摘要:
Flow cell devices and methods for using the devices are disclosed. In one aspect, the flow cell devices are used to expose substrate surfaces comprising biopolymers or monomers to a desired fluid. Workstations are also provided including a flow cell device and one or more station(s) for processing a substrate, such as one or more of a printer, reaction chamber, wash chamber, and scanner. Computer program products for implementing functions of the devices and workstations and for performing the methods are also disclosed.
摘要:
Methods and apparatus are disclosed for preparing an array of polymeric compounds on a substrate. Drops of polymer subunits are dispensed to a surface of a substrate from two or more drop dispensing modules wherein each module comprises dispensers for dispensing a respective polymer-forming reagent. At least two of the modules and a substrate are brought into drop dispensing relationship in at least one step prior to conducting the step of preparing the surface for repeating the drop-dispensing step. Next, the surface is subjected to reagents to prepare the surface for repeating the drop-dispensing step. The above steps are repeated for a sufficient number of cycles to prepare the array of polymeric compounds.
摘要:
The subject invention provides methods and devices for fabricating chemical arrays. Also provided are chemical arrays fabricated according to the subject methods, and methods of using chemical arrays produced according to the subject invention.
摘要:
Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group generation step. A feature of the subject methods is that the functional group generation step is performed by sequentially flowing a plurality of different fluids across the surface of a substrate in a manner such that the surface is not contacted with a with a triple phase interface line. Also provided are the arrays produced using the subject methods, as well as methods for use of the arrays and kits that include the same.
摘要:
A method, a system, and a computer-readable medium storing a program for determining array quality during in-situ manufacturing of an array includes determining an average feature coverage fraction of the array, determining a background area fraction of the array, measuring a contact angle of the background, and determining the array quality based upon the measured, feature contact angle.
摘要:
Methods are disclosed for selecting a combination of nucleic acid sample pairs for evaluating the ability of an oligonucleotide probe to measure differential expression of genes. Differential gene expression experiments are conducted using (i) nucleic acid sample pairs and (ii) nucleic acid probes immobilized on a substrate, the probes representing a set of genes. The number of genes in the set is a portion of an expected number of genes in a sample. A nucleic acid sample pair combination is selected based on the members of the combination having a maximized number of genes from the set of genes that exhibit differential expression and a minimized number of the genes that do not exhibit differential expression.
摘要:
Methods for fabricating chemical arrays are provided. Aspects of the invention include methods for providing solid activator in feature locations of a substrate surface. Aspects of the invention also include substrates having solid activator present in feature locations thereof, arrays produced from the substrates, methods for use of the arrays and kits that include the arrays.
摘要:
Methods of producing nucleic acid molecules using an in situ nucleic acid synthesis protocol are provided. The method can comprise contacting a substrate comprising an attached blocked nucleoside monomer or polymer with a deblocking fluid to remove the blocking group, thereby generating an unblocked attached nucleoside monomer or polymer; displacing the deblocking fluid from the substrate surface comprising the attached unblocked nucleoside monomer or polymer with a purging fluid; and reacting the attached unblocked nucleoside monomer or polymer with another blocked nucleoside monomer. Nucleic acid molecules produced by the methods are also provided and can be attached to or released from the substrate (e.g., provided in solution or in a lyophilized form).
摘要:
Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group generation step, the latter of which may include: (a) oxidation and (b) deblocking substeps, and optionally a capping substep. A feature of the subject methods is that, following deblock of the surface, the deblocking fluid is displaced or purged from the surface using a fluid of different density, e.g., an oxidization fluid or wash fluid. Also provided are the arrays produced using the subject methods, as well as methods for use of the arrays and kits that include the same.
摘要:
Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group generation step, the latter of which may include: (a) oxidation and (b) deblocking substeps, and optionally a capping substep. A feature of the subject methods is that, following deblock of the surface, the deblocking fluid is displaced from the surface using a lower density wash fluid. Also provided are the arrays produced using the subject methods, as well as methods for use of the arrays and kits that include the same.