，持续第一时间以形成第一混合物; d）将第一混合物冷却至100-200℃的第二温度以形成第二混合物; 和e）将所述第二混合物保持在所述第二温度第二时间段以形成预处理的木质纤维素生物质; 其中所述第一温度高于所述第二温度。

    SOLID LIGNOCELLULOSIC HYDROLYSATE AND METHODS TO PREPARE A SOLID LIGNOCELLULOSIC HYDROLYSATE
    2.
    发明申请
    SOLID LIGNOCELLULOSIC HYDROLYSATE AND METHODS TO PREPARE A SOLID LIGNOCELLULOSIC HYDROLYSATE 审中-公开
    固体水杨酸水解产物及其制备方法制备固体水杨酸

    公开(公告)号：US20130118483A1

    公开(公告)日：2013-05-16

    申请号：US13294867

    申请日：2011-11-11

    申请人： Johnway Gao ,  Dwight Anderson ,  Benjamin Levie

    发明人： Johnway Gao ,  Dwight Anderson ,  Benjamin Levie

    IPC分类号： C13B50/00 ,  C13K1/02

    CPC分类号： C13K1/04 ,  C12P7/10 ,  C12P19/14 ,  C13K13/007 ,  D21C1/04 ,  D21C3/00 ,  D21C5/005 ,  Y02E50/16 ,  Y02E50/343

    摘要： The present disclosure provides a solid lignocellulosic hydrolysate and methods to prepare the solid lignocellulosic hydrolysate from a woody biomass or an herbaceous biomass. The solid lignocellulosic hydrolysate may be used in the production of biofuels, bioproducts, and food products. The solid lignocellulosic hydrolysate allows for ease of storage, ease of transportation and handling of the solid lignocellulosic hydrolysate, and ease of use in biological or fermentation processes or chemical processes for the production of biofuel, bioproducts, chemicals and food products due to the bulk handling characteristics (e.g., solubility and rate of dissolution) of the solid lignocellulosic hydrolysate.

    摘要翻译： 本公开提供了一种固体木质纤维素水解物和从木质生物质或草本生物质制备固体木质纤维素水解产物的方法。 固体木质纤维素水解产物可用于生产生物燃料，生物产品和食品。 固体木质纤维素水解产物容易储存，易于运输和处理固体木质纤维素水解产物，并且易于在生物或发酵过程中使用或由于大批量处理而用于生产生物燃料，生物产品，化学品和食品的化学方法 固体木质纤维素水解产物的特征（例如溶解度和溶解速率）。

    METHODS OF ENABLING ENZYMATIC HYDROLYSIS AND FERMENTATION OF LIGNOCELLULOSIC BIOMASS WITH PRETREATED FEEDSTOCK FOLLOWING HIGH SOLIDS STORAGE IN THE PRESENCE OF ENZYMES
    4.
    发明申请
    METHODS OF ENABLING ENZYMATIC HYDROLYSIS AND FERMENTATION OF LIGNOCELLULOSIC BIOMASS WITH PRETREATED FEEDSTOCK FOLLOWING HIGH SOLIDS STORAGE IN THE PRESENCE OF ENZYMES 审中-公开
    在酶的存在下，利用预处理饲料进行酶促水解和发酵的低密度生物质的方法

    公开(公告)号：US20120264178A1

    公开(公告)日：2012-10-18

    申请号：US13416911

    申请日：2012-03-09

    申请人： Dwight ANDERSON ,  Johnway Gao ,  Benjamin Levie

    发明人： Dwight ANDERSON ,  Johnway Gao ,  Benjamin Levie

    IPC分类号： C12P19/02 ,  C12P19/00

    CPC分类号： C12P7/10 ,  C12P19/00 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/16

    摘要： The present invention provides methods of producing pretreated lignocellulosic biomass combined with enzymes for the storage and transporation of the pretreated lignocellulosic biomass that may be used in biofuel and bioproduct production. The methods allows the coexistence of the pretreated lignocellulosic biomass and the enzymes during storage and transporation, the immediate hydrolysis of the pretreated lignocellulosic biomass to produce sugars, without further addition of enzymes, in a biofuel or bioproduct production site, the enhancement of the final hydrolytic activity of the pretreated lignocellulosic biomass, and/or the reduction in sensitivity of the inhibitors in the pretreated lignocellulosic biomass.

    摘要翻译： 本发明提供了生产预处理的木质纤维素生物质与酶结合的方法，用于储存和运输可用于生物燃料和生物产物生产的预处理的木质纤维素生物质。 这些方法允许在储存和运输过程中预处理的木质纤维素生物质和酶的共存，预处理的木质纤维素生物质在生物燃料或生物产物生产位点中立即水解而不进一步加入酶以产生糖，增强最终的水解 预处理的木质纤维素生物质的活性和/或抑制剂在预处理的木质纤维素生物质中的敏感性的降低。

    SAMPLE PREPARATION FOR SPECTROSCOPY ANALYSIS
    5.
    发明申请
    SAMPLE PREPARATION FOR SPECTROSCOPY ANALYSIS 审中-公开
    光谱分析样品制备

    公开(公告)号：US20120320365A1

    公开(公告)日：2012-12-20

    申请号：US13597418

    申请日：2012-08-29

    申请人： Andrew P. Bartko ,  Johnway Gao

    发明人： Andrew P. Bartko ,  Johnway Gao

    IPC分类号： G01N1/28 ,  G01J3/44

    CPC分类号： G01N1/30 ,  G01N1/312 ,  G01N35/00029 ,  Y10T436/2575

    摘要： A sample is cleaned up for spectroscopic analysis by receiving a slide substrate having the sample thereon, fixing the sample to a substrate surface of the slide substrate by applying heat to the slide substrate for a predetermined heating time and incubating the sample on the slide substrate for a predetermined incubation time after fixing the sample to the slide substrate. The sample is further cleaned by washing the sample on the slide substrate after the sample has been incubated and drying the sample by applying heat to the slide substrate for a predetermined drying time, wherein the sample on the slide substrate after drying has retained particles of interest and interferant particles are removed from the substrate. A substrate is also provided for sample collection, which is culturable and Raman silent.

    摘要翻译： 通过接收具有样品的载玻片基板将样品清理干净，用于将样品固定在载玻片基板的基板表面上，通过向滑动基板施加预定加热时间并将样品温育在载玻片基板上 在将样品固定到载玻片基板之后的预定孵育时间。 通过在将样品温育并将样品通过向滑动基底施加热量干燥样品进行预定的干燥时间之后，通过在载玻片基板上洗涤样品来进一步清洗样品，其中在干燥后的载玻片基板上的样品已经保留了感兴趣的颗粒 并且从衬底去除干燥剂颗粒。 还提供了用于样品收集的底物，其是可培养的和拉曼沉默的。

    Production of human coagulation factor VIII from plant cells and whole plants
    6.
    发明申请
    Production of human coagulation factor VIII from plant cells and whole plants 审中-公开
    从植物细胞和整株植物生产人凝血因子VIII

    公开(公告)号：US20050060775A1

    公开(公告)日：2005-03-17

    申请号：US10863969

    申请日：2004-06-08

    申请人： Brian Hooker ,  Daniel Anderson ,  Johnway Gao ,  Ziyu Dai

    发明人： Brian Hooker ,  Daniel Anderson ,  Johnway Gao ,  Ziyu Dai

    IPC分类号： A01H1/00 ,  C12N15/82

    CPC分类号： C12N15/8257

    摘要： The invention includes methods for production of a polypeptide having factor VIII activity by introduction of a polynucleotide construct into a plant cell. The construct includes an encoding sequence for a polypeptide of coagulation factor VIII or a functional variant thereof. The plant cell is cultured or regenerated into a plant and the polypeptide or functional variant of factor VIII is expressed therein. The invention also includes vectors, plant cells, plant tissues, plants and seeds containing a polynucleotide sequence encoding a functional variant of human coagulation factor VIII. The invention further includes a recombinant DNA molecule having a promoter which is functional in plants operably linked to a coding sequence which codes for a polynucleotide having coagulation factor VIII activity.

    摘要翻译： 本发明包括通过将多核苷酸构建体引入植物细胞来生产具有因子VIII活性的多肽的方法。 该构建体包含凝血因子VIII多肽或其功能变体的编码序列。 将植物细胞培养或再生成植物，并在其中表达因子VIII的多肽或功能变体。 本发明还包括含有编码人凝血因子VIII功能变体的多核苷酸序列的载体，植物细胞，植物组织，植物和种子。 本发明还包括具有启动子的重组DNA分子，该启动子在与可编码具有凝血因子VIII活性的多核苷酸的编码序列可操作地连接的植物中是功能性的。

    Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species
    8.
    发明授权
    Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species 失效
    产生乳酸的真菌根霉的3-磷酸甘油酸激酶基因2的启动子序列和在真菌种中表达感兴趣的基因的方法

    公开(公告)号：US06528636B1

    公开(公告)日：2003-03-04

    申请号：US09535851

    申请日：2000-03-27

    申请人： Johnway Gao ,  Rodney S. Skeen

    发明人： Johnway Gao ,  Rodney S. Skeen

    IPC分类号： C07H2104

    CPC分类号： C12N9/1217 ,  C12N15/80

    摘要： The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 2 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.

    摘要翻译： 本发明提供了产生乳酸的丝状真菌菌株，米根霉（Rhizopus oryzae）的磷酸甘油酸激酶基因2的启动子克隆发现。 分离的启动子可以在各种碳水化合物条件下组成型调节基因表达。 此外，本发明还提供了用于在米根霉（Rhizopus oryzae）中外源基因转化的整合载体的设计。

    Sample preparation for spectroscopy analysis
    9.
    发明授权
    Sample preparation for spectroscopy analysis 有权
    光谱分析样品制备

    公开(公告)号：US09034278B2

    公开(公告)日：2015-05-19

    申请号：US13597418

    申请日：2012-08-29

    申请人： Andrew P. Bartko ,  Johnway Gao

    发明人： Andrew P. Bartko ,  Johnway Gao

    IPC分类号： A61B10/00 ,  G01N35/00 ,  G01N1/31

    CPC分类号： G01N1/30 ,  G01N1/312 ,  G01N35/00029 ,  Y10T436/2575

    摘要： A sample is cleaned up for spectroscopic analysis by receiving a slide substrate having the sample thereon, fixing the sample to a substrate surface of the slide substrate by applying heat to the slide substrate for a predetermined heating time and incubating the sample on the slide substrate for a predetermined incubation time after fixing the sample to the slide substrate. The sample is further cleaned by washing the sample on the slide substrate after the sample has been incubated and drying the sample by applying heat to the slide substrate for a predetermined drying time, wherein the sample on the slide substrate after drying has retained particles of interest and interferant particles are removed from the substrate. A substrate is also provided for sample collection, which is culturable and Raman silent.

    摘要翻译： 通过接收具有样品的载玻片基板将样品清理干净，用于将样品固定在载玻片基板的基板表面上，通过向滑动基板施加预定加热时间并将样品温育在载玻片基板上 在将样品固定到载玻片基板之后的预定孵育时间。 通过在将样品温育并将样品通过向滑动基底施加热量预定的干燥时间来干燥样品之后，通过在载玻片基板上洗涤样品来进一步清洗样品，其中在干燥后的载玻片基板上的样品已经保留了感兴趣的颗粒 并且从衬底去除干燥剂颗粒。 还提供了用于样品收集的底物，其是可培养的和拉曼沉默的。

    Biological and chemical monitoring
    10.
    发明授权
    Biological and chemical monitoring 有权
    生物和化学监测

    公开(公告)号：US07993585B2

    公开(公告)日：2011-08-09

    申请号：US11486939

    申请日：2006-07-14

    申请人： Rodney S. Black ,  Jeffrey P. Carpenter ,  Johnway Gao ,  Jerome U. Gilberry ,  John J. Kester ,  Clark A. Morrow

    发明人： Rodney S. Black ,  Jeffrey P. Carpenter ,  Johnway Gao ,  Jerome U. Gilberry ,  John J. Kester ,  Clark A. Morrow

    IPC分类号： G01N31/00 ,  G06F19/00

    CPC分类号： G01N15/1463 ,  G01N2015/0261 ,  G01N2015/1488 ,  Y10T436/11 ,  Y10T436/115831

    摘要： A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.

    摘要翻译： 提供了一种生物和化学检测系统，用于检测和识别感兴趣的生物和/或化学颗粒物。 生物和化学检测系统包括收集器，第一光学装置，第二光学装置和处理器。 收集器被配置为将从流体流中吸取的微粒沉积到样品基底上以限定样品区域。 第一光学装置相对于样本区域的至少一部分导出第一数据，其被分析以确定至少一个视场和/或特定目标位置。 然后，第二光学装置在每个确定的目标位置询问样品区域，例如使用拉曼光谱法，产生询问数据。 处理器基于询问数据的分析来确定样本区域是否包括预期的生物或化学颗粒物，并且如果识别出预定的感兴趣的生物或化学颗粒物，则触发诸如报警或消息的事件。