Alkyl ether sulfates
    5.
    发明授权
    Alkyl ether sulfates 有权
    烷基醚硫酸盐

    公开(公告)号:US07863479B2

    公开(公告)日:2011-01-04

    申请号:US10588217

    申请日:2005-02-10

    摘要: The present invention relates to alkyl ether sulfate salts of the general formula I RO—(CH2CH2O)x—(CH2—CHR1O)y—(CH2CH2O)zSO3−M+  (I), where R is an unbranched or branched C8-C18-alkyl radical or mixtures of different unbranched or branched C8-C18-alkyl radicals, R1 is an aliphatic radical selected from the group consisting of methyl and ethyl, M+ is a cation, selected from the group consisting of alkali metals, NH4+ and HNR23+, where R2 is selected from the group consisting of unbranched or branched alkyl radicals, CH2CH2OH and CH2CH(OH)CH3, x has a mean value of 0-3, y has a mean value of 1-10, z has a mean value of 0-30, and the quotient A = c ⁢ ⁢ m ⁢ ⁢ c ⁡ ( RO - ( CH 2 ⁢ CH 2 ⁢ O ) z ⁢ SO 3 - ⁢ M + ) c ⁢ ⁢ m ⁢ ⁢ c ( RO - ( CH 2 ⁢ CH 2 ⁢ O ) x - ( CH 2 - CHR 1 ⁢ O ) y - ( CH 2 ⁢ CH 2 ⁢ O ) z ⁢ SO 3 - ⁢ M + ) ⁢ ⁢ is > 1 and to their use as anion surfactant component in laundry detergents and cleaning compositions, in chemical engineering applications or in cosmetics formulations.

    摘要翻译: 本发明涉及通式为RO-(CH2CH2O)x-(CH2-CHR1O)y-(CH2CH2O)zSO3-M +(Ⅰ)的烷基醚硫酸盐,其中R是无支链或支链C 8 -C 18 - 烷基 基团或不同的未支化或支链C 8 -C 18烷基的混合物,R 1是选自甲基和乙基的脂族基,M +是选自碱金属,NH 4 +和HNR 23 +的阳离子,其中R 2 选自无支链或支链烷基,CH 2 CH 2 OH和CH 2 CH(OH)CH 3,x的平均值为0-3,y的平均值为1-10,z的平均值为0-30 (RO - (CH 2 CH 2 O)z SO 3 - M +)c⁢m⁢c(RO - (CH 2 CH 2 O)x - (CH 2 - CHR 1 O)y - (CH 2 CH 2 O)z SO 3 - M +)¶> 1,并且它们用作衣物中的阴离子表面活性剂组分 洗涤剂和清洁剂,在化学工程中 使用方法或化妆品配方。

    Modified nitrilases and their use in methods for the production of carboxylic acids
    8.
    发明授权
    Modified nitrilases and their use in methods for the production of carboxylic acids 有权
    改性腈水解酶及其在羧酸生产方法中的应用

    公开(公告)号:US07985572B2

    公开(公告)日:2011-07-26

    申请号:US10546611

    申请日:2004-02-24

    摘要: The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

    摘要翻译: 本发明涉及具有改进的基板接受性的新腈水解酶,获得它们的方法和使用上述腈水解酶。 所述腈水解酶由多肽序列编码,其在与野生型产碱杆菌粪便中的位置296相对应的位置包含不是酪氨酸的氨基酸。 本发明还涉及编码所述腈水解酶的核酸序列和氨基酸序列,包含所述核酸序列,包含核酸序列或exPression构建体的载体的表达构建体,包含核酸序列,表达构建体或生物体 向量 本发明还涉及从外消旋腈制备羧酸,优选取代的手性羧酸的方法。

    Modified nitrilases and their use in methods for the production of carboxylic acids
    9.
    发明申请
    Modified nitrilases and their use in methods for the production of carboxylic acids 有权
    改性腈水解酶及其在羧酸生产方法中的应用

    公开(公告)号:US20060259999A1

    公开(公告)日:2006-11-16

    申请号:US10546611

    申请日:2004-02-24

    IPC分类号: C12P7/40 C12N9/80 C12N15/87

    摘要: The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

    摘要翻译: 本发明涉及具有改进的基板接受性的新腈水解酶,获得它们的方法和使用上述腈水解酶。 所述腈水解酶由多肽序列编码,其在与野生型产碱杆菌粪便中的位置296相对应的位置包含不是酪氨酸的氨基酸。 本发明进一步涉及编码所述腈水解酶的核酸序列和氨基酸序列,包含所述核酸序列,包含核酸序列或exPression构建体的载体的表达构建体,包括核酸序列,表达构建体或生物体 向量 本发明还涉及从外消旋腈制备羧酸,优选取代的手性羧酸的方法。

    L-rhamnose-inducible expression systems
    10.
    发明申请
    L-rhamnose-inducible expression systems 有权
    L-鼠李糖诱导型表达系统

    公开(公告)号:US20060014291A1

    公开(公告)日:2006-01-19

    申请号:US10537075

    申请日:2003-11-27

    IPC分类号: C12N15/74 C12N1/21

    CPC分类号: C12N15/63 C12N15/70

    摘要: The present invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells, where at least one DNA construct which is capable of episomal replication in said host cells and which comprises a nucleic acid sequence to be expressed under the transcriptional control of an L-rhamnose-inducible promoter, where said promoter is heterologous with regard to said nucleic acid sequence, is introduced into said host cells and the expression of said nucleic acid sequence is induced by addition of L-rhamnose, wherein the prokaryotic host cell is at least deficient with regard to an L-rhamnose isomerase.

    摘要翻译: 本发明涉及用于在原核宿主细胞中表达核酸序列的方法,其中至少一种能够在所述宿主细胞中具有游离复制的DNA构建体,其包含在L-核苷酸的转录控制下表达的核酸序列, 将所述启动子相对于所述核酸序列异源的鼠李糖诱导型启动子引入所述宿主细胞,并通过加入L-鼠李糖诱导所述核酸序列的表达,其中所述原核宿主细胞至少是缺乏的 关于L-鼠李糖异构酶。